Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS13570 and COO64_RS13575 overlap by 4 nucleotides COO64_RS13575 and pyrR are separated by 12 nucleotides pyrR and ruvX are separated by 62 nucleotides
COO64_RS13570: COO64_RS13570 - dihydroorotase, at 266,394 to 267,665
_RS13570
COO64_RS13575: COO64_RS13575 - aspartate carbamoyltransferase catalytic subunit, at 267,662 to 268,666
_RS13575
COO64_RS13580: pyrR - bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR, at 268,679 to 269,197
pyrR
COO64_RS13585: ruvX - Holliday junction resolvase RuvX, at 269,260 to 269,691
ruvX
Position (kb) 267
268
269 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 266.839 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.888 kb on - strand, within COO64_RS13570 at 267.160 kb on + strand, within COO64_RS13570 at 267.161 kb on - strand, within COO64_RS13570 at 267.223 kb on - strand, within COO64_RS13570 at 267.301 kb on + strand, within COO64_RS13570 at 267.301 kb on + strand, within COO64_RS13570 at 267.369 kb on + strand, within COO64_RS13570 at 267.653 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.897 kb on + strand, within COO64_RS13575 at 267.897 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.017 kb on + strand, within COO64_RS13575 at 268.017 kb on + strand, within COO64_RS13575 at 268.018 kb on - strand, within COO64_RS13575 at 268.681 kb on - strand at 268.681 kb on - strand at 268.860 kb on + strand, within pyrR at 268.861 kb on - strand, within pyrR at 269.010 kb on + strand, within pyrR at 269.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 266,839 + COO64_RS13570 0.35 -1.2 266,887 + COO64_RS13570 0.39 +0.8 266,887 + COO64_RS13570 0.39 -0.8 266,887 + COO64_RS13570 0.39 -0.7 266,887 + COO64_RS13570 0.39 +0.4 266,888 - COO64_RS13570 0.39 -0.6 267,160 + COO64_RS13570 0.60 -3.0 267,161 - COO64_RS13570 0.60 -0.6 267,223 - COO64_RS13570 0.65 -1.9 267,301 + COO64_RS13570 0.71 -0.5 267,301 + COO64_RS13570 0.71 -1.6 267,369 + COO64_RS13570 0.77 -2.4 267,653 + -0.5 267,750 + -1.9 267,750 + -1.4 267,750 + -2.0 267,750 + -3.7 267,750 + -2.6 267,750 + -1.9 267,751 - -2.0 267,751 - -2.9 267,751 - -1.1 267,751 - -1.6 267,897 + COO64_RS13575 0.23 -0.7 267,897 + COO64_RS13575 0.23 -1.6 268,002 + COO64_RS13575 0.34 -2.7 268,002 + COO64_RS13575 0.34 -0.5 268,002 + COO64_RS13575 0.34 -1.2 268,003 - COO64_RS13575 0.34 -3.5 268,003 - COO64_RS13575 0.34 -2.2 268,003 - COO64_RS13575 0.34 -2.8 268,017 + COO64_RS13575 0.35 -1.9 268,017 + COO64_RS13575 0.35 -0.8 268,018 - COO64_RS13575 0.35 -1.4 268,681 - -2.1 268,681 - -2.1 268,860 + pyrR COO64_RS13580 0.35 +0.1 268,861 - pyrR COO64_RS13580 0.35 -0.8 269,010 + pyrR COO64_RS13580 0.64 +0.1 269,190 - +0.8
Or see this region's nucleotide sequence