Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS15290 and COO64_RS15295 overlap by 8 nucleotides COO64_RS15295 and smpB are separated by 161 nucleotides smpB and COO64_RS15305 are separated by 68 nucleotides
COO64_RS15290: COO64_RS15290 - RnfH family protein, at 71,502 to 71,819
_RS15290
COO64_RS15295: COO64_RS15295 - type II toxin-antitoxin system RatA family toxin, at 71,812 to 72,246
_RS15295
COO64_RS15300: smpB - SsrA-binding protein SmpB, at 72,408 to 72,890
smpB
COO64_RS15305: COO64_RS15305 - GntR family transcriptional regulator, at 72,959 to 73,726
_RS15305
Position (kb)
72
73 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 71.431 kb on + strand at 71.431 kb on + strand at 71.431 kb on + strand at 71.431 kb on + strand at 71.432 kb on - strand at 71.432 kb on - strand at 71.461 kb on + strand at 71.471 kb on - strand at 71.618 kb on + strand, within COO64_RS15290 at 71.618 kb on + strand, within COO64_RS15290 at 71.618 kb on + strand, within COO64_RS15290 at 71.618 kb on + strand, within COO64_RS15290 at 71.618 kb on + strand, within COO64_RS15290 at 71.618 kb on + strand, within COO64_RS15290 at 71.619 kb on - strand, within COO64_RS15290 at 71.619 kb on - strand, within COO64_RS15290 at 71.619 kb on - strand, within COO64_RS15290 at 71.729 kb on + strand, within COO64_RS15290 at 71.730 kb on - strand, within COO64_RS15290 at 71.730 kb on - strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.785 kb on + strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.786 kb on - strand, within COO64_RS15290 at 71.802 kb on + strand at 72.363 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.393 kb on + strand at 72.394 kb on - strand at 72.394 kb on - strand at 72.394 kb on - strand at 72.394 kb on - strand at 72.394 kb on - strand at 72.394 kb on - strand at 72.395 kb on + strand at 72.395 kb on + strand at 72.395 kb on + strand at 72.395 kb on + strand at 72.395 kb on + strand at 72.396 kb on - strand at 72.396 kb on - strand at 72.408 kb on - strand at 72.441 kb on - strand at 72.453 kb on - strand at 72.471 kb on + strand, within smpB at 72.472 kb on - strand, within smpB at 72.472 kb on - strand, within smpB at 72.523 kb on + strand, within smpB at 72.523 kb on + strand, within smpB at 72.564 kb on + strand, within smpB at 72.564 kb on + strand, within smpB at 72.564 kb on + strand, within smpB at 72.564 kb on + strand, within smpB at 72.565 kb on - strand, within smpB at 72.565 kb on - strand, within smpB at 72.565 kb on - strand, within smpB at 72.565 kb on - strand, within smpB at 72.565 kb on - strand, within smpB at 72.608 kb on + strand, within smpB at 72.632 kb on + strand, within smpB at 72.632 kb on + strand, within smpB at 72.660 kb on - strand, within smpB at 72.660 kb on - strand, within smpB at 72.727 kb on - strand, within smpB at 72.750 kb on + strand, within smpB at 72.750 kb on + strand, within smpB at 72.750 kb on + strand, within smpB at 72.750 kb on + strand, within smpB at 72.751 kb on - strand, within smpB at 72.751 kb on - strand, within smpB at 72.751 kb on - strand, within smpB at 72.807 kb on + strand, within smpB at 72.807 kb on + strand, within smpB at 72.807 kb on + strand, within smpB at 72.910 kb on + strand at 72.910 kb on + strand at 72.911 kb on - strand at 72.971 kb on - strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.035 kb on + strand at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.036 kb on - strand, within COO64_RS15305 at 73.098 kb on + strand, within COO64_RS15305 at 73.098 kb on + strand, within COO64_RS15305 at 73.099 kb on - strand, within COO64_RS15305 at 73.099 kb on - strand, within COO64_RS15305 at 73.099 kb on - strand, within COO64_RS15305 at 73.152 kb on + strand, within COO64_RS15305 at 73.152 kb on + strand, within COO64_RS15305 at 73.152 kb on + strand, within COO64_RS15305 at 73.152 kb on + strand, within COO64_RS15305 at 73.152 kb on + strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.153 kb on - strand, within COO64_RS15305 at 73.231 kb on + strand, within COO64_RS15305 at 73.232 kb on - strand, within COO64_RS15305 at 73.232 kb on - strand, within COO64_RS15305 at 73.314 kb on + strand, within COO64_RS15305 at 73.314 kb on + strand, within COO64_RS15305 at 73.386 kb on + strand, within COO64_RS15305 at 73.386 kb on + strand, within COO64_RS15305 at 73.386 kb on + strand, within COO64_RS15305 at 73.387 kb on - strand, within COO64_RS15305 at 73.389 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.509 kb on + strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.510 kb on - strand, within COO64_RS15305 at 73.766 kb on + strand at 73.766 kb on + strand at 73.767 kb on - strand at 73.767 kb on - strand at 73.767 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 71,431 + -0.1 71,431 + -0.6 71,431 + +1.4 71,431 + -0.2 71,432 - +0.6 71,432 - +0.8 71,461 + -0.2 71,471 - -0.1 71,618 + COO64_RS15290 0.36 +0.0 71,618 + COO64_RS15290 0.36 -0.3 71,618 + COO64_RS15290 0.36 -0.7 71,618 + COO64_RS15290 0.36 +0.7 71,618 + COO64_RS15290 0.36 -1.4 71,618 + COO64_RS15290 0.36 +1.1 71,619 - COO64_RS15290 0.37 -0.5 71,619 - COO64_RS15290 0.37 -0.0 71,619 - COO64_RS15290 0.37 +0.1 71,729 + COO64_RS15290 0.71 +0.0 71,730 - COO64_RS15290 0.72 -0.7 71,730 - COO64_RS15290 0.72 +0.0 71,785 + COO64_RS15290 0.89 +0.3 71,785 + COO64_RS15290 0.89 +0.4 71,785 + COO64_RS15290 0.89 +1.0 71,785 + COO64_RS15290 0.89 +0.2 71,785 + COO64_RS15290 0.89 +0.3 71,785 + COO64_RS15290 0.89 +0.0 71,785 + COO64_RS15290 0.89 +0.5 71,786 - COO64_RS15290 0.89 -0.6 71,786 - COO64_RS15290 0.89 -1.4 71,786 - COO64_RS15290 0.89 +0.9 71,786 - COO64_RS15290 0.89 -0.0 71,786 - COO64_RS15290 0.89 -0.5 71,786 - COO64_RS15290 0.89 -0.2 71,786 - COO64_RS15290 0.89 +0.3 71,786 - COO64_RS15290 0.89 -0.8 71,786 - COO64_RS15290 0.89 -0.4 71,786 - COO64_RS15290 0.89 -0.4 71,786 - COO64_RS15290 0.89 -0.1 71,786 - COO64_RS15290 0.89 +0.4 71,786 - COO64_RS15290 0.89 -0.2 71,786 - COO64_RS15290 0.89 -0.0 71,786 - COO64_RS15290 0.89 +0.2 71,786 - COO64_RS15290 0.89 +0.3 71,802 + -0.9 72,363 + +0.3 72,393 + +0.7 72,393 + +0.7 72,393 + +0.7 72,393 + +1.0 72,393 + -0.3 72,393 + -0.2 72,393 + +1.7 72,393 + -0.1 72,393 + -0.4 72,394 - +1.3 72,394 - +1.4 72,394 - -0.7 72,394 - -0.6 72,394 - -1.0 72,394 - -1.7 72,395 + +1.0 72,395 + -0.1 72,395 + -0.1 72,395 + -0.6 72,395 + -1.6 72,396 - +0.0 72,396 - +0.7 72,408 - -1.3 72,441 - -0.4 72,453 - -2.6 72,471 + smpB COO64_RS15300 0.13 +0.4 72,472 - smpB COO64_RS15300 0.13 +0.4 72,472 - smpB COO64_RS15300 0.13 +0.8 72,523 + smpB COO64_RS15300 0.24 +1.2 72,523 + smpB COO64_RS15300 0.24 -0.3 72,564 + smpB COO64_RS15300 0.32 +2.0 72,564 + smpB COO64_RS15300 0.32 -0.3 72,564 + smpB COO64_RS15300 0.32 -0.3 72,564 + smpB COO64_RS15300 0.32 -0.9 72,565 - smpB COO64_RS15300 0.33 +0.8 72,565 - smpB COO64_RS15300 0.33 -0.8 72,565 - smpB COO64_RS15300 0.33 +0.2 72,565 - smpB COO64_RS15300 0.33 -1.8 72,565 - smpB COO64_RS15300 0.33 -1.4 72,608 + smpB COO64_RS15300 0.41 -0.9 72,632 + smpB COO64_RS15300 0.46 -0.4 72,632 + smpB COO64_RS15300 0.46 +1.0 72,660 - smpB COO64_RS15300 0.52 -0.8 72,660 - smpB COO64_RS15300 0.52 -1.3 72,727 - smpB COO64_RS15300 0.66 -1.0 72,750 + smpB COO64_RS15300 0.71 -0.1 72,750 + smpB COO64_RS15300 0.71 -1.0 72,750 + smpB COO64_RS15300 0.71 -1.2 72,750 + smpB COO64_RS15300 0.71 -0.1 72,751 - smpB COO64_RS15300 0.71 +1.0 72,751 - smpB COO64_RS15300 0.71 -0.3 72,751 - smpB COO64_RS15300 0.71 -1.0 72,807 + smpB COO64_RS15300 0.83 +0.2 72,807 + smpB COO64_RS15300 0.83 +0.4 72,807 + smpB COO64_RS15300 0.83 +0.7 72,910 + -0.4 72,910 + -0.2 72,911 - -0.1 72,971 - +0.9 73,035 + -0.2 73,035 + +0.4 73,035 + -0.1 73,035 + -0.5 73,035 + +0.3 73,035 + +0.7 73,035 + +0.2 73,035 + +0.1 73,035 + -1.0 73,035 + +1.0 73,035 + +0.7 73,036 - COO64_RS15305 0.10 +1.4 73,036 - COO64_RS15305 0.10 -0.2 73,036 - COO64_RS15305 0.10 -0.2 73,036 - COO64_RS15305 0.10 +1.1 73,036 - COO64_RS15305 0.10 +0.6 73,036 - COO64_RS15305 0.10 -0.4 73,036 - COO64_RS15305 0.10 +0.4 73,036 - COO64_RS15305 0.10 -0.2 73,036 - COO64_RS15305 0.10 -0.5 73,036 - COO64_RS15305 0.10 +0.7 73,036 - COO64_RS15305 0.10 -0.3 73,036 - COO64_RS15305 0.10 -0.1 73,036 - COO64_RS15305 0.10 +0.1 73,036 - COO64_RS15305 0.10 +0.3 73,036 - COO64_RS15305 0.10 -0.3 73,036 - COO64_RS15305 0.10 +0.1 73,036 - COO64_RS15305 0.10 -1.6 73,036 - COO64_RS15305 0.10 +0.4 73,036 - COO64_RS15305 0.10 +2.0 73,098 + COO64_RS15305 0.18 -0.6 73,098 + COO64_RS15305 0.18 +0.5 73,099 - COO64_RS15305 0.18 +0.3 73,099 - COO64_RS15305 0.18 -1.7 73,099 - COO64_RS15305 0.18 +0.1 73,152 + COO64_RS15305 0.25 +0.6 73,152 + COO64_RS15305 0.25 -0.6 73,152 + COO64_RS15305 0.25 +0.1 73,152 + COO64_RS15305 0.25 -0.8 73,152 + COO64_RS15305 0.25 -0.7 73,153 - COO64_RS15305 0.25 -1.6 73,153 - COO64_RS15305 0.25 -0.9 73,153 - COO64_RS15305 0.25 +0.4 73,153 - COO64_RS15305 0.25 -0.2 73,153 - COO64_RS15305 0.25 -1.1 73,153 - COO64_RS15305 0.25 +0.0 73,153 - COO64_RS15305 0.25 -1.8 73,153 - COO64_RS15305 0.25 -0.0 73,153 - COO64_RS15305 0.25 +1.2 73,153 - COO64_RS15305 0.25 -0.2 73,231 + COO64_RS15305 0.35 +0.2 73,232 - COO64_RS15305 0.36 -0.8 73,232 - COO64_RS15305 0.36 +0.3 73,314 + COO64_RS15305 0.46 -1.6 73,314 + COO64_RS15305 0.46 -0.2 73,386 + COO64_RS15305 0.56 -0.6 73,386 + COO64_RS15305 0.56 -1.3 73,386 + COO64_RS15305 0.56 +0.5 73,387 - COO64_RS15305 0.56 +0.8 73,389 + COO64_RS15305 0.56 -0.1 73,509 + COO64_RS15305 0.72 -1.1 73,509 + COO64_RS15305 0.72 +0.7 73,509 + COO64_RS15305 0.72 +0.9 73,509 + COO64_RS15305 0.72 -0.1 73,509 + COO64_RS15305 0.72 -0.8 73,509 + COO64_RS15305 0.72 -0.2 73,509 + COO64_RS15305 0.72 -0.4 73,509 + COO64_RS15305 0.72 +1.0 73,509 + COO64_RS15305 0.72 -0.3 73,509 + COO64_RS15305 0.72 +1.2 73,509 + COO64_RS15305 0.72 +0.4 73,509 + COO64_RS15305 0.72 -0.6 73,509 + COO64_RS15305 0.72 +0.5 73,509 + COO64_RS15305 0.72 -0.1 73,509 + COO64_RS15305 0.72 -0.2 73,509 + COO64_RS15305 0.72 +0.6 73,509 + COO64_RS15305 0.72 +0.0 73,509 + COO64_RS15305 0.72 -0.7 73,509 + COO64_RS15305 0.72 +0.4 73,509 + COO64_RS15305 0.72 -1.1 73,509 + COO64_RS15305 0.72 -0.7 73,509 + COO64_RS15305 0.72 +1.6 73,509 + COO64_RS15305 0.72 +0.4 73,510 - COO64_RS15305 0.72 -0.5 73,510 - COO64_RS15305 0.72 +0.2 73,510 - COO64_RS15305 0.72 -0.8 73,510 - COO64_RS15305 0.72 -0.4 73,510 - COO64_RS15305 0.72 +0.0 73,510 - COO64_RS15305 0.72 -0.4 73,510 - COO64_RS15305 0.72 +0.5 73,510 - COO64_RS15305 0.72 +0.4 73,510 - COO64_RS15305 0.72 +0.9 73,510 - COO64_RS15305 0.72 +0.0 73,510 - COO64_RS15305 0.72 +0.3 73,510 - COO64_RS15305 0.72 -0.1 73,510 - COO64_RS15305 0.72 +0.0 73,510 - COO64_RS15305 0.72 +0.9 73,510 - COO64_RS15305 0.72 +0.7 73,510 - COO64_RS15305 0.72 -0.1 73,510 - COO64_RS15305 0.72 +0.3 73,510 - COO64_RS15305 0.72 -0.6 73,510 - COO64_RS15305 0.72 +0.6 73,510 - COO64_RS15305 0.72 +0.1 73,510 - COO64_RS15305 0.72 +0.6 73,510 - COO64_RS15305 0.72 -0.4 73,766 + +0.7 73,766 + -1.9 73,767 - +0.1 73,767 - -0.8 73,767 - +0.1
Or see this region's nucleotide sequence