Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmE and yhbY are separated by 100 nucleotides yhbY and COO64_RS15230 are separated by 12 nucleotides COO64_RS15230 and greA overlap by 23 nucleotides greA and carB overlap by 4 nucleotides
COO64_RS15220: rlmE - 23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE, at 54,244 to 54,867
rlmE
COO64_RS15225: yhbY - ribosome assembly RNA-binding protein YhbY, at 54,968 to 55,276
yhbY
COO64_RS15230: COO64_RS15230 - MFS transporter, at 55,289 to 55,735
_RS15230
COO64_RS15235: greA - transcription elongation factor GreA, at 55,713 to 56,195
greA
COO64_RS15240: carB - carbamoyl-phosphate synthase large subunit, at 56,192 to 59,413
carB
Position (kb) 55
56
57 Strain fitness (log2 ratio) -2
-1
0
1 at 54.884 kb on - strand at 54.884 kb on - strand at 54.884 kb on - strand at 54.884 kb on - strand at 54.884 kb on - strand at 54.884 kb on - strand at 54.884 kb on - strand at 54.897 kb on - strand at 54.897 kb on - strand at 54.968 kb on - strand at 54.980 kb on - strand at 54.999 kb on - strand, within yhbY at 54.999 kb on - strand, within yhbY at 54.999 kb on - strand, within yhbY at 55.192 kb on - strand, within yhbY at 55.192 kb on - strand, within yhbY at 55.221 kb on - strand, within yhbY at 55.268 kb on + strand at 55.269 kb on - strand at 55.365 kb on + strand, within COO64_RS15230 at 55.366 kb on - strand, within COO64_RS15230 at 55.419 kb on + strand, within COO64_RS15230 at 55.420 kb on - strand, within COO64_RS15230 at 55.876 kb on + strand, within greA at 55.876 kb on + strand, within greA at 55.876 kb on + strand, within greA at 55.877 kb on - strand, within greA at 56.007 kb on - strand, within greA at 56.047 kb on + strand, within greA at 56.047 kb on + strand, within greA at 56.301 kb on + strand at 56.301 kb on + strand at 56.302 kb on - strand at 56.302 kb on - strand at 56.302 kb on - strand at 56.302 kb on - strand at 56.337 kb on + strand at 56.755 kb on - strand, within carB at 56.761 kb on + strand, within carB at 56.762 kb on - strand, within carB at 56.904 kb on + strand, within carB at 56.904 kb on + strand, within carB at 56.904 kb on + strand, within carB at 56.904 kb on + strand, within carB at 56.904 kb on + strand, within carB at 56.905 kb on - strand, within carB at 56.905 kb on - strand, within carB at 56.905 kb on - strand, within carB
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 54,884 - +1.0 54,884 - +0.1 54,884 - -0.6 54,884 - +1.1 54,884 - -0.3 54,884 - +0.0 54,884 - -0.7 54,897 - -0.1 54,897 - +0.3 54,968 - +0.6 54,980 - +0.3 54,999 - yhbY COO64_RS15225 0.10 +0.8 54,999 - yhbY COO64_RS15225 0.10 +0.0 54,999 - yhbY COO64_RS15225 0.10 +0.1 55,192 - yhbY COO64_RS15225 0.72 +0.0 55,192 - yhbY COO64_RS15225 0.72 -0.9 55,221 - yhbY COO64_RS15225 0.82 -0.4 55,268 + -0.8 55,269 - +1.2 55,365 + COO64_RS15230 0.17 -1.1 55,366 - COO64_RS15230 0.17 +0.5 55,419 + COO64_RS15230 0.29 +0.6 55,420 - COO64_RS15230 0.29 -0.9 55,876 + greA COO64_RS15235 0.34 -1.5 55,876 + greA COO64_RS15235 0.34 -0.6 55,876 + greA COO64_RS15235 0.34 -1.6 55,877 - greA COO64_RS15235 0.34 -0.3 56,007 - greA COO64_RS15235 0.61 -1.7 56,047 + greA COO64_RS15235 0.69 +0.4 56,047 + greA COO64_RS15235 0.69 -1.0 56,301 + -1.0 56,301 + +0.4 56,302 - -0.8 56,302 - -1.3 56,302 - -1.6 56,302 - -0.3 56,337 + -1.1 56,755 - carB COO64_RS15240 0.17 -0.8 56,761 + carB COO64_RS15240 0.18 -0.3 56,762 - carB COO64_RS15240 0.18 +1.0 56,904 + carB COO64_RS15240 0.22 -0.1 56,904 + carB COO64_RS15240 0.22 -2.1 56,904 + carB COO64_RS15240 0.22 -1.7 56,904 + carB COO64_RS15240 0.22 +1.2 56,904 + carB COO64_RS15240 0.22 -1.7 56,905 - carB COO64_RS15240 0.22 -0.7 56,905 - carB COO64_RS15240 0.22 -0.3 56,905 - carB COO64_RS15240 0.22 +0.0
Or see this region's nucleotide sequence