Strain Fitness in Pseudomonas sp. SVBP6 around COO64_RS15550

Experiment: supernatant control, M9 with 0.25X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntleuS and lptE are separated by 66 nucleotideslptE and holA are separated by 36 nucleotides COO64_RS15545: leuS - leucine--tRNA ligase, at 125,295 to 127,901 leuS COO64_RS15550: lptE - LPS assembly lipoprotein LptE, at 127,968 to 128,576 lptE COO64_RS15555: holA - DNA polymerase III subunit delta, at 128,613 to 129,650 holA Position (kb) 127 128 129Strain fitness (log2 ratio) -1 0 1at 127.924 kb on + strandat 127.924 kb on + strandat 127.924 kb on + strandat 127.962 kb on + strandat 127.962 kb on + strandat 127.962 kb on + strandat 127.962 kb on + strandat 127.990 kb on + strandat 128.087 kb on + strand, within lptEat 128.102 kb on + strand, within lptEat 128.166 kb on + strand, within lptEat 128.166 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.214 kb on + strand, within lptEat 128.244 kb on + strand, within lptEat 128.331 kb on + strand, within lptEat 128.331 kb on + strand, within lptEat 128.331 kb on + strand, within lptEat 128.331 kb on + strand, within lptEat 128.331 kb on + strand, within lptEat 128.331 kb on + strand, within lptE

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Per-strain Table

Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB
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127,924 + -0.8
127,924 + +0.6
127,924 + -0.9
127,962 + +1.3
127,962 + -1.6
127,962 + +1.4
127,962 + -0.0
127,990 + -1.3
128,087 + lptE COO64_RS15550 0.20 -1.3
128,102 + lptE COO64_RS15550 0.22 -0.6
128,166 + lptE COO64_RS15550 0.33 -0.6
128,166 + lptE COO64_RS15550 0.33 +0.0
128,214 + lptE COO64_RS15550 0.40 -0.8
128,214 + lptE COO64_RS15550 0.40 -1.3
128,214 + lptE COO64_RS15550 0.40 -0.9
128,214 + lptE COO64_RS15550 0.40 -1.1
128,214 + lptE COO64_RS15550 0.40 -0.6
128,214 + lptE COO64_RS15550 0.40 -1.1
128,214 + lptE COO64_RS15550 0.40 -0.8
128,244 + lptE COO64_RS15550 0.45 +1.0
128,331 + lptE COO64_RS15550 0.60 +0.2
128,331 + lptE COO64_RS15550 0.60 -0.6
128,331 + lptE COO64_RS15550 0.60 -0.6
128,331 + lptE COO64_RS15550 0.60 -0.6
128,331 + lptE COO64_RS15550 0.60 -0.6
128,331 + lptE COO64_RS15550 0.60 -1.1

Or see this region's nucleotide sequence