Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gabD and gabT are separated by 183 nucleotides gabT and COO64_RS12485 are separated by 141 nucleotides
COO64_RS12475: gabD - NADP-dependent succinate-semialdehyde dehydrogenase, at 28,602 to 30,044
gabD
COO64_RS12480: gabT - 4-aminobutyrate--2-oxoglutarate transaminase, at 30,228 to 31,508
gabT
COO64_RS12485: COO64_RS12485 - response regulator, at 31,650 to 32,858
_RS12485
Position (kb) 30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 29.445 kb on + strand, within gabD at 29.445 kb on + strand, within gabD at 29.446 kb on - strand, within gabD at 29.446 kb on - strand, within gabD at 29.483 kb on + strand, within gabD at 29.483 kb on + strand, within gabD at 29.483 kb on + strand, within gabD at 29.483 kb on + strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.484 kb on - strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.487 kb on + strand, within gabD at 29.488 kb on - strand, within gabD at 29.488 kb on - strand, within gabD at 29.488 kb on - strand, within gabD at 29.488 kb on - strand, within gabD at 29.505 kb on + strand, within gabD at 29.505 kb on + strand, within gabD at 29.505 kb on + strand, within gabD at 29.505 kb on + strand, within gabD at 29.506 kb on - strand, within gabD at 29.662 kb on + strand, within gabD at 29.663 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.691 kb on - strand, within gabD at 29.844 kb on + strand, within gabD at 29.844 kb on + strand, within gabD at 29.844 kb on + strand, within gabD at 29.844 kb on + strand, within gabD at 29.844 kb on + strand, within gabD at 29.844 kb on + strand, within gabD at 29.845 kb on - strand, within gabD at 29.845 kb on - strand, within gabD at 29.845 kb on - strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.850 kb on + strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.851 kb on - strand, within gabD at 29.898 kb on + strand, within gabD at 29.898 kb on + strand, within gabD at 29.898 kb on + strand, within gabD at 29.899 kb on - strand, within gabD at 29.899 kb on - strand, within gabD at 29.994 kb on + strand at 29.994 kb on + strand at 29.994 kb on + strand at 29.995 kb on - strand at 29.995 kb on - strand at 29.995 kb on - strand at 30.010 kb on - strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.024 kb on + strand at 30.025 kb on - strand at 30.025 kb on - strand at 30.025 kb on - strand at 30.025 kb on - strand at 30.053 kb on + strand at 30.054 kb on - strand at 30.054 kb on - strand at 30.054 kb on - strand at 30.054 kb on - strand at 30.061 kb on + strand at 30.061 kb on + strand at 30.062 kb on - strand at 30.062 kb on - strand at 30.062 kb on - strand at 30.106 kb on + strand at 30.107 kb on - strand at 30.107 kb on - strand at 30.113 kb on + strand at 30.113 kb on + strand at 30.113 kb on + strand at 30.114 kb on - strand at 30.114 kb on - strand at 30.114 kb on - strand at 30.114 kb on - strand at 30.114 kb on - strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.227 kb on + strand at 30.228 kb on - strand at 30.228 kb on - strand at 30.228 kb on - strand at 30.228 kb on - strand at 30.228 kb on - strand at 30.385 kb on + strand, within gabT at 30.385 kb on + strand, within gabT at 30.385 kb on + strand, within gabT at 30.386 kb on - strand, within gabT at 30.455 kb on + strand, within gabT at 30.455 kb on + strand, within gabT at 30.456 kb on - strand, within gabT at 30.456 kb on - strand, within gabT at 30.477 kb on + strand, within gabT at 30.477 kb on + strand, within gabT at 30.477 kb on + strand, within gabT at 30.478 kb on - strand, within gabT at 30.478 kb on - strand, within gabT at 30.478 kb on - strand, within gabT at 30.486 kb on + strand, within gabT at 30.486 kb on + strand, within gabT at 30.486 kb on + strand, within gabT at 30.486 kb on + strand, within gabT at 30.487 kb on - strand, within gabT at 30.487 kb on - strand, within gabT at 30.487 kb on - strand, within gabT at 30.487 kb on - strand, within gabT at 30.487 kb on - strand, within gabT at 30.520 kb on + strand, within gabT at 30.521 kb on - strand, within gabT at 30.521 kb on - strand, within gabT at 30.521 kb on - strand, within gabT at 30.521 kb on - strand, within gabT at 30.521 kb on - strand, within gabT at 30.643 kb on - strand, within gabT at 30.654 kb on - strand, within gabT at 30.654 kb on - strand, within gabT at 30.680 kb on + strand, within gabT at 30.741 kb on + strand, within gabT at 30.797 kb on + strand, within gabT at 30.797 kb on + strand, within gabT at 30.797 kb on + strand, within gabT at 30.798 kb on - strand, within gabT at 30.798 kb on - strand, within gabT at 30.798 kb on - strand, within gabT at 30.798 kb on - strand, within gabT at 30.798 kb on - strand, within gabT at 30.798 kb on - strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.873 kb on + strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 30.874 kb on - strand, within gabT at 31.083 kb on + strand, within gabT at 31.083 kb on + strand, within gabT at 31.083 kb on + strand, within gabT at 31.084 kb on - strand, within gabT at 31.122 kb on + strand, within gabT at 31.123 kb on - strand, within gabT at 31.123 kb on - strand, within gabT at 31.407 kb on + strand at 31.407 kb on + strand at 31.407 kb on + strand at 31.408 kb on - strand at 31.408 kb on - strand at 31.425 kb on - strand at 31.521 kb on + strand at 31.521 kb on + strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.522 kb on - strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.577 kb on + strand at 31.578 kb on - strand at 31.578 kb on - strand at 31.578 kb on - strand at 31.578 kb on - strand at 31.578 kb on - strand at 31.578 kb on - strand at 31.587 kb on + strand at 31.587 kb on + strand at 31.587 kb on + strand at 31.587 kb on + strand at 31.587 kb on + strand at 31.587 kb on + strand at 31.588 kb on - strand at 31.588 kb on - strand at 31.588 kb on - strand at 31.588 kb on - strand at 31.588 kb on - strand at 31.649 kb on + strand at 31.649 kb on + strand at 31.649 kb on + strand at 31.649 kb on + strand at 31.649 kb on + strand at 31.649 kb on + strand at 31.650 kb on - strand at 31.650 kb on - strand at 31.650 kb on - strand at 31.650 kb on - strand at 31.650 kb on - strand at 31.650 kb on - strand at 31.660 kb on + strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.661 kb on - strand at 31.977 kb on + strand, within COO64_RS12485 at 31.978 kb on - strand, within COO64_RS12485 at 31.978 kb on - strand, within COO64_RS12485 at 32.204 kb on - strand, within COO64_RS12485 at 32.372 kb on + strand, within COO64_RS12485 at 32.372 kb on + strand, within COO64_RS12485 at 32.373 kb on - strand, within COO64_RS12485 at 32.496 kb on + strand, within COO64_RS12485 at 32.497 kb on - strand, within COO64_RS12485 at 32.497 kb on - strand, within COO64_RS12485
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 29,445 + gabD COO64_RS12475 0.58 +0.4 29,445 + gabD COO64_RS12475 0.58 -0.3 29,446 - gabD COO64_RS12475 0.58 -0.3 29,446 - gabD COO64_RS12475 0.58 +0.6 29,483 + gabD COO64_RS12475 0.61 +0.5 29,483 + gabD COO64_RS12475 0.61 -0.1 29,483 + gabD COO64_RS12475 0.61 -0.4 29,483 + gabD COO64_RS12475 0.61 -0.0 29,484 - gabD COO64_RS12475 0.61 +0.6 29,484 - gabD COO64_RS12475 0.61 -1.8 29,484 - gabD COO64_RS12475 0.61 +0.0 29,484 - gabD COO64_RS12475 0.61 -0.4 29,484 - gabD COO64_RS12475 0.61 -1.9 29,484 - gabD COO64_RS12475 0.61 +1.0 29,484 - gabD COO64_RS12475 0.61 -0.4 29,484 - gabD COO64_RS12475 0.61 +0.6 29,484 - gabD COO64_RS12475 0.61 -0.3 29,484 - gabD COO64_RS12475 0.61 -0.6 29,484 - gabD COO64_RS12475 0.61 -0.5 29,484 - gabD COO64_RS12475 0.61 -0.3 29,487 + gabD COO64_RS12475 0.61 +0.5 29,487 + gabD COO64_RS12475 0.61 -1.3 29,487 + gabD COO64_RS12475 0.61 +0.3 29,487 + gabD COO64_RS12475 0.61 +1.3 29,487 + gabD COO64_RS12475 0.61 -0.1 29,487 + gabD COO64_RS12475 0.61 -0.6 29,487 + gabD COO64_RS12475 0.61 +1.2 29,488 - gabD COO64_RS12475 0.61 -0.4 29,488 - gabD COO64_RS12475 0.61 -0.8 29,488 - gabD COO64_RS12475 0.61 -0.6 29,488 - gabD COO64_RS12475 0.61 +0.8 29,505 + gabD COO64_RS12475 0.63 +1.8 29,505 + gabD COO64_RS12475 0.63 +0.8 29,505 + gabD COO64_RS12475 0.63 -2.3 29,505 + gabD COO64_RS12475 0.63 -0.5 29,506 - gabD COO64_RS12475 0.63 -0.5 29,662 + gabD COO64_RS12475 0.73 +0.4 29,663 - gabD COO64_RS12475 0.74 -0.1 29,691 - gabD COO64_RS12475 0.75 -0.3 29,691 - gabD COO64_RS12475 0.75 +0.2 29,691 - gabD COO64_RS12475 0.75 -0.9 29,691 - gabD COO64_RS12475 0.75 -0.3 29,691 - gabD COO64_RS12475 0.75 +0.3 29,691 - gabD COO64_RS12475 0.75 -1.0 29,691 - gabD COO64_RS12475 0.75 -2.4 29,844 + gabD COO64_RS12475 0.86 -1.3 29,844 + gabD COO64_RS12475 0.86 -0.1 29,844 + gabD COO64_RS12475 0.86 +0.3 29,844 + gabD COO64_RS12475 0.86 +0.4 29,844 + gabD COO64_RS12475 0.86 -1.2 29,844 + gabD COO64_RS12475 0.86 -0.5 29,845 - gabD COO64_RS12475 0.86 -0.3 29,845 - gabD COO64_RS12475 0.86 -0.5 29,845 - gabD COO64_RS12475 0.86 -0.3 29,850 + gabD COO64_RS12475 0.86 +0.7 29,850 + gabD COO64_RS12475 0.86 +0.3 29,850 + gabD COO64_RS12475 0.86 +0.3 29,850 + gabD COO64_RS12475 0.86 +0.5 29,850 + gabD COO64_RS12475 0.86 -0.6 29,850 + gabD COO64_RS12475 0.86 -0.3 29,850 + gabD COO64_RS12475 0.86 +0.7 29,850 + gabD COO64_RS12475 0.86 -0.7 29,850 + gabD COO64_RS12475 0.86 -0.1 29,850 + gabD COO64_RS12475 0.86 -0.1 29,851 - gabD COO64_RS12475 0.87 +0.6 29,851 - gabD COO64_RS12475 0.87 +0.5 29,851 - gabD COO64_RS12475 0.87 -1.2 29,851 - gabD COO64_RS12475 0.87 +0.0 29,851 - gabD COO64_RS12475 0.87 -0.0 29,851 - gabD COO64_RS12475 0.87 -1.0 29,851 - gabD COO64_RS12475 0.87 -0.8 29,851 - gabD COO64_RS12475 0.87 -0.9 29,898 + gabD COO64_RS12475 0.90 +1.0 29,898 + gabD COO64_RS12475 0.90 -0.3 29,898 + gabD COO64_RS12475 0.90 +0.6 29,899 - gabD COO64_RS12475 0.90 -0.4 29,899 - gabD COO64_RS12475 0.90 -1.5 29,994 + +0.9 29,994 + -0.7 29,994 + -0.3 29,995 - -1.4 29,995 - +0.1 29,995 - +0.0 30,010 - +0.8 30,024 + -0.6 30,024 + +0.7 30,024 + -2.4 30,024 + +0.0 30,024 + -0.1 30,024 + -0.8 30,024 + +0.6 30,024 + -1.1 30,025 - +0.0 30,025 - +0.3 30,025 - -0.7 30,025 - -0.8 30,053 + +0.5 30,054 - -0.1 30,054 - -0.4 30,054 - +0.7 30,054 - -0.3 30,061 + -0.8 30,061 + +1.4 30,062 - +0.2 30,062 - -1.1 30,062 - -1.3 30,106 + -0.4 30,107 - +0.7 30,107 - -0.2 30,113 + -0.3 30,113 + -0.5 30,113 + -0.8 30,114 - -1.0 30,114 - -0.7 30,114 - +0.2 30,114 - +0.5 30,114 - -1.0 30,227 + -0.7 30,227 + +1.2 30,227 + -0.7 30,227 + -1.1 30,227 + +0.2 30,227 + -0.1 30,227 + +0.0 30,227 + -1.6 30,227 + +0.0 30,227 + -3.1 30,227 + -1.1 30,228 - -0.4 30,228 - -1.0 30,228 - -1.5 30,228 - -0.7 30,228 - -1.0 30,385 + gabT COO64_RS12480 0.12 -0.9 30,385 + gabT COO64_RS12480 0.12 -0.3 30,385 + gabT COO64_RS12480 0.12 -2.2 30,386 - gabT COO64_RS12480 0.12 +0.5 30,455 + gabT COO64_RS12480 0.18 -2.6 30,455 + gabT COO64_RS12480 0.18 -1.2 30,456 - gabT COO64_RS12480 0.18 -2.0 30,456 - gabT COO64_RS12480 0.18 -1.2 30,477 + gabT COO64_RS12480 0.19 +0.5 30,477 + gabT COO64_RS12480 0.19 -0.9 30,477 + gabT COO64_RS12480 0.19 -0.5 30,478 - gabT COO64_RS12480 0.20 +0.3 30,478 - gabT COO64_RS12480 0.20 -0.2 30,478 - gabT COO64_RS12480 0.20 -1.1 30,486 + gabT COO64_RS12480 0.20 -0.5 30,486 + gabT COO64_RS12480 0.20 +0.5 30,486 + gabT COO64_RS12480 0.20 -0.7 30,486 + gabT COO64_RS12480 0.20 +0.2 30,487 - gabT COO64_RS12480 0.20 +1.6 30,487 - gabT COO64_RS12480 0.20 -0.2 30,487 - gabT COO64_RS12480 0.20 -2.4 30,487 - gabT COO64_RS12480 0.20 -0.3 30,487 - gabT COO64_RS12480 0.20 -0.3 30,520 + gabT COO64_RS12480 0.23 +0.2 30,521 - gabT COO64_RS12480 0.23 -0.3 30,521 - gabT COO64_RS12480 0.23 -0.5 30,521 - gabT COO64_RS12480 0.23 +1.1 30,521 - gabT COO64_RS12480 0.23 -0.6 30,521 - gabT COO64_RS12480 0.23 -0.8 30,643 - gabT COO64_RS12480 0.32 -0.3 30,654 - gabT COO64_RS12480 0.33 -0.7 30,654 - gabT COO64_RS12480 0.33 -1.1 30,680 + gabT COO64_RS12480 0.35 -1.0 30,741 + gabT COO64_RS12480 0.40 -0.3 30,797 + gabT COO64_RS12480 0.44 -0.7 30,797 + gabT COO64_RS12480 0.44 -0.4 30,797 + gabT COO64_RS12480 0.44 -0.3 30,798 - gabT COO64_RS12480 0.44 -0.8 30,798 - gabT COO64_RS12480 0.44 +0.2 30,798 - gabT COO64_RS12480 0.44 -0.8 30,798 - gabT COO64_RS12480 0.44 -0.5 30,798 - gabT COO64_RS12480 0.44 +0.2 30,798 - gabT COO64_RS12480 0.44 -1.7 30,873 + gabT COO64_RS12480 0.50 +0.2 30,873 + gabT COO64_RS12480 0.50 +0.9 30,873 + gabT COO64_RS12480 0.50 -0.3 30,873 + gabT COO64_RS12480 0.50 +0.7 30,873 + gabT COO64_RS12480 0.50 +0.8 30,873 + gabT COO64_RS12480 0.50 -1.3 30,873 + gabT COO64_RS12480 0.50 -1.4 30,873 + gabT COO64_RS12480 0.50 -0.5 30,873 + gabT COO64_RS12480 0.50 -0.2 30,873 + gabT COO64_RS12480 0.50 -1.0 30,873 + gabT COO64_RS12480 0.50 -0.9 30,874 - gabT COO64_RS12480 0.50 -0.4 30,874 - gabT COO64_RS12480 0.50 -1.1 30,874 - gabT COO64_RS12480 0.50 +0.2 30,874 - gabT COO64_RS12480 0.50 -0.2 30,874 - gabT COO64_RS12480 0.50 -0.3 30,874 - gabT COO64_RS12480 0.50 -0.9 30,874 - gabT COO64_RS12480 0.50 +0.7 30,874 - gabT COO64_RS12480 0.50 -1.1 30,874 - gabT COO64_RS12480 0.50 +0.6 30,874 - gabT COO64_RS12480 0.50 +1.3 30,874 - gabT COO64_RS12480 0.50 +0.2 30,874 - gabT COO64_RS12480 0.50 +0.0 30,874 - gabT COO64_RS12480 0.50 -1.0 31,083 + gabT COO64_RS12480 0.67 +0.5 31,083 + gabT COO64_RS12480 0.67 -1.1 31,083 + gabT COO64_RS12480 0.67 -0.1 31,084 - gabT COO64_RS12480 0.67 +0.2 31,122 + gabT COO64_RS12480 0.70 -0.1 31,123 - gabT COO64_RS12480 0.70 +1.0 31,123 - gabT COO64_RS12480 0.70 -0.8 31,407 + -1.1 31,407 + +0.6 31,407 + -2.4 31,408 - +0.2 31,408 - -0.8 31,425 - -1.1 31,521 + -0.1 31,521 + -0.9 31,522 - -0.5 31,522 - -0.4 31,522 - -1.5 31,522 - +0.7 31,522 - -1.1 31,522 - -0.0 31,522 - -0.6 31,577 + +0.4 31,577 + +0.1 31,577 + -0.2 31,577 + -0.0 31,577 + -0.3 31,577 + -1.1 31,577 + +0.8 31,577 + +0.1 31,577 + +0.3 31,577 + -0.5 31,577 + +0.8 31,578 - +0.8 31,578 - -0.8 31,578 - -0.3 31,578 - +0.2 31,578 - +0.2 31,578 - -2.3 31,587 + -0.1 31,587 + -0.1 31,587 + -0.4 31,587 + +1.0 31,587 + +0.7 31,587 + +1.0 31,588 - -0.6 31,588 - -0.4 31,588 - +0.2 31,588 - -0.7 31,588 - +1.4 31,649 + +0.7 31,649 + -0.4 31,649 + +1.8 31,649 + +0.1 31,649 + +0.8 31,649 + -1.8 31,650 - +0.5 31,650 - -0.2 31,650 - +0.6 31,650 - -0.8 31,650 - -0.6 31,650 - +0.6 31,660 + -1.3 31,661 - +0.5 31,661 - +0.3 31,661 - -1.7 31,661 - +0.9 31,977 + COO64_RS12485 0.27 +0.5 31,978 - COO64_RS12485 0.27 +0.2 31,978 - COO64_RS12485 0.27 +0.6 32,204 - COO64_RS12485 0.46 +1.0 32,372 + COO64_RS12485 0.60 -0.5 32,372 + COO64_RS12485 0.60 -0.3 32,373 - COO64_RS12485 0.60 +1.0 32,496 + COO64_RS12485 0.70 +1.0 32,497 - COO64_RS12485 0.70 +0.5 32,497 - COO64_RS12485 0.70 -0.3
Or see this region's nucleotide sequence