Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS11140 and COO64_RS11145 are separated by 218 nucleotides COO64_RS11145 and COO64_RS11150 are separated by 70 nucleotides COO64_RS11150 and COO64_RS11155 are separated by 161 nucleotides
COO64_RS11140: COO64_RS11140 - type II secretion system F family protein, at 29,556 to 30,764
_RS11140
COO64_RS11145: COO64_RS11145 - pilin, at 30,983 to 31,393
_RS11145
COO64_RS11150: COO64_RS11150 - tRNA-Thr, at 31,464 to 31,539
_RS11150
COO64_RS11155: COO64_RS11155 - phage integrase central domain-containing protein, at 31,701 to 33,017
_RS11155
Position (kb)
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 30.630 kb on - strand, within COO64_RS11140 at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.742 kb on + strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.743 kb on - strand at 30.849 kb on + strand at 30.849 kb on + strand at 30.850 kb on - strand at 30.854 kb on + strand at 30.854 kb on + strand at 30.854 kb on + strand at 30.854 kb on + strand at 30.854 kb on + strand at 30.854 kb on + strand at 30.855 kb on - strand at 30.855 kb on - strand at 30.914 kb on + strand at 30.914 kb on + strand at 31.001 kb on - strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.003 kb on + strand at 31.004 kb on - strand at 31.004 kb on - strand at 31.070 kb on + strand, within COO64_RS11145 at 31.086 kb on + strand, within COO64_RS11145 at 31.086 kb on + strand, within COO64_RS11145 at 31.087 kb on - strand, within COO64_RS11145 at 31.415 kb on + strand at 31.415 kb on + strand at 31.416 kb on - strand at 31.416 kb on - strand at 31.416 kb on - strand at 31.416 kb on - strand at 31.471 kb on + strand at 31.472 kb on - strand, within COO64_RS11150 at 31.472 kb on - strand, within COO64_RS11150 at 31.550 kb on + strand at 31.596 kb on - strand at 31.672 kb on - strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.767 kb on + strand at 31.768 kb on - strand at 31.768 kb on - strand at 31.768 kb on - strand at 31.768 kb on - strand at 31.768 kb on - strand at 31.794 kb on + strand at 31.794 kb on + strand at 31.794 kb on + strand at 31.794 kb on + strand at 31.795 kb on - strand at 31.839 kb on + strand, within COO64_RS11155 at 31.839 kb on + strand, within COO64_RS11155 at 31.839 kb on + strand, within COO64_RS11155 at 31.839 kb on + strand, within COO64_RS11155 at 31.840 kb on - strand, within COO64_RS11155 at 31.840 kb on - strand, within COO64_RS11155 at 31.840 kb on - strand, within COO64_RS11155 at 31.840 kb on - strand, within COO64_RS11155 at 31.961 kb on + strand, within COO64_RS11155 at 31.962 kb on - strand, within COO64_RS11155 at 31.962 kb on - strand, within COO64_RS11155 at 32.012 kb on + strand, within COO64_RS11155 at 32.012 kb on + strand, within COO64_RS11155 at 32.013 kb on - strand, within COO64_RS11155 at 32.057 kb on + strand, within COO64_RS11155 at 32.057 kb on + strand, within COO64_RS11155 at 32.058 kb on - strand, within COO64_RS11155 at 32.058 kb on - strand, within COO64_RS11155 at 32.113 kb on + strand, within COO64_RS11155 at 32.114 kb on - strand, within COO64_RS11155 at 32.114 kb on - strand, within COO64_RS11155 at 32.146 kb on + strand, within COO64_RS11155 at 32.146 kb on + strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.147 kb on - strand, within COO64_RS11155 at 32.167 kb on + strand, within COO64_RS11155 at 32.167 kb on + strand, within COO64_RS11155 at 32.167 kb on + strand, within COO64_RS11155 at 32.168 kb on - strand, within COO64_RS11155 at 32.168 kb on - strand, within COO64_RS11155 at 32.168 kb on - strand, within COO64_RS11155 at 32.226 kb on + strand, within COO64_RS11155 at 32.226 kb on + strand, within COO64_RS11155 at 32.226 kb on + strand, within COO64_RS11155 at 32.226 kb on + strand, within COO64_RS11155 at 32.226 kb on + strand, within COO64_RS11155 at 32.227 kb on - strand, within COO64_RS11155 at 32.227 kb on - strand, within COO64_RS11155 at 32.227 kb on - strand, within COO64_RS11155 at 32.227 kb on - strand, within COO64_RS11155 at 32.269 kb on + strand, within COO64_RS11155 at 32.269 kb on + strand, within COO64_RS11155 at 32.269 kb on + strand, within COO64_RS11155 at 32.269 kb on + strand, within COO64_RS11155 at 32.269 kb on + strand, within COO64_RS11155 at 32.270 kb on - strand, within COO64_RS11155 at 32.270 kb on - strand, within COO64_RS11155 at 32.402 kb on + strand, within COO64_RS11155 at 32.402 kb on + strand, within COO64_RS11155 at 32.402 kb on + strand, within COO64_RS11155 at 32.403 kb on - strand, within COO64_RS11155 at 32.403 kb on - strand, within COO64_RS11155 at 32.403 kb on - strand, within COO64_RS11155 at 32.403 kb on - strand, within COO64_RS11155 at 32.403 kb on - strand, within COO64_RS11155 at 32.491 kb on + strand, within COO64_RS11155 at 32.492 kb on - strand, within COO64_RS11155 at 32.533 kb on + strand, within COO64_RS11155 at 32.534 kb on - strand, within COO64_RS11155
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 30,630 - COO64_RS11140 0.89 +0.7 30,742 + -0.6 30,742 + -1.4 30,742 + -1.7 30,742 + +0.3 30,742 + +0.3 30,742 + -1.1 30,742 + +0.0 30,742 + +0.6 30,742 + +0.5 30,742 + +0.4 30,743 - -0.2 30,743 - -0.3 30,743 - +0.1 30,743 - +1.2 30,743 - -0.2 30,743 - -0.5 30,743 - +0.3 30,743 - +0.3 30,849 + -0.5 30,849 + -0.1 30,850 - -0.3 30,854 + +0.1 30,854 + -0.8 30,854 + -0.8 30,854 + +0.6 30,854 + +0.9 30,854 + +0.4 30,855 - +0.7 30,855 - -0.5 30,914 + -0.9 30,914 + -0.6 31,001 - +0.3 31,003 + +0.7 31,003 + +0.6 31,003 + +0.6 31,003 + +0.3 31,003 + +0.5 31,003 + -0.7 31,003 + -0.3 31,003 + +0.9 31,003 + +0.0 31,003 + -0.4 31,003 + -1.0 31,004 - +0.6 31,004 - +0.8 31,070 + COO64_RS11145 0.21 -0.2 31,086 + COO64_RS11145 0.25 +0.7 31,086 + COO64_RS11145 0.25 +0.0 31,087 - COO64_RS11145 0.25 -1.6 31,415 + +0.2 31,415 + +0.4 31,416 - +0.4 31,416 - -0.3 31,416 - +0.6 31,416 - -0.1 31,471 + -0.8 31,472 - COO64_RS11150 0.11 -0.8 31,472 - COO64_RS11150 0.11 -1.5 31,550 + -0.8 31,596 - -0.2 31,672 - +0.1 31,767 + -1.9 31,767 + -1.3 31,767 + +0.1 31,767 + -0.6 31,767 + -0.1 31,767 + +0.7 31,767 + +0.6 31,768 - -0.5 31,768 - -1.0 31,768 - -0.2 31,768 - -0.4 31,768 - +0.4 31,794 + -0.6 31,794 + -0.9 31,794 + -0.7 31,794 + +0.1 31,795 - -0.7 31,839 + COO64_RS11155 0.10 -1.3 31,839 + COO64_RS11155 0.10 -1.3 31,839 + COO64_RS11155 0.10 -0.2 31,839 + COO64_RS11155 0.10 -0.3 31,840 - COO64_RS11155 0.11 +0.5 31,840 - COO64_RS11155 0.11 +0.8 31,840 - COO64_RS11155 0.11 -0.3 31,840 - COO64_RS11155 0.11 -0.2 31,961 + COO64_RS11155 0.20 +0.2 31,962 - COO64_RS11155 0.20 -0.2 31,962 - COO64_RS11155 0.20 +0.3 32,012 + COO64_RS11155 0.24 -0.1 32,012 + COO64_RS11155 0.24 +0.0 32,013 - COO64_RS11155 0.24 -0.4 32,057 + COO64_RS11155 0.27 -0.2 32,057 + COO64_RS11155 0.27 -0.1 32,058 - COO64_RS11155 0.27 -2.3 32,058 - COO64_RS11155 0.27 -0.4 32,113 + COO64_RS11155 0.31 +0.3 32,114 - COO64_RS11155 0.31 -0.3 32,114 - COO64_RS11155 0.31 -0.5 32,146 + COO64_RS11155 0.34 -0.2 32,146 + COO64_RS11155 0.34 -0.2 32,147 - COO64_RS11155 0.34 +0.3 32,147 - COO64_RS11155 0.34 -0.5 32,147 - COO64_RS11155 0.34 +0.4 32,147 - COO64_RS11155 0.34 -0.1 32,147 - COO64_RS11155 0.34 -0.8 32,147 - COO64_RS11155 0.34 -0.3 32,147 - COO64_RS11155 0.34 -1.3 32,147 - COO64_RS11155 0.34 +0.1 32,167 + COO64_RS11155 0.35 +1.5 32,167 + COO64_RS11155 0.35 +1.6 32,167 + COO64_RS11155 0.35 -0.3 32,168 - COO64_RS11155 0.35 -0.5 32,168 - COO64_RS11155 0.35 -0.8 32,168 - COO64_RS11155 0.35 +0.6 32,226 + COO64_RS11155 0.40 -0.4 32,226 + COO64_RS11155 0.40 -0.1 32,226 + COO64_RS11155 0.40 -1.0 32,226 + COO64_RS11155 0.40 -0.2 32,226 + COO64_RS11155 0.40 +0.1 32,227 - COO64_RS11155 0.40 -0.4 32,227 - COO64_RS11155 0.40 -0.7 32,227 - COO64_RS11155 0.40 +0.2 32,227 - COO64_RS11155 0.40 -1.8 32,269 + COO64_RS11155 0.43 -0.6 32,269 + COO64_RS11155 0.43 -0.9 32,269 + COO64_RS11155 0.43 +0.0 32,269 + COO64_RS11155 0.43 -0.7 32,269 + COO64_RS11155 0.43 +0.6 32,270 - COO64_RS11155 0.43 +0.7 32,270 - COO64_RS11155 0.43 +0.2 32,402 + COO64_RS11155 0.53 +0.9 32,402 + COO64_RS11155 0.53 -0.1 32,402 + COO64_RS11155 0.53 -0.6 32,403 - COO64_RS11155 0.53 +0.2 32,403 - COO64_RS11155 0.53 +0.5 32,403 - COO64_RS11155 0.53 -0.6 32,403 - COO64_RS11155 0.53 +0.2 32,403 - COO64_RS11155 0.53 +0.0 32,491 + COO64_RS11155 0.60 +0.2 32,492 - COO64_RS11155 0.60 -0.5 32,533 + COO64_RS11155 0.63 -0.7 32,534 - COO64_RS11155 0.63 +0.5
Or see this region's nucleotide sequence