Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS18295 and purD are separated by 135 nucleotides purD and purH are separated by 169 nucleotides
COO64_RS18295: COO64_RS18295 - hybrid sensor histidine kinase/response regulator, at 188,616 to 191,387
_RS18295
COO64_RS18300: purD - phosphoribosylamine--glycine ligase, at 191,523 to 192,818
purD
COO64_RS18305: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 192,988 to 194,595
purH
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 190.532 kb on + strand, within COO64_RS18295 at 190.638 kb on - strand, within COO64_RS18295 at 190.673 kb on + strand, within COO64_RS18295 at 190.674 kb on - strand, within COO64_RS18295 at 190.771 kb on + strand, within COO64_RS18295 at 190.772 kb on - strand, within COO64_RS18295 at 190.793 kb on + strand, within COO64_RS18295 at 190.794 kb on - strand, within COO64_RS18295 at 190.807 kb on + strand, within COO64_RS18295 at 190.807 kb on + strand, within COO64_RS18295 at 190.947 kb on + strand, within COO64_RS18295 at 190.948 kb on - strand, within COO64_RS18295 at 190.948 kb on - strand, within COO64_RS18295 at 190.960 kb on + strand, within COO64_RS18295 at 190.960 kb on + strand, within COO64_RS18295 at 190.960 kb on + strand, within COO64_RS18295 at 190.961 kb on - strand, within COO64_RS18295 at 190.961 kb on - strand, within COO64_RS18295 at 190.961 kb on - strand, within COO64_RS18295 at 190.961 kb on - strand, within COO64_RS18295 at 191.020 kb on + strand, within COO64_RS18295 at 191.020 kb on + strand, within COO64_RS18295 at 191.021 kb on - strand, within COO64_RS18295 at 191.089 kb on + strand, within COO64_RS18295 at 191.089 kb on + strand, within COO64_RS18295 at 191.090 kb on - strand, within COO64_RS18295 at 191.250 kb on + strand at 191.250 kb on + strand at 191.250 kb on + strand at 191.251 kb on - strand at 191.403 kb on + strand at 191.403 kb on + strand at 191.403 kb on + strand at 191.403 kb on + strand at 191.404 kb on - strand at 191.404 kb on - strand at 191.404 kb on - strand at 191.404 kb on - strand at 191.404 kb on - strand at 191.421 kb on + strand at 191.421 kb on + strand at 191.421 kb on + strand at 191.421 kb on + strand at 191.422 kb on - strand at 191.422 kb on - strand at 191.422 kb on - strand at 191.422 kb on - strand at 191.422 kb on - strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.425 kb on + strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.426 kb on - strand at 191.524 kb on + strand at 191.525 kb on - strand at 191.525 kb on - strand at 191.582 kb on - strand at 191.621 kb on - strand at 191.621 kb on - strand at 191.673 kb on + strand, within purD at 191.673 kb on + strand, within purD at 191.674 kb on - strand, within purD at 191.735 kb on + strand, within purD at 191.736 kb on - strand, within purD at 191.788 kb on + strand, within purD at 191.789 kb on - strand, within purD at 191.789 kb on - strand, within purD at 191.789 kb on - strand, within purD at 192.011 kb on - strand, within purD at 192.025 kb on + strand, within purD at 192.025 kb on + strand, within purD at 192.026 kb on - strand, within purD at 192.026 kb on - strand, within purD at 192.125 kb on - strand, within purD at 192.421 kb on + strand, within purD at 192.422 kb on - strand, within purD at 192.457 kb on + strand, within purD at 192.458 kb on - strand, within purD at 192.804 kb on - strand at 192.853 kb on + strand at 192.853 kb on + strand at 192.854 kb on - strand at 192.854 kb on - strand at 192.881 kb on + strand at 192.887 kb on + strand at 192.887 kb on + strand at 192.887 kb on + strand at 192.887 kb on + strand at 192.887 kb on + strand at 192.887 kb on + strand at 192.888 kb on - strand at 192.888 kb on - strand at 193.277 kb on + strand, within purH at 193.658 kb on + strand, within purH at 193.658 kb on + strand, within purH at 193.658 kb on + strand, within purH at 193.659 kb on - strand, within purH at 193.659 kb on - strand, within purH at 193.671 kb on - strand, within purH at 193.671 kb on - strand, within purH at 193.809 kb on - strand, within purH at 193.809 kb on - strand, within purH
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 190,532 + COO64_RS18295 0.69 -0.8 190,638 - COO64_RS18295 0.73 -0.7 190,673 + COO64_RS18295 0.74 +0.7 190,674 - COO64_RS18295 0.74 -0.5 190,771 + COO64_RS18295 0.78 +1.3 190,772 - COO64_RS18295 0.78 +0.0 190,793 + COO64_RS18295 0.79 -0.3 190,794 - COO64_RS18295 0.79 +0.0 190,807 + COO64_RS18295 0.79 -0.8 190,807 + COO64_RS18295 0.79 +0.5 190,947 + COO64_RS18295 0.84 +1.3 190,948 - COO64_RS18295 0.84 +0.0 190,948 - COO64_RS18295 0.84 -0.3 190,960 + COO64_RS18295 0.85 +2.0 190,960 + COO64_RS18295 0.85 -1.4 190,960 + COO64_RS18295 0.85 +0.5 190,961 - COO64_RS18295 0.85 -0.5 190,961 - COO64_RS18295 0.85 -1.3 190,961 - COO64_RS18295 0.85 +0.0 190,961 - COO64_RS18295 0.85 -0.1 191,020 + COO64_RS18295 0.87 +0.0 191,020 + COO64_RS18295 0.87 +1.0 191,021 - COO64_RS18295 0.87 -0.3 191,089 + COO64_RS18295 0.89 -1.8 191,089 + COO64_RS18295 0.89 -0.1 191,090 - COO64_RS18295 0.89 -1.4 191,250 + -1.3 191,250 + -0.5 191,250 + -0.4 191,251 - +0.6 191,403 + -1.3 191,403 + +0.5 191,403 + +0.3 191,403 + -0.1 191,404 - +1.3 191,404 - -0.5 191,404 - +0.4 191,404 - +0.3 191,404 - +0.5 191,421 + -0.7 191,421 + -0.3 191,421 + -0.8 191,421 + -0.5 191,422 - +0.6 191,422 - +0.0 191,422 - -0.6 191,422 - +0.2 191,422 - -0.5 191,425 + +0.5 191,425 + -0.3 191,425 + -0.8 191,425 + +0.5 191,425 + +1.3 191,425 + +0.8 191,425 + -0.5 191,425 + +0.9 191,426 - +0.4 191,426 - -1.1 191,426 - -0.3 191,426 - -0.1 191,426 - +0.3 191,426 - +0.5 191,426 - -0.8 191,426 - -0.6 191,524 + -0.4 191,525 - -0.5 191,525 - +0.4 191,582 - -0.8 191,621 - -1.0 191,621 - -1.1 191,673 + purD COO64_RS18300 0.12 -1.0 191,673 + purD COO64_RS18300 0.12 -0.3 191,674 - purD COO64_RS18300 0.12 -1.4 191,735 + purD COO64_RS18300 0.16 -1.4 191,736 - purD COO64_RS18300 0.16 +0.2 191,788 + purD COO64_RS18300 0.20 -2.1 191,789 - purD COO64_RS18300 0.21 -1.7 191,789 - purD COO64_RS18300 0.21 -1.0 191,789 - purD COO64_RS18300 0.21 +0.5 192,011 - purD COO64_RS18300 0.38 -2.3 192,025 + purD COO64_RS18300 0.39 -0.3 192,025 + purD COO64_RS18300 0.39 -1.4 192,026 - purD COO64_RS18300 0.39 -0.1 192,026 - purD COO64_RS18300 0.39 +0.3 192,125 - purD COO64_RS18300 0.46 -0.5 192,421 + purD COO64_RS18300 0.69 +0.0 192,422 - purD COO64_RS18300 0.69 -0.4 192,457 + purD COO64_RS18300 0.72 -0.3 192,458 - purD COO64_RS18300 0.72 -2.4 192,804 - -0.5 192,853 + -1.4 192,853 + -0.4 192,854 - +0.0 192,854 - -0.2 192,881 + -1.0 192,887 + -0.1 192,887 + -0.5 192,887 + -1.8 192,887 + -1.5 192,887 + -2.1 192,887 + -0.5 192,888 - +0.3 192,888 - +0.2 193,277 + purH COO64_RS18305 0.18 +0.0 193,658 + purH COO64_RS18305 0.42 -1.0 193,658 + purH COO64_RS18305 0.42 -0.3 193,658 + purH COO64_RS18305 0.42 +2.6 193,659 - purH COO64_RS18305 0.42 -0.3 193,659 - purH COO64_RS18305 0.42 +1.0 193,671 - purH COO64_RS18305 0.42 +0.0 193,671 - purH COO64_RS18305 0.42 -1.5 193,809 - purH COO64_RS18305 0.51 -0.8 193,809 - purH COO64_RS18305 0.51 -0.8
Or see this region's nucleotide sequence