Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS17815 and serB are separated by 135 nucleotides serB and asd are separated by 111 nucleotides asd and COO64_RS17830 are separated by 9 nucleotides
COO64_RS17815: COO64_RS17815 - HAMP domain-containing protein, at 83,922 to 85,424
_RS17815
COO64_RS17820: serB - phosphoserine phosphatase SerB, at 85,560 to 86,774
serB
COO64_RS17825: asd - archaetidylserine decarboxylase, at 86,886 to 87,749
asd
COO64_RS17830: COO64_RS17830 - rhodanese-like domain-containing protein, at 87,759 to 88,568
_RS17830
Position (kb) 85
86
87 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 85.440 kb on - strand at 85.465 kb on + strand at 85.465 kb on + strand at 85.465 kb on + strand at 85.491 kb on + strand at 85.491 kb on + strand at 85.492 kb on - strand at 85.524 kb on + strand at 85.580 kb on + strand at 85.581 kb on - strand at 85.581 kb on - strand at 85.610 kb on + strand at 85.610 kb on + strand at 85.610 kb on + strand at 85.611 kb on - strand at 85.611 kb on - strand at 85.794 kb on - strand, within serB at 85.816 kb on - strand, within serB at 85.854 kb on - strand, within serB at 85.854 kb on - strand, within serB at 85.854 kb on - strand, within serB at 85.923 kb on + strand, within serB at 85.923 kb on + strand, within serB at 85.924 kb on - strand, within serB at 85.924 kb on - strand, within serB at 85.944 kb on - strand, within serB at 86.018 kb on - strand, within serB at 86.018 kb on - strand, within serB at 86.207 kb on - strand, within serB at 86.207 kb on - strand, within serB at 86.338 kb on + strand, within serB at 86.391 kb on + strand, within serB at 86.391 kb on + strand, within serB at 86.391 kb on + strand, within serB at 86.392 kb on - strand, within serB at 86.424 kb on + strand, within serB at 86.424 kb on + strand, within serB at 86.424 kb on + strand, within serB at 86.424 kb on + strand, within serB at 86.425 kb on - strand, within serB at 86.425 kb on - strand, within serB at 86.425 kb on - strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.463 kb on + strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.464 kb on - strand, within serB at 86.736 kb on + strand at 86.736 kb on + strand at 86.736 kb on + strand at 86.737 kb on - strand at 86.737 kb on - strand at 86.737 kb on - strand at 86.849 kb on + strand at 86.850 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 85,440 - +0.5 85,465 + -0.5 85,465 + +0.5 85,465 + +1.0 85,491 + +0.0 85,491 + +0.5 85,492 - -0.1 85,524 + +0.0 85,580 + +0.0 85,581 - -1.6 85,581 - -0.3 85,610 + -1.2 85,610 + -0.0 85,610 + -1.0 85,611 - +1.0 85,611 - -1.9 85,794 - serB COO64_RS17820 0.19 -0.3 85,816 - serB COO64_RS17820 0.21 -0.1 85,854 - serB COO64_RS17820 0.24 -1.3 85,854 - serB COO64_RS17820 0.24 -1.3 85,854 - serB COO64_RS17820 0.24 -0.9 85,923 + serB COO64_RS17820 0.30 -1.3 85,923 + serB COO64_RS17820 0.30 -1.5 85,924 - serB COO64_RS17820 0.30 -1.7 85,924 - serB COO64_RS17820 0.30 -2.1 85,944 - serB COO64_RS17820 0.32 +1.2 86,018 - serB COO64_RS17820 0.38 -1.0 86,018 - serB COO64_RS17820 0.38 +0.3 86,207 - serB COO64_RS17820 0.53 -2.7 86,207 - serB COO64_RS17820 0.53 -0.5 86,338 + serB COO64_RS17820 0.64 -1.0 86,391 + serB COO64_RS17820 0.68 -1.0 86,391 + serB COO64_RS17820 0.68 -0.6 86,391 + serB COO64_RS17820 0.68 -0.8 86,392 - serB COO64_RS17820 0.68 -1.0 86,424 + serB COO64_RS17820 0.71 +0.0 86,424 + serB COO64_RS17820 0.71 -0.3 86,424 + serB COO64_RS17820 0.71 -2.0 86,424 + serB COO64_RS17820 0.71 -2.6 86,425 - serB COO64_RS17820 0.71 -1.0 86,425 - serB COO64_RS17820 0.71 -0.3 86,425 - serB COO64_RS17820 0.71 -0.1 86,463 + serB COO64_RS17820 0.74 -0.1 86,463 + serB COO64_RS17820 0.74 -1.1 86,463 + serB COO64_RS17820 0.74 -0.8 86,463 + serB COO64_RS17820 0.74 -1.8 86,463 + serB COO64_RS17820 0.74 -2.9 86,463 + serB COO64_RS17820 0.74 -0.3 86,463 + serB COO64_RS17820 0.74 -0.2 86,463 + serB COO64_RS17820 0.74 +0.0 86,463 + serB COO64_RS17820 0.74 -0.3 86,463 + serB COO64_RS17820 0.74 -1.0 86,463 + serB COO64_RS17820 0.74 -0.7 86,464 - serB COO64_RS17820 0.74 +0.2 86,464 - serB COO64_RS17820 0.74 -1.7 86,464 - serB COO64_RS17820 0.74 -0.0 86,464 - serB COO64_RS17820 0.74 -1.1 86,464 - serB COO64_RS17820 0.74 -1.1 86,464 - serB COO64_RS17820 0.74 -0.3 86,464 - serB COO64_RS17820 0.74 -0.7 86,464 - serB COO64_RS17820 0.74 +0.7 86,464 - serB COO64_RS17820 0.74 +0.5 86,464 - serB COO64_RS17820 0.74 -0.4 86,464 - serB COO64_RS17820 0.74 -1.1 86,736 + +1.2 86,736 + -1.4 86,736 + -0.3 86,737 - +0.9 86,737 - -0.6 86,737 - -0.9 86,849 + -0.7 86,850 - +0.3
Or see this region's nucleotide sequence