Strain Fitness in Pseudomonas sp. SVBP6 around COO64_RS16545

Experiment: supernatant control, M9 with 0.25X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCOO64_RS16540 and lepA are separated by 226 nucleotideslepA and lepB are separated by 5 nucleotides COO64_RS16540: COO64_RS16540 - DegQ family serine endoprotease, at 46,106 to 47,485 _RS16540 COO64_RS16545: lepA - translation elongation factor 4, at 47,712 to 49,511 lepA COO64_RS16550: lepB - signal peptidase I, at 49,517 to 50,371 lepB Position (kb) 47 48 49 50Strain fitness (log2 ratio) -2 -1 0 1at 47.483 kb on + strandat 47.483 kb on + strandat 47.484 kb on - strandat 47.652 kb on + strandat 47.931 kb on + strand, within lepAat 47.931 kb on + strand, within lepAat 47.976 kb on + strand, within lepAat 47.976 kb on + strand, within lepAat 47.976 kb on + strand, within lepAat 48.043 kb on + strand, within lepAat 48.069 kb on + strand, within lepAat 48.069 kb on + strand, within lepAat 48.071 kb on + strand, within lepAat 48.327 kb on + strand, within lepAat 48.327 kb on + strand, within lepAat 48.327 kb on + strand, within lepAat 48.327 kb on + strand, within lepAat 48.327 kb on + strand, within lepAat 48.696 kb on + strand, within lepAat 48.829 kb on + strand, within lepAat 48.829 kb on + strand, within lepAat 48.867 kb on + strand, within lepAat 48.867 kb on + strand, within lepAat 48.867 kb on + strand, within lepAat 48.867 kb on + strand, within lepAat 48.867 kb on + strand, within lepAat 48.977 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.042 kb on + strand, within lepAat 49.047 kb on + strand, within lepAat 49.047 kb on + strand, within lepAat 49.047 kb on + strand, within lepAat 49.113 kb on + strand, within lepAat 49.128 kb on + strand, within lepAat 49.129 kb on - strand, within lepAat 49.174 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.236 kb on + strand, within lepAat 49.337 kb on + strandat 49.380 kb on + strandat 49.505 kb on + strandat 49.505 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 49.516 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.369 kb on + strandat 50.419 kb on + strandat 50.419 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB
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47,483 + -0.5
47,483 + -0.4
47,484 - +0.3
47,652 + +0.0
47,931 + lepA COO64_RS16545 0.12 -0.3
47,931 + lepA COO64_RS16545 0.12 +0.0
47,976 + lepA COO64_RS16545 0.15 -0.3
47,976 + lepA COO64_RS16545 0.15 -0.3
47,976 + lepA COO64_RS16545 0.15 -0.3
48,043 + lepA COO64_RS16545 0.18 -0.6
48,069 + lepA COO64_RS16545 0.20 -1.3
48,069 + lepA COO64_RS16545 0.20 -1.1
48,071 + lepA COO64_RS16545 0.20 +1.0
48,327 + lepA COO64_RS16545 0.34 -0.9
48,327 + lepA COO64_RS16545 0.34 +1.0
48,327 + lepA COO64_RS16545 0.34 -1.4
48,327 + lepA COO64_RS16545 0.34 +0.2
48,327 + lepA COO64_RS16545 0.34 +1.0
48,696 + lepA COO64_RS16545 0.55 -1.0
48,829 + lepA COO64_RS16545 0.62 +0.4
48,829 + lepA COO64_RS16545 0.62 -0.3
48,867 + lepA COO64_RS16545 0.64 -0.2
48,867 + lepA COO64_RS16545 0.64 -1.4
48,867 + lepA COO64_RS16545 0.64 -1.6
48,867 + lepA COO64_RS16545 0.64 -0.2
48,867 + lepA COO64_RS16545 0.64 +0.2
48,977 + lepA COO64_RS16545 0.70 +1.3
49,042 + lepA COO64_RS16545 0.74 -1.6
49,042 + lepA COO64_RS16545 0.74 +0.2
49,042 + lepA COO64_RS16545 0.74 -2.2
49,042 + lepA COO64_RS16545 0.74 -1.7
49,042 + lepA COO64_RS16545 0.74 -1.4
49,042 + lepA COO64_RS16545 0.74 +0.3
49,042 + lepA COO64_RS16545 0.74 -2.1
49,047 + lepA COO64_RS16545 0.74 +0.2
49,047 + lepA COO64_RS16545 0.74 +0.0
49,047 + lepA COO64_RS16545 0.74 -0.8
49,113 + lepA COO64_RS16545 0.78 -0.1
49,128 + lepA COO64_RS16545 0.79 +0.2
49,129 - lepA COO64_RS16545 0.79 -0.3
49,174 + lepA COO64_RS16545 0.81 +1.6
49,236 + lepA COO64_RS16545 0.85 -1.1
49,236 + lepA COO64_RS16545 0.85 +0.8
49,236 + lepA COO64_RS16545 0.85 -0.1
49,236 + lepA COO64_RS16545 0.85 +0.0
49,236 + lepA COO64_RS16545 0.85 -1.1
49,236 + lepA COO64_RS16545 0.85 -0.9
49,236 + lepA COO64_RS16545 0.85 -2.0
49,236 + lepA COO64_RS16545 0.85 -1.7
49,236 + lepA COO64_RS16545 0.85 +0.2
49,337 + -2.1
49,380 + -0.3
49,505 + +0.3
49,505 + +1.3
49,516 + -1.1
49,516 + -0.9
49,516 + +0.5
49,516 + -1.9
49,516 + -0.3
49,516 + +0.8
49,516 + +0.4
50,369 + -1.0
50,369 + +0.2
50,369 + -0.8
50,369 + +0.7
50,369 + -0.9
50,369 + +0.5
50,369 + -1.8
50,419 + -1.8
50,419 + -0.1

Or see this region's nucleotide sequence