Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoH and nuoI are separated by 11 nucleotides nuoI and nuoJ are separated by 9 nucleotides nuoJ and nuoK are separated by 4 nucleotides nuoK and nuoL overlap by 4 nucleotides
COO64_RS24300: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 71,782 to 72,789
nuoH
COO64_RS24305: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 72,801 to 73,349
nuoI
COO64_RS24310: nuoJ - NADH-quinone oxidoreductase subunit J, at 73,359 to 73,859
nuoJ
COO64_RS24315: nuoK - NADH-quinone oxidoreductase subunit NuoK, at 73,864 to 74,172
nuoK
COO64_RS24320: nuoL - NADH-quinone oxidoreductase subunit L, at 74,169 to 76,022
nuoL
Position (kb) 73
74 Strain fitness (log2 ratio) -2
-1
0
1
2 at 72.470 kb on - strand, within nuoH at 72.508 kb on + strand, within nuoH at 72.508 kb on + strand, within nuoH at 72.509 kb on - strand, within nuoH at 72.509 kb on - strand, within nuoH at 72.509 kb on - strand, within nuoH at 72.509 kb on - strand, within nuoH at 72.509 kb on - strand, within nuoH at 72.676 kb on + strand, within nuoH at 72.677 kb on - strand, within nuoH at 72.810 kb on + strand at 72.813 kb on - strand at 72.813 kb on - strand at 72.813 kb on - strand at 72.827 kb on + strand at 73.201 kb on - strand, within nuoI at 73.201 kb on - strand, within nuoI at 73.248 kb on + strand, within nuoI at 73.248 kb on + strand, within nuoI at 73.248 kb on + strand, within nuoI at 73.249 kb on - strand, within nuoI at 73.257 kb on + strand, within nuoI at 73.271 kb on + strand, within nuoI at 73.271 kb on + strand, within nuoI at 73.271 kb on + strand, within nuoI at 73.272 kb on - strand, within nuoI at 73.272 kb on - strand, within nuoI at 73.272 kb on - strand, within nuoI at 73.375 kb on - strand at 73.449 kb on + strand, within nuoJ at 73.683 kb on + strand, within nuoJ at 73.761 kb on + strand, within nuoJ at 73.762 kb on - strand, within nuoJ at 73.762 kb on - strand, within nuoJ at 73.762 kb on - strand, within nuoJ at 73.770 kb on + strand, within nuoJ at 73.857 kb on + strand at 73.858 kb on - strand at 74.119 kb on + strand, within nuoK at 74.120 kb on - strand, within nuoK at 74.177 kb on + strand at 74.178 kb on - strand at 74.195 kb on - strand at 74.307 kb on + strand at 74.307 kb on + strand at 74.393 kb on + strand, within nuoL at 74.393 kb on + strand, within nuoL at 74.413 kb on - strand, within nuoL at 74.491 kb on - strand, within nuoL at 74.529 kb on + strand, within nuoL at 74.592 kb on + strand, within nuoL at 74.592 kb on + strand, within nuoL at 74.593 kb on - strand, within nuoL at 74.626 kb on - strand, within nuoL at 74.643 kb on + strand, within nuoL at 74.644 kb on - strand, within nuoL at 74.646 kb on + strand, within nuoL at 74.646 kb on + strand, within nuoL at 74.646 kb on + strand, within nuoL at 74.780 kb on - strand, within nuoL
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 72,470 - nuoH COO64_RS24300 0.68 +1.6 72,508 + nuoH COO64_RS24300 0.72 -1.2 72,508 + nuoH COO64_RS24300 0.72 -0.0 72,509 - nuoH COO64_RS24300 0.72 +1.0 72,509 - nuoH COO64_RS24300 0.72 -0.8 72,509 - nuoH COO64_RS24300 0.72 -0.6 72,509 - nuoH COO64_RS24300 0.72 +0.4 72,509 - nuoH COO64_RS24300 0.72 +1.0 72,676 + nuoH COO64_RS24300 0.89 +0.8 72,677 - nuoH COO64_RS24300 0.89 +1.0 72,810 + -0.2 72,813 - +2.0 72,813 - -1.5 72,813 - +0.6 72,827 + +0.4 73,201 - nuoI COO64_RS24305 0.73 +1.0 73,201 - nuoI COO64_RS24305 0.73 -0.8 73,248 + nuoI COO64_RS24305 0.81 -0.7 73,248 + nuoI COO64_RS24305 0.81 -1.2 73,248 + nuoI COO64_RS24305 0.81 +1.2 73,249 - nuoI COO64_RS24305 0.82 -0.8 73,257 + nuoI COO64_RS24305 0.83 -1.6 73,271 + nuoI COO64_RS24305 0.86 -0.0 73,271 + nuoI COO64_RS24305 0.86 +0.5 73,271 + nuoI COO64_RS24305 0.86 +0.5 73,272 - nuoI COO64_RS24305 0.86 -0.6 73,272 - nuoI COO64_RS24305 0.86 +0.5 73,272 - nuoI COO64_RS24305 0.86 +1.0 73,375 - +0.6 73,449 + nuoJ COO64_RS24310 0.18 -0.6 73,683 + nuoJ COO64_RS24310 0.65 -0.0 73,761 + nuoJ COO64_RS24310 0.80 -1.2 73,762 - nuoJ COO64_RS24310 0.80 +0.5 73,762 - nuoJ COO64_RS24310 0.80 +0.6 73,762 - nuoJ COO64_RS24310 0.80 +0.4 73,770 + nuoJ COO64_RS24310 0.82 -0.4 73,857 + -0.0 73,858 - -0.8 74,119 + nuoK COO64_RS24315 0.83 +1.0 74,120 - nuoK COO64_RS24315 0.83 +0.1 74,177 + -0.0 74,178 - -0.6 74,195 - -0.6 74,307 + -1.4 74,307 + -0.4 74,393 + nuoL COO64_RS24320 0.12 -1.0 74,393 + nuoL COO64_RS24320 0.12 -0.0 74,413 - nuoL COO64_RS24320 0.13 +1.0 74,491 - nuoL COO64_RS24320 0.17 +0.7 74,529 + nuoL COO64_RS24320 0.19 -0.0 74,592 + nuoL COO64_RS24320 0.23 -1.8 74,592 + nuoL COO64_RS24320 0.23 +1.0 74,593 - nuoL COO64_RS24320 0.23 -1.4 74,626 - nuoL COO64_RS24320 0.25 +1.5 74,643 + nuoL COO64_RS24320 0.26 +1.5 74,644 - nuoL COO64_RS24320 0.26 +0.4 74,646 + nuoL COO64_RS24320 0.26 +0.4 74,646 + nuoL COO64_RS24320 0.26 +2.4 74,646 + nuoL COO64_RS24320 0.26 -0.4 74,780 - nuoL COO64_RS24320 0.33 +0.7
Or see this region's nucleotide sequence