Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hslU and hslV are separated by 77 nucleotides hslV and COO64_RS13570 are separated by 184 nucleotides COO64_RS13570 and COO64_RS13575 overlap by 4 nucleotides
COO64_RS13560: hslU - ATP-dependent protease ATPase subunit HslU, at 264,264 to 265,601
hslU
COO64_RS13565: hslV - ATP-dependent protease subunit HslV, at 265,679 to 266,209
hslV
COO64_RS13570: COO64_RS13570 - dihydroorotase, at 266,394 to 267,665
_RS13570
COO64_RS13575: COO64_RS13575 - aspartate carbamoyltransferase catalytic subunit, at 267,662 to 268,666
_RS13575
Position (kb) 266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.605 kb on - strand at 265.626 kb on + strand at 265.627 kb on - strand at 265.627 kb on - strand at 265.824 kb on + strand, within hslV at 266.095 kb on - strand, within hslV at 266.256 kb on + strand at 266.256 kb on + strand at 266.256 kb on + strand at 266.256 kb on + strand at 266.257 kb on - strand at 266.257 kb on - strand at 266.283 kb on + strand at 266.283 kb on + strand at 266.283 kb on + strand at 266.283 kb on + strand at 266.283 kb on + strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.284 kb on - strand at 266.304 kb on + strand at 266.305 kb on - strand at 266.363 kb on + strand at 266.364 kb on - strand at 266.364 kb on - strand at 266.364 kb on - strand at 266.411 kb on + strand at 266.412 kb on - strand at 266.412 kb on - strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.429 kb on + strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.430 kb on - strand at 266.431 kb on + strand at 266.431 kb on + strand at 266.432 kb on - strand at 266.839 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.887 kb on + strand, within COO64_RS13570 at 266.888 kb on - strand, within COO64_RS13570 at 267.160 kb on + strand, within COO64_RS13570 at 267.161 kb on - strand, within COO64_RS13570 at 267.223 kb on - strand, within COO64_RS13570 at 267.301 kb on + strand, within COO64_RS13570 at 267.301 kb on + strand, within COO64_RS13570 at 267.369 kb on + strand, within COO64_RS13570 at 267.653 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.750 kb on + strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.751 kb on - strand at 267.897 kb on + strand, within COO64_RS13575 at 267.897 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.002 kb on + strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.003 kb on - strand, within COO64_RS13575 at 268.017 kb on + strand, within COO64_RS13575 at 268.017 kb on + strand, within COO64_RS13575 at 268.018 kb on - strand, within COO64_RS13575
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 265,605 - +0.6 265,605 - -0.3 265,605 - +0.5 265,605 - -0.3 265,605 - -0.5 265,605 - -1.4 265,605 - +0.4 265,605 - +0.4 265,605 - +0.2 265,626 + -0.2 265,627 - +0.6 265,627 - -0.6 265,824 + hslV COO64_RS13565 0.27 +0.6 266,095 - hslV COO64_RS13565 0.78 +0.8 266,256 + -0.2 266,256 + -0.4 266,256 + +0.7 266,256 + +0.5 266,257 - +0.6 266,257 - +0.2 266,283 + -0.2 266,283 + +0.4 266,283 + +0.1 266,283 + -0.7 266,283 + +1.0 266,284 - +0.4 266,284 - +1.0 266,284 - +0.3 266,284 - -0.4 266,284 - +1.5 266,284 - -1.2 266,284 - -0.6 266,284 - +0.7 266,284 - -0.6 266,304 + -0.0 266,305 - -3.2 266,363 + +0.5 266,364 - -0.4 266,364 - -0.8 266,364 - +0.5 266,411 + +0.1 266,412 - -1.2 266,412 - -0.7 266,429 + -1.4 266,429 + -2.6 266,429 + -1.1 266,429 + -1.8 266,429 + -1.6 266,429 + +0.1 266,429 + -0.7 266,429 + -0.6 266,429 + -3.4 266,429 + -2.4 266,429 + -0.4 266,430 - -2.6 266,430 - -0.6 266,430 - -0.3 266,430 - -2.8 266,430 - -0.8 266,430 - -1.6 266,430 - -2.1 266,430 - -1.6 266,430 - -1.0 266,431 + -1.5 266,431 + -0.7 266,432 - -1.0 266,839 + COO64_RS13570 0.35 -1.2 266,887 + COO64_RS13570 0.39 +0.8 266,887 + COO64_RS13570 0.39 -0.8 266,887 + COO64_RS13570 0.39 -0.7 266,887 + COO64_RS13570 0.39 +0.4 266,888 - COO64_RS13570 0.39 -0.6 267,160 + COO64_RS13570 0.60 -3.0 267,161 - COO64_RS13570 0.60 -0.6 267,223 - COO64_RS13570 0.65 -1.9 267,301 + COO64_RS13570 0.71 -0.5 267,301 + COO64_RS13570 0.71 -1.6 267,369 + COO64_RS13570 0.77 -2.4 267,653 + -0.5 267,750 + -1.9 267,750 + -1.4 267,750 + -2.0 267,750 + -3.7 267,750 + -2.6 267,750 + -1.9 267,751 - -2.0 267,751 - -2.9 267,751 - -1.1 267,751 - -1.6 267,897 + COO64_RS13575 0.23 -0.7 267,897 + COO64_RS13575 0.23 -1.6 268,002 + COO64_RS13575 0.34 -2.7 268,002 + COO64_RS13575 0.34 -0.5 268,002 + COO64_RS13575 0.34 -1.2 268,003 - COO64_RS13575 0.34 -3.5 268,003 - COO64_RS13575 0.34 -2.2 268,003 - COO64_RS13575 0.34 -2.8 268,017 + COO64_RS13575 0.35 -1.9 268,017 + COO64_RS13575 0.35 -0.8 268,018 - COO64_RS13575 0.35 -1.4
Or see this region's nucleotide sequence