Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hemN and COO64_RS18650 are separated by 83 nucleotides COO64_RS18650 and ccoS overlap by 8 nucleotides ccoS and COO64_RS18660 are separated by 12 nucleotides
COO64_RS18645: hemN - oxygen-independent coproporphyrinogen III oxidase, at 36,214 to 37,596
hemN
COO64_RS18650: COO64_RS18650 - sulfite exporter TauE/SafE family protein, at 37,680 to 38,363
_RS18650
COO64_RS18655: ccoS - cbb3-type cytochrome oxidase assembly protein CcoS, at 38,356 to 38,568
ccoS
COO64_RS18660: COO64_RS18660 - heavy metal translocating P-type ATPase, at 38,581 to 41,034
_RS18660
Position (kb)
37
38
39 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 36.734 kb on + strand, within hemN at 36.734 kb on + strand, within hemN at 36.734 kb on + strand, within hemN at 36.735 kb on - strand, within hemN at 36.735 kb on - strand, within hemN at 36.735 kb on - strand, within hemN at 36.735 kb on - strand, within hemN at 36.740 kb on + strand, within hemN at 36.741 kb on - strand, within hemN at 36.741 kb on - strand, within hemN at 36.741 kb on - strand, within hemN at 36.860 kb on + strand, within hemN at 36.860 kb on + strand, within hemN at 36.860 kb on + strand, within hemN at 36.861 kb on - strand, within hemN at 36.861 kb on - strand, within hemN at 36.950 kb on + strand, within hemN at 36.951 kb on - strand, within hemN at 37.160 kb on + strand, within hemN at 37.160 kb on + strand, within hemN at 37.340 kb on + strand, within hemN at 37.392 kb on - strand, within hemN at 37.392 kb on - strand, within hemN at 37.394 kb on + strand, within hemN at 37.394 kb on + strand, within hemN at 37.395 kb on - strand, within hemN at 37.395 kb on - strand, within hemN at 37.395 kb on - strand, within hemN at 37.395 kb on - strand, within hemN at 37.395 kb on - strand, within hemN at 37.427 kb on + strand, within hemN at 37.427 kb on + strand, within hemN at 37.427 kb on + strand, within hemN at 37.427 kb on + strand, within hemN at 37.427 kb on + strand, within hemN at 37.428 kb on - strand, within hemN at 37.428 kb on - strand, within hemN at 37.428 kb on - strand, within hemN at 37.428 kb on - strand, within hemN at 37.428 kb on - strand, within hemN at 37.532 kb on + strand at 37.532 kb on + strand at 37.532 kb on + strand at 37.532 kb on + strand at 37.532 kb on + strand at 37.532 kb on + strand at 37.533 kb on - strand at 37.667 kb on + strand at 37.667 kb on + strand at 37.667 kb on + strand at 37.912 kb on + strand, within COO64_RS18650 at 37.912 kb on + strand, within COO64_RS18650 at 37.913 kb on - strand, within COO64_RS18650 at 37.913 kb on - strand, within COO64_RS18650 at 37.914 kb on + strand, within COO64_RS18650 at 37.914 kb on + strand, within COO64_RS18650 at 37.914 kb on + strand, within COO64_RS18650 at 37.914 kb on + strand, within COO64_RS18650 at 38.010 kb on - strand, within COO64_RS18650 at 38.074 kb on + strand, within COO64_RS18650 at 38.074 kb on + strand, within COO64_RS18650 at 38.074 kb on + strand, within COO64_RS18650 at 38.075 kb on - strand, within COO64_RS18650 at 38.209 kb on + strand, within COO64_RS18650 at 38.210 kb on - strand, within COO64_RS18650 at 38.349 kb on + strand at 38.349 kb on + strand at 38.350 kb on - strand at 38.374 kb on - strand at 38.478 kb on - strand, within ccoS at 38.478 kb on - strand, within ccoS at 38.478 kb on - strand, within ccoS at 38.478 kb on - strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.510 kb on + strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.511 kb on - strand, within ccoS at 38.555 kb on + strand at 38.556 kb on - strand at 38.556 kb on - strand at 38.556 kb on - strand at 38.754 kb on + strand at 38.754 kb on + strand at 38.755 kb on - strand at 38.755 kb on - strand at 38.756 kb on + strand at 38.756 kb on + strand at 38.756 kb on + strand at 38.757 kb on - strand at 38.757 kb on - strand at 38.904 kb on + strand, within COO64_RS18660
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 36,734 + hemN COO64_RS18645 0.38 -0.2 36,734 + hemN COO64_RS18645 0.38 +0.0 36,734 + hemN COO64_RS18645 0.38 -1.6 36,735 - hemN COO64_RS18645 0.38 -0.5 36,735 - hemN COO64_RS18645 0.38 +0.1 36,735 - hemN COO64_RS18645 0.38 +0.1 36,735 - hemN COO64_RS18645 0.38 -1.3 36,740 + hemN COO64_RS18645 0.38 -0.1 36,741 - hemN COO64_RS18645 0.38 -1.4 36,741 - hemN COO64_RS18645 0.38 +0.0 36,741 - hemN COO64_RS18645 0.38 -1.1 36,860 + hemN COO64_RS18645 0.47 -0.7 36,860 + hemN COO64_RS18645 0.47 -0.5 36,860 + hemN COO64_RS18645 0.47 -0.3 36,861 - hemN COO64_RS18645 0.47 +0.1 36,861 - hemN COO64_RS18645 0.47 +0.4 36,950 + hemN COO64_RS18645 0.53 -1.4 36,951 - hemN COO64_RS18645 0.53 -1.9 37,160 + hemN COO64_RS18645 0.68 -0.2 37,160 + hemN COO64_RS18645 0.68 -0.2 37,340 + hemN COO64_RS18645 0.81 -0.9 37,392 - hemN COO64_RS18645 0.85 +0.2 37,392 - hemN COO64_RS18645 0.85 +0.2 37,394 + hemN COO64_RS18645 0.85 +0.9 37,394 + hemN COO64_RS18645 0.85 +0.6 37,395 - hemN COO64_RS18645 0.85 -1.0 37,395 - hemN COO64_RS18645 0.85 -0.1 37,395 - hemN COO64_RS18645 0.85 -0.8 37,395 - hemN COO64_RS18645 0.85 +0.8 37,395 - hemN COO64_RS18645 0.85 -0.8 37,427 + hemN COO64_RS18645 0.88 -0.1 37,427 + hemN COO64_RS18645 0.88 -0.5 37,427 + hemN COO64_RS18645 0.88 +0.9 37,427 + hemN COO64_RS18645 0.88 -1.1 37,427 + hemN COO64_RS18645 0.88 -1.6 37,428 - hemN COO64_RS18645 0.88 -0.4 37,428 - hemN COO64_RS18645 0.88 -0.1 37,428 - hemN COO64_RS18645 0.88 -0.2 37,428 - hemN COO64_RS18645 0.88 -1.1 37,428 - hemN COO64_RS18645 0.88 -1.3 37,532 + +0.0 37,532 + -0.6 37,532 + -0.1 37,532 + -0.4 37,532 + -1.2 37,532 + +0.6 37,533 - -0.1 37,667 + -0.1 37,667 + -0.5 37,667 + -1.1 37,912 + COO64_RS18650 0.34 +0.9 37,912 + COO64_RS18650 0.34 -0.4 37,913 - COO64_RS18650 0.34 -0.1 37,913 - COO64_RS18650 0.34 -0.1 37,914 + COO64_RS18650 0.34 +0.5 37,914 + COO64_RS18650 0.34 -0.6 37,914 + COO64_RS18650 0.34 -0.9 37,914 + COO64_RS18650 0.34 -0.4 38,010 - COO64_RS18650 0.48 +1.1 38,074 + COO64_RS18650 0.58 -1.2 38,074 + COO64_RS18650 0.58 -0.9 38,074 + COO64_RS18650 0.58 -0.1 38,075 - COO64_RS18650 0.58 +0.8 38,209 + COO64_RS18650 0.77 +0.5 38,210 - COO64_RS18650 0.77 -0.1 38,349 + -1.5 38,349 + +0.4 38,350 - +1.4 38,374 - -0.4 38,478 - ccoS COO64_RS18655 0.57 -1.8 38,478 - ccoS COO64_RS18655 0.57 -0.6 38,478 - ccoS COO64_RS18655 0.57 +0.7 38,478 - ccoS COO64_RS18655 0.57 +1.2 38,510 + ccoS COO64_RS18655 0.72 +0.9 38,510 + ccoS COO64_RS18655 0.72 +0.2 38,510 + ccoS COO64_RS18655 0.72 -1.4 38,510 + ccoS COO64_RS18655 0.72 +0.2 38,510 + ccoS COO64_RS18655 0.72 +0.9 38,510 + ccoS COO64_RS18655 0.72 +0.4 38,510 + ccoS COO64_RS18655 0.72 +1.2 38,510 + ccoS COO64_RS18655 0.72 -2.3 38,511 - ccoS COO64_RS18655 0.73 +1.4 38,511 - ccoS COO64_RS18655 0.73 +0.6 38,511 - ccoS COO64_RS18655 0.73 -0.4 38,511 - ccoS COO64_RS18655 0.73 -0.2 38,511 - ccoS COO64_RS18655 0.73 -0.6 38,511 - ccoS COO64_RS18655 0.73 -0.9 38,511 - ccoS COO64_RS18655 0.73 -1.2 38,511 - ccoS COO64_RS18655 0.73 +0.4 38,555 + +1.9 38,556 - +0.9 38,556 - -1.4 38,556 - -1.5 38,754 + +0.2 38,754 + -0.4 38,755 - -0.5 38,755 - +1.9 38,756 + +0.9 38,756 + +0.5 38,756 + +1.9 38,757 - -0.6 38,757 - -0.4 38,904 + COO64_RS18660 0.13 -0.9
Or see this region's nucleotide sequence