Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS11965 and COO64_RS11970 are separated by 51 nucleotides COO64_RS11970 and COO64_RS11975 are separated by 113 nucleotides COO64_RS11975 and COO64_RS11980 are separated by 116 nucleotides
COO64_RS11965: COO64_RS11965 - ATP-binding protein, at 208,564 to 210,576
_RS11965
COO64_RS11970: COO64_RS11970 - putative 2-dehydropantoate 2-reductase, at 210,628 to 211,545
_RS11970
COO64_RS11975: COO64_RS11975 - YajQ family cyclic di-GMP-binding protein, at 211,659 to 212,144
_RS11975
COO64_RS11980: COO64_RS11980 - mechanosensitive ion channel family protein, at 212,261 to 213,103
_RS11980
Position (kb) 210
211
212 Strain fitness (log2 ratio) -2
-1
0
1
2 at 209.654 kb on + strand, within COO64_RS11965 at 209.654 kb on + strand, within COO64_RS11965 at 209.654 kb on + strand, within COO64_RS11965 at 209.655 kb on - strand, within COO64_RS11965 at 209.655 kb on - strand, within COO64_RS11965 at 209.655 kb on - strand, within COO64_RS11965 at 209.655 kb on - strand, within COO64_RS11965 at 209.655 kb on - strand, within COO64_RS11965 at 209.715 kb on - strand, within COO64_RS11965 at 209.715 kb on - strand, within COO64_RS11965 at 209.715 kb on - strand, within COO64_RS11965 at 209.755 kb on + strand, within COO64_RS11965 at 209.756 kb on - strand, within COO64_RS11965 at 209.825 kb on + strand, within COO64_RS11965 at 209.826 kb on - strand, within COO64_RS11965 at 209.826 kb on - strand, within COO64_RS11965 at 209.880 kb on + strand, within COO64_RS11965 at 209.957 kb on + strand, within COO64_RS11965 at 209.957 kb on + strand, within COO64_RS11965 at 209.958 kb on - strand, within COO64_RS11965 at 210.002 kb on + strand, within COO64_RS11965 at 210.002 kb on + strand, within COO64_RS11965 at 210.003 kb on - strand, within COO64_RS11965 at 210.060 kb on + strand, within COO64_RS11965 at 210.104 kb on + strand, within COO64_RS11965 at 210.104 kb on + strand, within COO64_RS11965 at 210.104 kb on + strand, within COO64_RS11965 at 210.105 kb on - strand, within COO64_RS11965 at 210.105 kb on - strand, within COO64_RS11965 at 210.296 kb on + strand, within COO64_RS11965 at 210.323 kb on + strand, within COO64_RS11965 at 210.323 kb on + strand, within COO64_RS11965 at 210.323 kb on + strand, within COO64_RS11965 at 210.323 kb on + strand, within COO64_RS11965 at 210.323 kb on + strand, within COO64_RS11965 at 210.324 kb on - strand, within COO64_RS11965 at 210.324 kb on - strand, within COO64_RS11965 at 210.324 kb on - strand, within COO64_RS11965 at 210.324 kb on - strand, within COO64_RS11965 at 210.324 kb on - strand, within COO64_RS11965 at 210.472 kb on - strand at 210.472 kb on - strand at 210.588 kb on - strand at 210.600 kb on + strand at 210.600 kb on + strand at 210.601 kb on - strand at 210.601 kb on - strand at 210.601 kb on - strand at 210.601 kb on - strand at 210.746 kb on + strand, within COO64_RS11970 at 210.747 kb on - strand, within COO64_RS11970 at 210.747 kb on - strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.785 kb on + strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.786 kb on - strand, within COO64_RS11970 at 210.797 kb on + strand, within COO64_RS11970 at 210.797 kb on + strand, within COO64_RS11970 at 210.797 kb on + strand, within COO64_RS11970 at 210.797 kb on + strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.798 kb on - strand, within COO64_RS11970 at 210.873 kb on - strand, within COO64_RS11970 at 210.958 kb on + strand, within COO64_RS11970 at 210.958 kb on + strand, within COO64_RS11970 at 211.177 kb on + strand, within COO64_RS11970 at 211.178 kb on - strand, within COO64_RS11970 at 211.178 kb on - strand, within COO64_RS11970 at 211.304 kb on + strand, within COO64_RS11970 at 211.305 kb on - strand, within COO64_RS11970 at 211.424 kb on + strand, within COO64_RS11970 at 211.424 kb on + strand, within COO64_RS11970 at 211.424 kb on + strand, within COO64_RS11970 at 211.424 kb on + strand, within COO64_RS11970 at 211.425 kb on - strand, within COO64_RS11970 at 211.563 kb on + strand at 211.563 kb on + strand at 211.564 kb on - strand at 211.564 kb on - strand at 211.564 kb on - strand at 211.564 kb on - strand at 211.564 kb on - strand at 211.564 kb on - strand at 211.565 kb on + strand at 211.565 kb on + strand at 211.565 kb on + strand at 211.565 kb on + strand at 211.566 kb on - strand at 211.566 kb on - strand at 211.566 kb on - strand at 211.566 kb on - strand at 211.566 kb on - strand at 211.566 kb on - strand at 211.582 kb on - strand at 211.658 kb on + strand at 211.658 kb on + strand at 211.659 kb on - strand at 211.678 kb on + strand at 211.747 kb on - strand, within COO64_RS11975 at 211.962 kb on - strand, within COO64_RS11975 at 212.041 kb on + strand, within COO64_RS11975 at 212.042 kb on - strand, within COO64_RS11975 at 212.255 kb on + strand at 212.255 kb on + strand at 212.255 kb on + strand at 212.255 kb on + strand at 212.256 kb on - strand at 212.256 kb on - strand at 212.268 kb on + strand at 212.331 kb on - strand at 212.331 kb on - strand at 212.399 kb on + strand, within COO64_RS11980 at 212.455 kb on + strand, within COO64_RS11980 at 212.455 kb on + strand, within COO64_RS11980 at 212.456 kb on - strand, within COO64_RS11980 at 212.456 kb on - strand, within COO64_RS11980
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 209,654 + COO64_RS11965 0.54 -1.4 209,654 + COO64_RS11965 0.54 -1.0 209,654 + COO64_RS11965 0.54 -0.8 209,655 - COO64_RS11965 0.54 -0.4 209,655 - COO64_RS11965 0.54 +0.7 209,655 - COO64_RS11965 0.54 -0.1 209,655 - COO64_RS11965 0.54 -0.5 209,655 - COO64_RS11965 0.54 -1.1 209,715 - COO64_RS11965 0.57 +0.5 209,715 - COO64_RS11965 0.57 +0.2 209,715 - COO64_RS11965 0.57 +0.2 209,755 + COO64_RS11965 0.59 -1.3 209,756 - COO64_RS11965 0.59 +0.8 209,825 + COO64_RS11965 0.63 -0.8 209,826 - COO64_RS11965 0.63 -1.0 209,826 - COO64_RS11965 0.63 -0.2 209,880 + COO64_RS11965 0.65 -0.7 209,957 + COO64_RS11965 0.69 -0.1 209,957 + COO64_RS11965 0.69 +0.7 209,958 - COO64_RS11965 0.69 -2.0 210,002 + COO64_RS11965 0.71 -0.8 210,002 + COO64_RS11965 0.71 -0.3 210,003 - COO64_RS11965 0.71 -0.8 210,060 + COO64_RS11965 0.74 -0.2 210,104 + COO64_RS11965 0.77 +0.0 210,104 + COO64_RS11965 0.77 +1.0 210,104 + COO64_RS11965 0.77 +0.5 210,105 - COO64_RS11965 0.77 -0.8 210,105 - COO64_RS11965 0.77 -0.3 210,296 + COO64_RS11965 0.86 -1.5 210,323 + COO64_RS11965 0.87 +0.6 210,323 + COO64_RS11965 0.87 -0.1 210,323 + COO64_RS11965 0.87 +0.2 210,323 + COO64_RS11965 0.87 -1.1 210,323 + COO64_RS11965 0.87 -2.0 210,324 - COO64_RS11965 0.87 +0.8 210,324 - COO64_RS11965 0.87 +0.5 210,324 - COO64_RS11965 0.87 -0.4 210,324 - COO64_RS11965 0.87 -0.1 210,324 - COO64_RS11965 0.87 -0.3 210,472 - +1.5 210,472 - +0.3 210,588 - +0.2 210,600 + -0.6 210,600 + -0.1 210,601 - +0.9 210,601 - +0.7 210,601 - +0.3 210,601 - +0.7 210,746 + COO64_RS11970 0.13 -0.4 210,747 - COO64_RS11970 0.13 -0.3 210,747 - COO64_RS11970 0.13 -1.1 210,785 + COO64_RS11970 0.17 -0.5 210,785 + COO64_RS11970 0.17 -1.9 210,785 + COO64_RS11970 0.17 -1.1 210,785 + COO64_RS11970 0.17 -1.3 210,785 + COO64_RS11970 0.17 +0.3 210,785 + COO64_RS11970 0.17 +1.2 210,786 - COO64_RS11970 0.17 -0.7 210,786 - COO64_RS11970 0.17 -1.1 210,786 - COO64_RS11970 0.17 -0.2 210,786 - COO64_RS11970 0.17 -0.1 210,786 - COO64_RS11970 0.17 +0.2 210,786 - COO64_RS11970 0.17 -0.2 210,797 + COO64_RS11970 0.18 -1.0 210,797 + COO64_RS11970 0.18 -1.0 210,797 + COO64_RS11970 0.18 -1.0 210,797 + COO64_RS11970 0.18 -0.8 210,798 - COO64_RS11970 0.19 -0.4 210,798 - COO64_RS11970 0.19 -0.1 210,798 - COO64_RS11970 0.19 -1.1 210,798 - COO64_RS11970 0.19 -2.5 210,798 - COO64_RS11970 0.19 -1.3 210,798 - COO64_RS11970 0.19 -0.6 210,873 - COO64_RS11970 0.27 +0.0 210,958 + COO64_RS11970 0.36 +1.5 210,958 + COO64_RS11970 0.36 -2.0 211,177 + COO64_RS11970 0.60 +0.0 211,178 - COO64_RS11970 0.60 -0.2 211,178 - COO64_RS11970 0.60 -0.5 211,304 + COO64_RS11970 0.74 -1.3 211,305 - COO64_RS11970 0.74 +0.5 211,424 + COO64_RS11970 0.87 -0.8 211,424 + COO64_RS11970 0.87 -1.3 211,424 + COO64_RS11970 0.87 +0.0 211,424 + COO64_RS11970 0.87 -0.3 211,425 - COO64_RS11970 0.87 -1.0 211,563 + -0.7 211,563 + -1.2 211,564 - +0.8 211,564 - +0.4 211,564 - +0.3 211,564 - -0.6 211,564 - +0.1 211,564 - -1.3 211,565 + -1.4 211,565 + -1.5 211,565 + -2.2 211,565 + +0.2 211,566 - +0.6 211,566 - +0.0 211,566 - -0.4 211,566 - -0.2 211,566 - -0.5 211,566 - +0.2 211,582 - -2.4 211,658 + +0.2 211,658 + -0.3 211,659 - -0.1 211,678 + +0.7 211,747 - COO64_RS11975 0.18 -0.8 211,962 - COO64_RS11975 0.62 -0.8 212,041 + COO64_RS11975 0.79 -0.0 212,042 - COO64_RS11975 0.79 +0.6 212,255 + -0.7 212,255 + +0.5 212,255 + +0.1 212,255 + +1.0 212,256 - -0.5 212,256 - +0.0 212,268 + +0.0 212,331 - -1.5 212,331 - -0.3 212,399 + COO64_RS11980 0.16 +0.5 212,455 + COO64_RS11980 0.23 -1.0 212,455 + COO64_RS11980 0.23 -0.3 212,456 - COO64_RS11980 0.23 +2.0 212,456 - COO64_RS11980 0.23 +0.6
Or see this region's nucleotide sequence