Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apbC and COO64_RS16925 are separated by 162 nucleotides COO64_RS16925 and dcd are separated by 351 nucleotides dcd and COO64_RS16935 are separated by 99 nucleotides COO64_RS16935 and COO64_RS16940 are separated by 3 nucleotides
COO64_RS16920: apbC - iron-sulfur cluster carrier protein ApbC, at 141,327 to 142,421
apbC
COO64_RS16925: COO64_RS16925 - cold-shock protein, at 142,584 to 142,793
_RS16925
COO64_RS16930: dcd - dCTP deaminase, at 143,145 to 143,711
dcd
COO64_RS16935: COO64_RS16935 - hypothetical protein, at 143,811 to 144,044
_RS16935
COO64_RS16940: COO64_RS16940 - ABC transporter ATP-binding protein, at 144,048 to 144,812
_RS16940
Position (kb)
143
144 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 142.406 kb on + strand at 142.530 kb on - strand at 142.530 kb on - strand at 142.573 kb on + strand at 142.573 kb on + strand at 142.573 kb on + strand at 142.574 kb on - strand at 142.585 kb on + strand at 142.585 kb on + strand at 142.586 kb on - strand at 142.586 kb on - strand at 142.586 kb on - strand at 142.586 kb on - strand at 142.589 kb on + strand at 142.590 kb on - strand at 142.590 kb on - strand at 142.590 kb on - strand at 142.597 kb on - strand at 142.597 kb on - strand at 142.597 kb on - strand at 142.597 kb on - strand at 142.597 kb on - strand at 142.597 kb on - strand at 142.701 kb on + strand, within COO64_RS16925 at 142.701 kb on + strand, within COO64_RS16925 at 142.702 kb on - strand, within COO64_RS16925 at 142.702 kb on - strand, within COO64_RS16925 at 142.702 kb on - strand, within COO64_RS16925 at 142.702 kb on - strand, within COO64_RS16925 at 142.766 kb on + strand, within COO64_RS16925 at 142.766 kb on + strand, within COO64_RS16925 at 142.767 kb on - strand, within COO64_RS16925 at 142.767 kb on - strand, within COO64_RS16925 at 142.767 kb on - strand, within COO64_RS16925 at 142.767 kb on - strand, within COO64_RS16925 at 142.816 kb on + strand at 142.816 kb on + strand at 142.816 kb on + strand at 142.816 kb on + strand at 142.817 kb on - strand at 142.820 kb on + strand at 142.821 kb on - strand at 142.821 kb on - strand at 142.821 kb on - strand at 142.906 kb on + strand at 142.907 kb on - strand at 142.910 kb on + strand at 142.910 kb on + strand at 142.911 kb on - strand at 142.911 kb on - strand at 142.911 kb on - strand at 142.911 kb on - strand at 142.948 kb on - strand at 142.993 kb on + strand at 142.993 kb on + strand at 142.993 kb on + strand at 142.994 kb on - strand at 142.994 kb on - strand at 143.040 kb on - strand at 143.040 kb on - strand at 143.040 kb on - strand at 143.095 kb on + strand at 143.096 kb on - strand at 143.096 kb on - strand at 143.271 kb on + strand, within dcd at 143.272 kb on - strand, within dcd at 143.272 kb on - strand, within dcd at 143.278 kb on - strand, within dcd at 143.434 kb on - strand, within dcd at 143.484 kb on + strand, within dcd at 143.484 kb on + strand, within dcd at 143.485 kb on - strand, within dcd at 143.485 kb on - strand, within dcd at 143.485 kb on - strand, within dcd at 143.485 kb on - strand, within dcd at 143.589 kb on + strand, within dcd at 143.589 kb on + strand, within dcd at 143.589 kb on + strand, within dcd at 143.589 kb on + strand, within dcd at 143.652 kb on + strand, within dcd at 143.652 kb on + strand, within dcd at 143.652 kb on + strand, within dcd at 143.652 kb on + strand, within dcd at 143.653 kb on - strand, within dcd at 143.653 kb on - strand, within dcd at 143.653 kb on - strand, within dcd at 143.653 kb on - strand, within dcd at 143.653 kb on - strand, within dcd at 143.673 kb on + strand at 143.673 kb on + strand at 143.673 kb on + strand at 143.673 kb on + strand at 143.674 kb on - strand at 143.693 kb on + strand at 143.909 kb on + strand, within COO64_RS16935 at 143.909 kb on + strand, within COO64_RS16935 at 143.910 kb on - strand, within COO64_RS16935 at 143.910 kb on - strand, within COO64_RS16935 at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.050 kb on - strand at 144.050 kb on - strand at 144.419 kb on - strand, within COO64_RS16940 at 144.454 kb on - strand, within COO64_RS16940 at 144.462 kb on + strand, within COO64_RS16940 at 144.683 kb on + strand, within COO64_RS16940 at 144.683 kb on + strand, within COO64_RS16940 at 144.683 kb on + strand, within COO64_RS16940 at 144.683 kb on + strand, within COO64_RS16940 at 144.683 kb on + strand, within COO64_RS16940 at 144.684 kb on - strand, within COO64_RS16940 at 144.684 kb on - strand, within COO64_RS16940 at 144.684 kb on - strand, within COO64_RS16940 at 144.684 kb on - strand, within COO64_RS16940
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 142,406 + -0.8 142,530 - +0.7 142,530 - +0.8 142,573 + -1.0 142,573 + -1.6 142,573 + -0.3 142,574 - +0.4 142,585 + +1.0 142,585 + +0.0 142,586 - +0.3 142,586 - -0.4 142,586 - -0.6 142,586 - -0.3 142,589 + -1.1 142,590 - -0.1 142,590 - -1.0 142,590 - +0.5 142,597 - +1.0 142,597 - +0.9 142,597 - +0.4 142,597 - +1.3 142,597 - +0.1 142,597 - +0.4 142,701 + COO64_RS16925 0.56 -0.6 142,701 + COO64_RS16925 0.56 +0.6 142,702 - COO64_RS16925 0.56 -0.3 142,702 - COO64_RS16925 0.56 -0.1 142,702 - COO64_RS16925 0.56 -1.6 142,702 - COO64_RS16925 0.56 +0.0 142,766 + COO64_RS16925 0.87 +0.7 142,766 + COO64_RS16925 0.87 -0.7 142,767 - COO64_RS16925 0.87 +0.3 142,767 - COO64_RS16925 0.87 +0.3 142,767 - COO64_RS16925 0.87 -1.2 142,767 - COO64_RS16925 0.87 -0.9 142,816 + +2.3 142,816 + -0.9 142,816 + +0.7 142,816 + -0.4 142,817 - +0.3 142,820 + +0.6 142,821 - +0.1 142,821 - -0.7 142,821 - +0.3 142,906 + +0.1 142,907 - -1.7 142,910 + -1.7 142,910 + -1.9 142,911 - +0.7 142,911 - -0.6 142,911 - -0.4 142,911 - +0.4 142,948 - +0.5 142,993 + -1.1 142,993 + -0.8 142,993 + +0.7 142,994 - -0.9 142,994 - +0.1 143,040 - +1.3 143,040 - -0.4 143,040 - -2.1 143,095 + -1.1 143,096 - -1.6 143,096 - +0.0 143,271 + dcd COO64_RS16930 0.22 +0.8 143,272 - dcd COO64_RS16930 0.22 +0.4 143,272 - dcd COO64_RS16930 0.22 -0.8 143,278 - dcd COO64_RS16930 0.23 -0.6 143,434 - dcd COO64_RS16930 0.51 -2.0 143,484 + dcd COO64_RS16930 0.60 -1.0 143,484 + dcd COO64_RS16930 0.60 +0.2 143,485 - dcd COO64_RS16930 0.60 -0.4 143,485 - dcd COO64_RS16930 0.60 +0.2 143,485 - dcd COO64_RS16930 0.60 +1.0 143,485 - dcd COO64_RS16930 0.60 +0.7 143,589 + dcd COO64_RS16930 0.78 +0.0 143,589 + dcd COO64_RS16930 0.78 -0.3 143,589 + dcd COO64_RS16930 0.78 -0.3 143,589 + dcd COO64_RS16930 0.78 -0.8 143,652 + dcd COO64_RS16930 0.89 +0.0 143,652 + dcd COO64_RS16930 0.89 -2.0 143,652 + dcd COO64_RS16930 0.89 -0.6 143,652 + dcd COO64_RS16930 0.89 -0.8 143,653 - dcd COO64_RS16930 0.90 +0.4 143,653 - dcd COO64_RS16930 0.90 -0.4 143,653 - dcd COO64_RS16930 0.90 -0.3 143,653 - dcd COO64_RS16930 0.90 +1.5 143,653 - dcd COO64_RS16930 0.90 -2.4 143,673 + +0.6 143,673 + +2.3 143,673 + +1.0 143,673 + +0.0 143,674 - +1.4 143,693 + -0.6 143,909 + COO64_RS16935 0.42 +1.0 143,909 + COO64_RS16935 0.42 +0.3 143,910 - COO64_RS16935 0.42 -0.7 143,910 - COO64_RS16935 0.42 -0.2 144,049 + +1.0 144,049 + -1.0 144,049 + +0.7 144,049 + +1.3 144,049 + +1.8 144,049 + +0.1 144,049 + +0.0 144,050 - -1.6 144,050 - -0.1 144,419 - COO64_RS16940 0.48 +0.1 144,454 - COO64_RS16940 0.53 +0.1 144,462 + COO64_RS16940 0.54 -0.2 144,683 + COO64_RS16940 0.83 +0.0 144,683 + COO64_RS16940 0.83 -0.4 144,683 + COO64_RS16940 0.83 +0.1 144,683 + COO64_RS16940 0.83 +0.2 144,683 + COO64_RS16940 0.83 -1.3 144,684 - COO64_RS16940 0.83 +0.8 144,684 - COO64_RS16940 0.83 -0.5 144,684 - COO64_RS16940 0.83 +1.0 144,684 - COO64_RS16940 0.83 +0.1
Or see this region's nucleotide sequence