Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS09820 and COO64_RS09825 are separated by 92 nucleotides COO64_RS09825 and cysB are separated by 124 nucleotides cysB and COO64_RS09835 are separated by 239 nucleotides COO64_RS09835 and COO64_RS09840 are separated by 162 nucleotides
COO64_RS09820: COO64_RS09820 - 5'-nucleotidase, at 95,096 to 96,004
_RS09820
COO64_RS09825: COO64_RS09825 - universal stress protein, at 96,097 to 96,594
_RS09825
COO64_RS09830: cysB - HTH-type transcriptional regulator CysB, at 96,719 to 97,693
cysB
COO64_RS09835: COO64_RS09835 - CS1 type fimbrial major subunit, at 97,933 to 98,412
_RS09835
COO64_RS09840: COO64_RS09840 - CS1-pili formation C-terminal domain-containing protein, at 98,575 to 101,040
_RS09840
Position (kb)
96
97
98 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 96.053 kb on + strand at 96.053 kb on + strand at 96.054 kb on - strand at 96.054 kb on - strand at 96.054 kb on - strand at 96.054 kb on - strand at 96.115 kb on + strand at 96.115 kb on + strand at 96.115 kb on + strand at 96.115 kb on + strand at 96.115 kb on + strand at 96.115 kb on + strand at 96.116 kb on - strand at 96.116 kb on - strand at 96.136 kb on + strand at 96.137 kb on - strand at 96.196 kb on + strand, within COO64_RS09825 at 96.196 kb on + strand, within COO64_RS09825 at 96.196 kb on + strand, within COO64_RS09825 at 96.197 kb on - strand, within COO64_RS09825 at 96.197 kb on - strand, within COO64_RS09825 at 96.197 kb on - strand, within COO64_RS09825 at 96.197 kb on - strand, within COO64_RS09825 at 96.254 kb on - strand, within COO64_RS09825 at 96.254 kb on - strand, within COO64_RS09825 at 96.474 kb on + strand, within COO64_RS09825 at 96.550 kb on + strand at 96.550 kb on + strand at 96.550 kb on + strand at 96.550 kb on + strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.551 kb on - strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.552 kb on + strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.553 kb on - strand at 96.582 kb on + strand at 96.582 kb on + strand at 96.592 kb on + strand at 96.593 kb on - strand at 96.593 kb on - strand at 96.609 kb on + strand at 96.609 kb on + strand at 96.609 kb on + strand at 96.609 kb on + strand at 96.610 kb on - strand at 96.610 kb on - strand at 96.610 kb on - strand at 96.610 kb on - strand at 96.633 kb on + strand at 96.663 kb on + strand at 96.664 kb on - strand at 96.669 kb on + strand at 96.714 kb on + strand at 96.715 kb on - strand at 96.715 kb on - strand at 96.715 kb on - strand at 96.715 kb on - strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.718 kb on + strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.719 kb on - strand at 96.740 kb on + strand at 96.740 kb on + strand at 96.741 kb on - strand at 96.797 kb on + strand at 96.797 kb on + strand at 96.797 kb on + strand at 96.798 kb on - strand at 96.798 kb on - strand at 96.799 kb on + strand at 96.799 kb on + strand at 96.799 kb on + strand at 96.799 kb on + strand at 96.799 kb on + strand at 96.799 kb on + strand at 96.800 kb on - strand at 96.800 kb on - strand at 96.800 kb on - strand at 96.800 kb on - strand at 96.800 kb on - strand at 96.800 kb on - strand at 96.820 kb on + strand, within cysB at 96.820 kb on + strand, within cysB at 96.820 kb on + strand, within cysB at 96.821 kb on - strand, within cysB at 97.034 kb on + strand, within cysB at 97.034 kb on + strand, within cysB at 97.035 kb on - strand, within cysB at 97.035 kb on - strand, within cysB at 97.307 kb on + strand, within cysB at 97.307 kb on + strand, within cysB at 97.307 kb on + strand, within cysB at 97.307 kb on + strand, within cysB at 97.307 kb on + strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.308 kb on - strand, within cysB at 97.380 kb on + strand, within cysB at 97.381 kb on - strand, within cysB at 97.415 kb on + strand, within cysB at 97.415 kb on + strand, within cysB at 97.415 kb on + strand, within cysB at 97.415 kb on + strand, within cysB at 97.416 kb on - strand, within cysB at 97.416 kb on - strand, within cysB at 97.416 kb on - strand, within cysB at 97.743 kb on + strand at 97.759 kb on + strand at 97.759 kb on + strand at 97.759 kb on + strand at 97.760 kb on - strand at 97.760 kb on - strand at 97.760 kb on - strand at 97.826 kb on + strand at 98.022 kb on + strand, within COO64_RS09835 at 98.023 kb on - strand, within COO64_RS09835 at 98.023 kb on - strand, within COO64_RS09835 at 98.023 kb on - strand, within COO64_RS09835 at 98.024 kb on + strand, within COO64_RS09835 at 98.024 kb on + strand, within COO64_RS09835 at 98.024 kb on + strand, within COO64_RS09835 at 98.025 kb on - strand, within COO64_RS09835 at 98.025 kb on - strand, within COO64_RS09835 at 98.025 kb on - strand, within COO64_RS09835 at 98.025 kb on - strand, within COO64_RS09835 at 98.032 kb on - strand, within COO64_RS09835 at 98.051 kb on - strand, within COO64_RS09835 at 98.051 kb on - strand, within COO64_RS09835 at 98.234 kb on + strand, within COO64_RS09835 at 98.234 kb on + strand, within COO64_RS09835 at 98.353 kb on + strand, within COO64_RS09835 at 98.368 kb on + strand at 98.369 kb on - strand at 98.369 kb on - strand at 98.369 kb on - strand at 98.410 kb on + strand at 98.410 kb on + strand at 98.488 kb on - strand at 98.518 kb on + strand at 98.518 kb on + strand at 98.519 kb on - strand at 98.528 kb on + strand at 98.528 kb on + strand at 98.529 kb on - strand at 98.529 kb on - strand at 98.624 kb on + strand at 98.624 kb on + strand at 98.624 kb on + strand at 98.624 kb on + strand at 98.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 96,053 + +0.2 96,053 + -0.8 96,054 - -0.8 96,054 - +0.6 96,054 - -0.9 96,054 - -0.1 96,115 + +0.8 96,115 + +0.5 96,115 + +0.7 96,115 + -0.9 96,115 + +0.3 96,115 + -0.7 96,116 - +1.2 96,116 - -0.2 96,136 + +1.3 96,137 - +0.3 96,196 + COO64_RS09825 0.20 -0.1 96,196 + COO64_RS09825 0.20 +1.0 96,196 + COO64_RS09825 0.20 +1.4 96,197 - COO64_RS09825 0.20 -0.0 96,197 - COO64_RS09825 0.20 -1.1 96,197 - COO64_RS09825 0.20 -0.8 96,197 - COO64_RS09825 0.20 -0.7 96,254 - COO64_RS09825 0.32 +1.6 96,254 - COO64_RS09825 0.32 -0.6 96,474 + COO64_RS09825 0.76 +0.4 96,550 + +0.1 96,550 + +0.4 96,550 + -0.4 96,550 + -2.2 96,551 - -0.1 96,551 - -0.3 96,551 - -0.8 96,551 - +0.3 96,551 - +0.3 96,551 - +0.9 96,551 - -0.5 96,551 - +0.6 96,551 - -1.0 96,552 + +0.5 96,552 + +0.5 96,552 + +0.2 96,552 + -0.9 96,552 + +0.7 96,552 + -1.1 96,552 + +0.5 96,552 + +0.1 96,553 - -1.2 96,553 - +0.3 96,553 - +0.3 96,553 - +0.2 96,553 - +0.9 96,553 - -1.4 96,553 - -0.2 96,582 + +0.3 96,582 + +0.1 96,592 + +0.1 96,593 - +0.3 96,593 - -1.3 96,609 + +0.7 96,609 + +0.0 96,609 + +0.2 96,609 + +0.9 96,610 - +0.6 96,610 - -0.2 96,610 - +0.2 96,610 - -0.8 96,633 + +0.6 96,663 + -0.4 96,664 - -0.4 96,669 + +0.4 96,714 + -0.6 96,715 - -0.8 96,715 - -1.0 96,715 - -0.1 96,715 - +1.1 96,718 + +0.2 96,718 + -1.2 96,718 + +0.3 96,718 + -1.2 96,718 + -0.3 96,718 + +0.8 96,718 + -0.1 96,718 + +0.4 96,718 + -0.1 96,718 + +0.5 96,718 + -2.0 96,719 - -0.6 96,719 - +0.4 96,719 - -0.3 96,719 - -0.5 96,719 - +0.9 96,719 - -1.7 96,719 - +0.4 96,719 - -1.1 96,719 - -0.6 96,719 - +0.3 96,719 - -0.9 96,740 + -1.5 96,740 + -0.3 96,741 - +0.6 96,797 + +0.2 96,797 + -0.7 96,797 + -0.8 96,798 - -1.9 96,798 - -0.2 96,799 + -0.5 96,799 + -0.1 96,799 + -0.4 96,799 + +0.3 96,799 + -0.0 96,799 + -0.5 96,800 - +0.4 96,800 - -0.4 96,800 - -0.1 96,800 - -0.6 96,800 - +0.3 96,800 - -0.5 96,820 + cysB COO64_RS09830 0.10 -1.4 96,820 + cysB COO64_RS09830 0.10 -2.5 96,820 + cysB COO64_RS09830 0.10 +0.0 96,821 - cysB COO64_RS09830 0.10 +0.5 97,034 + cysB COO64_RS09830 0.32 +0.6 97,034 + cysB COO64_RS09830 0.32 -0.0 97,035 - cysB COO64_RS09830 0.32 +0.0 97,035 - cysB COO64_RS09830 0.32 +0.0 97,307 + cysB COO64_RS09830 0.60 -0.4 97,307 + cysB COO64_RS09830 0.60 -0.5 97,307 + cysB COO64_RS09830 0.60 -0.5 97,307 + cysB COO64_RS09830 0.60 -1.1 97,307 + cysB COO64_RS09830 0.60 -0.9 97,308 - cysB COO64_RS09830 0.60 -2.1 97,308 - cysB COO64_RS09830 0.60 -0.1 97,308 - cysB COO64_RS09830 0.60 -1.5 97,308 - cysB COO64_RS09830 0.60 -0.9 97,308 - cysB COO64_RS09830 0.60 -0.2 97,308 - cysB COO64_RS09830 0.60 -2.3 97,308 - cysB COO64_RS09830 0.60 -1.0 97,380 + cysB COO64_RS09830 0.68 -0.5 97,381 - cysB COO64_RS09830 0.68 +0.8 97,415 + cysB COO64_RS09830 0.71 -2.3 97,415 + cysB COO64_RS09830 0.71 -1.1 97,415 + cysB COO64_RS09830 0.71 -0.9 97,415 + cysB COO64_RS09830 0.71 -0.9 97,416 - cysB COO64_RS09830 0.71 +0.1 97,416 - cysB COO64_RS09830 0.71 +0.2 97,416 - cysB COO64_RS09830 0.71 -0.2 97,743 + +0.5 97,759 + -0.1 97,759 + -0.3 97,759 + -0.3 97,760 - -0.7 97,760 - +1.9 97,760 - +0.3 97,826 + +0.1 98,022 + COO64_RS09835 0.19 +0.4 98,023 - COO64_RS09835 0.19 -1.0 98,023 - COO64_RS09835 0.19 +0.5 98,023 - COO64_RS09835 0.19 +0.2 98,024 + COO64_RS09835 0.19 -0.1 98,024 + COO64_RS09835 0.19 -0.8 98,024 + COO64_RS09835 0.19 +0.0 98,025 - COO64_RS09835 0.19 -0.4 98,025 - COO64_RS09835 0.19 +0.1 98,025 - COO64_RS09835 0.19 -0.6 98,025 - COO64_RS09835 0.19 +0.3 98,032 - COO64_RS09835 0.21 +0.2 98,051 - COO64_RS09835 0.25 -1.0 98,051 - COO64_RS09835 0.25 -1.8 98,234 + COO64_RS09835 0.63 +0.8 98,234 + COO64_RS09835 0.63 +0.4 98,353 + COO64_RS09835 0.88 -0.0 98,368 + +0.2 98,369 - +0.3 98,369 - +0.5 98,369 - -0.9 98,410 + +0.3 98,410 + +0.2 98,488 - +1.2 98,518 + +0.2 98,518 + +0.5 98,519 - -0.4 98,528 + +0.5 98,528 + +0.1 98,529 - +0.6 98,529 - -1.2 98,624 + -0.1 98,624 + +0.7 98,624 + -0.1 98,624 + -0.8 98,624 + -0.0
Or see this region's nucleotide sequence