Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cmoB and cmoA overlap by 4 nucleotides cmoA and COO64_RS16620 are separated by 85 nucleotides COO64_RS16620 and COO64_RS16625 are separated by 121 nucleotides
COO64_RS16610: cmoB - tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic acid(34) synthase CmoB, at 63,627 to 64,583
cmoB
COO64_RS16615: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 64,580 to 65,323
cmoA
COO64_RS16620: COO64_RS16620 - protease inhibitor I42 family protein, at 65,409 to 65,807
_RS16620
COO64_RS16625: COO64_RS16625 - alpha-xenorhabdolysin family binary toxin subunit A, at 65,929 to 67,092
_RS16625
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-1
0
1
2
3 at 63.724 kb on + strand, within cmoB at 63.997 kb on + strand, within cmoB at 63.998 kb on - strand, within cmoB at 64.097 kb on - strand, within cmoB at 64.189 kb on + strand, within cmoB at 64.189 kb on + strand, within cmoB at 64.190 kb on - strand, within cmoB at 64.190 kb on - strand, within cmoB at 64.190 kb on - strand, within cmoB at 64.190 kb on - strand, within cmoB at 64.190 kb on - strand, within cmoB at 64.425 kb on - strand, within cmoB at 64.610 kb on + strand at 64.610 kb on + strand at 65.019 kb on + strand, within cmoA at 65.019 kb on + strand, within cmoA at 65.019 kb on + strand, within cmoA at 65.020 kb on - strand, within cmoA at 65.020 kb on - strand, within cmoA at 65.020 kb on - strand, within cmoA at 65.020 kb on - strand, within cmoA at 65.139 kb on + strand, within cmoA at 65.140 kb on - strand, within cmoA at 65.140 kb on - strand, within cmoA at 65.140 kb on - strand, within cmoA at 65.301 kb on - strand at 65.365 kb on - strand at 65.410 kb on + strand at 65.410 kb on + strand at 65.476 kb on + strand, within COO64_RS16620 at 65.476 kb on + strand, within COO64_RS16620 at 65.575 kb on + strand, within COO64_RS16620 at 65.575 kb on + strand, within COO64_RS16620 at 65.576 kb on - strand, within COO64_RS16620 at 65.622 kb on + strand, within COO64_RS16620 at 65.623 kb on - strand, within COO64_RS16620 at 65.623 kb on - strand, within COO64_RS16620 at 65.694 kb on + strand, within COO64_RS16620 at 65.694 kb on + strand, within COO64_RS16620 at 65.694 kb on + strand, within COO64_RS16620 at 65.694 kb on + strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.695 kb on - strand, within COO64_RS16620 at 65.832 kb on + strand at 65.882 kb on + strand at 65.882 kb on + strand at 65.882 kb on + strand at 65.882 kb on + strand at 65.882 kb on + strand at 65.883 kb on - strand at 65.883 kb on - strand at 65.883 kb on - strand at 65.914 kb on - strand at 65.914 kb on - strand at 65.914 kb on - strand at 65.937 kb on + strand at 65.937 kb on + strand at 65.937 kb on + strand at 65.938 kb on - strand at 65.938 kb on - strand at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.057 kb on + strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.058 kb on - strand, within COO64_RS16625 at 66.077 kb on - strand, within COO64_RS16625 at 66.085 kb on + strand, within COO64_RS16625 at 66.085 kb on + strand, within COO64_RS16625 at 66.085 kb on + strand, within COO64_RS16625 at 66.085 kb on + strand, within COO64_RS16625 at 66.085 kb on + strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.086 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.088 kb on - strand, within COO64_RS16625 at 66.105 kb on + strand, within COO64_RS16625 at 66.197 kb on + strand, within COO64_RS16625 at 66.198 kb on - strand, within COO64_RS16625 at 66.224 kb on + strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.225 kb on - strand, within COO64_RS16625 at 66.237 kb on + strand, within COO64_RS16625 at 66.237 kb on + strand, within COO64_RS16625 at 66.238 kb on - strand, within COO64_RS16625 at 66.238 kb on - strand, within COO64_RS16625 at 66.238 kb on - strand, within COO64_RS16625 at 66.238 kb on - strand, within COO64_RS16625 at 66.286 kb on - strand, within COO64_RS16625 at 66.302 kb on + strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625 at 66.303 kb on - strand, within COO64_RS16625
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 63,724 + cmoB COO64_RS16610 0.10 -0.4 63,997 + cmoB COO64_RS16610 0.39 -0.1 63,998 - cmoB COO64_RS16610 0.39 -1.2 64,097 - cmoB COO64_RS16610 0.49 +0.9 64,189 + cmoB COO64_RS16610 0.59 -0.4 64,189 + cmoB COO64_RS16610 0.59 +0.6 64,190 - cmoB COO64_RS16610 0.59 +0.1 64,190 - cmoB COO64_RS16610 0.59 -1.2 64,190 - cmoB COO64_RS16610 0.59 +0.2 64,190 - cmoB COO64_RS16610 0.59 +2.4 64,190 - cmoB COO64_RS16610 0.59 -0.3 64,425 - cmoB COO64_RS16610 0.83 +0.8 64,610 + +0.4 64,610 + -0.5 65,019 + cmoA COO64_RS16615 0.59 +0.4 65,019 + cmoA COO64_RS16615 0.59 -0.8 65,019 + cmoA COO64_RS16615 0.59 +0.1 65,020 - cmoA COO64_RS16615 0.59 -1.1 65,020 - cmoA COO64_RS16615 0.59 +0.2 65,020 - cmoA COO64_RS16615 0.59 -0.5 65,020 - cmoA COO64_RS16615 0.59 -0.5 65,139 + cmoA COO64_RS16615 0.75 -0.7 65,140 - cmoA COO64_RS16615 0.75 -0.7 65,140 - cmoA COO64_RS16615 0.75 -0.7 65,140 - cmoA COO64_RS16615 0.75 -0.8 65,301 - -0.8 65,365 - -0.1 65,410 + -0.4 65,410 + -0.3 65,476 + COO64_RS16620 0.17 -0.5 65,476 + COO64_RS16620 0.17 -0.8 65,575 + COO64_RS16620 0.42 -0.8 65,575 + COO64_RS16620 0.42 +0.5 65,576 - COO64_RS16620 0.42 -0.6 65,622 + COO64_RS16620 0.53 +1.0 65,623 - COO64_RS16620 0.54 -0.9 65,623 - COO64_RS16620 0.54 -0.6 65,694 + COO64_RS16620 0.71 -0.1 65,694 + COO64_RS16620 0.71 -0.9 65,694 + COO64_RS16620 0.71 -1.0 65,694 + COO64_RS16620 0.71 -0.5 65,695 - COO64_RS16620 0.72 -0.7 65,695 - COO64_RS16620 0.72 -0.4 65,695 - COO64_RS16620 0.72 +0.0 65,695 - COO64_RS16620 0.72 -0.3 65,695 - COO64_RS16620 0.72 -0.3 65,695 - COO64_RS16620 0.72 +3.3 65,695 - COO64_RS16620 0.72 +0.0 65,695 - COO64_RS16620 0.72 -0.4 65,695 - COO64_RS16620 0.72 +0.1 65,832 + -0.3 65,882 + +0.4 65,882 + +0.5 65,882 + +0.3 65,882 + -0.1 65,882 + +0.0 65,883 - -0.3 65,883 - +0.0 65,883 - -0.4 65,914 - +0.1 65,914 - +0.2 65,914 - +1.0 65,937 + -0.8 65,937 + -1.7 65,937 + -0.6 65,938 - -0.0 65,938 - +0.5 66,057 + COO64_RS16625 0.11 +0.4 66,057 + COO64_RS16625 0.11 -0.4 66,057 + COO64_RS16625 0.11 +0.6 66,057 + COO64_RS16625 0.11 +0.1 66,057 + COO64_RS16625 0.11 +0.4 66,057 + COO64_RS16625 0.11 -0.1 66,057 + COO64_RS16625 0.11 -0.2 66,058 - COO64_RS16625 0.11 -0.1 66,058 - COO64_RS16625 0.11 -0.7 66,058 - COO64_RS16625 0.11 +0.2 66,058 - COO64_RS16625 0.11 -0.6 66,058 - COO64_RS16625 0.11 -1.1 66,058 - COO64_RS16625 0.11 -0.3 66,058 - COO64_RS16625 0.11 -0.5 66,058 - COO64_RS16625 0.11 -1.3 66,058 - COO64_RS16625 0.11 -0.6 66,077 - COO64_RS16625 0.13 -0.0 66,085 + COO64_RS16625 0.13 -0.3 66,085 + COO64_RS16625 0.13 -0.5 66,085 + COO64_RS16625 0.13 -1.5 66,085 + COO64_RS16625 0.13 -1.1 66,085 + COO64_RS16625 0.13 -1.1 66,086 - COO64_RS16625 0.13 -1.1 66,086 - COO64_RS16625 0.13 +1.3 66,086 - COO64_RS16625 0.13 +0.2 66,086 - COO64_RS16625 0.13 -0.7 66,086 - COO64_RS16625 0.13 -0.1 66,086 - COO64_RS16625 0.13 -0.8 66,086 - COO64_RS16625 0.13 +0.7 66,088 - COO64_RS16625 0.14 -0.9 66,088 - COO64_RS16625 0.14 -0.8 66,088 - COO64_RS16625 0.14 +0.1 66,088 - COO64_RS16625 0.14 +0.4 66,088 - COO64_RS16625 0.14 -0.9 66,088 - COO64_RS16625 0.14 +0.2 66,105 + COO64_RS16625 0.15 +0.4 66,197 + COO64_RS16625 0.23 +0.0 66,198 - COO64_RS16625 0.23 -1.0 66,224 + COO64_RS16625 0.25 -0.0 66,225 - COO64_RS16625 0.25 -1.0 66,225 - COO64_RS16625 0.25 -1.0 66,225 - COO64_RS16625 0.25 +0.1 66,225 - COO64_RS16625 0.25 -1.4 66,225 - COO64_RS16625 0.25 -0.1 66,225 - COO64_RS16625 0.25 -1.0 66,225 - COO64_RS16625 0.25 +0.0 66,237 + COO64_RS16625 0.26 +0.9 66,237 + COO64_RS16625 0.26 -0.1 66,238 - COO64_RS16625 0.27 -0.1 66,238 - COO64_RS16625 0.27 +0.5 66,238 - COO64_RS16625 0.27 -0.7 66,238 - COO64_RS16625 0.27 +0.4 66,286 - COO64_RS16625 0.31 -0.7 66,302 + COO64_RS16625 0.32 +1.3 66,303 - COO64_RS16625 0.32 +0.6 66,303 - COO64_RS16625 0.32 -0.4 66,303 - COO64_RS16625 0.32 +1.8 66,303 - COO64_RS16625 0.32 -0.4 66,303 - COO64_RS16625 0.32 -0.0 66,303 - COO64_RS16625 0.32 -0.6
Or see this region's nucleotide sequence