Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS12285 and pcaD are separated by 11 nucleotides pcaD and pcaC are separated by 2 nucleotides pcaC and COO64_RS12300 are separated by 80 nucleotides COO64_RS12300 and COO64_RS12305 are separated by 88 nucleotides
COO64_RS12285: COO64_RS12285 - 3-carboxy-cis,cis-muconate cycloisomerase, at 277,249 to 278,628
_RS12285
COO64_RS12290: pcaD - 3-oxoadipate enol-lactonase, at 278,640 to 279,437
pcaD
COO64_RS12295: pcaC - 4-carboxymuconolactone decarboxylase, at 279,440 to 279,832
pcaC
COO64_RS12300: COO64_RS12300 - DUF962 domain-containing protein, at 279,913 to 280,437
_RS12300
COO64_RS12305: COO64_RS12305 - Crp/Fnr family transcriptional regulator, at 280,526 to 281,215
_RS12305
Position (kb) 279
280 Strain fitness (log2 ratio) -2
-1
0
1
2 at 278.563 kb on + strand at 278.563 kb on + strand at 278.564 kb on - strand at 278.564 kb on - strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.650 kb on + strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.651 kb on - strand at 278.668 kb on + strand at 278.669 kb on - strand at 278.669 kb on - strand at 278.669 kb on - strand at 278.676 kb on + strand at 278.676 kb on + strand at 278.676 kb on + strand at 278.677 kb on - strand at 278.677 kb on - strand at 278.677 kb on - strand at 278.841 kb on + strand, within pcaD at 278.841 kb on + strand, within pcaD at 278.842 kb on - strand, within pcaD at 279.121 kb on - strand, within pcaD at 279.121 kb on - strand, within pcaD at 279.162 kb on - strand, within pcaD at 279.198 kb on - strand, within pcaD at 279.458 kb on + strand at 279.458 kb on + strand at 279.459 kb on - strand at 279.459 kb on - strand at 279.583 kb on + strand, within pcaC at 279.584 kb on - strand, within pcaC at 279.749 kb on + strand, within pcaC at 279.749 kb on + strand, within pcaC at 279.750 kb on - strand, within pcaC at 279.750 kb on - strand, within pcaC at 279.750 kb on - strand, within pcaC at 279.866 kb on - strand at 279.866 kb on - strand at 279.866 kb on - strand at 279.866 kb on - strand at 279.866 kb on - strand at 280.082 kb on + strand, within COO64_RS12300 at 280.082 kb on + strand, within COO64_RS12300 at 280.082 kb on + strand, within COO64_RS12300 at 280.083 kb on - strand, within COO64_RS12300 at 280.083 kb on - strand, within COO64_RS12300 at 280.083 kb on - strand, within COO64_RS12300 at 280.204 kb on + strand, within COO64_RS12300 at 280.205 kb on - strand, within COO64_RS12300 at 280.244 kb on + strand, within COO64_RS12300 at 280.244 kb on + strand, within COO64_RS12300 at 280.244 kb on + strand, within COO64_RS12300 at 280.244 kb on + strand, within COO64_RS12300 at 280.245 kb on - strand, within COO64_RS12300 at 280.245 kb on - strand, within COO64_RS12300 at 280.357 kb on - strand, within COO64_RS12300 at 280.397 kb on + strand at 280.397 kb on + strand at 280.397 kb on + strand at 280.398 kb on - strand at 280.406 kb on + strand at 280.407 kb on - strand at 280.452 kb on + strand at 280.452 kb on + strand at 280.453 kb on - strand at 280.473 kb on - strand at 280.525 kb on + strand at 280.585 kb on + strand at 280.631 kb on + strand, within COO64_RS12305 at 280.631 kb on + strand, within COO64_RS12305 at 280.632 kb on - strand, within COO64_RS12305 at 280.667 kb on + strand, within COO64_RS12305 at 280.668 kb on - strand, within COO64_RS12305
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 278,563 + -0.4 278,563 + -2.2 278,564 - -0.9 278,564 - -0.2 278,650 + +0.6 278,650 + -0.3 278,650 + +0.5 278,650 + -0.4 278,650 + +0.5 278,650 + -1.6 278,650 + +1.0 278,651 - +0.5 278,651 - +0.1 278,651 - -0.1 278,651 - -0.1 278,651 - -0.4 278,651 - +0.0 278,651 - -0.8 278,651 - +0.4 278,651 - -0.0 278,651 - +0.6 278,668 + -0.5 278,669 - +0.1 278,669 - -0.5 278,669 - -0.2 278,676 + +0.9 278,676 + +1.6 278,676 + +0.0 278,677 - -0.4 278,677 - +1.5 278,677 - +0.9 278,841 + pcaD COO64_RS12290 0.25 -0.5 278,841 + pcaD COO64_RS12290 0.25 -1.1 278,842 - pcaD COO64_RS12290 0.25 -0.2 279,121 - pcaD COO64_RS12290 0.60 +0.0 279,121 - pcaD COO64_RS12290 0.60 +0.6 279,162 - pcaD COO64_RS12290 0.65 +0.9 279,198 - pcaD COO64_RS12290 0.70 -0.2 279,458 + -0.9 279,458 + -0.1 279,459 - +0.0 279,459 - -1.7 279,583 + pcaC COO64_RS12295 0.36 -0.3 279,584 - pcaC COO64_RS12295 0.37 -0.5 279,749 + pcaC COO64_RS12295 0.79 -0.5 279,749 + pcaC COO64_RS12295 0.79 -0.2 279,750 - pcaC COO64_RS12295 0.79 -1.7 279,750 - pcaC COO64_RS12295 0.79 +1.0 279,750 - pcaC COO64_RS12295 0.79 -0.4 279,866 - +0.8 279,866 - +2.0 279,866 - -0.1 279,866 - -0.4 279,866 - +1.0 280,082 + COO64_RS12300 0.32 +0.3 280,082 + COO64_RS12300 0.32 +0.9 280,082 + COO64_RS12300 0.32 +1.2 280,083 - COO64_RS12300 0.32 -0.1 280,083 - COO64_RS12300 0.32 +0.2 280,083 - COO64_RS12300 0.32 -1.0 280,204 + COO64_RS12300 0.55 +1.4 280,205 - COO64_RS12300 0.56 +0.7 280,244 + COO64_RS12300 0.63 -0.0 280,244 + COO64_RS12300 0.63 -0.6 280,244 + COO64_RS12300 0.63 +0.8 280,244 + COO64_RS12300 0.63 +0.0 280,245 - COO64_RS12300 0.63 -0.3 280,245 - COO64_RS12300 0.63 -0.3 280,357 - COO64_RS12300 0.85 -0.3 280,397 + +0.3 280,397 + +0.8 280,397 + -0.0 280,398 - -0.2 280,406 + +0.2 280,407 - +0.0 280,452 + +0.3 280,452 + -1.0 280,453 - +0.1 280,473 - +1.0 280,525 + -0.2 280,585 + +0.0 280,631 + COO64_RS12305 0.15 +1.0 280,631 + COO64_RS12305 0.15 -1.3 280,632 - COO64_RS12305 0.15 +0.1 280,667 + COO64_RS12305 0.20 +1.1 280,668 - COO64_RS12305 0.21 +0.1
Or see this region's nucleotide sequence