Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS20085 and ruvC are separated by 191 nucleotides ruvC and ruvA are separated by 108 nucleotides ruvA and ruvB are separated by 0 nucleotides
COO64_RS20085: COO64_RS20085 - YebC/PmpR family DNA-binding transcriptional regulator, at 122,402 to 123,148
_RS20085
COO64_RS20090: ruvC - crossover junction endodeoxyribonuclease RuvC, at 123,340 to 123,864
ruvC
COO64_RS20095: ruvA - Holliday junction branch migration protein RuvA, at 123,973 to 124,581
ruvA
COO64_RS20100: ruvB - Holliday junction branch migration DNA helicase RuvB, at 124,582 to 125,637
ruvB
Position (kb) 123
124 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 122.380 kb on + strand at 122.380 kb on + strand at 122.381 kb on - strand at 122.381 kb on - strand at 122.381 kb on - strand at 122.381 kb on - strand at 122.381 kb on - strand at 122.401 kb on + strand at 122.401 kb on + strand at 122.661 kb on - strand, within COO64_RS20085 at 122.661 kb on - strand, within COO64_RS20085 at 122.768 kb on - strand, within COO64_RS20085 at 122.795 kb on + strand, within COO64_RS20085 at 122.795 kb on + strand, within COO64_RS20085 at 122.795 kb on + strand, within COO64_RS20085 at 122.796 kb on - strand, within COO64_RS20085 at 122.796 kb on - strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.942 kb on + strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.943 kb on - strand, within COO64_RS20085 at 122.953 kb on + strand, within COO64_RS20085 at 122.953 kb on + strand, within COO64_RS20085 at 122.953 kb on + strand, within COO64_RS20085 at 122.953 kb on + strand, within COO64_RS20085 at 122.954 kb on - strand, within COO64_RS20085 at 122.954 kb on - strand, within COO64_RS20085 at 123.051 kb on + strand, within COO64_RS20085 at 123.051 kb on + strand, within COO64_RS20085 at 123.051 kb on + strand, within COO64_RS20085 at 123.051 kb on + strand, within COO64_RS20085 at 123.052 kb on - strand, within COO64_RS20085 at 123.052 kb on - strand, within COO64_RS20085 at 123.052 kb on - strand, within COO64_RS20085 at 123.101 kb on + strand at 123.101 kb on + strand at 123.102 kb on - strand at 123.102 kb on - strand at 123.147 kb on - strand at 123.165 kb on + strand at 123.166 kb on - strand at 123.166 kb on - strand at 123.166 kb on - strand at 123.166 kb on - strand at 123.205 kb on - strand at 123.300 kb on + strand at 123.300 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.328 kb on + strand at 123.329 kb on - strand at 123.329 kb on - strand at 123.329 kb on - strand at 123.339 kb on + strand at 123.340 kb on - strand at 123.340 kb on - strand at 123.402 kb on + strand, within ruvC at 123.421 kb on + strand, within ruvC at 123.421 kb on + strand, within ruvC at 123.421 kb on + strand, within ruvC at 123.422 kb on - strand, within ruvC at 123.422 kb on - strand, within ruvC at 123.422 kb on - strand, within ruvC at 123.439 kb on - strand, within ruvC at 123.439 kb on - strand, within ruvC at 123.643 kb on + strand, within ruvC at 123.729 kb on + strand, within ruvC at 123.729 kb on + strand, within ruvC at 123.729 kb on + strand, within ruvC at 123.795 kb on + strand, within ruvC at 123.865 kb on + strand at 123.865 kb on + strand at 123.927 kb on - strand at 123.927 kb on - strand at 124.079 kb on - strand, within ruvA at 124.079 kb on - strand, within ruvA at 124.079 kb on - strand, within ruvA at 124.153 kb on + strand, within ruvA at 124.153 kb on + strand, within ruvA at 124.154 kb on - strand, within ruvA at 124.154 kb on - strand, within ruvA at 124.269 kb on - strand, within ruvA at 124.269 kb on - strand, within ruvA at 124.269 kb on - strand, within ruvA at 124.269 kb on - strand, within ruvA at 124.323 kb on + strand, within ruvA at 124.323 kb on + strand, within ruvA at 124.323 kb on + strand, within ruvA at 124.323 kb on + strand, within ruvA at 124.483 kb on + strand, within ruvA at 124.484 kb on - strand, within ruvA at 124.579 kb on + strand at 124.579 kb on + strand at 124.579 kb on + strand at 124.579 kb on + strand at 124.579 kb on + strand at 124.580 kb on - strand at 124.580 kb on - strand at 124.672 kb on + strand at 124.673 kb on - strand at 124.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 122,380 + -0.9 122,380 + -0.3 122,381 - +0.0 122,381 - -1.1 122,381 - +0.0 122,381 - +0.1 122,381 - -1.4 122,401 + -0.2 122,401 + +1.3 122,661 - COO64_RS20085 0.35 +1.0 122,661 - COO64_RS20085 0.35 +0.1 122,768 - COO64_RS20085 0.49 -0.6 122,795 + COO64_RS20085 0.53 -0.6 122,795 + COO64_RS20085 0.53 -0.0 122,795 + COO64_RS20085 0.53 +0.3 122,796 - COO64_RS20085 0.53 +0.5 122,796 - COO64_RS20085 0.53 +0.2 122,942 + COO64_RS20085 0.72 -0.5 122,942 + COO64_RS20085 0.72 +0.2 122,942 + COO64_RS20085 0.72 +0.4 122,942 + COO64_RS20085 0.72 -1.7 122,942 + COO64_RS20085 0.72 +0.1 122,942 + COO64_RS20085 0.72 -0.8 122,942 + COO64_RS20085 0.72 +1.0 122,943 - COO64_RS20085 0.72 +0.2 122,943 - COO64_RS20085 0.72 -1.4 122,943 - COO64_RS20085 0.72 +0.2 122,943 - COO64_RS20085 0.72 -1.5 122,943 - COO64_RS20085 0.72 +0.5 122,943 - COO64_RS20085 0.72 +0.7 122,943 - COO64_RS20085 0.72 +0.6 122,943 - COO64_RS20085 0.72 -0.0 122,943 - COO64_RS20085 0.72 -0.3 122,943 - COO64_RS20085 0.72 -0.3 122,943 - COO64_RS20085 0.72 -1.3 122,953 + COO64_RS20085 0.74 +0.3 122,953 + COO64_RS20085 0.74 -0.6 122,953 + COO64_RS20085 0.74 -0.8 122,953 + COO64_RS20085 0.74 -0.2 122,954 - COO64_RS20085 0.74 -0.4 122,954 - COO64_RS20085 0.74 -0.8 123,051 + COO64_RS20085 0.87 +0.6 123,051 + COO64_RS20085 0.87 +1.7 123,051 + COO64_RS20085 0.87 -0.5 123,051 + COO64_RS20085 0.87 -0.1 123,052 - COO64_RS20085 0.87 +0.0 123,052 - COO64_RS20085 0.87 +0.7 123,052 - COO64_RS20085 0.87 -1.1 123,101 + -0.9 123,101 + -1.0 123,102 - +0.2 123,102 - +0.2 123,147 - -1.0 123,165 + +0.4 123,166 - -0.9 123,166 - +1.2 123,166 - -1.2 123,166 - +0.1 123,205 - +0.3 123,300 + +0.5 123,300 + +0.0 123,328 + -2.9 123,328 + +3.4 123,328 + +0.0 123,328 + +0.0 123,328 + +0.6 123,328 + +1.0 123,328 + -0.4 123,328 + -0.8 123,329 - -0.8 123,329 - -1.7 123,329 - -1.8 123,339 + +0.1 123,340 - -1.4 123,340 - +0.2 123,402 + ruvC COO64_RS20090 0.12 -0.9 123,421 + ruvC COO64_RS20090 0.15 -0.3 123,421 + ruvC COO64_RS20090 0.15 +0.0 123,421 + ruvC COO64_RS20090 0.15 -1.0 123,422 - ruvC COO64_RS20090 0.16 -0.8 123,422 - ruvC COO64_RS20090 0.16 +0.2 123,422 - ruvC COO64_RS20090 0.16 +0.9 123,439 - ruvC COO64_RS20090 0.19 -0.1 123,439 - ruvC COO64_RS20090 0.19 +0.8 123,643 + ruvC COO64_RS20090 0.58 -1.8 123,729 + ruvC COO64_RS20090 0.74 -1.0 123,729 + ruvC COO64_RS20090 0.74 +0.5 123,729 + ruvC COO64_RS20090 0.74 +0.2 123,795 + ruvC COO64_RS20090 0.87 -0.5 123,865 + -0.3 123,865 + +0.3 123,927 - -0.8 123,927 - -0.2 124,079 - ruvA COO64_RS20095 0.17 +0.8 124,079 - ruvA COO64_RS20095 0.17 -1.1 124,079 - ruvA COO64_RS20095 0.17 -1.3 124,153 + ruvA COO64_RS20095 0.30 +0.4 124,153 + ruvA COO64_RS20095 0.30 -1.0 124,154 - ruvA COO64_RS20095 0.30 -1.4 124,154 - ruvA COO64_RS20095 0.30 -1.4 124,269 - ruvA COO64_RS20095 0.49 -1.8 124,269 - ruvA COO64_RS20095 0.49 -0.4 124,269 - ruvA COO64_RS20095 0.49 -0.5 124,269 - ruvA COO64_RS20095 0.49 -1.5 124,323 + ruvA COO64_RS20095 0.57 +1.5 124,323 + ruvA COO64_RS20095 0.57 -2.0 124,323 + ruvA COO64_RS20095 0.57 +0.0 124,323 + ruvA COO64_RS20095 0.57 -0.2 124,483 + ruvA COO64_RS20095 0.84 -0.5 124,484 - ruvA COO64_RS20095 0.84 +0.5 124,579 + +1.0 124,579 + -1.3 124,579 + +1.0 124,579 + -0.4 124,579 + -0.5 124,580 - -1.1 124,580 - -0.5 124,672 + -0.8 124,673 - +0.0 124,673 - -0.3
Or see this region's nucleotide sequence