Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phaC and COO64_RS13555 are separated by 349 nucleotides COO64_RS13555 and hslU are separated by 92 nucleotides
COO64_RS13550: phaC - class II poly(R)-hydroxyalkanoic acid synthase, at 261,765 to 263,444
phaC
COO64_RS13555: COO64_RS13555 - gamma-butyrobetaine hydroxylase-like domain-containing protein, at 263,794 to 264,171
_RS13555
COO64_RS13560: hslU - ATP-dependent protease ATPase subunit HslU, at 264,264 to 265,601
hslU
Position (kb) 263
264
265 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 263.117 kb on + strand, within phaC at 263.155 kb on + strand, within phaC at 263.155 kb on + strand, within phaC at 263.156 kb on - strand, within phaC at 263.156 kb on - strand, within phaC at 263.156 kb on - strand, within phaC at 263.156 kb on - strand, within phaC at 263.331 kb on + strand at 263.331 kb on + strand at 263.332 kb on - strand at 263.332 kb on - strand at 263.430 kb on - strand at 263.430 kb on - strand at 263.438 kb on + strand at 263.438 kb on + strand at 263.439 kb on - strand at 263.439 kb on - strand at 263.446 kb on + strand at 263.446 kb on + strand at 263.446 kb on + strand at 263.447 kb on - strand at 263.447 kb on - strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.630 kb on + strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.631 kb on - strand at 263.634 kb on + strand at 263.634 kb on + strand at 263.634 kb on + strand at 263.634 kb on + strand at 263.635 kb on - strand at 263.635 kb on - strand at 263.635 kb on - strand at 263.635 kb on - strand at 263.635 kb on - strand at 263.635 kb on - strand at 263.654 kb on - strand at 263.654 kb on - strand at 263.705 kb on - strand at 263.705 kb on - strand at 263.707 kb on - strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.754 kb on + strand at 263.775 kb on - strand at 263.795 kb on + strand at 263.795 kb on + strand at 263.795 kb on + strand at 263.796 kb on - strand at 263.796 kb on - strand at 263.864 kb on + strand, within COO64_RS13555 at 263.910 kb on - strand, within COO64_RS13555 at 263.910 kb on - strand, within COO64_RS13555 at 263.967 kb on - strand, within COO64_RS13555 at 264.092 kb on + strand, within COO64_RS13555 at 264.093 kb on - strand, within COO64_RS13555 at 264.093 kb on - strand, within COO64_RS13555 at 264.191 kb on - strand at 264.191 kb on - strand at 264.191 kb on - strand at 264.191 kb on - strand at 264.204 kb on + strand at 264.204 kb on + strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.205 kb on - strand at 264.266 kb on - strand at 264.274 kb on + strand at 264.274 kb on + strand at 264.275 kb on - strand at 264.275 kb on - strand at 264.325 kb on + strand at 264.326 kb on - strand at 264.326 kb on - strand at 264.326 kb on - strand at 264.326 kb on - strand at 264.326 kb on - strand at 264.545 kb on - strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.704 kb on + strand, within hslU at 264.705 kb on - strand, within hslU at 264.705 kb on - strand, within hslU at 264.705 kb on - strand, within hslU at 264.705 kb on - strand, within hslU at 264.705 kb on - strand, within hslU
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 263,117 + phaC COO64_RS13550 0.80 -1.2 263,155 + phaC COO64_RS13550 0.83 +0.1 263,155 + phaC COO64_RS13550 0.83 -1.0 263,156 - phaC COO64_RS13550 0.83 -0.4 263,156 - phaC COO64_RS13550 0.83 -0.5 263,156 - phaC COO64_RS13550 0.83 -0.4 263,156 - phaC COO64_RS13550 0.83 -2.0 263,331 + -0.5 263,331 + -0.2 263,332 - +0.0 263,332 - +0.7 263,430 - -1.3 263,430 - -0.1 263,438 + +0.3 263,438 + +0.7 263,439 - -0.0 263,439 - -0.3 263,446 + -0.9 263,446 + -0.5 263,446 + -0.8 263,447 - +1.0 263,447 - +0.0 263,630 + +0.1 263,630 + +0.2 263,630 + +0.4 263,630 + -0.7 263,630 + -0.1 263,630 + -0.8 263,630 + -0.8 263,631 - +1.0 263,631 - +0.1 263,631 - -0.5 263,631 - +0.5 263,631 - +0.3 263,631 - -0.5 263,631 - -0.6 263,634 + +0.3 263,634 + -0.1 263,634 + +0.9 263,634 + +0.4 263,635 - -1.0 263,635 - +0.7 263,635 - +0.3 263,635 - -0.4 263,635 - -0.6 263,635 - -0.6 263,654 - +0.2 263,654 - +1.3 263,705 - +0.9 263,705 - +0.2 263,707 - -0.0 263,754 + -1.1 263,754 + -0.0 263,754 + -0.3 263,754 + -0.0 263,775 - -2.8 263,795 + -0.7 263,795 + -0.9 263,795 + -0.8 263,796 - -0.2 263,796 - +1.3 263,864 + COO64_RS13555 0.19 +0.4 263,910 - COO64_RS13555 0.31 -0.0 263,910 - COO64_RS13555 0.31 -0.8 263,967 - COO64_RS13555 0.46 -1.7 264,092 + COO64_RS13555 0.79 -0.6 264,093 - COO64_RS13555 0.79 -0.8 264,093 - COO64_RS13555 0.79 -0.6 264,191 - +0.2 264,191 - +1.0 264,191 - -0.8 264,191 - +0.4 264,204 + -0.4 264,204 + -0.9 264,205 - -0.2 264,205 - -0.0 264,205 - -1.5 264,205 - -0.3 264,205 - -0.2 264,205 - -0.1 264,205 - -0.7 264,266 - +1.1 264,274 + +0.7 264,274 + -0.9 264,275 - +0.0 264,275 - +0.0 264,325 + +1.0 264,326 - +0.9 264,326 - +0.8 264,326 - +0.8 264,326 - +2.5 264,326 - -0.2 264,545 - hslU COO64_RS13560 0.21 +0.6 264,704 + hslU COO64_RS13560 0.33 -0.2 264,704 + hslU COO64_RS13560 0.33 +2.6 264,704 + hslU COO64_RS13560 0.33 +0.0 264,704 + hslU COO64_RS13560 0.33 -0.2 264,704 + hslU COO64_RS13560 0.33 -1.5 264,704 + hslU COO64_RS13560 0.33 +0.4 264,704 + hslU COO64_RS13560 0.33 -0.7 264,705 - hslU COO64_RS13560 0.33 +0.4 264,705 - hslU COO64_RS13560 0.33 +0.4 264,705 - hslU COO64_RS13560 0.33 +1.0 264,705 - hslU COO64_RS13560 0.33 +1.5 264,705 - hslU COO64_RS13560 0.33 +1.7
Or see this region's nucleotide sequence