Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapA and bamC are separated by 17 nucleotides bamC and COO64_RS20255 are separated by 0 nucleotides COO64_RS20255 and purC are separated by 29 nucleotides
COO64_RS20245: dapA - 4-hydroxy-tetrahydrodipicolinate synthase, at 152,774 to 153,652
dapA
COO64_RS20250: bamC - outer membrane protein assembly factor BamC, at 153,670 to 154,782
bamC
COO64_RS20255: COO64_RS20255 - MBL fold metallo-hydrolase, at 154,783 to 155,541
_RS20255
COO64_RS20260: purC - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 155,571 to 156,281
purC
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 152.736 kb on + strand at 152.737 kb on - strand at 152.737 kb on - strand at 153.650 kb on + strand at 153.650 kb on + strand at 153.651 kb on - strand at 153.651 kb on - strand at 153.651 kb on - strand at 153.662 kb on + strand at 153.662 kb on + strand at 153.662 kb on + strand at 153.662 kb on + strand at 153.662 kb on + strand at 153.662 kb on + strand at 153.663 kb on - strand at 153.663 kb on - strand at 153.663 kb on - strand at 153.663 kb on - strand at 153.718 kb on - strand at 153.754 kb on + strand at 153.754 kb on + strand at 153.755 kb on - strand at 154.225 kb on + strand, within bamC at 154.225 kb on + strand, within bamC at 154.225 kb on + strand, within bamC at 154.225 kb on + strand, within bamC at 154.226 kb on - strand, within bamC at 154.226 kb on - strand, within bamC at 154.226 kb on - strand, within bamC at 154.226 kb on - strand, within bamC at 154.233 kb on + strand, within bamC at 154.546 kb on + strand, within bamC at 154.546 kb on + strand, within bamC at 154.546 kb on + strand, within bamC at 154.547 kb on - strand, within bamC at 154.547 kb on - strand, within bamC at 154.547 kb on - strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.549 kb on + strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.550 kb on - strand, within bamC at 154.674 kb on + strand at 154.674 kb on + strand at 154.674 kb on + strand at 154.675 kb on - strand at 154.780 kb on + strand at 154.780 kb on + strand at 154.796 kb on + strand at 154.797 kb on - strand at 154.845 kb on + strand at 154.845 kb on + strand at 154.996 kb on + strand, within COO64_RS20255 at 154.997 kb on - strand, within COO64_RS20255 at 154.997 kb on - strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.003 kb on + strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.004 kb on - strand, within COO64_RS20255 at 155.011 kb on + strand, within COO64_RS20255 at 155.109 kb on + strand, within COO64_RS20255 at 155.152 kb on + strand, within COO64_RS20255 at 155.153 kb on - strand, within COO64_RS20255 at 155.153 kb on - strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.156 kb on + strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.157 kb on - strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.206 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.230 kb on + strand, within COO64_RS20255 at 155.332 kb on + strand, within COO64_RS20255 at 155.492 kb on + strand at 155.493 kb on - strand at 155.539 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.553 kb on + strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.554 kb on - strand at 155.592 kb on + strand at 155.616 kb on + strand at 155.617 kb on - strand at 155.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 152,736 + -0.5 152,737 - -1.0 152,737 - -0.8 153,650 + +0.6 153,650 + -0.8 153,651 - -0.7 153,651 - -1.0 153,651 - -1.1 153,662 + +1.3 153,662 + +0.7 153,662 + -0.3 153,662 + +0.8 153,662 + +0.2 153,662 + -1.8 153,663 - -1.0 153,663 - -1.9 153,663 - -0.4 153,663 - -1.0 153,718 - +0.0 153,754 + -0.8 153,754 + -0.8 153,755 - -2.2 154,225 + bamC COO64_RS20250 0.50 +0.4 154,225 + bamC COO64_RS20250 0.50 +0.1 154,225 + bamC COO64_RS20250 0.50 -0.1 154,225 + bamC COO64_RS20250 0.50 -0.2 154,226 - bamC COO64_RS20250 0.50 -0.8 154,226 - bamC COO64_RS20250 0.50 -0.5 154,226 - bamC COO64_RS20250 0.50 +1.2 154,226 - bamC COO64_RS20250 0.50 -1.0 154,233 + bamC COO64_RS20250 0.51 -0.4 154,546 + bamC COO64_RS20250 0.79 +0.0 154,546 + bamC COO64_RS20250 0.79 +0.7 154,546 + bamC COO64_RS20250 0.79 +0.1 154,547 - bamC COO64_RS20250 0.79 -0.9 154,547 - bamC COO64_RS20250 0.79 -2.1 154,547 - bamC COO64_RS20250 0.79 -1.9 154,549 + bamC COO64_RS20250 0.79 -0.1 154,549 + bamC COO64_RS20250 0.79 -1.0 154,549 + bamC COO64_RS20250 0.79 -0.5 154,549 + bamC COO64_RS20250 0.79 +0.8 154,549 + bamC COO64_RS20250 0.79 -0.4 154,549 + bamC COO64_RS20250 0.79 -0.3 154,549 + bamC COO64_RS20250 0.79 -2.6 154,549 + bamC COO64_RS20250 0.79 -0.9 154,549 + bamC COO64_RS20250 0.79 -0.0 154,550 - bamC COO64_RS20250 0.79 -1.5 154,550 - bamC COO64_RS20250 0.79 -1.7 154,550 - bamC COO64_RS20250 0.79 -0.8 154,550 - bamC COO64_RS20250 0.79 -2.1 154,550 - bamC COO64_RS20250 0.79 -1.7 154,550 - bamC COO64_RS20250 0.79 +0.0 154,550 - bamC COO64_RS20250 0.79 -0.8 154,550 - bamC COO64_RS20250 0.79 -0.9 154,550 - bamC COO64_RS20250 0.79 -0.3 154,550 - bamC COO64_RS20250 0.79 -0.9 154,550 - bamC COO64_RS20250 0.79 -1.6 154,674 + -0.8 154,674 + +0.6 154,674 + -0.4 154,675 - -1.7 154,780 + +0.3 154,780 + +0.8 154,796 + -0.7 154,797 - -1.2 154,845 + -0.4 154,845 + +0.5 154,996 + COO64_RS20255 0.28 -0.4 154,997 - COO64_RS20255 0.28 -0.5 154,997 - COO64_RS20255 0.28 +0.2 155,003 + COO64_RS20255 0.29 +0.3 155,003 + COO64_RS20255 0.29 -1.5 155,003 + COO64_RS20255 0.29 -0.7 155,003 + COO64_RS20255 0.29 -1.3 155,004 - COO64_RS20255 0.29 -0.9 155,004 - COO64_RS20255 0.29 -1.2 155,004 - COO64_RS20255 0.29 -2.7 155,004 - COO64_RS20255 0.29 -1.5 155,011 + COO64_RS20255 0.30 +0.8 155,109 + COO64_RS20255 0.43 -2.0 155,152 + COO64_RS20255 0.49 -2.1 155,153 - COO64_RS20255 0.49 +0.3 155,153 - COO64_RS20255 0.49 -2.5 155,156 + COO64_RS20255 0.49 -0.2 155,156 + COO64_RS20255 0.49 +0.5 155,156 + COO64_RS20255 0.49 +0.0 155,156 + COO64_RS20255 0.49 -0.4 155,156 + COO64_RS20255 0.49 -0.5 155,156 + COO64_RS20255 0.49 -0.3 155,156 + COO64_RS20255 0.49 +0.3 155,156 + COO64_RS20255 0.49 +0.5 155,156 + COO64_RS20255 0.49 +0.0 155,157 - COO64_RS20255 0.49 -0.5 155,157 - COO64_RS20255 0.49 -1.1 155,157 - COO64_RS20255 0.49 -1.1 155,157 - COO64_RS20255 0.49 -0.9 155,157 - COO64_RS20255 0.49 -0.1 155,157 - COO64_RS20255 0.49 -3.4 155,157 - COO64_RS20255 0.49 +0.2 155,206 + COO64_RS20255 0.56 +0.0 155,206 + COO64_RS20255 0.56 -1.1 155,206 + COO64_RS20255 0.56 +0.4 155,230 + COO64_RS20255 0.59 -0.8 155,230 + COO64_RS20255 0.59 -0.1 155,230 + COO64_RS20255 0.59 +0.5 155,332 + COO64_RS20255 0.72 +1.3 155,492 + -1.0 155,493 - -0.7 155,539 + +0.0 155,553 + -1.0 155,553 + -0.7 155,553 + -1.0 155,553 + -0.3 155,553 + -0.7 155,554 - -1.4 155,554 - -0.3 155,554 - -1.3 155,554 - -2.0 155,554 - -2.6 155,554 - -1.8 155,554 - -1.9 155,592 + -1.5 155,616 + -0.3 155,617 - -1.0 155,617 - -2.0
Or see this region's nucleotide sequence