Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdeB and COO64_RS11755 are separated by 154 nucleotides COO64_RS11755 and COO64_RS11760 are separated by 138 nucleotides COO64_RS11760 and COO64_RS11765 are separated by 24 nucleotides
COO64_RS11750: mdeB - alpha-ketoglutarate dehydrogenase, at 163,839 to 166,535
mdeB
COO64_RS11755: COO64_RS11755 - YhcB family protein, at 166,690 to 167,124
_RS11755
COO64_RS11760: COO64_RS11760 - alpha/beta hydrolase, at 167,263 to 167,895
_RS11760
COO64_RS11765: COO64_RS11765 - tryptophan--tRNA ligase, at 167,920 to 169,269
_RS11765
Position (kb) 167
168 Strain fitness (log2 ratio) -2
-1
0
1
2 at 166.287 kb on + strand at 166.287 kb on + strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.288 kb on - strand at 166.320 kb on + strand at 166.320 kb on + strand at 166.320 kb on + strand at 166.320 kb on + strand at 166.320 kb on + strand at 166.320 kb on + strand at 166.321 kb on - strand at 166.321 kb on - strand at 166.321 kb on - strand at 166.321 kb on - strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.324 kb on + strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.325 kb on - strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.335 kb on + strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.336 kb on - strand at 166.419 kb on - strand at 166.423 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.497 kb on + strand at 166.498 kb on - strand at 166.498 kb on - strand at 166.527 kb on + strand at 166.527 kb on + strand at 166.528 kb on - strand at 166.557 kb on - strand at 166.629 kb on - strand at 166.629 kb on - strand at 166.654 kb on - strand at 166.655 kb on + strand at 166.682 kb on + strand at 166.682 kb on + strand at 166.683 kb on - strand at 166.751 kb on + strand, within COO64_RS11755 at 166.751 kb on + strand, within COO64_RS11755 at 166.752 kb on - strand, within COO64_RS11755 at 166.752 kb on - strand, within COO64_RS11755 at 166.941 kb on + strand, within COO64_RS11755 at 166.942 kb on - strand, within COO64_RS11755 at 166.973 kb on + strand, within COO64_RS11755 at 166.974 kb on - strand, within COO64_RS11755 at 166.974 kb on - strand, within COO64_RS11755 at 167.107 kb on + strand at 167.107 kb on + strand at 167.107 kb on + strand at 167.107 kb on + strand at 167.107 kb on + strand at 167.108 kb on - strand at 167.108 kb on - strand at 167.108 kb on - strand at 167.108 kb on - strand at 167.108 kb on - strand at 167.108 kb on - strand at 167.137 kb on + strand at 167.137 kb on + strand at 167.137 kb on + strand at 167.137 kb on + strand at 167.137 kb on + strand at 167.138 kb on - strand at 167.138 kb on - strand at 167.138 kb on - strand at 167.138 kb on - strand at 167.138 kb on - strand at 167.139 kb on + strand at 167.154 kb on + strand at 167.154 kb on + strand at 167.154 kb on + strand at 167.155 kb on - strand at 167.180 kb on + strand at 167.180 kb on + strand at 167.180 kb on + strand at 167.180 kb on + strand at 167.181 kb on - strand at 167.182 kb on + strand at 167.182 kb on + strand at 167.201 kb on + strand at 167.201 kb on + strand at 167.201 kb on + strand at 167.202 kb on - strand at 167.224 kb on + strand at 167.225 kb on - strand at 167.225 kb on - strand at 167.225 kb on - strand at 167.225 kb on - strand at 167.285 kb on + strand at 167.285 kb on + strand at 167.285 kb on + strand at 167.285 kb on + strand at 167.286 kb on - strand at 167.286 kb on - strand at 167.286 kb on - strand at 167.323 kb on + strand at 167.323 kb on + strand at 167.324 kb on - strand at 167.324 kb on - strand at 167.324 kb on - strand at 167.444 kb on - strand, within COO64_RS11760 at 167.465 kb on - strand, within COO64_RS11760 at 167.537 kb on + strand, within COO64_RS11760 at 167.538 kb on - strand, within COO64_RS11760 at 167.698 kb on - strand, within COO64_RS11760 at 167.698 kb on - strand, within COO64_RS11760 at 167.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 166,287 + -0.4 166,287 + +2.0 166,288 - -0.3 166,288 - -0.4 166,288 - -0.2 166,288 - -1.1 166,288 - +0.6 166,288 - +0.0 166,288 - -0.4 166,320 + +0.6 166,320 + +0.8 166,320 + -0.2 166,320 + +0.3 166,320 + -0.7 166,320 + -0.5 166,321 - +0.2 166,321 - -1.0 166,321 - +1.0 166,321 - -0.2 166,324 + +0.1 166,324 + -0.2 166,324 + +0.5 166,324 + +0.3 166,324 + -0.0 166,324 + -0.2 166,324 + +0.1 166,324 + +0.8 166,324 + -0.3 166,324 + +1.7 166,324 + -1.0 166,324 + +1.9 166,324 + -0.6 166,324 + +1.0 166,324 + +0.8 166,325 - +0.3 166,325 - +0.5 166,325 - -0.2 166,325 - +0.6 166,325 - +0.6 166,325 - +0.5 166,325 - -0.4 166,325 - -0.5 166,325 - -0.1 166,325 - -0.4 166,325 - +0.9 166,335 + -0.4 166,335 + -0.7 166,335 + -0.1 166,335 + +0.2 166,335 + +0.5 166,335 + -0.3 166,335 + -1.2 166,335 + +0.3 166,335 + +0.5 166,335 + -0.4 166,335 + -0.0 166,336 - -0.4 166,336 - +1.4 166,336 - +0.5 166,336 - +1.2 166,336 - +0.1 166,336 - +0.5 166,336 - +0.5 166,336 - -0.5 166,336 - -0.0 166,336 - -0.0 166,336 - +0.0 166,336 - +0.7 166,336 - -0.1 166,336 - -0.6 166,336 - -1.0 166,336 - +0.5 166,419 - -1.3 166,423 + -0.8 166,497 + +0.0 166,497 + -1.3 166,497 + -0.5 166,497 + -0.1 166,497 + -0.6 166,497 + -0.0 166,497 + -0.9 166,498 - -0.7 166,498 - -0.2 166,527 + -0.2 166,527 + +0.8 166,528 - +1.0 166,557 - -0.0 166,629 - +1.3 166,629 - +0.2 166,654 - +0.3 166,655 + +0.5 166,682 + -0.5 166,682 + -0.2 166,683 - -1.0 166,751 + COO64_RS11755 0.14 -0.5 166,751 + COO64_RS11755 0.14 -0.4 166,752 - COO64_RS11755 0.14 -0.1 166,752 - COO64_RS11755 0.14 -0.2 166,941 + COO64_RS11755 0.58 -0.9 166,942 - COO64_RS11755 0.58 +0.0 166,973 + COO64_RS11755 0.65 -0.3 166,974 - COO64_RS11755 0.65 +0.3 166,974 - COO64_RS11755 0.65 -0.3 167,107 + +0.1 167,107 + +1.7 167,107 + +0.3 167,107 + -0.4 167,107 + +0.5 167,108 - -0.5 167,108 - -1.6 167,108 - +0.1 167,108 - +0.1 167,108 - -0.2 167,108 - -0.2 167,137 + +0.2 167,137 + +0.0 167,137 + +0.0 167,137 + -0.7 167,137 + -0.1 167,138 - +0.1 167,138 - -1.0 167,138 - +0.0 167,138 - +0.1 167,138 - -2.0 167,139 + +0.0 167,154 + +0.1 167,154 + +0.0 167,154 + -0.1 167,155 - -0.4 167,180 + -0.3 167,180 + -0.4 167,180 + +0.2 167,180 + -0.1 167,181 - -0.5 167,182 + -0.6 167,182 + +0.2 167,201 + +1.5 167,201 + +0.4 167,201 + +1.4 167,202 - -0.5 167,224 + -0.0 167,225 - -0.5 167,225 - -0.5 167,225 - -0.9 167,225 - +1.0 167,285 + -1.0 167,285 + +0.6 167,285 + -1.0 167,285 + -0.3 167,286 - +0.1 167,286 - -0.1 167,286 - +0.8 167,323 + +0.5 167,323 + -1.0 167,324 - +0.5 167,324 - +0.5 167,324 - +0.3 167,444 - COO64_RS11760 0.29 -1.0 167,465 - COO64_RS11760 0.32 +0.3 167,537 + COO64_RS11760 0.43 -1.2 167,538 - COO64_RS11760 0.43 +0.5 167,698 - COO64_RS11760 0.69 +0.2 167,698 - COO64_RS11760 0.69 -0.8 167,896 + +1.0
Or see this region's nucleotide sequence