Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS14630 and COO64_RS14635 are separated by 11 nucleotides COO64_RS14635 and cobW are separated by 680 nucleotides cobW and cobN are separated by 3 nucleotides
COO64_RS14630: COO64_RS14630 - CbtA family protein, at 491,654 to 492,346
_RS14630
COO64_RS14635: COO64_RS14635 - CbtB domain-containing protein, at 492,358 to 492,543
_RS14635
COO64_RS14640: cobW - cobalamin biosynthesis protein CobW, at 493,224 to 494,291
cobW
COO64_RS14645: cobN - cobaltochelatase subunit CobN, at 494,295 to 498,056
cobN
Position (kb) 493
494
495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 492.234 kb on + strand, within COO64_RS14630 at 492.234 kb on + strand, within COO64_RS14630 at 492.234 kb on + strand, within COO64_RS14630 at 492.234 kb on + strand, within COO64_RS14630 at 492.235 kb on - strand, within COO64_RS14630 at 492.235 kb on - strand, within COO64_RS14630 at 492.334 kb on + strand at 492.334 kb on + strand at 492.334 kb on + strand at 492.335 kb on - strand at 492.440 kb on + strand, within COO64_RS14635 at 492.440 kb on + strand, within COO64_RS14635 at 492.441 kb on - strand, within COO64_RS14635 at 492.441 kb on - strand, within COO64_RS14635 at 492.522 kb on + strand, within COO64_RS14635 at 492.523 kb on - strand, within COO64_RS14635 at 492.538 kb on - strand at 492.538 kb on - strand at 492.761 kb on - strand at 492.761 kb on - strand at 492.761 kb on - strand at 492.811 kb on + strand at 492.811 kb on + strand at 492.811 kb on + strand at 492.811 kb on + strand at 492.812 kb on - strand at 492.812 kb on - strand at 492.812 kb on - strand at 492.812 kb on - strand at 492.812 kb on - strand at 492.867 kb on - strand at 492.867 kb on - strand at 492.931 kb on + strand at 492.932 kb on - strand at 492.936 kb on + strand at 492.972 kb on - strand at 493.014 kb on + strand at 493.015 kb on - strand at 493.015 kb on - strand at 493.015 kb on - strand at 493.015 kb on - strand at 493.301 kb on + strand at 493.301 kb on + strand at 493.301 kb on + strand at 493.301 kb on + strand at 493.302 kb on - strand at 493.302 kb on - strand at 493.431 kb on + strand, within cobW at 493.431 kb on + strand, within cobW at 493.431 kb on + strand, within cobW at 493.431 kb on + strand, within cobW at 493.431 kb on + strand, within cobW at 493.526 kb on + strand, within cobW at 493.526 kb on + strand, within cobW at 493.526 kb on + strand, within cobW at 493.527 kb on - strand, within cobW at 494.329 kb on + strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.343 kb on - strand at 494.377 kb on + strand at 494.377 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.505 kb on + strand at 494.506 kb on - strand at 494.506 kb on - strand at 494.506 kb on - strand at 494.506 kb on - strand at 494.506 kb on - strand at 494.506 kb on - strand at 494.578 kb on - strand at 494.829 kb on + strand, within cobN at 494.829 kb on + strand, within cobN at 494.830 kb on - strand, within cobN at 494.830 kb on - strand, within cobN at 494.830 kb on - strand, within cobN at 494.830 kb on - strand, within cobN at 494.830 kb on - strand, within cobN at 494.914 kb on - strand, within cobN at 494.955 kb on - strand, within cobN
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 492,234 + COO64_RS14630 0.84 -1.6 492,234 + COO64_RS14630 0.84 -1.4 492,234 + COO64_RS14630 0.84 +1.5 492,234 + COO64_RS14630 0.84 -1.0 492,235 - COO64_RS14630 0.84 +0.0 492,235 - COO64_RS14630 0.84 -1.9 492,334 + +0.4 492,334 + +1.8 492,334 + +1.5 492,335 - -0.1 492,440 + COO64_RS14635 0.44 -0.7 492,440 + COO64_RS14635 0.44 -0.7 492,441 - COO64_RS14635 0.45 +0.8 492,441 - COO64_RS14635 0.45 +0.7 492,522 + COO64_RS14635 0.88 +1.1 492,523 - COO64_RS14635 0.89 -1.6 492,538 - -1.3 492,538 - +1.8 492,761 - -0.3 492,761 - +1.0 492,761 - -1.7 492,811 + -0.0 492,811 + -0.1 492,811 + -1.8 492,811 + +0.6 492,812 - -0.2 492,812 - -0.4 492,812 - -1.0 492,812 - -0.2 492,812 - +1.8 492,867 - +0.5 492,867 - -0.1 492,931 + -0.5 492,932 - -1.3 492,936 + -0.1 492,972 - +0.2 493,014 + -0.2 493,015 - +0.5 493,015 - +0.1 493,015 - -1.6 493,015 - +0.8 493,301 + -2.8 493,301 + +0.8 493,301 + +0.3 493,301 + +0.3 493,302 - -2.8 493,302 - +0.7 493,431 + cobW COO64_RS14640 0.19 -0.6 493,431 + cobW COO64_RS14640 0.19 +0.4 493,431 + cobW COO64_RS14640 0.19 -2.2 493,431 + cobW COO64_RS14640 0.19 -1.3 493,431 + cobW COO64_RS14640 0.19 -1.8 493,526 + cobW COO64_RS14640 0.28 -1.1 493,526 + cobW COO64_RS14640 0.28 +0.2 493,526 + cobW COO64_RS14640 0.28 -0.3 493,527 - cobW COO64_RS14640 0.28 +0.3 494,329 + +0.5 494,343 - +0.2 494,343 - +1.6 494,343 - +0.3 494,377 + -0.7 494,377 + -0.4 494,505 + -0.3 494,505 + +0.6 494,505 + +0.1 494,505 + +0.8 494,505 + +4.4 494,505 + -2.0 494,505 + +1.5 494,505 + -1.4 494,505 + +0.3 494,505 + +0.5 494,506 - +0.0 494,506 - -0.3 494,506 - -0.6 494,506 - +0.0 494,506 - +0.4 494,506 - +0.2 494,578 - -0.8 494,829 + cobN COO64_RS14645 0.14 -0.8 494,829 + cobN COO64_RS14645 0.14 -1.0 494,830 - cobN COO64_RS14645 0.14 -0.9 494,830 - cobN COO64_RS14645 0.14 -0.2 494,830 - cobN COO64_RS14645 0.14 +0.5 494,830 - cobN COO64_RS14645 0.14 +0.2 494,830 - cobN COO64_RS14645 0.14 +1.2 494,914 - cobN COO64_RS14645 0.16 -0.7 494,955 - cobN COO64_RS14645 0.18 -0.4
Or see this region's nucleotide sequence