Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bamC and COO64_RS20255 are separated by 0 nucleotides COO64_RS20255 and purC are separated by 29 nucleotides purC and COO64_RS20265 are separated by 93 nucleotides COO64_RS20265 and COO64_RS20270 are separated by 64 nucleotides COO64_RS20270 and COO64_RS20275 are separated by 22 nucleotides
COO64_RS20250: bamC - outer membrane protein assembly factor BamC, at 153,670 to 154,782
bamC
COO64_RS20255: COO64_RS20255 - MBL fold metallo-hydrolase, at 154,783 to 155,541
_RS20255
COO64_RS20260: purC - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 155,571 to 156,281
purC
COO64_RS20265: COO64_RS20265 - tRNA-Ser, at 156,375 to 156,464
_RS20265
COO64_RS20270: COO64_RS20270 - HNH endonuclease, at 156,529 to 157,098
_RS20270
COO64_RS20275: COO64_RS20275 - site-specific integrase, at 157,121 to 158,098
_RS20275
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 154.674 kb on + strand at 154.674 kb on + strand at 154.674 kb on + strand at 154.675 kb on - strand at 154.780 kb on + strand at 154.780 kb on + strand at 154.796 kb on + strand at 154.797 kb on - strand at 154.845 kb on + strand at 154.845 kb on + strand at 154.996 kb on + strand, within COO64_RS20255 at 154.997 kb on - strand, within COO64_RS20255 at 154.997 kb on - strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.011 kb on + strand, within COO64_RS20255 at 155.109 kb on + strand, within COO64_RS20255 at 155.152 kb on + strand, within COO64_RS20255 at 155.153 kb on - strand, within COO64_RS20255 at 155.153 kb on - strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.332 kb on + strand, within COO64_RS20255 at 155.492 kb on + strand at 155.493 kb on - strand at 155.539 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.592 kb on + strand at 155.616 kb on + strand at 155.617 kb on - strand at 155.617 kb on - strand at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.852 kb on + strand, within purC at 155.853 kb on - strand, within purC at 155.853 kb on - strand, within purC at 155.853 kb on - strand, within purC at 155.853 kb on - strand, within purC at 155.856 kb on + strand, within purC at 155.856 kb on + strand, within purC at 155.856 kb on + strand, within purC at 155.856 kb on + strand, within purC at 155.856 kb on + strand, within purC at 155.856 kb on + strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 155.857 kb on - strand, within purC at 156.115 kb on + strand, within purC at 156.243 kb on + strand at 156.243 kb on + strand at 156.244 kb on - strand at 156.280 kb on - strand at 156.488 kb on - strand at 156.488 kb on - strand at 156.567 kb on - strand at 156.567 kb on - strand at 156.592 kb on + strand, within COO64_RS20270 at 156.593 kb on - strand, within COO64_RS20270 at 156.593 kb on - strand, within COO64_RS20270 at 156.593 kb on - strand, within COO64_RS20270 at 156.599 kb on + strand, within COO64_RS20270 at 156.599 kb on + strand, within COO64_RS20270 at 156.600 kb on - strand, within COO64_RS20270 at 156.637 kb on + strand, within COO64_RS20270 at 156.637 kb on + strand, within COO64_RS20270 at 156.638 kb on - strand, within COO64_RS20270 at 156.638 kb on - strand, within COO64_RS20270 at 156.729 kb on + strand, within COO64_RS20270 at 156.729 kb on + strand, within COO64_RS20270 at 156.765 kb on + strand, within COO64_RS20270 at 156.765 kb on + strand, within COO64_RS20270 at 156.766 kb on - strand, within COO64_RS20270 at 156.766 kb on - strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.854 kb on + strand, within COO64_RS20270 at 156.855 kb on - strand, within COO64_RS20270 at 156.855 kb on - strand, within COO64_RS20270 at 156.855 kb on - strand, within COO64_RS20270 at 156.855 kb on - strand, within COO64_RS20270 at 156.881 kb on + strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.882 kb on - strand, within COO64_RS20270 at 156.911 kb on + strand, within COO64_RS20270 at 156.911 kb on + strand, within COO64_RS20270 at 156.912 kb on - strand, within COO64_RS20270 at 156.912 kb on - strand, within COO64_RS20270 at 156.912 kb on - strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.001 kb on + strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.002 kb on - strand, within COO64_RS20270 at 157.018 kb on + strand, within COO64_RS20270 at 157.019 kb on - strand, within COO64_RS20270 at 157.073 kb on + strand at 157.073 kb on + strand at 157.073 kb on + strand at 157.073 kb on + strand at 157.074 kb on - strand at 157.074 kb on - strand at 157.074 kb on - strand at 157.127 kb on + strand at 157.127 kb on + strand at 157.127 kb on + strand at 157.128 kb on - strand at 157.128 kb on - strand at 157.130 kb on + strand at 157.130 kb on + strand at 157.130 kb on + strand at 157.195 kb on + strand at 157.196 kb on - strand at 157.196 kb on - strand at 157.249 kb on + strand, within COO64_RS20275 at 157.249 kb on + strand, within COO64_RS20275 at 157.250 kb on - strand, within COO64_RS20275 at 157.250 kb on - strand, within COO64_RS20275 at 157.250 kb on - strand, within COO64_RS20275 at 157.250 kb on - strand, within COO64_RS20275
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 154,674 + -0.8 154,674 + +0.6 154,674 + -0.4 154,675 - -1.7 154,780 + +0.3 154,780 + +0.8 154,796 + -0.7 154,797 - -1.2 154,845 + -0.4 154,845 + +0.5 154,996 + COO64_RS20255 0.28 -0.4 154,997 - COO64_RS20255 0.28 -0.5 154,997 - COO64_RS20255 0.28 +0.2 155,003 + COO64_RS20255 0.29 +0.3 155,003 + COO64_RS20255 0.29 -1.5 155,003 + COO64_RS20255 0.29 -0.7 155,003 + COO64_RS20255 0.29 -1.3 155,004 - COO64_RS20255 0.29 -0.9 155,004 - COO64_RS20255 0.29 -1.2 155,004 - COO64_RS20255 0.29 -2.7 155,004 - COO64_RS20255 0.29 -1.5 155,011 + COO64_RS20255 0.30 +0.8 155,109 + COO64_RS20255 0.43 -2.0 155,152 + COO64_RS20255 0.49 -2.1 155,153 - COO64_RS20255 0.49 +0.3 155,153 - COO64_RS20255 0.49 -2.5 155,156 + COO64_RS20255 0.49 -0.2 155,156 + COO64_RS20255 0.49 +0.5 155,156 + COO64_RS20255 0.49 +0.0 155,156 + COO64_RS20255 0.49 -0.4 155,156 + COO64_RS20255 0.49 -0.5 155,156 + COO64_RS20255 0.49 -0.3 155,156 + COO64_RS20255 0.49 +0.3 155,156 + COO64_RS20255 0.49 +0.5 155,156 + COO64_RS20255 0.49 +0.0 155,157 - COO64_RS20255 0.49 -0.5 155,157 - COO64_RS20255 0.49 -1.1 155,157 - COO64_RS20255 0.49 -1.1 155,157 - COO64_RS20255 0.49 -0.9 155,157 - COO64_RS20255 0.49 -0.1 155,157 - COO64_RS20255 0.49 -3.4 155,157 - COO64_RS20255 0.49 +0.2 155,206 + COO64_RS20255 0.56 +0.0 155,206 + COO64_RS20255 0.56 -1.1 155,206 + COO64_RS20255 0.56 +0.4 155,230 + COO64_RS20255 0.59 -0.8 155,230 + COO64_RS20255 0.59 -0.1 155,230 + COO64_RS20255 0.59 +0.5 155,332 + COO64_RS20255 0.72 +1.3 155,492 + -1.0 155,493 - -0.7 155,539 + +0.0 155,553 + -1.0 155,553 + -0.7 155,553 + -1.0 155,553 + -0.3 155,553 + -0.7 155,554 - -1.4 155,554 - -0.3 155,554 - -1.3 155,554 - -2.0 155,554 - -2.6 155,554 - -1.8 155,554 - -1.9 155,592 + -1.5 155,616 + -0.3 155,617 - -1.0 155,617 - -2.0 155,852 + purC COO64_RS20260 0.40 -0.3 155,852 + purC COO64_RS20260 0.40 -1.5 155,852 + purC COO64_RS20260 0.40 +0.0 155,852 + purC COO64_RS20260 0.40 -1.9 155,852 + purC COO64_RS20260 0.40 -1.5 155,852 + purC COO64_RS20260 0.40 -0.3 155,852 + purC COO64_RS20260 0.40 -1.4 155,852 + purC COO64_RS20260 0.40 -1.0 155,853 - purC COO64_RS20260 0.40 -0.4 155,853 - purC COO64_RS20260 0.40 +0.5 155,853 - purC COO64_RS20260 0.40 -1.3 155,853 - purC COO64_RS20260 0.40 +0.0 155,856 + purC COO64_RS20260 0.40 +0.2 155,856 + purC COO64_RS20260 0.40 -0.3 155,856 + purC COO64_RS20260 0.40 -0.3 155,856 + purC COO64_RS20260 0.40 -1.0 155,856 + purC COO64_RS20260 0.40 -0.5 155,856 + purC COO64_RS20260 0.40 -1.7 155,857 - purC COO64_RS20260 0.40 +0.5 155,857 - purC COO64_RS20260 0.40 -0.7 155,857 - purC COO64_RS20260 0.40 -1.4 155,857 - purC COO64_RS20260 0.40 +0.0 155,857 - purC COO64_RS20260 0.40 -0.8 155,857 - purC COO64_RS20260 0.40 -0.8 155,857 - purC COO64_RS20260 0.40 -1.1 155,857 - purC COO64_RS20260 0.40 -0.8 155,857 - purC COO64_RS20260 0.40 -0.2 156,115 + purC COO64_RS20260 0.77 -1.0 156,243 + -0.8 156,243 + -0.8 156,244 - -0.5 156,280 - +0.2 156,488 - +0.1 156,488 - -0.4 156,567 - -0.5 156,567 - -0.2 156,592 + COO64_RS20270 0.11 +0.8 156,593 - COO64_RS20270 0.11 +0.2 156,593 - COO64_RS20270 0.11 +0.9 156,593 - COO64_RS20270 0.11 -0.5 156,599 + COO64_RS20270 0.12 -0.1 156,599 + COO64_RS20270 0.12 -0.4 156,600 - COO64_RS20270 0.12 +0.6 156,637 + COO64_RS20270 0.19 -0.2 156,637 + COO64_RS20270 0.19 +0.3 156,638 - COO64_RS20270 0.19 -1.1 156,638 - COO64_RS20270 0.19 -1.1 156,729 + COO64_RS20270 0.35 -0.4 156,729 + COO64_RS20270 0.35 +0.2 156,765 + COO64_RS20270 0.41 -0.1 156,765 + COO64_RS20270 0.41 -0.1 156,766 - COO64_RS20270 0.42 -0.2 156,766 - COO64_RS20270 0.42 -0.8 156,854 + COO64_RS20270 0.57 +1.8 156,854 + COO64_RS20270 0.57 +0.6 156,854 + COO64_RS20270 0.57 +0.0 156,854 + COO64_RS20270 0.57 +0.3 156,854 + COO64_RS20270 0.57 +0.2 156,854 + COO64_RS20270 0.57 +0.5 156,854 + COO64_RS20270 0.57 -1.0 156,854 + COO64_RS20270 0.57 +0.2 156,855 - COO64_RS20270 0.57 +0.9 156,855 - COO64_RS20270 0.57 +1.0 156,855 - COO64_RS20270 0.57 -0.3 156,855 - COO64_RS20270 0.57 -0.2 156,881 + COO64_RS20270 0.62 -0.7 156,882 - COO64_RS20270 0.62 -1.1 156,882 - COO64_RS20270 0.62 -0.8 156,882 - COO64_RS20270 0.62 +0.2 156,882 - COO64_RS20270 0.62 -0.3 156,882 - COO64_RS20270 0.62 +0.5 156,882 - COO64_RS20270 0.62 +0.8 156,911 + COO64_RS20270 0.67 +0.5 156,911 + COO64_RS20270 0.67 -0.9 156,912 - COO64_RS20270 0.67 -0.2 156,912 - COO64_RS20270 0.67 -0.2 156,912 - COO64_RS20270 0.67 -0.5 157,001 + COO64_RS20270 0.83 +1.0 157,001 + COO64_RS20270 0.83 +0.5 157,001 + COO64_RS20270 0.83 +0.0 157,001 + COO64_RS20270 0.83 +0.7 157,001 + COO64_RS20270 0.83 -0.2 157,001 + COO64_RS20270 0.83 -0.1 157,001 + COO64_RS20270 0.83 -1.1 157,001 + COO64_RS20270 0.83 -0.4 157,001 + COO64_RS20270 0.83 -0.4 157,001 + COO64_RS20270 0.83 -0.0 157,001 + COO64_RS20270 0.83 -0.4 157,001 + COO64_RS20270 0.83 -0.3 157,001 + COO64_RS20270 0.83 +0.3 157,001 + COO64_RS20270 0.83 +0.5 157,001 + COO64_RS20270 0.83 +0.4 157,001 + COO64_RS20270 0.83 +0.7 157,001 + COO64_RS20270 0.83 -0.3 157,001 + COO64_RS20270 0.83 +0.2 157,001 + COO64_RS20270 0.83 -0.2 157,001 + COO64_RS20270 0.83 +0.7 157,002 - COO64_RS20270 0.83 +0.7 157,002 - COO64_RS20270 0.83 +0.6 157,002 - COO64_RS20270 0.83 -0.7 157,002 - COO64_RS20270 0.83 -0.1 157,002 - COO64_RS20270 0.83 +0.7 157,002 - COO64_RS20270 0.83 +0.3 157,002 - COO64_RS20270 0.83 -0.7 157,002 - COO64_RS20270 0.83 +0.8 157,002 - COO64_RS20270 0.83 +1.0 157,002 - COO64_RS20270 0.83 +0.2 157,002 - COO64_RS20270 0.83 +1.2 157,002 - COO64_RS20270 0.83 +0.0 157,002 - COO64_RS20270 0.83 +0.5 157,002 - COO64_RS20270 0.83 -0.3 157,002 - COO64_RS20270 0.83 +0.8 157,002 - COO64_RS20270 0.83 -0.1 157,002 - COO64_RS20270 0.83 -1.2 157,002 - COO64_RS20270 0.83 -0.4 157,002 - COO64_RS20270 0.83 +0.3 157,002 - COO64_RS20270 0.83 -0.5 157,002 - COO64_RS20270 0.83 -0.0 157,002 - COO64_RS20270 0.83 -0.0 157,002 - COO64_RS20270 0.83 +1.4 157,002 - COO64_RS20270 0.83 +0.5 157,002 - COO64_RS20270 0.83 -0.5 157,018 + COO64_RS20270 0.86 +0.3 157,019 - COO64_RS20270 0.86 -0.0 157,073 + -0.7 157,073 + +0.0 157,073 + -0.8 157,073 + -0.6 157,074 - -0.4 157,074 - -0.6 157,074 - +1.3 157,127 + -0.3 157,127 + +0.4 157,127 + -0.7 157,128 - -0.5 157,128 - +0.2 157,130 + +0.1 157,130 + +0.1 157,130 + -0.2 157,195 + -0.1 157,196 - -1.2 157,196 - -1.0 157,249 + COO64_RS20275 0.13 -0.4 157,249 + COO64_RS20275 0.13 -0.3 157,250 - COO64_RS20275 0.13 +0.3 157,250 - COO64_RS20275 0.13 +0.4 157,250 - COO64_RS20275 0.13 +0.0 157,250 - COO64_RS20275 0.13 +0.2
Or see this region's nucleotide sequence