Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt folD and COO64_RS19120 are separated by 284 nucleotides COO64_RS19120 and COO64_RS19125 are separated by 41 nucleotides COO64_RS19125 and COO64_RS19130 are separated by 53 nucleotides COO64_RS19130 and COO64_RS19135 are separated by 84 nucleotides COO64_RS19135 and COO64_RS19140 are separated by 53 nucleotides COO64_RS19140 and COO64_RS19145 are separated by 209 nucleotides
COO64_RS19115: folD - bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD, at 146,329 to 147,183
folD
COO64_RS19120: COO64_RS19120 - tRNA-Arg, at 147,468 to 147,544
_RS19120
COO64_RS19125: COO64_RS19125 - tRNA-His, at 147,586 to 147,661
_RS19125
COO64_RS19130: COO64_RS19130 - tRNA-Leu, at 147,715 to 147,799
_RS19130
COO64_RS19135: COO64_RS19135 - tRNA-His, at 147,884 to 147,959
_RS19135
COO64_RS19140: COO64_RS19140 - tRNA-Leu, at 148,013 to 148,097
_RS19140
COO64_RS19145: COO64_RS19145 - TonB-dependent receptor, at 148,307 to 150,718
_RS19145
Position (kb) 147
148 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 147.409 kb on + strand at 147.409 kb on + strand at 147.461 kb on + strand at 147.465 kb on + strand at 147.465 kb on + strand at 147.546 kb on + strand at 147.546 kb on + strand at 147.565 kb on + strand at 147.565 kb on + strand at 147.565 kb on + strand at 147.771 kb on + strand, within COO64_RS19130 at 147.771 kb on + strand, within COO64_RS19130 at 147.848 kb on + strand at 147.849 kb on - strand at 147.881 kb on - strand at 147.881 kb on - strand at 147.881 kb on - strand at 147.892 kb on - strand, within COO64_RS19135 at 147.892 kb on - strand, within COO64_RS19135 at 147.892 kb on - strand, within COO64_RS19135 at 148.002 kb on - strand at 148.209 kb on + strand at 148.210 kb on - strand at 148.217 kb on - strand at 148.217 kb on - strand at 148.217 kb on - strand at 148.217 kb on - strand at 148.225 kb on - strand at 148.227 kb on - strand at 148.437 kb on - strand at 148.437 kb on - strand at 148.437 kb on - strand at 148.484 kb on + strand at 148.485 kb on - strand at 148.485 kb on - strand at 148.485 kb on - strand at 148.485 kb on - strand at 148.607 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.682 kb on + strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145 at 148.683 kb on - strand, within COO64_RS19145
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 147,409 + +0.5 147,409 + -1.6 147,461 + -0.2 147,465 + -1.1 147,465 + -0.5 147,546 + +2.2 147,546 + +1.1 147,565 + +2.4 147,565 + -0.4 147,565 + +0.7 147,771 + COO64_RS19130 0.66 -3.0 147,771 + COO64_RS19130 0.66 -0.7 147,848 + +0.4 147,849 - +0.4 147,881 - -0.6 147,881 - +0.5 147,881 - -1.2 147,892 - COO64_RS19135 0.11 -0.1 147,892 - COO64_RS19135 0.11 +1.4 147,892 - COO64_RS19135 0.11 +1.5 148,002 - +0.6 148,209 + +0.1 148,210 - -0.5 148,217 - -0.0 148,217 - -0.1 148,217 - -0.1 148,217 - -0.1 148,225 - -0.2 148,227 - +0.5 148,437 - +1.3 148,437 - -0.4 148,437 - +0.9 148,484 + +1.0 148,485 - -0.4 148,485 - +0.4 148,485 - +0.2 148,485 - -0.3 148,607 + COO64_RS19145 0.12 +0.2 148,682 + COO64_RS19145 0.16 -1.0 148,682 + COO64_RS19145 0.16 -0.5 148,682 + COO64_RS19145 0.16 -0.4 148,682 + COO64_RS19145 0.16 +1.3 148,682 + COO64_RS19145 0.16 +1.1 148,682 + COO64_RS19145 0.16 -0.9 148,682 + COO64_RS19145 0.16 +0.2 148,683 - COO64_RS19145 0.16 +0.6 148,683 - COO64_RS19145 0.16 -0.2 148,683 - COO64_RS19145 0.16 -1.4 148,683 - COO64_RS19145 0.16 -0.1 148,683 - COO64_RS19145 0.16 -0.1 148,683 - COO64_RS19145 0.16 -2.0 148,683 - COO64_RS19145 0.16 -0.6 148,683 - COO64_RS19145 0.16 +0.9 148,683 - COO64_RS19145 0.16 -0.6 148,683 - COO64_RS19145 0.16 -0.4 148,683 - COO64_RS19145 0.16 -1.1 148,683 - COO64_RS19145 0.16 +0.2 148,683 - COO64_RS19145 0.16 -0.4
Or see this region's nucleotide sequence