Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS07735 and COO64_RS07740 overlap by 20 nucleotides COO64_RS07740 and COO64_RS07745 are separated by 79 nucleotides COO64_RS07745 and COO64_RS07750 are separated by 81 nucleotides
COO64_RS07735: COO64_RS07735 - bestrophin family protein, at 38,448 to 39,365
_RS07735
COO64_RS07740: COO64_RS07740 - bile acid:sodium symporter family protein, at 39,346 to 40,338
_RS07740
COO64_RS07745: COO64_RS07745 - helix-turn-helix domain-containing protein, at 40,418 to 41,233
_RS07745
COO64_RS07750: COO64_RS07750 - hypothetical protein, at 41,315 to 41,710
_RS07750
Position (kb)
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1 at 38.353 kb on + strand at 38.353 kb on + strand at 38.353 kb on + strand at 38.353 kb on + strand at 38.353 kb on + strand at 38.353 kb on + strand at 38.353 kb on + strand at 38.354 kb on - strand at 38.354 kb on - strand at 38.354 kb on - strand at 38.354 kb on - strand at 38.378 kb on + strand at 38.378 kb on + strand at 38.378 kb on + strand at 38.402 kb on + strand at 38.402 kb on + strand at 38.402 kb on + strand at 38.538 kb on + strand at 38.538 kb on + strand at 38.539 kb on - strand at 38.539 kb on - strand at 38.545 kb on + strand, within COO64_RS07735 at 38.546 kb on - strand, within COO64_RS07735 at 38.546 kb on - strand, within COO64_RS07735 at 38.592 kb on + strand, within COO64_RS07735 at 38.599 kb on + strand, within COO64_RS07735 at 38.599 kb on + strand, within COO64_RS07735 at 38.600 kb on - strand, within COO64_RS07735 at 38.600 kb on - strand, within COO64_RS07735 at 38.677 kb on - strand, within COO64_RS07735 at 38.820 kb on + strand, within COO64_RS07735 at 38.820 kb on + strand, within COO64_RS07735 at 39.033 kb on + strand, within COO64_RS07735 at 39.033 kb on + strand, within COO64_RS07735 at 39.033 kb on + strand, within COO64_RS07735 at 39.034 kb on - strand, within COO64_RS07735 at 39.084 kb on + strand, within COO64_RS07735 at 39.084 kb on + strand, within COO64_RS07735 at 39.084 kb on + strand, within COO64_RS07735 at 39.085 kb on - strand, within COO64_RS07735 at 39.085 kb on - strand, within COO64_RS07735 at 39.085 kb on - strand, within COO64_RS07735 at 39.085 kb on - strand, within COO64_RS07735 at 39.118 kb on - strand, within COO64_RS07735 at 39.192 kb on + strand, within COO64_RS07735 at 39.192 kb on + strand, within COO64_RS07735 at 39.363 kb on + strand at 39.393 kb on - strand at 39.707 kb on + strand, within COO64_RS07740 at 39.982 kb on - strand, within COO64_RS07740 at 40.025 kb on + strand, within COO64_RS07740 at 40.025 kb on + strand, within COO64_RS07740 at 40.026 kb on - strand, within COO64_RS07740 at 40.026 kb on - strand, within COO64_RS07740 at 40.040 kb on + strand, within COO64_RS07740 at 40.041 kb on - strand, within COO64_RS07740 at 40.123 kb on + strand, within COO64_RS07740 at 40.316 kb on - strand at 40.332 kb on - strand at 40.356 kb on + strand at 40.493 kb on + strand at 40.493 kb on + strand at 40.494 kb on - strand at 40.494 kb on - strand at 40.494 kb on - strand at 40.565 kb on + strand, within COO64_RS07745 at 40.566 kb on - strand, within COO64_RS07745 at 40.566 kb on - strand, within COO64_RS07745 at 40.700 kb on + strand, within COO64_RS07745 at 40.700 kb on + strand, within COO64_RS07745 at 40.809 kb on - strand, within COO64_RS07745 at 41.156 kb on - strand at 41.156 kb on - strand at 41.231 kb on + strand at 41.231 kb on + strand at 41.231 kb on + strand at 41.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 38,353 + +0.2 38,353 + +1.1 38,353 + -0.3 38,353 + -0.0 38,353 + +0.3 38,353 + +1.2 38,353 + -0.3 38,354 - +0.2 38,354 - -0.2 38,354 - +0.2 38,354 - -1.0 38,378 + +0.9 38,378 + -0.6 38,378 + -0.9 38,402 + -0.9 38,402 + -0.0 38,402 + -0.5 38,538 + -0.4 38,538 + +0.5 38,539 - -0.6 38,539 - +0.5 38,545 + COO64_RS07735 0.11 +0.8 38,546 - COO64_RS07735 0.11 +0.1 38,546 - COO64_RS07735 0.11 +0.1 38,592 + COO64_RS07735 0.16 +0.3 38,599 + COO64_RS07735 0.16 -1.1 38,599 + COO64_RS07735 0.16 +0.0 38,600 - COO64_RS07735 0.17 -0.8 38,600 - COO64_RS07735 0.17 -0.6 38,677 - COO64_RS07735 0.25 +0.5 38,820 + COO64_RS07735 0.41 +1.4 38,820 + COO64_RS07735 0.41 -0.7 39,033 + COO64_RS07735 0.64 +0.0 39,033 + COO64_RS07735 0.64 +1.1 39,033 + COO64_RS07735 0.64 -1.8 39,034 - COO64_RS07735 0.64 -0.1 39,084 + COO64_RS07735 0.69 -0.3 39,084 + COO64_RS07735 0.69 -1.3 39,084 + COO64_RS07735 0.69 -0.0 39,085 - COO64_RS07735 0.69 -0.0 39,085 - COO64_RS07735 0.69 +0.1 39,085 - COO64_RS07735 0.69 +0.3 39,085 - COO64_RS07735 0.69 -2.2 39,118 - COO64_RS07735 0.73 -0.4 39,192 + COO64_RS07735 0.81 +0.3 39,192 + COO64_RS07735 0.81 -1.0 39,363 + -0.2 39,393 - +0.3 39,707 + COO64_RS07740 0.36 -0.5 39,982 - COO64_RS07740 0.64 -0.8 40,025 + COO64_RS07740 0.68 +0.5 40,025 + COO64_RS07740 0.68 -0.9 40,026 - COO64_RS07740 0.68 -0.6 40,026 - COO64_RS07740 0.68 +0.6 40,040 + COO64_RS07740 0.70 -0.8 40,041 - COO64_RS07740 0.70 +0.3 40,123 + COO64_RS07740 0.78 +0.2 40,316 - -0.0 40,332 - -0.5 40,356 + +0.3 40,493 + -0.7 40,493 + -0.9 40,494 - +0.4 40,494 - +0.1 40,494 - +0.3 40,565 + COO64_RS07745 0.18 +0.0 40,566 - COO64_RS07745 0.18 -0.6 40,566 - COO64_RS07745 0.18 -0.8 40,700 + COO64_RS07745 0.35 +0.7 40,700 + COO64_RS07745 0.35 -0.7 40,809 - COO64_RS07745 0.48 +0.1 41,156 - +0.8 41,156 - -0.6 41,231 + -1.0 41,231 + -0.2 41,231 + +0.8 41,269 - -0.1
Or see this region's nucleotide sequence