Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS13030 and rpmE are separated by 171 nucleotides rpmE and COO64_RS13040 are separated by 2 nucleotides COO64_RS13040 and COO64_RS13045 are separated by 179 nucleotides
COO64_RS13030: COO64_RS13030 - primosomal protein N', at 144,955 to 147,174
_RS13030
COO64_RS13035: rpmE - 50S ribosomal protein L31, at 147,346 to 147,561
rpmE
COO64_RS13040: COO64_RS13040 - thermonuclease family protein, at 147,564 to 148,385
_RS13040
COO64_RS13045: COO64_RS13045 - malic enzyme-like NAD(P)-binding protein, at 148,565 to 149,833
_RS13045
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.157 kb on + strand at 147.158 kb on - strand at 147.158 kb on - strand at 147.218 kb on - strand at 147.218 kb on - strand at 147.218 kb on - strand at 147.236 kb on + strand at 147.237 kb on - strand at 147.237 kb on - strand at 147.237 kb on - strand at 147.237 kb on - strand at 147.283 kb on + strand at 147.559 kb on + strand at 147.559 kb on + strand at 147.560 kb on - strand at 147.726 kb on - strand, within COO64_RS13040 at 147.726 kb on - strand, within COO64_RS13040 at 147.767 kb on + strand, within COO64_RS13040 at 147.768 kb on - strand, within COO64_RS13040 at 147.907 kb on - strand, within COO64_RS13040 at 147.907 kb on - strand, within COO64_RS13040 at 147.930 kb on + strand, within COO64_RS13040 at 148.129 kb on + strand, within COO64_RS13040 at 148.129 kb on + strand, within COO64_RS13040 at 148.129 kb on + strand, within COO64_RS13040 at 148.129 kb on + strand, within COO64_RS13040 at 148.130 kb on - strand, within COO64_RS13040 at 148.130 kb on - strand, within COO64_RS13040 at 148.130 kb on - strand, within COO64_RS13040 at 148.130 kb on - strand, within COO64_RS13040 at 148.130 kb on - strand, within COO64_RS13040 at 148.384 kb on - strand at 148.417 kb on - strand at 148.466 kb on + strand at 148.520 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.545 kb on + strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.546 kb on - strand at 148.550 kb on - strand at 148.550 kb on - strand at 148.595 kb on + strand at 148.596 kb on - strand at 148.646 kb on - strand at 148.677 kb on - strand at 148.701 kb on + strand, within COO64_RS13045 at 148.733 kb on + strand, within COO64_RS13045 at 148.733 kb on + strand, within COO64_RS13045 at 148.733 kb on + strand, within COO64_RS13045 at 148.734 kb on - strand, within COO64_RS13045 at 148.739 kb on + strand, within COO64_RS13045 at 148.739 kb on + strand, within COO64_RS13045 at 148.739 kb on + strand, within COO64_RS13045 at 148.739 kb on + strand, within COO64_RS13045 at 148.740 kb on - strand, within COO64_RS13045 at 148.740 kb on - strand, within COO64_RS13045 at 148.740 kb on - strand, within COO64_RS13045 at 148.936 kb on + strand, within COO64_RS13045 at 148.936 kb on + strand, within COO64_RS13045 at 148.936 kb on + strand, within COO64_RS13045 at 148.936 kb on + strand, within COO64_RS13045 at 148.936 kb on + strand, within COO64_RS13045 at 148.937 kb on - strand, within COO64_RS13045 at 148.937 kb on - strand, within COO64_RS13045 at 149.265 kb on - strand, within COO64_RS13045 at 149.265 kb on - strand, within COO64_RS13045
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 147,157 + +1.2 147,157 + -0.7 147,157 + +0.0 147,157 + -0.1 147,157 + -0.5 147,157 + +0.7 147,157 + +0.4 147,157 + -0.1 147,158 - +0.2 147,158 - +0.9 147,218 - +0.6 147,218 - -0.8 147,218 - -0.7 147,236 + +0.5 147,237 - +0.7 147,237 - -1.1 147,237 - -0.5 147,237 - -0.4 147,283 + -3.2 147,559 + -0.9 147,559 + +0.2 147,560 - -1.1 147,726 - COO64_RS13040 0.20 +0.5 147,726 - COO64_RS13040 0.20 -2.5 147,767 + COO64_RS13040 0.25 -1.0 147,768 - COO64_RS13040 0.25 -0.2 147,907 - COO64_RS13040 0.42 -0.7 147,907 - COO64_RS13040 0.42 -1.2 147,930 + COO64_RS13040 0.45 +0.2 148,129 + COO64_RS13040 0.69 +0.2 148,129 + COO64_RS13040 0.69 -1.1 148,129 + COO64_RS13040 0.69 +0.5 148,129 + COO64_RS13040 0.69 -1.0 148,130 - COO64_RS13040 0.69 -0.5 148,130 - COO64_RS13040 0.69 -1.3 148,130 - COO64_RS13040 0.69 +1.2 148,130 - COO64_RS13040 0.69 -0.2 148,130 - COO64_RS13040 0.69 -0.9 148,384 - -1.0 148,417 - -0.3 148,466 + -1.7 148,520 + -0.1 148,545 + +0.0 148,545 + +0.1 148,545 + -0.2 148,545 + -0.8 148,545 + -0.6 148,545 + +0.6 148,545 + +0.9 148,545 + +0.7 148,546 - -1.0 148,546 - -0.5 148,546 - +0.0 148,546 - -1.3 148,546 - -0.5 148,546 - -1.4 148,546 - -1.6 148,546 - -2.0 148,546 - -1.8 148,546 - +0.1 148,546 - -0.7 148,550 - -1.5 148,550 - -1.3 148,595 + -1.3 148,596 - -0.5 148,646 - -2.7 148,677 - -0.8 148,701 + COO64_RS13045 0.11 -2.8 148,733 + COO64_RS13045 0.13 -1.5 148,733 + COO64_RS13045 0.13 -0.3 148,733 + COO64_RS13045 0.13 -2.0 148,734 - COO64_RS13045 0.13 -0.8 148,739 + COO64_RS13045 0.14 -1.2 148,739 + COO64_RS13045 0.14 -1.4 148,739 + COO64_RS13045 0.14 -1.1 148,739 + COO64_RS13045 0.14 -1.3 148,740 - COO64_RS13045 0.14 -1.4 148,740 - COO64_RS13045 0.14 -1.0 148,740 - COO64_RS13045 0.14 +0.8 148,936 + COO64_RS13045 0.29 -1.1 148,936 + COO64_RS13045 0.29 -1.3 148,936 + COO64_RS13045 0.29 -1.3 148,936 + COO64_RS13045 0.29 -0.3 148,936 + COO64_RS13045 0.29 -0.8 148,937 - COO64_RS13045 0.29 -1.0 148,937 - COO64_RS13045 0.29 -0.8 149,265 - COO64_RS13045 0.55 -1.4 149,265 - COO64_RS13045 0.55 -2.2
Or see this region's nucleotide sequence