Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS24000 and COO64_RS24005 are separated by 2 nucleotides COO64_RS24005 and COO64_RS24010 are separated by 2 nucleotides COO64_RS24010 and COO64_RS24015 are separated by 12 nucleotides
COO64_RS24000: COO64_RS24000 - S49 family peptidase, at 13,167 to 14,387
_RS24000
COO64_RS24005: COO64_RS24005 - phage portal protein, at 14,390 to 15,997
_RS24005
COO64_RS24010: COO64_RS24010 - hypothetical protein, at 16,000 to 16,215
_RS24010
COO64_RS24015: COO64_RS24015 - terminase gpA endonuclease subunit, at 16,228 to 18,237
_RS24015
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 13.406 kb on + strand, within COO64_RS24000 at 13.406 kb on + strand, within COO64_RS24000 at 13.407 kb on - strand, within COO64_RS24000 at 13.563 kb on + strand, within COO64_RS24000 at 13.564 kb on - strand, within COO64_RS24000 at 13.564 kb on - strand, within COO64_RS24000 at 13.564 kb on - strand, within COO64_RS24000 at 13.990 kb on + strand, within COO64_RS24000 at 14.202 kb on + strand, within COO64_RS24000 at 14.281 kb on - strand at 14.391 kb on + strand at 14.391 kb on + strand at 14.391 kb on + strand at 14.643 kb on + strand, within COO64_RS24005 at 14.772 kb on + strand, within COO64_RS24005 at 14.814 kb on + strand, within COO64_RS24005 at 14.814 kb on + strand, within COO64_RS24005 at 14.815 kb on - strand, within COO64_RS24005 at 14.826 kb on + strand, within COO64_RS24005 at 14.826 kb on + strand, within COO64_RS24005 at 15.118 kb on + strand, within COO64_RS24005 at 15.118 kb on + strand, within COO64_RS24005 at 15.178 kb on - strand, within COO64_RS24005 at 15.221 kb on + strand, within COO64_RS24005 at 15.221 kb on + strand, within COO64_RS24005 at 15.221 kb on + strand, within COO64_RS24005 at 15.221 kb on + strand, within COO64_RS24005 at 15.222 kb on - strand, within COO64_RS24005 at 15.222 kb on - strand, within COO64_RS24005 at 15.290 kb on - strand, within COO64_RS24005 at 15.312 kb on + strand, within COO64_RS24005 at 15.313 kb on - strand, within COO64_RS24005 at 15.330 kb on + strand, within COO64_RS24005 at 15.538 kb on - strand, within COO64_RS24005 at 15.643 kb on + strand, within COO64_RS24005 at 15.841 kb on + strand at 15.842 kb on - strand at 16.013 kb on + strand at 16.013 kb on + strand at 16.076 kb on + strand, within COO64_RS24010 at 16.077 kb on - strand, within COO64_RS24010 at 16.229 kb on + strand at 16.229 kb on + strand at 16.229 kb on + strand at 16.400 kb on + strand at 16.401 kb on - strand at 16.508 kb on + strand, within COO64_RS24015 at 16.508 kb on + strand, within COO64_RS24015 at 16.508 kb on + strand, within COO64_RS24015 at 16.508 kb on + strand, within COO64_RS24015 at 16.509 kb on - strand, within COO64_RS24015 at 16.511 kb on + strand, within COO64_RS24015 at 16.511 kb on + strand, within COO64_RS24015 at 16.512 kb on - strand, within COO64_RS24015 at 16.532 kb on + strand, within COO64_RS24015 at 16.532 kb on + strand, within COO64_RS24015 at 16.533 kb on - strand, within COO64_RS24015 at 16.533 kb on - strand, within COO64_RS24015 at 16.572 kb on + strand, within COO64_RS24015 at 16.632 kb on - strand, within COO64_RS24015 at 16.632 kb on - strand, within COO64_RS24015 at 16.725 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.763 kb on + strand, within COO64_RS24015 at 16.764 kb on - strand, within COO64_RS24015 at 16.764 kb on - strand, within COO64_RS24015 at 16.764 kb on - strand, within COO64_RS24015 at 16.770 kb on - strand, within COO64_RS24015 at 16.932 kb on - strand, within COO64_RS24015 at 16.932 kb on - strand, within COO64_RS24015
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 13,406 + COO64_RS24000 0.20 +0.6 13,406 + COO64_RS24000 0.20 -1.5 13,407 - COO64_RS24000 0.20 +1.1 13,563 + COO64_RS24000 0.32 +1.0 13,564 - COO64_RS24000 0.33 -0.0 13,564 - COO64_RS24000 0.33 -0.6 13,564 - COO64_RS24000 0.33 -0.5 13,990 + COO64_RS24000 0.67 +1.0 14,202 + COO64_RS24000 0.85 +0.2 14,281 - +0.6 14,391 + +0.3 14,391 + -1.1 14,391 + -0.6 14,643 + COO64_RS24005 0.16 -0.6 14,772 + COO64_RS24005 0.24 -0.0 14,814 + COO64_RS24005 0.26 +0.1 14,814 + COO64_RS24005 0.26 +1.2 14,815 - COO64_RS24005 0.26 -0.9 14,826 + COO64_RS24005 0.27 -1.3 14,826 + COO64_RS24005 0.27 -1.8 15,118 + COO64_RS24005 0.45 -0.5 15,118 + COO64_RS24005 0.45 +0.3 15,178 - COO64_RS24005 0.49 -0.3 15,221 + COO64_RS24005 0.52 -1.6 15,221 + COO64_RS24005 0.52 -0.3 15,221 + COO64_RS24005 0.52 -1.0 15,221 + COO64_RS24005 0.52 -0.6 15,222 - COO64_RS24005 0.52 -0.2 15,222 - COO64_RS24005 0.52 -0.4 15,290 - COO64_RS24005 0.56 -2.6 15,312 + COO64_RS24005 0.57 +0.7 15,313 - COO64_RS24005 0.57 +0.3 15,330 + COO64_RS24005 0.58 -0.0 15,538 - COO64_RS24005 0.71 -0.4 15,643 + COO64_RS24005 0.78 -1.8 15,841 + +1.5 15,842 - +0.5 16,013 + -0.0 16,013 + +1.0 16,076 + COO64_RS24010 0.35 +0.2 16,077 - COO64_RS24010 0.36 +0.2 16,229 + +0.3 16,229 + +0.8 16,229 + -0.2 16,400 + +0.6 16,401 - +0.3 16,508 + COO64_RS24015 0.14 -0.0 16,508 + COO64_RS24015 0.14 -0.3 16,508 + COO64_RS24015 0.14 +0.1 16,508 + COO64_RS24015 0.14 +0.2 16,509 - COO64_RS24015 0.14 -0.9 16,511 + COO64_RS24015 0.14 +0.6 16,511 + COO64_RS24015 0.14 +0.3 16,512 - COO64_RS24015 0.14 -0.7 16,532 + COO64_RS24015 0.15 -0.4 16,532 + COO64_RS24015 0.15 +2.3 16,533 - COO64_RS24015 0.15 +1.1 16,533 - COO64_RS24015 0.15 -0.6 16,572 + COO64_RS24015 0.17 +1.4 16,632 - COO64_RS24015 0.20 -0.1 16,632 - COO64_RS24015 0.20 -0.1 16,725 + COO64_RS24015 0.25 +0.4 16,763 + COO64_RS24015 0.27 -0.3 16,763 + COO64_RS24015 0.27 -1.2 16,763 + COO64_RS24015 0.27 +0.8 16,763 + COO64_RS24015 0.27 -0.0 16,763 + COO64_RS24015 0.27 +0.4 16,763 + COO64_RS24015 0.27 +0.7 16,763 + COO64_RS24015 0.27 -0.7 16,763 + COO64_RS24015 0.27 +0.4 16,763 + COO64_RS24015 0.27 +0.1 16,763 + COO64_RS24015 0.27 -0.8 16,764 - COO64_RS24015 0.27 -0.2 16,764 - COO64_RS24015 0.27 -0.1 16,764 - COO64_RS24015 0.27 -1.4 16,770 - COO64_RS24015 0.27 -0.6 16,932 - COO64_RS24015 0.35 +0.1 16,932 - COO64_RS24015 0.35 +1.1
Or see this region's nucleotide sequence