Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS22695 and COO64_RS22700 are separated by 269 nucleotides COO64_RS22700 and bioD are separated by 107 nucleotides bioD and bioC are separated by 1 nucleotides
COO64_RS22695: COO64_RS22695 - phenylacyl-CoA dehydrogenase, at 78,941 to 80,746
_RS22695
COO64_RS22700: COO64_RS22700 - hypothetical protein, at 81,016 to 81,312
_RS22700
COO64_RS22705: bioD - dethiobiotin synthase, at 81,420 to 82,100
bioD
COO64_RS22710: bioC - malonyl-ACP O-methyltransferase BioC, at 82,102 to 82,926
bioC
Position (kb) 81
82 Strain fitness (log2 ratio) -2
-1
0
1 at 80.181 kb on + strand, within COO64_RS22695 at 80.181 kb on + strand, within COO64_RS22695 at 80.181 kb on + strand, within COO64_RS22695 at 80.181 kb on + strand, within COO64_RS22695 at 80.182 kb on - strand, within COO64_RS22695 at 80.182 kb on - strand, within COO64_RS22695 at 80.298 kb on + strand, within COO64_RS22695 at 80.298 kb on + strand, within COO64_RS22695 at 80.298 kb on + strand, within COO64_RS22695 at 80.299 kb on - strand, within COO64_RS22695 at 80.367 kb on + strand, within COO64_RS22695 at 80.367 kb on + strand, within COO64_RS22695 at 80.367 kb on + strand, within COO64_RS22695 at 80.367 kb on + strand, within COO64_RS22695 at 80.367 kb on + strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.368 kb on - strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.371 kb on + strand, within COO64_RS22695 at 80.372 kb on - strand, within COO64_RS22695 at 80.372 kb on - strand, within COO64_RS22695 at 80.372 kb on - strand, within COO64_RS22695 at 80.372 kb on - strand, within COO64_RS22695 at 80.372 kb on - strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.499 kb on + strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.500 kb on - strand, within COO64_RS22695 at 80.588 kb on + strand at 80.674 kb on - strand at 80.674 kb on - strand at 80.716 kb on + strand at 80.717 kb on - strand at 80.717 kb on - strand at 80.717 kb on - strand at 80.736 kb on + strand at 80.736 kb on + strand at 80.736 kb on + strand at 80.736 kb on + strand at 80.736 kb on + strand at 80.737 kb on - strand at 80.737 kb on - strand at 80.737 kb on - strand at 80.746 kb on + strand at 80.746 kb on + strand at 80.747 kb on - strand at 80.792 kb on - strand at 80.792 kb on - strand at 80.793 kb on + strand at 80.793 kb on + strand at 80.793 kb on + strand at 80.794 kb on - strand at 80.806 kb on + strand at 80.841 kb on - strand at 80.970 kb on + strand at 80.970 kb on + strand at 80.970 kb on + strand at 80.971 kb on - strand at 80.971 kb on - strand at 80.971 kb on - strand at 80.974 kb on + strand at 80.974 kb on + strand at 80.974 kb on + strand at 80.974 kb on + strand at 80.974 kb on + strand at 80.975 kb on - strand at 80.975 kb on - strand at 80.975 kb on - strand at 80.975 kb on - strand at 80.975 kb on - strand at 80.981 kb on + strand at 80.981 kb on + strand at 80.981 kb on + strand at 80.982 kb on - strand at 81.024 kb on - strand at 81.024 kb on - strand at 81.027 kb on + strand at 81.027 kb on + strand at 81.027 kb on + strand at 81.028 kb on - strand at 81.028 kb on - strand at 81.040 kb on - strand at 81.177 kb on - strand, within COO64_RS22700 at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.284 kb on + strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.285 kb on - strand at 81.348 kb on + strand at 81.348 kb on + strand at 81.349 kb on - strand at 81.390 kb on - strand at 81.404 kb on - strand at 81.411 kb on - strand at 81.445 kb on + strand at 81.895 kb on + strand, within bioD at 81.896 kb on - strand, within bioD at 81.896 kb on - strand, within bioD at 81.945 kb on - strand, within bioD at 82.087 kb on + strand at 82.088 kb on - strand at 82.088 kb on - strand at 82.115 kb on - strand at 82.115 kb on - strand at 82.132 kb on + strand at 82.132 kb on + strand at 82.132 kb on + strand at 82.133 kb on - strand at 82.133 kb on - strand at 82.139 kb on + strand at 82.140 kb on - strand at 82.140 kb on - strand at 82.140 kb on - strand at 82.151 kb on + strand at 82.151 kb on + strand at 82.151 kb on + strand at 82.151 kb on + strand at 82.152 kb on - strand at 82.152 kb on - strand at 82.152 kb on - strand at 82.152 kb on - strand at 82.152 kb on - strand at 82.152 kb on - strand at 82.163 kb on - strand at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.190 kb on + strand, within bioC at 82.191 kb on - strand, within bioC at 82.191 kb on - strand, within bioC at 82.191 kb on - strand, within bioC at 82.191 kb on - strand, within bioC at 82.307 kb on + strand, within bioC
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 80,181 + COO64_RS22695 0.69 +0.9 80,181 + COO64_RS22695 0.69 -0.1 80,181 + COO64_RS22695 0.69 -0.3 80,181 + COO64_RS22695 0.69 -0.1 80,182 - COO64_RS22695 0.69 -0.2 80,182 - COO64_RS22695 0.69 -1.3 80,298 + COO64_RS22695 0.75 -0.4 80,298 + COO64_RS22695 0.75 -0.5 80,298 + COO64_RS22695 0.75 -0.5 80,299 - COO64_RS22695 0.75 -1.6 80,367 + COO64_RS22695 0.79 +0.1 80,367 + COO64_RS22695 0.79 -0.2 80,367 + COO64_RS22695 0.79 -0.4 80,367 + COO64_RS22695 0.79 +0.1 80,367 + COO64_RS22695 0.79 -0.3 80,368 - COO64_RS22695 0.79 +0.6 80,368 - COO64_RS22695 0.79 -0.5 80,368 - COO64_RS22695 0.79 +0.2 80,368 - COO64_RS22695 0.79 +0.2 80,368 - COO64_RS22695 0.79 -0.3 80,368 - COO64_RS22695 0.79 +0.2 80,368 - COO64_RS22695 0.79 -0.4 80,368 - COO64_RS22695 0.79 +1.3 80,368 - COO64_RS22695 0.79 -0.8 80,368 - COO64_RS22695 0.79 +0.3 80,368 - COO64_RS22695 0.79 -0.5 80,371 + COO64_RS22695 0.79 +1.0 80,371 + COO64_RS22695 0.79 -1.3 80,371 + COO64_RS22695 0.79 -0.3 80,371 + COO64_RS22695 0.79 -0.5 80,371 + COO64_RS22695 0.79 -0.3 80,371 + COO64_RS22695 0.79 +0.6 80,371 + COO64_RS22695 0.79 -0.0 80,372 - COO64_RS22695 0.79 +0.1 80,372 - COO64_RS22695 0.79 -0.7 80,372 - COO64_RS22695 0.79 -0.0 80,372 - COO64_RS22695 0.79 -1.5 80,372 - COO64_RS22695 0.79 +0.1 80,499 + COO64_RS22695 0.86 +0.7 80,499 + COO64_RS22695 0.86 +0.2 80,499 + COO64_RS22695 0.86 -0.7 80,499 + COO64_RS22695 0.86 -0.3 80,499 + COO64_RS22695 0.86 -1.7 80,499 + COO64_RS22695 0.86 -1.1 80,499 + COO64_RS22695 0.86 +0.9 80,500 - COO64_RS22695 0.86 -0.2 80,500 - COO64_RS22695 0.86 -0.1 80,500 - COO64_RS22695 0.86 -0.3 80,500 - COO64_RS22695 0.86 -0.7 80,500 - COO64_RS22695 0.86 -0.6 80,500 - COO64_RS22695 0.86 +0.8 80,588 + +0.4 80,674 - +0.3 80,674 - -1.8 80,716 + -0.2 80,717 - -1.4 80,717 - +0.1 80,717 - -0.4 80,736 + -1.7 80,736 + -0.0 80,736 + -2.4 80,736 + +0.0 80,736 + -0.1 80,737 - -0.9 80,737 - +0.7 80,737 - +0.0 80,746 + -0.5 80,746 + +0.2 80,747 - -0.4 80,792 - +0.5 80,792 - +0.2 80,793 + -0.9 80,793 + -0.3 80,793 + -0.4 80,794 - -0.0 80,806 + -0.1 80,841 - -0.1 80,970 + +1.2 80,970 + -0.0 80,970 + +0.4 80,971 - +0.5 80,971 - +0.5 80,971 - +0.1 80,974 + -0.5 80,974 + -0.2 80,974 + -0.3 80,974 + -0.1 80,974 + -0.0 80,975 - -1.6 80,975 - -0.4 80,975 - +0.3 80,975 - +0.8 80,975 - +1.6 80,981 + -0.3 80,981 + -0.9 80,981 + -0.4 80,982 - -1.5 81,024 - -0.3 81,024 - +0.5 81,027 + +0.1 81,027 + -0.3 81,027 + -1.1 81,028 - +0.7 81,028 - -1.8 81,040 - -1.1 81,177 - COO64_RS22700 0.54 -0.8 81,284 + -0.4 81,284 + -0.3 81,284 + -0.5 81,284 + -0.5 81,284 + +0.4 81,284 + +1.2 81,284 + +0.3 81,284 + +0.7 81,285 - +1.7 81,285 - -0.6 81,285 - +0.2 81,285 - -0.2 81,285 - +0.9 81,285 - +0.0 81,285 - +0.7 81,285 - +0.4 81,285 - +0.0 81,285 - -0.3 81,285 - +0.5 81,348 + -1.5 81,348 + +0.2 81,349 - -0.8 81,390 - +0.0 81,404 - -2.2 81,411 - -0.6 81,445 + -0.5 81,895 + bioD COO64_RS22705 0.70 +0.3 81,896 - bioD COO64_RS22705 0.70 -0.7 81,896 - bioD COO64_RS22705 0.70 -0.2 81,945 - bioD COO64_RS22705 0.77 +0.3 82,087 + +1.4 82,088 - +0.7 82,088 - +0.6 82,115 - -0.1 82,115 - +0.2 82,132 + +0.2 82,132 + -1.0 82,132 + -0.0 82,133 - +0.1 82,133 - -0.5 82,139 + -0.9 82,140 - -0.6 82,140 - -0.0 82,140 - -0.1 82,151 + +1.1 82,151 + +0.2 82,151 + +0.1 82,151 + -0.3 82,152 - -1.3 82,152 - +1.3 82,152 - -0.4 82,152 - -1.0 82,152 - +0.1 82,152 - +0.6 82,163 - +0.1 82,190 + bioC COO64_RS22710 0.11 +0.7 82,190 + bioC COO64_RS22710 0.11 +0.9 82,190 + bioC COO64_RS22710 0.11 +0.3 82,190 + bioC COO64_RS22710 0.11 -1.6 82,190 + bioC COO64_RS22710 0.11 +0.3 82,190 + bioC COO64_RS22710 0.11 -1.6 82,190 + bioC COO64_RS22710 0.11 +0.1 82,190 + bioC COO64_RS22710 0.11 +1.1 82,190 + bioC COO64_RS22710 0.11 -1.3 82,191 - bioC COO64_RS22710 0.11 -1.0 82,191 - bioC COO64_RS22710 0.11 +0.7 82,191 - bioC COO64_RS22710 0.11 +0.3 82,191 - bioC COO64_RS22710 0.11 +0.9 82,307 + bioC COO64_RS22710 0.25 +0.1
Or see this region's nucleotide sequence