Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nusA and rimP are separated by 59 nucleotides rimP and COO64_RS15185 are separated by 129 nucleotides COO64_RS15185 and COO64_RS15190 are separated by 97 nucleotides COO64_RS15190 and secG are separated by 19 nucleotides secG and tpiA are separated by 3 nucleotides tpiA and glmM are separated by 66 nucleotides
COO64_RS15175: nusA - transcription termination factor NusA, at 46,287 to 47,768
nusA
COO64_RS15180: rimP - ribosome maturation factor RimP, at 47,828 to 48,286
rimP
COO64_RS15185: COO64_RS15185 - tRNA-Met, at 48,416 to 48,492
_RS15185
COO64_RS15190: COO64_RS15190 - tRNA-Leu, at 48,590 to 48,675
_RS15190
COO64_RS15195: secG - preprotein translocase subunit SecG, at 48,695 to 49,081
secG
COO64_RS15200: tpiA - triose-phosphate isomerase, at 49,085 to 49,840
tpiA
COO64_RS15205: glmM - phosphoglucosamine mutase, at 49,907 to 51,247
glmM
Position (kb) 48
49
50 Strain fitness (log2 ratio) -1
0
1 at 47.846 kb on - strand at 48.308 kb on - strand at 48.308 kb on - strand at 48.308 kb on - strand at 48.366 kb on - strand at 48.551 kb on + strand at 48.552 kb on - strand at 48.679 kb on - strand at 48.682 kb on - strand at 48.682 kb on - strand at 48.682 kb on - strand at 48.697 kb on - strand at 48.697 kb on - strand at 48.734 kb on - strand, within secG at 48.768 kb on - strand, within secG at 48.768 kb on - strand, within secG at 48.768 kb on - strand, within secG at 48.768 kb on - strand, within secG at 48.768 kb on - strand, within secG at 48.828 kb on - strand, within secG at 48.828 kb on - strand, within secG at 48.828 kb on - strand, within secG at 48.828 kb on - strand, within secG at 48.828 kb on - strand, within secG at 48.865 kb on - strand, within secG at 48.865 kb on - strand, within secG at 48.865 kb on - strand, within secG at 49.760 kb on - strand, within tpiA at 49.846 kb on - strand at 49.846 kb on - strand at 49.903 kb on + strand at 49.904 kb on - strand at 49.904 kb on - strand at 49.926 kb on - strand at 49.926 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 47,846 - +0.4 48,308 - +0.4 48,308 - -0.9 48,308 - -0.6 48,366 - -0.2 48,551 + +0.1 48,552 - -1.1 48,679 - -0.5 48,682 - -0.5 48,682 - -0.8 48,682 - +0.1 48,697 - +0.2 48,697 - +0.2 48,734 - secG COO64_RS15195 0.10 -0.8 48,768 - secG COO64_RS15195 0.19 +0.0 48,768 - secG COO64_RS15195 0.19 +0.5 48,768 - secG COO64_RS15195 0.19 -0.2 48,768 - secG COO64_RS15195 0.19 +0.0 48,768 - secG COO64_RS15195 0.19 -0.8 48,828 - secG COO64_RS15195 0.34 -1.0 48,828 - secG COO64_RS15195 0.34 -1.4 48,828 - secG COO64_RS15195 0.34 -0.8 48,828 - secG COO64_RS15195 0.34 -1.0 48,828 - secG COO64_RS15195 0.34 +1.0 48,865 - secG COO64_RS15195 0.44 +0.3 48,865 - secG COO64_RS15195 0.44 -0.6 48,865 - secG COO64_RS15195 0.44 +0.8 49,760 - tpiA COO64_RS15200 0.89 -0.6 49,846 - +0.6 49,846 - -0.6 49,903 + +0.2 49,904 - +0.4 49,904 - -0.5 49,926 - -0.7 49,926 - -0.1
Or see this region's nucleotide sequence