Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS20915 and COO64_RS20920 are separated by 3 nucleotides COO64_RS20920 and COO64_RS20925 are separated by 202 nucleotides COO64_RS20925 and COO64_RS27115 are separated by 149 nucleotides
COO64_RS20915: COO64_RS20915 - HlyD family secretion protein, at 89,804 to 90,763
_RS20915
COO64_RS20920: COO64_RS20920 - DUF3302 domain-containing protein, at 90,767 to 91,117
_RS20920
COO64_RS20925: COO64_RS20925 - DUF4142 domain-containing protein, at 91,320 to 91,835
_RS20925
COO64_RS27115: COO64_RS27115 - hypothetical protein, at 91,985 to 92,143
_RS27115
Position (kb) 90
91
92 Strain fitness (log2 ratio) -2
-1
0
1 at 89.823 kb on + strand at 89.823 kb on + strand at 89.823 kb on + strand at 89.823 kb on + strand at 89.824 kb on - strand at 89.824 kb on - strand at 89.824 kb on - strand at 89.824 kb on - strand at 89.892 kb on + strand at 89.910 kb on + strand, within COO64_RS20915 at 90.007 kb on - strand, within COO64_RS20915 at 90.007 kb on - strand, within COO64_RS20915 at 90.174 kb on + strand, within COO64_RS20915 at 90.175 kb on - strand, within COO64_RS20915 at 90.175 kb on - strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.251 kb on + strand, within COO64_RS20915 at 90.252 kb on - strand, within COO64_RS20915 at 90.264 kb on + strand, within COO64_RS20915 at 90.264 kb on + strand, within COO64_RS20915 at 90.264 kb on + strand, within COO64_RS20915 at 90.265 kb on - strand, within COO64_RS20915 at 90.586 kb on - strand, within COO64_RS20915 at 90.586 kb on - strand, within COO64_RS20915 at 90.606 kb on + strand, within COO64_RS20915 at 90.606 kb on + strand, within COO64_RS20915 at 90.607 kb on - strand, within COO64_RS20915 at 90.618 kb on + strand, within COO64_RS20915 at 90.618 kb on + strand, within COO64_RS20915 at 90.618 kb on + strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.619 kb on - strand, within COO64_RS20915 at 90.738 kb on + strand at 90.738 kb on + strand at 90.739 kb on - strand at 91.032 kb on + strand, within COO64_RS20920 at 91.062 kb on + strand, within COO64_RS20920 at 91.107 kb on + strand at 91.107 kb on + strand at 91.108 kb on - strand at 91.108 kb on - strand at 91.125 kb on + strand at 91.159 kb on + strand at 91.160 kb on - strand at 91.160 kb on - strand at 91.160 kb on - strand at 91.268 kb on + strand at 91.447 kb on + strand, within COO64_RS20925 at 91.502 kb on - strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.587 kb on + strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.588 kb on - strand, within COO64_RS20925 at 91.829 kb on + strand at 91.885 kb on + strand at 91.885 kb on + strand at 91.885 kb on + strand at 91.885 kb on + strand at 91.985 kb on - strand at 91.985 kb on - strand at 92.033 kb on + strand, within COO64_RS27115 at 92.034 kb on - strand, within COO64_RS27115 at 92.034 kb on - strand, within COO64_RS27115
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 89,823 + -0.7 89,823 + +1.2 89,823 + +0.2 89,823 + -0.7 89,824 - -1.2 89,824 - +0.4 89,824 - -0.2 89,824 - +0.2 89,892 + -0.1 89,910 + COO64_RS20915 0.11 -0.4 90,007 - COO64_RS20915 0.21 +0.8 90,007 - COO64_RS20915 0.21 -0.1 90,174 + COO64_RS20915 0.39 +0.8 90,175 - COO64_RS20915 0.39 +0.4 90,175 - COO64_RS20915 0.39 -0.0 90,251 + COO64_RS20915 0.47 +0.3 90,251 + COO64_RS20915 0.47 +0.6 90,251 + COO64_RS20915 0.47 +0.2 90,251 + COO64_RS20915 0.47 +0.6 90,251 + COO64_RS20915 0.47 -0.4 90,251 + COO64_RS20915 0.47 +0.1 90,252 - COO64_RS20915 0.47 +0.2 90,264 + COO64_RS20915 0.48 +0.8 90,264 + COO64_RS20915 0.48 -0.3 90,264 + COO64_RS20915 0.48 +0.1 90,265 - COO64_RS20915 0.48 +0.2 90,586 - COO64_RS20915 0.81 +1.0 90,586 - COO64_RS20915 0.81 -0.1 90,606 + COO64_RS20915 0.84 -0.6 90,606 + COO64_RS20915 0.84 -0.6 90,607 - COO64_RS20915 0.84 -0.1 90,618 + COO64_RS20915 0.85 -0.2 90,618 + COO64_RS20915 0.85 +0.3 90,618 + COO64_RS20915 0.85 +0.2 90,619 - COO64_RS20915 0.85 -0.8 90,619 - COO64_RS20915 0.85 -0.1 90,619 - COO64_RS20915 0.85 +0.9 90,619 - COO64_RS20915 0.85 +0.6 90,619 - COO64_RS20915 0.85 +0.8 90,619 - COO64_RS20915 0.85 -1.0 90,738 + +0.3 90,738 + +0.5 90,739 - -0.2 91,032 + COO64_RS20920 0.75 -0.1 91,062 + COO64_RS20920 0.84 -2.1 91,107 + +0.9 91,107 + +0.6 91,108 - +0.8 91,108 - -0.7 91,125 + -0.1 91,159 + -0.1 91,160 - -1.3 91,160 - +0.5 91,160 - +0.5 91,268 + +0.4 91,447 + COO64_RS20925 0.25 +0.2 91,502 - COO64_RS20925 0.35 -0.1 91,587 + COO64_RS20925 0.52 -0.7 91,587 + COO64_RS20925 0.52 +0.1 91,587 + COO64_RS20925 0.52 -1.0 91,587 + COO64_RS20925 0.52 -0.4 91,587 + COO64_RS20925 0.52 -0.5 91,587 + COO64_RS20925 0.52 +1.0 91,587 + COO64_RS20925 0.52 -0.0 91,587 + COO64_RS20925 0.52 -1.4 91,587 + COO64_RS20925 0.52 -1.0 91,587 + COO64_RS20925 0.52 +0.7 91,588 - COO64_RS20925 0.52 -0.4 91,588 - COO64_RS20925 0.52 -0.1 91,588 - COO64_RS20925 0.52 +0.2 91,588 - COO64_RS20925 0.52 -2.0 91,588 - COO64_RS20925 0.52 +1.2 91,588 - COO64_RS20925 0.52 -2.3 91,588 - COO64_RS20925 0.52 -0.3 91,588 - COO64_RS20925 0.52 -1.3 91,829 + +0.4 91,885 + -0.4 91,885 + -0.8 91,885 + -0.1 91,885 + -1.7 91,985 - +0.1 91,985 - -0.8 92,033 + COO64_RS27115 0.30 +0.3 92,034 - COO64_RS27115 0.31 +0.3 92,034 - COO64_RS27115 0.31 -0.9
Or see this region's nucleotide sequence