Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS10340 and rbsK are separated by 14 nucleotides rbsK and rbsD overlap by 4 nucleotides rbsD and COO64_RS10355 are separated by 74 nucleotides
COO64_RS10340: COO64_RS10340 - LacI family DNA-binding transcriptional regulator, at 220,231 to 221,253
_RS10340
COO64_RS10345: rbsK - ribokinase, at 221,268 to 222,176
rbsK
COO64_RS10350: rbsD - D-ribose pyranase, at 222,173 to 222,577
rbsD
COO64_RS10355: COO64_RS10355 - nucleoside hydrolase, at 222,652 to 223,623
_RS10355
Position (kb) 222
223 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 221.281 kb on + strand at 221.281 kb on + strand at 221.281 kb on + strand at 221.281 kb on + strand at 221.281 kb on + strand at 221.281 kb on + strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.282 kb on - strand at 221.284 kb on + strand at 221.285 kb on - strand at 221.285 kb on - strand at 221.285 kb on - strand at 221.290 kb on + strand at 221.290 kb on + strand at 221.290 kb on + strand at 221.290 kb on + strand at 221.290 kb on + strand at 221.290 kb on + strand at 221.291 kb on - strand at 221.291 kb on - strand at 221.291 kb on - strand at 221.291 kb on - strand at 221.291 kb on - strand at 221.294 kb on + strand at 221.467 kb on - strand, within rbsK at 221.610 kb on - strand, within rbsK at 221.712 kb on + strand, within rbsK at 221.712 kb on + strand, within rbsK at 221.712 kb on + strand, within rbsK at 221.811 kb on + strand, within rbsK at 221.811 kb on + strand, within rbsK at 221.811 kb on + strand, within rbsK at 221.811 kb on + strand, within rbsK at 221.812 kb on - strand, within rbsK at 221.812 kb on - strand, within rbsK at 222.517 kb on - strand, within rbsD at 222.517 kb on - strand, within rbsD at 222.517 kb on - strand, within rbsD at 222.517 kb on - strand, within rbsD at 222.539 kb on + strand at 222.539 kb on + strand at 222.540 kb on - strand at 222.540 kb on - strand at 222.576 kb on - strand at 222.597 kb on - strand at 222.609 kb on + strand at 222.609 kb on + strand at 222.609 kb on + strand at 222.609 kb on + strand at 222.609 kb on + strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.610 kb on - strand at 222.644 kb on + strand at 222.644 kb on + strand at 222.650 kb on + strand at 222.650 kb on + strand at 222.650 kb on + strand at 222.650 kb on + strand at 222.700 kb on - strand at 222.705 kb on + strand at 222.874 kb on + strand, within COO64_RS10355 at 222.875 kb on - strand, within COO64_RS10355 at 222.875 kb on - strand, within COO64_RS10355 at 222.913 kb on + strand, within COO64_RS10355 at 222.913 kb on + strand, within COO64_RS10355 at 222.914 kb on - strand, within COO64_RS10355 at 223.003 kb on + strand, within COO64_RS10355 at 223.164 kb on + strand, within COO64_RS10355 at 223.164 kb on + strand, within COO64_RS10355 at 223.246 kb on + strand, within COO64_RS10355 at 223.246 kb on + strand, within COO64_RS10355 at 223.246 kb on + strand, within COO64_RS10355 at 223.247 kb on - strand, within COO64_RS10355 at 223.247 kb on - strand, within COO64_RS10355 at 223.247 kb on - strand, within COO64_RS10355 at 223.247 kb on - strand, within COO64_RS10355 at 223.382 kb on - strand, within COO64_RS10355 at 223.418 kb on + strand, within COO64_RS10355 at 223.426 kb on + strand, within COO64_RS10355 at 223.434 kb on + strand, within COO64_RS10355 at 223.434 kb on + strand, within COO64_RS10355 at 223.434 kb on + strand, within COO64_RS10355 at 223.434 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.463 kb on + strand, within COO64_RS10355 at 223.464 kb on - strand, within COO64_RS10355 at 223.464 kb on - strand, within COO64_RS10355 at 223.464 kb on - strand, within COO64_RS10355
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 221,281 + -0.8 221,281 + -0.8 221,281 + +0.2 221,281 + -0.7 221,281 + -0.5 221,281 + -0.4 221,282 - -0.6 221,282 - +0.7 221,282 - -0.4 221,282 - -0.7 221,282 - -0.5 221,282 - -0.4 221,282 - -0.4 221,282 - -0.9 221,282 - +0.7 221,282 - +0.2 221,284 + +0.3 221,285 - -0.7 221,285 - -0.2 221,285 - +1.0 221,290 + +0.3 221,290 + -0.3 221,290 + -0.5 221,290 + -2.1 221,290 + -0.2 221,290 + -0.6 221,291 - +0.1 221,291 - +0.4 221,291 - -1.1 221,291 - -0.8 221,291 - +0.9 221,294 + +0.2 221,467 - rbsK COO64_RS10345 0.22 +0.7 221,610 - rbsK COO64_RS10345 0.38 -0.2 221,712 + rbsK COO64_RS10345 0.49 -0.1 221,712 + rbsK COO64_RS10345 0.49 -0.6 221,712 + rbsK COO64_RS10345 0.49 -0.6 221,811 + rbsK COO64_RS10345 0.60 +0.6 221,811 + rbsK COO64_RS10345 0.60 +0.3 221,811 + rbsK COO64_RS10345 0.60 -0.3 221,811 + rbsK COO64_RS10345 0.60 -0.8 221,812 - rbsK COO64_RS10345 0.60 -0.7 221,812 - rbsK COO64_RS10345 0.60 +0.3 222,517 - rbsD COO64_RS10350 0.85 -1.3 222,517 - rbsD COO64_RS10350 0.85 -0.1 222,517 - rbsD COO64_RS10350 0.85 -0.7 222,517 - rbsD COO64_RS10350 0.85 -0.9 222,539 + -0.0 222,539 + -0.2 222,540 - +0.5 222,540 - +0.7 222,576 - -0.1 222,597 - +0.7 222,609 + +0.1 222,609 + +0.0 222,609 + +0.8 222,609 + +1.2 222,609 + +0.1 222,610 - -1.1 222,610 - +0.0 222,610 - +0.8 222,610 - -0.9 222,610 - +0.9 222,610 - -0.3 222,610 - -0.3 222,610 - +0.2 222,644 + -0.2 222,644 + -0.3 222,650 + -0.7 222,650 + +0.8 222,650 + -0.0 222,650 + -0.0 222,700 - +0.1 222,705 + -0.4 222,874 + COO64_RS10355 0.23 +0.3 222,875 - COO64_RS10355 0.23 -1.0 222,875 - COO64_RS10355 0.23 -2.4 222,913 + COO64_RS10355 0.27 +0.2 222,913 + COO64_RS10355 0.27 -0.7 222,914 - COO64_RS10355 0.27 +0.6 223,003 + COO64_RS10355 0.36 +0.5 223,164 + COO64_RS10355 0.53 +0.7 223,164 + COO64_RS10355 0.53 +0.9 223,246 + COO64_RS10355 0.61 +1.0 223,246 + COO64_RS10355 0.61 +0.5 223,246 + COO64_RS10355 0.61 -0.0 223,247 - COO64_RS10355 0.61 -0.9 223,247 - COO64_RS10355 0.61 +0.4 223,247 - COO64_RS10355 0.61 -0.5 223,247 - COO64_RS10355 0.61 -0.2 223,382 - COO64_RS10355 0.75 +0.2 223,418 + COO64_RS10355 0.79 +0.1 223,426 + COO64_RS10355 0.80 -0.6 223,434 + COO64_RS10355 0.80 +0.1 223,434 + COO64_RS10355 0.80 +0.3 223,434 + COO64_RS10355 0.80 +0.2 223,434 + COO64_RS10355 0.80 -0.5 223,463 + COO64_RS10355 0.83 -1.1 223,463 + COO64_RS10355 0.83 -0.4 223,463 + COO64_RS10355 0.83 -0.0 223,463 + COO64_RS10355 0.83 +0.6 223,463 + COO64_RS10355 0.83 -1.1 223,463 + COO64_RS10355 0.83 -2.8 223,464 - COO64_RS10355 0.84 -1.1 223,464 - COO64_RS10355 0.84 +0.5 223,464 - COO64_RS10355 0.84 -0.3
Or see this region's nucleotide sequence