Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS12435 and COO64_RS12440 are separated by 71 nucleotides COO64_RS12440 and COO64_RS12445 are separated by 12 nucleotides COO64_RS12445 and COO64_RS12450 are separated by 174 nucleotides COO64_RS12450 and COO64_RS12455 are separated by 15 nucleotides
COO64_RS12435: COO64_RS12435 - DUF2789 domain-containing protein, at 23,206 to 23,448
_RS12435
COO64_RS12440: COO64_RS12440 - hypothetical protein, at 23,520 to 24,155
_RS12440
COO64_RS12445: COO64_RS12445 - CBS domain-containing protein, at 24,168 to 24,608
_RS12445
COO64_RS12450: COO64_RS12450 - DUF805 domain-containing protein, at 24,783 to 25,232
_RS12450
COO64_RS12455: COO64_RS12455 - hypothetical protein, at 25,248 to 25,601
_RS12455
Position (kb)
24
25 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 23.217 kb on + strand at 23.217 kb on + strand at 23.217 kb on + strand at 23.218 kb on - strand at 23.218 kb on - strand at 23.218 kb on - strand at 23.218 kb on - strand at 23.218 kb on - strand at 23.307 kb on + strand, within COO64_RS12435 at 23.307 kb on + strand, within COO64_RS12435 at 23.318 kb on + strand, within COO64_RS12435 at 23.318 kb on + strand, within COO64_RS12435 at 23.318 kb on + strand, within COO64_RS12435 at 23.319 kb on - strand, within COO64_RS12435 at 23.319 kb on - strand, within COO64_RS12435 at 23.319 kb on - strand, within COO64_RS12435 at 23.376 kb on + strand, within COO64_RS12435 at 23.376 kb on + strand, within COO64_RS12435 at 23.377 kb on - strand, within COO64_RS12435 at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.485 kb on + strand at 23.486 kb on - strand at 23.486 kb on - strand at 23.486 kb on - strand at 23.486 kb on - strand at 23.486 kb on - strand at 23.533 kb on - strand at 23.586 kb on + strand, within COO64_RS12440 at 23.586 kb on + strand, within COO64_RS12440 at 23.586 kb on + strand, within COO64_RS12440 at 23.587 kb on - strand, within COO64_RS12440 at 23.640 kb on + strand, within COO64_RS12440 at 23.641 kb on - strand, within COO64_RS12440 at 23.641 kb on - strand, within COO64_RS12440 at 23.805 kb on + strand, within COO64_RS12440 at 23.820 kb on + strand, within COO64_RS12440 at 23.820 kb on + strand, within COO64_RS12440 at 23.820 kb on + strand, within COO64_RS12440 at 23.821 kb on - strand, within COO64_RS12440 at 24.085 kb on + strand, within COO64_RS12440 at 24.085 kb on + strand, within COO64_RS12440 at 24.086 kb on - strand, within COO64_RS12440 at 24.086 kb on - strand, within COO64_RS12440 at 24.086 kb on - strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.087 kb on + strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.088 kb on - strand, within COO64_RS12440 at 24.184 kb on + strand at 24.184 kb on + strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.185 kb on - strand at 24.328 kb on + strand, within COO64_RS12445 at 24.328 kb on + strand, within COO64_RS12445 at 24.329 kb on - strand, within COO64_RS12445 at 24.459 kb on + strand, within COO64_RS12445 at 24.460 kb on - strand, within COO64_RS12445 at 24.585 kb on + strand at 24.586 kb on - strand at 24.608 kb on + strand at 24.609 kb on - strand at 24.609 kb on - strand at 24.646 kb on + strand at 24.646 kb on + strand at 24.647 kb on - strand at 24.647 kb on - strand at 24.647 kb on - strand at 24.647 kb on - strand at 24.651 kb on - strand at 24.651 kb on - strand at 24.651 kb on - strand at 24.651 kb on - strand at 24.662 kb on + strand at 24.724 kb on + strand at 24.792 kb on - strand at 24.919 kb on - strand, within COO64_RS12450 at 24.950 kb on + strand, within COO64_RS12450 at 24.950 kb on + strand, within COO64_RS12450 at 24.950 kb on + strand, within COO64_RS12450 at 24.951 kb on - strand, within COO64_RS12450 at 24.952 kb on + strand, within COO64_RS12450 at 24.952 kb on + strand, within COO64_RS12450 at 24.952 kb on + strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 24.953 kb on - strand, within COO64_RS12450 at 25.045 kb on + strand, within COO64_RS12450 at 25.046 kb on - strand, within COO64_RS12450 at 25.081 kb on + strand, within COO64_RS12450 at 25.082 kb on - strand, within COO64_RS12450 at 25.082 kb on - strand, within COO64_RS12450 at 25.092 kb on + strand, within COO64_RS12450 at 25.092 kb on + strand, within COO64_RS12450 at 25.092 kb on + strand, within COO64_RS12450 at 25.092 kb on + strand, within COO64_RS12450 at 25.093 kb on - strand, within COO64_RS12450 at 25.093 kb on - strand, within COO64_RS12450 at 25.102 kb on + strand, within COO64_RS12450 at 25.103 kb on - strand, within COO64_RS12450 at 25.103 kb on - strand, within COO64_RS12450 at 25.227 kb on - strand at 25.244 kb on + strand at 25.245 kb on - strand at 25.245 kb on - strand at 25.245 kb on - strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.249 kb on + strand at 25.250 kb on - strand at 25.250 kb on - strand at 25.253 kb on + strand at 25.253 kb on + strand at 25.253 kb on + strand at 25.254 kb on - strand at 25.260 kb on + strand at 25.269 kb on - strand at 25.270 kb on + strand at 25.271 kb on - strand at 25.271 kb on - strand at 25.271 kb on - strand at 25.271 kb on - strand at 25.287 kb on - strand, within COO64_RS12455 at 25.287 kb on - strand, within COO64_RS12455 at 25.386 kb on + strand, within COO64_RS12455 at 25.386 kb on + strand, within COO64_RS12455 at 25.404 kb on - strand, within COO64_RS12455 at 25.404 kb on - strand, within COO64_RS12455 at 25.520 kb on - strand, within COO64_RS12455 at 25.520 kb on - strand, within COO64_RS12455 at 25.520 kb on - strand, within COO64_RS12455
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 23,217 + -2.0 23,217 + -0.1 23,217 + +0.5 23,218 - +0.3 23,218 - -0.3 23,218 - +0.3 23,218 - -0.6 23,218 - +0.3 23,307 + COO64_RS12435 0.42 -0.3 23,307 + COO64_RS12435 0.42 +0.1 23,318 + COO64_RS12435 0.46 +0.7 23,318 + COO64_RS12435 0.46 -0.5 23,318 + COO64_RS12435 0.46 -0.5 23,319 - COO64_RS12435 0.47 -1.2 23,319 - COO64_RS12435 0.47 +1.8 23,319 - COO64_RS12435 0.47 +0.4 23,376 + COO64_RS12435 0.70 -1.3 23,376 + COO64_RS12435 0.70 +0.5 23,377 - COO64_RS12435 0.70 +0.0 23,485 + -0.4 23,485 + -0.5 23,485 + +0.7 23,485 + -0.4 23,485 + -2.0 23,485 + -1.9 23,485 + +0.2 23,485 + -2.0 23,485 + -1.5 23,485 + -0.5 23,485 + +0.2 23,485 + -0.9 23,486 - +0.6 23,486 - -0.2 23,486 - -1.3 23,486 - +1.1 23,486 - +0.1 23,533 - -0.8 23,586 + COO64_RS12440 0.10 -0.8 23,586 + COO64_RS12440 0.10 +0.5 23,586 + COO64_RS12440 0.10 -0.4 23,587 - COO64_RS12440 0.11 +0.0 23,640 + COO64_RS12440 0.19 -1.7 23,641 - COO64_RS12440 0.19 -0.2 23,641 - COO64_RS12440 0.19 +0.8 23,805 + COO64_RS12440 0.45 -0.4 23,820 + COO64_RS12440 0.47 +0.7 23,820 + COO64_RS12440 0.47 -0.8 23,820 + COO64_RS12440 0.47 -0.5 23,821 - COO64_RS12440 0.47 -0.4 24,085 + COO64_RS12440 0.89 +0.5 24,085 + COO64_RS12440 0.89 -0.1 24,086 - COO64_RS12440 0.89 -0.7 24,086 - COO64_RS12440 0.89 +0.2 24,086 - COO64_RS12440 0.89 +0.5 24,087 + COO64_RS12440 0.89 -1.0 24,087 + COO64_RS12440 0.89 -0.5 24,087 + COO64_RS12440 0.89 +0.3 24,087 + COO64_RS12440 0.89 -0.9 24,087 + COO64_RS12440 0.89 -0.5 24,087 + COO64_RS12440 0.89 +0.5 24,088 - COO64_RS12440 0.89 -1.3 24,088 - COO64_RS12440 0.89 -0.4 24,088 - COO64_RS12440 0.89 +0.4 24,088 - COO64_RS12440 0.89 -0.6 24,088 - COO64_RS12440 0.89 -0.9 24,088 - COO64_RS12440 0.89 -0.1 24,088 - COO64_RS12440 0.89 +0.0 24,088 - COO64_RS12440 0.89 +0.2 24,088 - COO64_RS12440 0.89 +0.8 24,088 - COO64_RS12440 0.89 +0.3 24,088 - COO64_RS12440 0.89 -1.0 24,088 - COO64_RS12440 0.89 +0.2 24,088 - COO64_RS12440 0.89 -0.3 24,184 + -1.0 24,184 + -0.9 24,185 - -0.9 24,185 - -0.2 24,185 - +0.5 24,185 - -0.2 24,185 - +0.8 24,185 - +0.7 24,328 + COO64_RS12445 0.36 -0.8 24,328 + COO64_RS12445 0.36 -1.0 24,329 - COO64_RS12445 0.37 -0.1 24,459 + COO64_RS12445 0.66 -0.8 24,460 - COO64_RS12445 0.66 -1.0 24,585 + +0.6 24,586 - +0.8 24,608 + -1.4 24,609 - +0.7 24,609 - +0.2 24,646 + +0.6 24,646 + -0.8 24,647 - +0.5 24,647 - +0.5 24,647 - -0.2 24,647 - -0.6 24,651 - +1.0 24,651 - -0.4 24,651 - -0.0 24,651 - +0.2 24,662 + +0.2 24,724 + -0.2 24,792 - +0.7 24,919 - COO64_RS12450 0.30 +0.5 24,950 + COO64_RS12450 0.37 -1.0 24,950 + COO64_RS12450 0.37 -0.4 24,950 + COO64_RS12450 0.37 +0.3 24,951 - COO64_RS12450 0.37 -1.1 24,952 + COO64_RS12450 0.38 -0.2 24,952 + COO64_RS12450 0.38 -0.5 24,952 + COO64_RS12450 0.38 +0.3 24,953 - COO64_RS12450 0.38 -0.5 24,953 - COO64_RS12450 0.38 -0.5 24,953 - COO64_RS12450 0.38 +0.2 24,953 - COO64_RS12450 0.38 +0.4 24,953 - COO64_RS12450 0.38 -0.3 24,953 - COO64_RS12450 0.38 -1.7 24,953 - COO64_RS12450 0.38 -0.3 24,953 - COO64_RS12450 0.38 +1.0 24,953 - COO64_RS12450 0.38 +1.0 25,045 + COO64_RS12450 0.58 +0.0 25,046 - COO64_RS12450 0.58 -0.3 25,081 + COO64_RS12450 0.66 +0.1 25,082 - COO64_RS12450 0.66 -0.3 25,082 - COO64_RS12450 0.66 -0.1 25,092 + COO64_RS12450 0.69 -0.1 25,092 + COO64_RS12450 0.69 +0.9 25,092 + COO64_RS12450 0.69 -0.3 25,092 + COO64_RS12450 0.69 +0.1 25,093 - COO64_RS12450 0.69 +1.0 25,093 - COO64_RS12450 0.69 +0.2 25,102 + COO64_RS12450 0.71 +0.2 25,103 - COO64_RS12450 0.71 +1.1 25,103 - COO64_RS12450 0.71 -0.5 25,227 - +0.8 25,244 + +0.3 25,245 - +1.7 25,245 - +0.0 25,245 - +0.7 25,249 + -0.3 25,249 + -0.5 25,249 + -0.7 25,249 + -0.4 25,249 + +0.8 25,249 + -0.6 25,249 + +0.9 25,250 - -0.3 25,250 - +0.9 25,253 + -1.0 25,253 + -0.8 25,253 + +0.5 25,254 - -1.4 25,260 + +0.7 25,269 - -0.4 25,270 + -0.1 25,271 - -0.7 25,271 - +0.2 25,271 - -0.2 25,271 - -0.8 25,287 - COO64_RS12455 0.11 +0.0 25,287 - COO64_RS12455 0.11 -0.6 25,386 + COO64_RS12455 0.39 +0.2 25,386 + COO64_RS12455 0.39 +1.9 25,404 - COO64_RS12455 0.44 +0.0 25,404 - COO64_RS12455 0.44 +0.1 25,520 - COO64_RS12455 0.77 -0.4 25,520 - COO64_RS12455 0.77 +0.6 25,520 - COO64_RS12455 0.77 +0.2
Or see this region's nucleotide sequence