Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fadA and COO64_RS09405 are separated by 79 nucleotides COO64_RS09405 and topA are separated by 100 nucleotides topA and COO64_RS09415 are separated by 140 nucleotides COO64_RS09415 and COO64_RS09420 are separated by 50 nucleotides
COO64_RS09400: fadA - acetyl-CoA C-acyltransferase FadA, at 8,977 to 10,152
fadA
COO64_RS09405: COO64_RS09405 - DUF1653 domain-containing protein, at 10,232 to 10,483
_RS09405
COO64_RS09410: topA - type I DNA topoisomerase, at 10,584 to 13,196
topA
COO64_RS09415: COO64_RS09415 - DUF6586 family protein, at 13,337 to 13,858
_RS09415
COO64_RS09420: COO64_RS09420 - hypothetical protein, at 13,909 to 14,142
_RS09420
Position (kb)
10
11
12
13
14 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 9.681 kb on + strand, within fadA at 10.239 kb on + strand at 10.240 kb on - strand at 10.240 kb on - strand at 10.240 kb on - strand at 10.240 kb on - strand at 10.240 kb on - strand at 10.251 kb on + strand at 10.252 kb on - strand at 10.252 kb on - strand at 10.252 kb on - strand at 10.252 kb on - strand at 10.254 kb on - strand at 10.262 kb on + strand, within COO64_RS09405 at 10.262 kb on + strand, within COO64_RS09405 at 10.262 kb on + strand, within COO64_RS09405 at 10.263 kb on - strand, within COO64_RS09405 at 10.263 kb on - strand, within COO64_RS09405 at 10.263 kb on - strand, within COO64_RS09405 at 10.277 kb on + strand, within COO64_RS09405 at 10.278 kb on - strand, within COO64_RS09405 at 10.331 kb on + strand, within COO64_RS09405 at 10.331 kb on + strand, within COO64_RS09405 at 10.331 kb on + strand, within COO64_RS09405 at 10.332 kb on - strand, within COO64_RS09405 at 10.482 kb on - strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.515 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.517 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.519 kb on + strand at 10.521 kb on + strand at 10.521 kb on + strand at 10.521 kb on + strand at 10.539 kb on + strand at 12.899 kb on + strand, within topA at 12.899 kb on + strand, within topA at 12.900 kb on - strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.903 kb on + strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 12.904 kb on - strand, within topA at 13.026 kb on + strand at 13.027 kb on - strand at 13.027 kb on - strand at 13.027 kb on - strand at 13.027 kb on - strand at 13.027 kb on - strand at 13.110 kb on + strand at 13.111 kb on - strand at 13.295 kb on + strand at 13.295 kb on + strand at 13.352 kb on + strand at 13.352 kb on + strand at 13.352 kb on + strand at 13.352 kb on + strand at 13.353 kb on - strand at 13.353 kb on - strand at 13.376 kb on + strand at 13.526 kb on + strand, within COO64_RS09415 at 13.526 kb on + strand, within COO64_RS09415 at 13.662 kb on + strand, within COO64_RS09415 at 13.663 kb on - strand, within COO64_RS09415 at 13.663 kb on - strand, within COO64_RS09415 at 13.663 kb on - strand, within COO64_RS09415 at 13.663 kb on - strand, within COO64_RS09415 at 13.670 kb on + strand, within COO64_RS09415 at 13.670 kb on + strand, within COO64_RS09415 at 13.746 kb on + strand, within COO64_RS09415 at 13.747 kb on - strand, within COO64_RS09415 at 13.823 kb on - strand at 13.875 kb on + strand at 13.875 kb on + strand at 13.875 kb on + strand at 13.909 kb on - strand at 14.097 kb on - strand, within COO64_RS09420
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 9,681 + fadA COO64_RS09400 0.60 -0.2 10,239 + +0.3 10,240 - -0.5 10,240 - +1.2 10,240 - -0.7 10,240 - +0.3 10,240 - +0.2 10,251 + +0.5 10,252 - -0.7 10,252 - +0.1 10,252 - +0.9 10,252 - +3.2 10,254 - +0.0 10,262 + COO64_RS09405 0.12 -0.4 10,262 + COO64_RS09405 0.12 -0.5 10,262 + COO64_RS09405 0.12 -0.6 10,263 - COO64_RS09405 0.12 -0.1 10,263 - COO64_RS09405 0.12 +0.0 10,263 - COO64_RS09405 0.12 +0.2 10,277 + COO64_RS09405 0.18 -0.7 10,278 - COO64_RS09405 0.18 -0.2 10,331 + COO64_RS09405 0.39 -0.5 10,331 + COO64_RS09405 0.39 +0.2 10,331 + COO64_RS09405 0.39 +0.1 10,332 - COO64_RS09405 0.40 +0.6 10,482 - -0.6 10,515 + +0.7 10,515 + +0.8 10,515 + +0.2 10,515 + -0.5 10,515 + +0.4 10,515 + -2.1 10,515 + -0.8 10,515 + -1.3 10,515 + +0.7 10,515 + +0.0 10,515 + +1.1 10,517 + -0.9 10,517 + +0.0 10,517 + -0.4 10,517 + -0.2 10,517 + -0.4 10,517 + +0.8 10,517 + -0.7 10,517 + +0.5 10,517 + -0.9 10,517 + +0.4 10,517 + -0.4 10,517 + +0.8 10,517 + +0.9 10,517 + -1.7 10,517 + +0.3 10,517 + +0.7 10,517 + +0.2 10,517 + +0.7 10,517 + -1.8 10,517 + -1.4 10,519 + +1.0 10,519 + -0.1 10,519 + +0.7 10,519 + -0.4 10,519 + +1.1 10,519 + -0.4 10,519 + -0.9 10,519 + -1.1 10,519 + +0.4 10,519 + -0.2 10,519 + -0.7 10,519 + +1.3 10,519 + -0.4 10,519 + +0.2 10,519 + -0.4 10,519 + -0.8 10,519 + -1.5 10,519 + +0.0 10,519 + +0.7 10,519 + -0.1 10,519 + +0.2 10,521 + +0.2 10,521 + +0.1 10,521 + +0.9 10,539 + +0.5 12,899 + topA COO64_RS09410 0.89 -1.1 12,899 + topA COO64_RS09410 0.89 -0.2 12,900 - topA COO64_RS09410 0.89 -1.1 12,903 + topA COO64_RS09410 0.89 -0.1 12,903 + topA COO64_RS09410 0.89 -1.1 12,903 + topA COO64_RS09410 0.89 +0.7 12,903 + topA COO64_RS09410 0.89 -0.1 12,903 + topA COO64_RS09410 0.89 +0.5 12,903 + topA COO64_RS09410 0.89 -1.2 12,903 + topA COO64_RS09410 0.89 -0.5 12,903 + topA COO64_RS09410 0.89 -0.7 12,903 + topA COO64_RS09410 0.89 -0.4 12,903 + topA COO64_RS09410 0.89 -0.1 12,903 + topA COO64_RS09410 0.89 +0.3 12,903 + topA COO64_RS09410 0.89 -0.1 12,903 + topA COO64_RS09410 0.89 +0.9 12,903 + topA COO64_RS09410 0.89 -0.7 12,904 - topA COO64_RS09410 0.89 -1.0 12,904 - topA COO64_RS09410 0.89 -0.2 12,904 - topA COO64_RS09410 0.89 -0.1 12,904 - topA COO64_RS09410 0.89 +0.0 12,904 - topA COO64_RS09410 0.89 +1.9 12,904 - topA COO64_RS09410 0.89 +0.3 12,904 - topA COO64_RS09410 0.89 -1.4 12,904 - topA COO64_RS09410 0.89 -1.1 12,904 - topA COO64_RS09410 0.89 -0.9 12,904 - topA COO64_RS09410 0.89 -0.9 12,904 - topA COO64_RS09410 0.89 -1.1 12,904 - topA COO64_RS09410 0.89 +0.0 12,904 - topA COO64_RS09410 0.89 +0.6 12,904 - topA COO64_RS09410 0.89 -0.4 12,904 - topA COO64_RS09410 0.89 -0.1 13,026 + +0.2 13,027 - -1.2 13,027 - +0.9 13,027 - -0.1 13,027 - -0.1 13,027 - -0.9 13,110 + +0.1 13,111 - -0.7 13,295 + +0.0 13,295 + +0.5 13,352 + -0.1 13,352 + +0.0 13,352 + +0.2 13,352 + -0.1 13,353 - -0.8 13,353 - -1.0 13,376 + -1.0 13,526 + COO64_RS09415 0.36 +0.3 13,526 + COO64_RS09415 0.36 -0.1 13,662 + COO64_RS09415 0.62 -0.0 13,663 - COO64_RS09415 0.62 +0.9 13,663 - COO64_RS09415 0.62 -0.2 13,663 - COO64_RS09415 0.62 +0.9 13,663 - COO64_RS09415 0.62 +0.7 13,670 + COO64_RS09415 0.64 -0.4 13,670 + COO64_RS09415 0.64 +0.1 13,746 + COO64_RS09415 0.78 +0.4 13,747 - COO64_RS09415 0.79 -0.2 13,823 - -1.3 13,875 + -0.3 13,875 + +0.3 13,875 + -2.4 13,909 - +0.1 14,097 - COO64_RS09420 0.80 +1.3
Or see this region's nucleotide sequence