Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rnc and era overlap by 8 nucleotides era and recO are separated by 57 nucleotides recO and pdxJ are separated by 63 nucleotides pdxJ and COO64_RS16575 are separated by 139 nucleotides
COO64_RS16555: rnc - ribonuclease III, at 50,620 to 51,309
rnc
COO64_RS16560: era - GTPase Era, at 51,302 to 52,204
era
COO64_RS16565: recO - DNA repair protein RecO, at 52,262 to 52,945
recO
COO64_RS16570: pdxJ - pyridoxine 5'-phosphate synthase, at 53,009 to 53,734
pdxJ
COO64_RS16575: COO64_RS16575 - heavy metal sensor histidine kinase, at 53,874 to 55,265
_RS16575
Position (kb) 52
53 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 52.289 kb on + strand at 52.289 kb on + strand at 52.290 kb on - strand at 52.290 kb on - strand at 52.290 kb on - strand at 52.290 kb on - strand at 52.310 kb on + strand at 52.311 kb on - strand at 52.526 kb on + strand, within recO at 52.527 kb on - strand, within recO at 52.644 kb on + strand, within recO at 52.644 kb on + strand, within recO at 52.683 kb on - strand, within recO at 52.775 kb on + strand, within recO at 52.775 kb on + strand, within recO at 52.775 kb on + strand, within recO at 52.776 kb on - strand, within recO at 52.776 kb on - strand, within recO at 52.776 kb on - strand, within recO at 52.893 kb on + strand at 52.893 kb on + strand at 52.894 kb on - strand at 52.955 kb on + strand at 52.955 kb on + strand at 52.955 kb on + strand at 52.955 kb on + strand at 52.955 kb on + strand at 52.956 kb on - strand at 52.956 kb on - strand at 52.956 kb on - strand at 52.956 kb on - strand at 52.956 kb on - strand at 52.956 kb on - strand at 52.956 kb on - strand at 53.732 kb on + strand at 53.732 kb on + strand at 53.733 kb on - strand at 53.733 kb on - strand at 53.785 kb on + strand at 53.796 kb on + strand at 53.797 kb on - strand at 53.797 kb on - strand at 53.799 kb on + strand at 53.799 kb on + strand at 53.800 kb on - strand at 53.800 kb on - strand at 53.800 kb on - strand at 53.800 kb on - strand at 53.821 kb on + strand at 53.822 kb on - strand at 53.822 kb on - strand at 53.865 kb on + strand at 53.865 kb on + strand at 53.865 kb on + strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.866 kb on - strand at 53.875 kb on + strand at 53.875 kb on + strand at 53.875 kb on + strand at 53.882 kb on + strand at 53.885 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 52,289 + -0.6 52,289 + -0.1 52,290 - -0.1 52,290 - +1.4 52,290 - -1.3 52,290 - +0.3 52,310 + +0.3 52,311 - -0.7 52,526 + recO COO64_RS16565 0.39 -0.5 52,527 - recO COO64_RS16565 0.39 -2.6 52,644 + recO COO64_RS16565 0.56 +0.0 52,644 + recO COO64_RS16565 0.56 -1.1 52,683 - recO COO64_RS16565 0.62 -0.8 52,775 + recO COO64_RS16565 0.75 -0.8 52,775 + recO COO64_RS16565 0.75 -0.3 52,775 + recO COO64_RS16565 0.75 +0.5 52,776 - recO COO64_RS16565 0.75 +0.8 52,776 - recO COO64_RS16565 0.75 -0.4 52,776 - recO COO64_RS16565 0.75 -0.8 52,893 + +0.2 52,893 + +0.2 52,894 - -1.7 52,955 + -1.3 52,955 + +2.0 52,955 + -0.7 52,955 + +0.8 52,955 + +1.2 52,956 - +0.6 52,956 - -1.6 52,956 - +3.0 52,956 - -0.8 52,956 - +0.2 52,956 - -0.3 52,956 - +0.0 53,732 + +0.4 53,732 + -2.4 53,733 - -0.9 53,733 - -0.1 53,785 + -0.8 53,796 + -1.2 53,797 - +0.5 53,797 - +0.1 53,799 + -0.8 53,799 + +1.5 53,800 - -0.0 53,800 - -0.8 53,800 - -1.2 53,800 - -0.8 53,821 + +0.7 53,822 - -0.3 53,822 - -0.1 53,865 + +0.9 53,865 + +0.2 53,865 + +0.3 53,866 - +0.0 53,866 - +0.0 53,866 - +0.4 53,866 - +1.0 53,866 - -1.0 53,866 - -0.8 53,866 - +0.0 53,866 - -0.3 53,866 - -1.0 53,866 - +1.3 53,875 + -0.5 53,875 + -0.8 53,875 + -0.1 53,882 + +0.8 53,885 + -0.5
Or see this region's nucleotide sequence