Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mrdA and rlmH are separated by 16 nucleotides rlmH and rsfS are separated by 9 nucleotides rsfS and nadD are separated by 45 nucleotides nadD and COO64_RS15630 are separated by 31 nucleotides
COO64_RS15610: mrdA - penicillin-binding protein 2, at 138,907 to 140,796
mrdA
COO64_RS15615: rlmH - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 140,813 to 141,280
rlmH
COO64_RS15620: rsfS - ribosome silencing factor, at 141,290 to 141,718
rsfS
COO64_RS15625: nadD - nicotinate-nucleotide adenylyltransferase, at 141,764 to 142,420
nadD
COO64_RS15630: COO64_RS15630 - glutamate-5-semialdehyde dehydrogenase, at 142,452 to 143,723
_RS15630
Position (kb) 141
142 Strain fitness (log2 ratio) -2
-1
0
1
2 at 140.815 kb on - strand at 140.815 kb on - strand at 140.815 kb on - strand at 140.815 kb on - strand at 140.815 kb on - strand at 140.819 kb on - strand at 140.819 kb on - strand at 140.819 kb on - strand at 140.819 kb on - strand at 140.824 kb on - strand at 140.841 kb on - strand at 140.857 kb on - strand at 140.876 kb on + strand, within rlmH at 140.882 kb on + strand, within rlmH at 140.989 kb on + strand, within rlmH at 141.167 kb on + strand, within rlmH at 141.168 kb on - strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.213 kb on + strand, within rlmH at 141.214 kb on - strand, within rlmH at 141.214 kb on - strand, within rlmH at 141.214 kb on - strand, within rlmH at 141.292 kb on - strand at 141.402 kb on + strand, within rsfS at 141.403 kb on - strand, within rsfS at 141.403 kb on - strand, within rsfS at 141.458 kb on + strand, within rsfS at 141.458 kb on + strand, within rsfS at 141.459 kb on - strand, within rsfS at 141.568 kb on + strand, within rsfS at 141.568 kb on + strand, within rsfS at 141.568 kb on + strand, within rsfS at 141.588 kb on + strand, within rsfS at 141.589 kb on - strand, within rsfS at 141.589 kb on - strand, within rsfS at 141.589 kb on - strand, within rsfS at 141.589 kb on - strand, within rsfS at 141.719 kb on - strand at 141.719 kb on - strand at 141.719 kb on - strand at 141.720 kb on + strand at 141.720 kb on + strand at 141.720 kb on + strand at 141.721 kb on - strand at 141.721 kb on - strand at 141.739 kb on + strand at 141.740 kb on - strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.744 kb on + strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.745 kb on - strand at 141.780 kb on + strand at 141.801 kb on + strand at 141.801 kb on + strand at 141.802 kb on - strand at 141.802 kb on - strand at 141.810 kb on - strand at 141.911 kb on - strand, within nadD at 141.911 kb on - strand, within nadD at 141.911 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.159 kb on - strand, within nadD at 142.439 kb on - strand at 142.498 kb on + strand at 142.498 kb on + strand at 142.499 kb on - strand at 142.499 kb on - strand at 142.499 kb on - strand at 142.499 kb on - strand at 142.499 kb on - strand at 142.499 kb on - strand at 142.616 kb on + strand, within COO64_RS15630 at 142.616 kb on + strand, within COO64_RS15630 at 142.616 kb on + strand, within COO64_RS15630 at 142.616 kb on + strand, within COO64_RS15630 at 142.616 kb on + strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630 at 142.617 kb on - strand, within COO64_RS15630
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 140,815 - -0.8 140,815 - -1.6 140,815 - -1.3 140,815 - -0.1 140,815 - -0.9 140,819 - -0.4 140,819 - +0.6 140,819 - -0.3 140,819 - -0.9 140,824 - +1.5 140,841 - +0.0 140,857 - +0.6 140,876 + rlmH COO64_RS15615 0.13 +0.6 140,882 + rlmH COO64_RS15615 0.15 -0.1 140,989 + rlmH COO64_RS15615 0.38 -0.6 141,167 + rlmH COO64_RS15615 0.76 -0.1 141,168 - rlmH COO64_RS15615 0.76 -0.8 141,213 + rlmH COO64_RS15615 0.85 -2.2 141,213 + rlmH COO64_RS15615 0.85 -0.8 141,213 + rlmH COO64_RS15615 0.85 -0.3 141,213 + rlmH COO64_RS15615 0.85 -0.1 141,213 + rlmH COO64_RS15615 0.85 -1.1 141,213 + rlmH COO64_RS15615 0.85 +1.5 141,214 - rlmH COO64_RS15615 0.86 +0.1 141,214 - rlmH COO64_RS15615 0.86 -0.3 141,214 - rlmH COO64_RS15615 0.86 +1.0 141,292 - +0.3 141,402 + rsfS COO64_RS15620 0.26 -0.3 141,403 - rsfS COO64_RS15620 0.26 -0.1 141,403 - rsfS COO64_RS15620 0.26 +1.2 141,458 + rsfS COO64_RS15620 0.39 -1.4 141,458 + rsfS COO64_RS15620 0.39 +0.0 141,459 - rsfS COO64_RS15620 0.39 -1.0 141,568 + rsfS COO64_RS15620 0.65 -1.3 141,568 + rsfS COO64_RS15620 0.65 -1.8 141,568 + rsfS COO64_RS15620 0.65 -2.4 141,588 + rsfS COO64_RS15620 0.69 -0.1 141,589 - rsfS COO64_RS15620 0.70 -1.2 141,589 - rsfS COO64_RS15620 0.70 -0.3 141,589 - rsfS COO64_RS15620 0.70 -0.6 141,589 - rsfS COO64_RS15620 0.70 +2.0 141,719 - +0.8 141,719 - -1.1 141,719 - +0.4 141,720 + +0.4 141,720 + +0.2 141,720 + -1.4 141,721 - +1.6 141,721 - +1.8 141,739 + -1.9 141,740 - +0.4 141,744 + -0.8 141,744 + +0.0 141,744 + -0.4 141,744 + +0.0 141,744 + -0.4 141,744 + -1.3 141,744 + +0.0 141,744 + -1.0 141,744 + -1.4 141,745 - -0.4 141,745 - -0.8 141,745 - -0.2 141,745 - +0.1 141,745 - +0.5 141,745 - +0.3 141,745 - +0.6 141,745 - +0.5 141,745 - -0.4 141,780 + -0.3 141,801 + -0.4 141,801 + -1.6 141,802 - -0.2 141,802 - +0.9 141,810 - -1.2 141,911 - nadD COO64_RS15625 0.22 -0.1 141,911 - nadD COO64_RS15625 0.22 -0.1 141,911 - nadD COO64_RS15625 0.22 +0.1 142,159 - nadD COO64_RS15625 0.60 +0.6 142,159 - nadD COO64_RS15625 0.60 -0.3 142,159 - nadD COO64_RS15625 0.60 -0.0 142,159 - nadD COO64_RS15625 0.60 +1.0 142,159 - nadD COO64_RS15625 0.60 -0.4 142,159 - nadD COO64_RS15625 0.60 -0.1 142,439 - -1.6 142,498 + +0.4 142,498 + -0.1 142,499 - -0.1 142,499 - -0.7 142,499 - -0.4 142,499 - +0.5 142,499 - +0.4 142,499 - -0.8 142,616 + COO64_RS15630 0.13 -0.3 142,616 + COO64_RS15630 0.13 -0.2 142,616 + COO64_RS15630 0.13 +0.3 142,616 + COO64_RS15630 0.13 +0.3 142,616 + COO64_RS15630 0.13 +0.4 142,617 - COO64_RS15630 0.13 +0.4 142,617 - COO64_RS15630 0.13 -0.4 142,617 - COO64_RS15630 0.13 +0.4 142,617 - COO64_RS15630 0.13 +0.4 142,617 - COO64_RS15630 0.13 -0.1 142,617 - COO64_RS15630 0.13 +0.4
Or see this region's nucleotide sequence