Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS18130 and cbpA are separated by 160 nucleotides cbpA and COO64_RS18140 are separated by 2 nucleotides COO64_RS18140 and COO64_RS18145 are separated by 111 nucleotides COO64_RS18145 and COO64_RS18150 are separated by 5 nucleotides
COO64_RS18130: COO64_RS18130 - Hsp70 family protein, at 157,796 to 159,061
_RS18130
COO64_RS18135: cbpA - curved DNA-binding protein, at 159,222 to 160,181
cbpA
COO64_RS18140: COO64_RS18140 - chaperone modulator CbpM, at 160,184 to 160,489
_RS18140
COO64_RS18145: COO64_RS18145 - GNAT family N-acetyltransferase, at 160,601 to 161,122
_RS18145
COO64_RS18150: COO64_RS18150 - GNAT family N-acetyltransferase, at 161,128 to 161,655
_RS18150
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2 at 158.256 kb on + strand, within COO64_RS18130 at 158.256 kb on + strand, within COO64_RS18130 at 158.257 kb on - strand, within COO64_RS18130 at 158.491 kb on + strand, within COO64_RS18130 at 158.492 kb on - strand, within COO64_RS18130 at 158.547 kb on + strand, within COO64_RS18130 at 158.547 kb on + strand, within COO64_RS18130 at 158.547 kb on + strand, within COO64_RS18130 at 158.547 kb on + strand, within COO64_RS18130 at 158.548 kb on - strand, within COO64_RS18130 at 158.575 kb on - strand, within COO64_RS18130 at 158.575 kb on - strand, within COO64_RS18130 at 158.687 kb on + strand, within COO64_RS18130 at 158.860 kb on - strand, within COO64_RS18130 at 158.871 kb on + strand, within COO64_RS18130 at 158.871 kb on + strand, within COO64_RS18130 at 158.895 kb on + strand, within COO64_RS18130 at 159.068 kb on + strand at 159.068 kb on + strand at 159.068 kb on + strand at 159.069 kb on - strand at 159.069 kb on - strand at 159.069 kb on - strand at 159.098 kb on + strand at 159.099 kb on - strand at 159.130 kb on - strand at 159.130 kb on - strand at 159.168 kb on + strand at 159.168 kb on + strand at 159.169 kb on - strand at 159.169 kb on - strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.185 kb on + strand at 159.186 kb on - strand at 159.186 kb on - strand at 159.186 kb on - strand at 159.219 kb on + strand at 159.219 kb on + strand at 159.219 kb on + strand at 159.219 kb on + strand at 159.219 kb on + strand at 159.219 kb on + strand at 159.220 kb on - strand at 159.220 kb on - strand at 159.220 kb on - strand at 159.237 kb on + strand at 159.237 kb on + strand at 159.238 kb on - strand at 159.238 kb on - strand at 159.238 kb on - strand at 159.240 kb on + strand at 159.240 kb on + strand at 159.240 kb on + strand at 159.240 kb on + strand at 159.240 kb on + strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.241 kb on - strand at 159.256 kb on + strand at 159.256 kb on + strand at 159.257 kb on - strand at 159.294 kb on + strand at 159.294 kb on + strand at 159.295 kb on - strand at 159.315 kb on + strand at 159.315 kb on + strand at 159.315 kb on + strand at 159.315 kb on + strand at 159.315 kb on + strand at 159.315 kb on + strand at 159.316 kb on - strand at 159.316 kb on - strand at 159.316 kb on - strand at 159.316 kb on - strand at 159.378 kb on + strand, within cbpA at 159.378 kb on + strand, within cbpA at 159.379 kb on - strand, within cbpA at 159.415 kb on - strand, within cbpA at 159.432 kb on + strand, within cbpA at 160.175 kb on + strand at 160.175 kb on + strand at 160.176 kb on - strand at 160.176 kb on - strand at 160.176 kb on - strand at 160.192 kb on + strand at 160.253 kb on + strand, within COO64_RS18140 at 160.253 kb on + strand, within COO64_RS18140 at 160.254 kb on - strand, within COO64_RS18140 at 160.602 kb on + strand at 160.602 kb on + strand at 160.602 kb on + strand at 160.602 kb on + strand at 160.603 kb on - strand at 160.614 kb on + strand at 160.615 kb on - strand at 160.615 kb on - strand at 160.617 kb on + strand at 160.618 kb on - strand at 160.618 kb on - strand at 160.635 kb on + strand at 160.636 kb on - strand at 160.659 kb on + strand, within COO64_RS18145 at 160.659 kb on + strand, within COO64_RS18145 at 160.659 kb on + strand, within COO64_RS18145 at 160.659 kb on + strand, within COO64_RS18145 at 160.660 kb on - strand, within COO64_RS18145 at 160.660 kb on - strand, within COO64_RS18145 at 160.660 kb on - strand, within COO64_RS18145 at 160.660 kb on - strand, within COO64_RS18145 at 160.660 kb on - strand, within COO64_RS18145 at 160.686 kb on + strand, within COO64_RS18145 at 160.687 kb on - strand, within COO64_RS18145 at 160.740 kb on + strand, within COO64_RS18145 at 160.740 kb on + strand, within COO64_RS18145 at 160.740 kb on + strand, within COO64_RS18145 at 160.740 kb on + strand, within COO64_RS18145 at 160.741 kb on - strand, within COO64_RS18145 at 160.741 kb on - strand, within COO64_RS18145 at 160.741 kb on - strand, within COO64_RS18145 at 160.741 kb on - strand, within COO64_RS18145 at 160.977 kb on + strand, within COO64_RS18145 at 160.977 kb on + strand, within COO64_RS18145 at 160.977 kb on + strand, within COO64_RS18145 at 160.978 kb on - strand, within COO64_RS18145 at 161.076 kb on + strand at 161.077 kb on - strand at 161.077 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 158,256 + COO64_RS18130 0.36 +1.6 158,256 + COO64_RS18130 0.36 -0.7 158,257 - COO64_RS18130 0.36 -0.7 158,491 + COO64_RS18130 0.55 +0.3 158,492 - COO64_RS18130 0.55 +0.5 158,547 + COO64_RS18130 0.59 +0.0 158,547 + COO64_RS18130 0.59 -0.0 158,547 + COO64_RS18130 0.59 -0.3 158,547 + COO64_RS18130 0.59 -1.0 158,548 - COO64_RS18130 0.59 -0.7 158,575 - COO64_RS18130 0.62 +0.2 158,575 - COO64_RS18130 0.62 +1.3 158,687 + COO64_RS18130 0.70 +0.5 158,860 - COO64_RS18130 0.84 -0.8 158,871 + COO64_RS18130 0.85 +0.2 158,871 + COO64_RS18130 0.85 -0.8 158,895 + COO64_RS18130 0.87 +0.0 159,068 + -0.8 159,068 + -0.5 159,068 + -0.2 159,069 - +1.4 159,069 - +0.5 159,069 - -0.3 159,098 + +1.3 159,099 - -1.0 159,130 - -0.2 159,130 - -0.2 159,168 + -1.0 159,168 + -0.3 159,169 - -0.4 159,169 - -0.1 159,185 + -0.5 159,185 + +1.2 159,185 + +0.3 159,185 + -0.6 159,185 + +0.3 159,185 + -1.2 159,185 + -0.1 159,185 + +0.4 159,186 - +0.5 159,186 - -0.2 159,186 - -0.6 159,219 + -0.4 159,219 + -0.1 159,219 + -0.1 159,219 + -1.1 159,219 + -0.5 159,219 + -0.5 159,220 - -0.2 159,220 - +0.7 159,220 - +0.3 159,237 + -1.3 159,237 + +0.8 159,238 - +0.8 159,238 - -0.7 159,238 - -1.8 159,240 + -0.5 159,240 + +0.2 159,240 + -0.7 159,240 + -0.1 159,240 + +0.2 159,241 - -0.5 159,241 - +0.2 159,241 - -0.9 159,241 - +2.2 159,241 - -0.1 159,241 - -0.5 159,241 - -0.4 159,256 + +0.1 159,256 + -0.8 159,257 - +0.3 159,294 + -0.1 159,294 + -0.4 159,295 - -0.1 159,315 + -2.2 159,315 + -1.5 159,315 + -0.5 159,315 + +2.4 159,315 + +0.0 159,315 + -0.2 159,316 - -0.8 159,316 - -1.0 159,316 - +1.2 159,316 - -0.5 159,378 + cbpA COO64_RS18135 0.16 -0.8 159,378 + cbpA COO64_RS18135 0.16 -0.5 159,379 - cbpA COO64_RS18135 0.16 -1.1 159,415 - cbpA COO64_RS18135 0.20 -0.0 159,432 + cbpA COO64_RS18135 0.22 +0.0 160,175 + -0.6 160,175 + -0.0 160,176 - +0.5 160,176 - -0.2 160,176 - +1.3 160,192 + +0.5 160,253 + COO64_RS18140 0.23 +0.1 160,253 + COO64_RS18140 0.23 -0.3 160,254 - COO64_RS18140 0.23 -0.2 160,602 + +0.4 160,602 + +0.5 160,602 + +0.3 160,602 + +0.5 160,603 - -1.1 160,614 + -0.5 160,615 - +0.5 160,615 - +1.0 160,617 + -1.1 160,618 - +0.0 160,618 - +1.5 160,635 + +0.6 160,636 - +0.8 160,659 + COO64_RS18145 0.11 +0.7 160,659 + COO64_RS18145 0.11 -0.4 160,659 + COO64_RS18145 0.11 -0.4 160,659 + COO64_RS18145 0.11 +0.9 160,660 - COO64_RS18145 0.11 +0.7 160,660 - COO64_RS18145 0.11 +1.0 160,660 - COO64_RS18145 0.11 +0.2 160,660 - COO64_RS18145 0.11 +1.2 160,660 - COO64_RS18145 0.11 -0.2 160,686 + COO64_RS18145 0.16 +0.8 160,687 - COO64_RS18145 0.16 +0.2 160,740 + COO64_RS18145 0.27 -0.8 160,740 + COO64_RS18145 0.27 +1.9 160,740 + COO64_RS18145 0.27 +0.1 160,740 + COO64_RS18145 0.27 +0.6 160,741 - COO64_RS18145 0.27 +0.4 160,741 - COO64_RS18145 0.27 -0.5 160,741 - COO64_RS18145 0.27 -0.6 160,741 - COO64_RS18145 0.27 -0.3 160,977 + COO64_RS18145 0.72 -0.4 160,977 + COO64_RS18145 0.72 +0.3 160,977 + COO64_RS18145 0.72 +0.6 160,978 - COO64_RS18145 0.72 -0.8 161,076 + +0.2 161,077 - -0.4 161,077 - +1.1
Or see this region's nucleotide sequence