Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS18425 and COO64_RS18430 are separated by 50 nucleotides COO64_RS18430 and pbpC are separated by 104 nucleotides
COO64_RS18425: COO64_RS18425 - alpha-2-macroglobulin, at 221,462 to 226,417
_RS18425
COO64_RS18430: COO64_RS18430 - hypothetical protein, at 226,468 to 226,761
_RS18430
COO64_RS18435: pbpC - peptidoglycan glycosyltransferase PbpC, at 226,866 to 229,220
pbpC
Position (kb) 226
227 Strain fitness (log2 ratio) -2
-1
0
1
2 at 225.480 kb on + strand, within COO64_RS18425 at 225.480 kb on + strand, within COO64_RS18425 at 225.481 kb on - strand, within COO64_RS18425 at 225.481 kb on - strand, within COO64_RS18425 at 225.991 kb on + strand at 225.991 kb on + strand at 225.991 kb on + strand at 225.992 kb on - strand at 226.244 kb on + strand at 226.245 kb on - strand at 226.245 kb on - strand at 226.249 kb on - strand at 226.268 kb on + strand at 226.268 kb on + strand at 226.268 kb on + strand at 226.269 kb on - strand at 226.269 kb on - strand at 226.271 kb on + strand at 226.271 kb on + strand at 226.271 kb on + strand at 226.271 kb on + strand at 226.272 kb on - strand at 226.292 kb on + strand at 226.293 kb on - strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.322 kb on + strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.323 kb on - strand at 226.367 kb on + strand at 226.367 kb on + strand at 226.367 kb on + strand at 226.368 kb on - strand at 226.368 kb on - strand at 226.401 kb on + strand at 226.461 kb on + strand at 226.462 kb on - strand at 226.462 kb on - strand at 226.462 kb on - strand at 226.462 kb on - strand at 226.462 kb on - strand at 226.462 kb on - strand at 226.525 kb on + strand, within COO64_RS18430 at 226.525 kb on + strand, within COO64_RS18430 at 226.526 kb on - strand, within COO64_RS18430 at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.753 kb on + strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.754 kb on - strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.761 kb on + strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.762 kb on - strand at 226.842 kb on + strand at 226.842 kb on + strand at 226.842 kb on + strand at 226.842 kb on + strand at 226.877 kb on - strand at 226.951 kb on + strand at 226.952 kb on - strand at 227.062 kb on + strand at 227.062 kb on + strand at 227.062 kb on + strand at 227.062 kb on + strand at 227.062 kb on + strand at 227.063 kb on - strand at 227.063 kb on - strand at 227.063 kb on - strand at 227.063 kb on - strand at 227.063 kb on - strand at 227.063 kb on - strand at 227.100 kb on + strand at 227.100 kb on + strand at 227.101 kb on - strand at 227.103 kb on + strand, within pbpC at 227.104 kb on - strand, within pbpC at 227.124 kb on + strand, within pbpC at 227.125 kb on - strand, within pbpC at 227.125 kb on - strand, within pbpC at 227.125 kb on - strand, within pbpC at 227.125 kb on - strand, within pbpC at 227.142 kb on + strand, within pbpC at 227.142 kb on + strand, within pbpC at 227.143 kb on - strand, within pbpC at 227.143 kb on - strand, within pbpC at 227.143 kb on - strand, within pbpC at 227.143 kb on - strand, within pbpC at 227.143 kb on - strand, within pbpC at 227.229 kb on - strand, within pbpC at 227.229 kb on - strand, within pbpC at 227.355 kb on + strand, within pbpC at 227.355 kb on + strand, within pbpC at 227.356 kb on - strand, within pbpC at 227.415 kb on + strand, within pbpC at 227.416 kb on - strand, within pbpC at 227.416 kb on - strand, within pbpC at 227.569 kb on + strand, within pbpC at 227.569 kb on + strand, within pbpC at 227.569 kb on + strand, within pbpC at 227.570 kb on - strand, within pbpC at 227.570 kb on - strand, within pbpC
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 225,480 + COO64_RS18425 0.81 -0.0 225,480 + COO64_RS18425 0.81 -0.8 225,481 - COO64_RS18425 0.81 -0.2 225,481 - COO64_RS18425 0.81 +1.0 225,991 + +1.0 225,991 + +0.3 225,991 + +1.3 225,992 - +0.8 226,244 + +1.8 226,245 - +0.8 226,245 - -0.1 226,249 - +0.4 226,268 + +1.2 226,268 + +0.8 226,268 + -0.2 226,269 - +0.1 226,269 - +0.5 226,271 + +0.3 226,271 + -0.1 226,271 + -0.1 226,271 + -0.0 226,272 - +1.4 226,292 + -0.5 226,293 - -0.8 226,322 + +0.4 226,322 + -1.0 226,322 + -0.0 226,322 + +0.0 226,322 + +1.3 226,322 + -1.4 226,322 + -0.7 226,322 + +0.3 226,323 - -0.2 226,323 - +0.5 226,323 - +0.5 226,323 - +1.3 226,323 - +0.2 226,323 - -1.0 226,323 - +0.8 226,323 - +2.0 226,323 - +0.1 226,323 - -0.8 226,367 + +1.7 226,367 + +0.8 226,367 + -0.5 226,368 - +1.4 226,368 - -0.0 226,401 + +0.2 226,461 + +0.8 226,462 - +0.3 226,462 - +0.4 226,462 - -0.8 226,462 - +0.3 226,462 - +0.6 226,462 - -0.1 226,525 + COO64_RS18430 0.19 -0.1 226,525 + COO64_RS18430 0.19 -1.0 226,526 - COO64_RS18430 0.20 -0.9 226,753 + +0.5 226,753 + +0.4 226,753 + -0.2 226,753 + -2.0 226,753 + -0.6 226,753 + -0.3 226,753 + +1.2 226,753 + -0.5 226,753 + -0.9 226,754 - -0.3 226,754 - +0.8 226,754 - -0.4 226,754 - -0.5 226,754 - -0.5 226,754 - -0.1 226,754 - -1.5 226,761 + +0.3 226,761 + +0.1 226,761 + +0.5 226,761 + -0.4 226,761 + +1.0 226,761 + -0.7 226,761 + -0.2 226,761 + -1.1 226,761 + +0.3 226,761 + -0.1 226,761 + -1.1 226,761 + -1.2 226,761 + +0.3 226,761 + -0.5 226,761 + +1.0 226,761 + +1.8 226,761 + +0.0 226,761 + +0.6 226,761 + +0.4 226,762 - +0.4 226,762 - +0.0 226,762 - +0.8 226,762 - +0.9 226,762 - +0.2 226,762 - -0.0 226,762 - -1.1 226,762 - -0.6 226,762 - -1.9 226,762 - +1.2 226,762 - +0.8 226,762 - -0.5 226,762 - -2.0 226,762 - +0.1 226,842 + -0.0 226,842 + +0.6 226,842 + -0.2 226,842 + -0.2 226,877 - -1.5 226,951 + -0.4 226,952 - +0.0 227,062 + +1.6 227,062 + -0.3 227,062 + -0.1 227,062 + +0.8 227,062 + +0.7 227,063 - -0.4 227,063 - +0.9 227,063 - -0.5 227,063 - -1.8 227,063 - -0.5 227,063 - +0.3 227,100 + +0.7 227,100 + +0.3 227,101 - -0.5 227,103 + pbpC COO64_RS18435 0.10 +0.5 227,104 - pbpC COO64_RS18435 0.10 +0.6 227,124 + pbpC COO64_RS18435 0.11 +1.5 227,125 - pbpC COO64_RS18435 0.11 -0.5 227,125 - pbpC COO64_RS18435 0.11 -0.5 227,125 - pbpC COO64_RS18435 0.11 -0.4 227,125 - pbpC COO64_RS18435 0.11 -0.6 227,142 + pbpC COO64_RS18435 0.12 +0.0 227,142 + pbpC COO64_RS18435 0.12 +0.1 227,143 - pbpC COO64_RS18435 0.12 -1.3 227,143 - pbpC COO64_RS18435 0.12 -0.5 227,143 - pbpC COO64_RS18435 0.12 +0.0 227,143 - pbpC COO64_RS18435 0.12 +0.7 227,143 - pbpC COO64_RS18435 0.12 -0.2 227,229 - pbpC COO64_RS18435 0.15 +0.6 227,229 - pbpC COO64_RS18435 0.15 -1.1 227,355 + pbpC COO64_RS18435 0.21 +0.5 227,355 + pbpC COO64_RS18435 0.21 -0.9 227,356 - pbpC COO64_RS18435 0.21 +0.2 227,415 + pbpC COO64_RS18435 0.23 -0.8 227,416 - pbpC COO64_RS18435 0.23 -0.1 227,416 - pbpC COO64_RS18435 0.23 -0.3 227,569 + pbpC COO64_RS18435 0.30 -1.2 227,569 + pbpC COO64_RS18435 0.30 -0.3 227,569 + pbpC COO64_RS18435 0.30 +0.8 227,570 - pbpC COO64_RS18435 0.30 +0.0 227,570 - pbpC COO64_RS18435 0.30 +1.2
Or see this region's nucleotide sequence