Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS21730 and rluB are separated by 700 nucleotides rluB and scpB are separated by 150 nucleotides scpB and COO64_RS21745 are separated by 15 nucleotides
COO64_RS21730: COO64_RS21730 - amino acid permease, at 114,473 to 115,894
_RS21730
COO64_RS21735: rluB - 23S rRNA pseudouridine(2605) synthase RluB, at 116,595 to 117,725
rluB
COO64_RS21740: scpB - SMC-Scp complex subunit ScpB, at 117,876 to 118,655
scpB
COO64_RS21745: COO64_RS21745 - ScpA family protein, at 118,671 to 119,369
_RS21745
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 115.638 kb on + strand, within COO64_RS21730 at 115.638 kb on + strand, within COO64_RS21730 at 115.638 kb on + strand, within COO64_RS21730 at 115.638 kb on + strand, within COO64_RS21730 at 115.638 kb on + strand, within COO64_RS21730 at 115.639 kb on - strand, within COO64_RS21730 at 115.639 kb on - strand, within COO64_RS21730 at 115.639 kb on - strand, within COO64_RS21730 at 115.919 kb on - strand at 115.919 kb on - strand at 115.919 kb on - strand at 115.964 kb on + strand at 115.965 kb on - strand at 115.965 kb on - strand at 115.977 kb on - strand at 116.059 kb on + strand at 116.060 kb on - strand at 116.060 kb on - strand at 116.060 kb on - strand at 116.060 kb on - strand at 116.078 kb on + strand at 116.079 kb on - strand at 116.079 kb on - strand at 116.079 kb on - strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.095 kb on + strand at 116.096 kb on - strand at 116.096 kb on - strand at 116.096 kb on - strand at 116.096 kb on - strand at 116.096 kb on - strand at 116.096 kb on - strand at 116.098 kb on - strand at 116.098 kb on - strand at 116.197 kb on + strand at 116.197 kb on + strand at 116.197 kb on + strand at 116.197 kb on + strand at 116.198 kb on - strand at 116.201 kb on + strand at 116.201 kb on + strand at 116.201 kb on + strand at 116.201 kb on + strand at 116.201 kb on + strand at 116.202 kb on - strand at 116.202 kb on - strand at 116.202 kb on - strand at 116.220 kb on + strand at 116.220 kb on + strand at 116.266 kb on + strand at 116.267 kb on - strand at 116.267 kb on - strand at 116.456 kb on + strand at 116.457 kb on - strand at 116.516 kb on + strand at 116.517 kb on - strand at 116.517 kb on - strand at 116.540 kb on - strand at 116.546 kb on + strand at 116.546 kb on + strand at 116.547 kb on - strand at 116.584 kb on - strand at 116.596 kb on + strand at 117.099 kb on + strand, within rluB at 117.099 kb on + strand, within rluB at 117.099 kb on + strand, within rluB at 117.100 kb on - strand, within rluB at 117.100 kb on - strand, within rluB at 117.100 kb on - strand, within rluB at 117.100 kb on - strand, within rluB at 117.137 kb on - strand, within rluB at 117.271 kb on + strand, within rluB at 117.271 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.475 kb on + strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.476 kb on - strand, within rluB at 117.744 kb on + strand at 117.744 kb on + strand at 117.745 kb on - strand at 117.745 kb on - strand at 117.761 kb on + strand at 117.762 kb on - strand at 117.762 kb on - strand at 117.796 kb on + strand at 117.796 kb on + strand at 117.828 kb on + strand at 117.828 kb on + strand at 118.286 kb on + strand, within scpB at 118.287 kb on - strand, within scpB at 118.287 kb on - strand, within scpB at 118.343 kb on - strand, within scpB at 118.382 kb on - strand, within scpB at 118.427 kb on - strand, within scpB at 118.567 kb on + strand, within scpB at 118.672 kb on + strand at 118.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 115,638 + COO64_RS21730 0.82 -0.2 115,638 + COO64_RS21730 0.82 -0.9 115,638 + COO64_RS21730 0.82 -0.9 115,638 + COO64_RS21730 0.82 +0.8 115,638 + COO64_RS21730 0.82 +0.1 115,639 - COO64_RS21730 0.82 -1.3 115,639 - COO64_RS21730 0.82 -0.3 115,639 - COO64_RS21730 0.82 -0.7 115,919 - +0.1 115,919 - -0.4 115,919 - -0.2 115,964 + +0.2 115,965 - +1.5 115,965 - +0.7 115,977 - -0.1 116,059 + +0.5 116,060 - +0.7 116,060 - -0.0 116,060 - -0.5 116,060 - +1.9 116,078 + -1.3 116,079 - +0.7 116,079 - -1.1 116,079 - -0.1 116,095 + -0.2 116,095 + -2.3 116,095 + -0.1 116,095 + -0.2 116,095 + +0.9 116,095 + +0.8 116,095 + +0.4 116,096 - -1.0 116,096 - +0.1 116,096 - +0.6 116,096 - +0.2 116,096 - -0.4 116,096 - +0.1 116,098 - -0.4 116,098 - -1.4 116,197 + -1.1 116,197 + +0.6 116,197 + -0.1 116,197 + -2.4 116,198 - -0.5 116,201 + +0.0 116,201 + +1.4 116,201 + +0.2 116,201 + +0.6 116,201 + -0.2 116,202 - +0.5 116,202 - -0.5 116,202 - +0.3 116,220 + +0.4 116,220 + -0.9 116,266 + -0.7 116,267 - -1.2 116,267 - -0.0 116,456 + -0.2 116,457 - -0.2 116,516 + -0.1 116,517 - -1.2 116,517 - -0.1 116,540 - -0.5 116,546 + -1.8 116,546 + +0.5 116,547 - -1.3 116,584 - +0.4 116,596 + -1.1 117,099 + rluB COO64_RS21735 0.45 -0.4 117,099 + rluB COO64_RS21735 0.45 -0.4 117,099 + rluB COO64_RS21735 0.45 -0.2 117,100 - rluB COO64_RS21735 0.45 -0.4 117,100 - rluB COO64_RS21735 0.45 -0.4 117,100 - rluB COO64_RS21735 0.45 -1.0 117,100 - rluB COO64_RS21735 0.45 -1.4 117,137 - rluB COO64_RS21735 0.48 +0.5 117,271 + rluB COO64_RS21735 0.60 +0.9 117,271 + rluB COO64_RS21735 0.60 -0.2 117,475 + rluB COO64_RS21735 0.78 -0.6 117,475 + rluB COO64_RS21735 0.78 -0.9 117,475 + rluB COO64_RS21735 0.78 -0.4 117,475 + rluB COO64_RS21735 0.78 -0.2 117,475 + rluB COO64_RS21735 0.78 -0.5 117,475 + rluB COO64_RS21735 0.78 -0.2 117,475 + rluB COO64_RS21735 0.78 +0.2 117,475 + rluB COO64_RS21735 0.78 -0.1 117,475 + rluB COO64_RS21735 0.78 -1.8 117,475 + rluB COO64_RS21735 0.78 -0.1 117,476 - rluB COO64_RS21735 0.78 -0.1 117,476 - rluB COO64_RS21735 0.78 -0.1 117,476 - rluB COO64_RS21735 0.78 +1.0 117,476 - rluB COO64_RS21735 0.78 -1.1 117,476 - rluB COO64_RS21735 0.78 +0.4 117,476 - rluB COO64_RS21735 0.78 +0.6 117,476 - rluB COO64_RS21735 0.78 -0.9 117,744 + -1.4 117,744 + -0.5 117,745 - +0.2 117,745 - -0.1 117,761 + +0.2 117,762 - -0.1 117,762 - -0.2 117,796 + -1.1 117,796 + +0.9 117,828 + -1.0 117,828 + +0.5 118,286 + scpB COO64_RS21740 0.53 +0.9 118,287 - scpB COO64_RS21740 0.53 +0.1 118,287 - scpB COO64_RS21740 0.53 +0.3 118,343 - scpB COO64_RS21740 0.60 +0.1 118,382 - scpB COO64_RS21740 0.65 -0.7 118,427 - scpB COO64_RS21740 0.71 -0.1 118,567 + scpB COO64_RS21740 0.89 -0.1 118,672 + -0.1 118,673 - +1.5
Or see this region's nucleotide sequence