Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutL and miaA are separated by 1 nucleotides miaA and hfq are separated by 96 nucleotides hfq and hflX are separated by 12 nucleotides
COO64_RS17890: mutL - DNA mismatch repair endonuclease MutL, at 99,342 to 101,243
mutL
COO64_RS17895: miaA - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 101,245 to 102,216
miaA
COO64_RS17900: hfq - RNA chaperone Hfq, at 102,313 to 102,573
hfq
COO64_RS17905: hflX - ribosome rescue GTPase HflX, at 102,586 to 103,887
hflX
Position (kb) 101
102
103 Strain fitness (log2 ratio) -2
-1
0
1
2 at 100.315 kb on + strand, within mutL at 100.317 kb on + strand, within mutL at 100.317 kb on + strand, within mutL at 100.530 kb on + strand, within mutL at 100.536 kb on + strand, within mutL at 100.537 kb on - strand, within mutL at 100.537 kb on - strand, within mutL at 100.537 kb on - strand, within mutL at 100.542 kb on + strand, within mutL at 100.542 kb on + strand, within mutL at 100.542 kb on + strand, within mutL at 100.542 kb on + strand, within mutL at 100.543 kb on - strand, within mutL at 100.543 kb on - strand, within mutL at 100.543 kb on - strand, within mutL at 100.593 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.701 kb on + strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.702 kb on - strand, within mutL at 100.703 kb on + strand, within mutL at 100.703 kb on + strand, within mutL at 100.704 kb on - strand, within mutL at 100.704 kb on - strand, within mutL at 100.704 kb on - strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.770 kb on + strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 100.771 kb on - strand, within mutL at 101.036 kb on + strand, within mutL at 101.036 kb on + strand, within mutL at 101.037 kb on - strand, within mutL at 101.267 kb on + strand at 101.267 kb on + strand at 101.268 kb on - strand at 101.268 kb on - strand at 101.368 kb on + strand, within miaA at 101.369 kb on - strand, within miaA at 101.464 kb on + strand, within miaA at 101.464 kb on + strand, within miaA at 101.560 kb on + strand, within miaA at 101.561 kb on - strand, within miaA at 101.561 kb on - strand, within miaA at 101.561 kb on - strand, within miaA at 101.563 kb on + strand, within miaA at 101.563 kb on + strand, within miaA at 101.563 kb on + strand, within miaA at 101.563 kb on + strand, within miaA at 101.563 kb on + strand, within miaA at 101.563 kb on + strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.564 kb on - strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.706 kb on + strand, within miaA at 101.707 kb on - strand, within miaA at 101.845 kb on + strand, within miaA at 101.845 kb on + strand, within miaA at 101.845 kb on + strand, within miaA at 101.845 kb on + strand, within miaA at 101.846 kb on - strand, within miaA at 101.846 kb on - strand, within miaA at 101.847 kb on + strand, within miaA at 101.847 kb on + strand, within miaA at 101.847 kb on + strand, within miaA at 101.847 kb on + strand, within miaA at 101.847 kb on + strand, within miaA at 101.847 kb on + strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.848 kb on - strand, within miaA at 101.888 kb on + strand, within miaA at 101.888 kb on + strand, within miaA at 101.888 kb on + strand, within miaA at 101.888 kb on + strand, within miaA at 101.888 kb on + strand, within miaA at 101.888 kb on + strand, within miaA at 101.889 kb on - strand, within miaA at 101.889 kb on - strand, within miaA at 101.889 kb on - strand, within miaA at 101.889 kb on - strand, within miaA at 101.889 kb on - strand, within miaA at 101.889 kb on - strand, within miaA at 101.997 kb on + strand, within miaA at 101.997 kb on + strand, within miaA at 101.999 kb on + strand, within miaA at 102.123 kb on + strand at 102.123 kb on + strand at 102.123 kb on + strand at 102.124 kb on - strand at 102.124 kb on - strand at 102.124 kb on - strand at 102.124 kb on - strand at 102.235 kb on + strand at 102.235 kb on + strand at 102.287 kb on + strand at 102.287 kb on + strand at 102.287 kb on + strand at 102.287 kb on + strand at 102.287 kb on + strand at 102.297 kb on + strand at 102.564 kb on + strand at 102.574 kb on + strand at 102.709 kb on - strand at 102.709 kb on - strand at 102.754 kb on + strand, within hflX at 102.755 kb on - strand, within hflX at 102.785 kb on + strand, within hflX at 102.786 kb on - strand, within hflX at 102.786 kb on - strand, within hflX at 102.812 kb on + strand, within hflX at 102.813 kb on - strand, within hflX at 102.825 kb on - strand, within hflX at 102.858 kb on + strand, within hflX at 102.858 kb on + strand, within hflX at 102.859 kb on - strand, within hflX at 102.859 kb on - strand, within hflX at 102.859 kb on - strand, within hflX at 102.915 kb on + strand, within hflX at 102.916 kb on - strand, within hflX at 103.095 kb on - strand, within hflX at 103.202 kb on - strand, within hflX
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 100,315 + mutL COO64_RS17890 0.51 -0.0 100,317 + mutL COO64_RS17890 0.51 +0.7 100,317 + mutL COO64_RS17890 0.51 +0.5 100,530 + mutL COO64_RS17890 0.62 +0.6 100,536 + mutL COO64_RS17890 0.63 -0.1 100,537 - mutL COO64_RS17890 0.63 +1.3 100,537 - mutL COO64_RS17890 0.63 -1.7 100,537 - mutL COO64_RS17890 0.63 +0.5 100,542 + mutL COO64_RS17890 0.63 +2.5 100,542 + mutL COO64_RS17890 0.63 +2.0 100,542 + mutL COO64_RS17890 0.63 +0.2 100,542 + mutL COO64_RS17890 0.63 +0.9 100,543 - mutL COO64_RS17890 0.63 +1.2 100,543 - mutL COO64_RS17890 0.63 -1.4 100,543 - mutL COO64_RS17890 0.63 +0.3 100,593 + mutL COO64_RS17890 0.66 -1.1 100,701 + mutL COO64_RS17890 0.71 +1.2 100,701 + mutL COO64_RS17890 0.71 -0.1 100,701 + mutL COO64_RS17890 0.71 +0.3 100,701 + mutL COO64_RS17890 0.71 +0.1 100,701 + mutL COO64_RS17890 0.71 +1.0 100,701 + mutL COO64_RS17890 0.71 -0.2 100,701 + mutL COO64_RS17890 0.71 +0.6 100,701 + mutL COO64_RS17890 0.71 +0.2 100,702 - mutL COO64_RS17890 0.72 -0.6 100,702 - mutL COO64_RS17890 0.72 +0.0 100,702 - mutL COO64_RS17890 0.72 +0.2 100,702 - mutL COO64_RS17890 0.72 -1.4 100,702 - mutL COO64_RS17890 0.72 -0.6 100,702 - mutL COO64_RS17890 0.72 -1.0 100,702 - mutL COO64_RS17890 0.72 +0.1 100,702 - mutL COO64_RS17890 0.72 -0.3 100,702 - mutL COO64_RS17890 0.72 +0.0 100,702 - mutL COO64_RS17890 0.72 -0.4 100,702 - mutL COO64_RS17890 0.72 -0.4 100,703 + mutL COO64_RS17890 0.72 -0.6 100,703 + mutL COO64_RS17890 0.72 -0.4 100,704 - mutL COO64_RS17890 0.72 -0.2 100,704 - mutL COO64_RS17890 0.72 -1.0 100,704 - mutL COO64_RS17890 0.72 -0.0 100,770 + mutL COO64_RS17890 0.75 -0.0 100,770 + mutL COO64_RS17890 0.75 +0.8 100,770 + mutL COO64_RS17890 0.75 -0.5 100,770 + mutL COO64_RS17890 0.75 +0.8 100,770 + mutL COO64_RS17890 0.75 +0.5 100,770 + mutL COO64_RS17890 0.75 -0.1 100,770 + mutL COO64_RS17890 0.75 -0.2 100,770 + mutL COO64_RS17890 0.75 +0.1 100,770 + mutL COO64_RS17890 0.75 +1.8 100,770 + mutL COO64_RS17890 0.75 -0.3 100,770 + mutL COO64_RS17890 0.75 +0.3 100,770 + mutL COO64_RS17890 0.75 -0.1 100,771 - mutL COO64_RS17890 0.75 -1.0 100,771 - mutL COO64_RS17890 0.75 -0.4 100,771 - mutL COO64_RS17890 0.75 -0.5 100,771 - mutL COO64_RS17890 0.75 -1.0 100,771 - mutL COO64_RS17890 0.75 +0.8 100,771 - mutL COO64_RS17890 0.75 -0.1 100,771 - mutL COO64_RS17890 0.75 +0.2 101,036 + mutL COO64_RS17890 0.89 +0.1 101,036 + mutL COO64_RS17890 0.89 +1.6 101,037 - mutL COO64_RS17890 0.89 -0.8 101,267 + -1.1 101,267 + +0.0 101,268 - -0.3 101,268 - -1.1 101,368 + miaA COO64_RS17895 0.13 +1.7 101,369 - miaA COO64_RS17895 0.13 +0.3 101,464 + miaA COO64_RS17895 0.23 +0.1 101,464 + miaA COO64_RS17895 0.23 -0.3 101,560 + miaA COO64_RS17895 0.32 -0.3 101,561 - miaA COO64_RS17895 0.33 -0.3 101,561 - miaA COO64_RS17895 0.33 -0.1 101,561 - miaA COO64_RS17895 0.33 -1.9 101,563 + miaA COO64_RS17895 0.33 -0.5 101,563 + miaA COO64_RS17895 0.33 -0.5 101,563 + miaA COO64_RS17895 0.33 -0.8 101,563 + miaA COO64_RS17895 0.33 +0.7 101,563 + miaA COO64_RS17895 0.33 +0.0 101,563 + miaA COO64_RS17895 0.33 +0.0 101,564 - miaA COO64_RS17895 0.33 +0.2 101,564 - miaA COO64_RS17895 0.33 +0.2 101,564 - miaA COO64_RS17895 0.33 -1.7 101,564 - miaA COO64_RS17895 0.33 -0.5 101,564 - miaA COO64_RS17895 0.33 -2.5 101,564 - miaA COO64_RS17895 0.33 -1.3 101,564 - miaA COO64_RS17895 0.33 +0.2 101,564 - miaA COO64_RS17895 0.33 -1.4 101,564 - miaA COO64_RS17895 0.33 +0.0 101,564 - miaA COO64_RS17895 0.33 -0.5 101,564 - miaA COO64_RS17895 0.33 -1.0 101,706 + miaA COO64_RS17895 0.47 +0.7 101,706 + miaA COO64_RS17895 0.47 +1.5 101,706 + miaA COO64_RS17895 0.47 -0.7 101,706 + miaA COO64_RS17895 0.47 -2.4 101,706 + miaA COO64_RS17895 0.47 -0.5 101,706 + miaA COO64_RS17895 0.47 -0.6 101,706 + miaA COO64_RS17895 0.47 -1.5 101,706 + miaA COO64_RS17895 0.47 -1.4 101,706 + miaA COO64_RS17895 0.47 +0.9 101,707 - miaA COO64_RS17895 0.48 -0.5 101,845 + miaA COO64_RS17895 0.62 +0.0 101,845 + miaA COO64_RS17895 0.62 -0.1 101,845 + miaA COO64_RS17895 0.62 +1.6 101,845 + miaA COO64_RS17895 0.62 -0.4 101,846 - miaA COO64_RS17895 0.62 -0.5 101,846 - miaA COO64_RS17895 0.62 -1.3 101,847 + miaA COO64_RS17895 0.62 +0.0 101,847 + miaA COO64_RS17895 0.62 -0.8 101,847 + miaA COO64_RS17895 0.62 -0.3 101,847 + miaA COO64_RS17895 0.62 -1.4 101,847 + miaA COO64_RS17895 0.62 -1.3 101,847 + miaA COO64_RS17895 0.62 +0.7 101,848 - miaA COO64_RS17895 0.62 -0.5 101,848 - miaA COO64_RS17895 0.62 +0.0 101,848 - miaA COO64_RS17895 0.62 -1.5 101,848 - miaA COO64_RS17895 0.62 +1.0 101,848 - miaA COO64_RS17895 0.62 +0.5 101,848 - miaA COO64_RS17895 0.62 +0.2 101,848 - miaA COO64_RS17895 0.62 +0.5 101,848 - miaA COO64_RS17895 0.62 +0.6 101,848 - miaA COO64_RS17895 0.62 -1.0 101,848 - miaA COO64_RS17895 0.62 -0.3 101,848 - miaA COO64_RS17895 0.62 -1.3 101,888 + miaA COO64_RS17895 0.66 -0.1 101,888 + miaA COO64_RS17895 0.66 +0.0 101,888 + miaA COO64_RS17895 0.66 -0.8 101,888 + miaA COO64_RS17895 0.66 +0.0 101,888 + miaA COO64_RS17895 0.66 +0.5 101,888 + miaA COO64_RS17895 0.66 -1.4 101,889 - miaA COO64_RS17895 0.66 -0.1 101,889 - miaA COO64_RS17895 0.66 -0.8 101,889 - miaA COO64_RS17895 0.66 -0.3 101,889 - miaA COO64_RS17895 0.66 -0.4 101,889 - miaA COO64_RS17895 0.66 -1.8 101,889 - miaA COO64_RS17895 0.66 +1.0 101,997 + miaA COO64_RS17895 0.77 -0.4 101,997 + miaA COO64_RS17895 0.77 -0.1 101,999 + miaA COO64_RS17895 0.78 -0.5 102,123 + +0.7 102,123 + -0.3 102,123 + -0.8 102,124 - -0.8 102,124 - +1.5 102,124 - +0.0 102,124 - +0.5 102,235 + +0.5 102,235 + -0.8 102,287 + +2.4 102,287 + -2.0 102,287 + -0.1 102,287 + +0.5 102,287 + -1.0 102,297 + -0.4 102,564 + -1.3 102,574 + -0.3 102,709 - +0.7 102,709 - -0.0 102,754 + hflX COO64_RS17905 0.13 -0.1 102,755 - hflX COO64_RS17905 0.13 +0.3 102,785 + hflX COO64_RS17905 0.15 -0.4 102,786 - hflX COO64_RS17905 0.15 +0.3 102,786 - hflX COO64_RS17905 0.15 -0.5 102,812 + hflX COO64_RS17905 0.17 +0.4 102,813 - hflX COO64_RS17905 0.17 -0.1 102,825 - hflX COO64_RS17905 0.18 -0.0 102,858 + hflX COO64_RS17905 0.21 +0.9 102,858 + hflX COO64_RS17905 0.21 +0.3 102,859 - hflX COO64_RS17905 0.21 -1.8 102,859 - hflX COO64_RS17905 0.21 -0.3 102,859 - hflX COO64_RS17905 0.21 -0.1 102,915 + hflX COO64_RS17905 0.25 -1.0 102,916 - hflX COO64_RS17905 0.25 -0.7 103,095 - hflX COO64_RS17905 0.39 -0.1 103,202 - hflX COO64_RS17905 0.47 +0.4
Or see this region's nucleotide sequence