Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purN and COO64_RS07305 are separated by 8 nucleotides COO64_RS07305 and COO64_RS07310 are separated by 194 nucleotides COO64_RS07310 and COO64_RS07315 are separated by 45 nucleotides COO64_RS07315 and mazG are separated by 23 nucleotides
COO64_RS07300: purN - phosphoribosylglycinamide formyltransferase, at 974,235 to 974,888
purN
COO64_RS07305: COO64_RS07305 - DUF3108 domain-containing protein, at 974,897 to 975,607
_RS07305
COO64_RS07310: COO64_RS07310 - hypothetical protein, at 975,802 to 975,984
_RS07310
COO64_RS07315: COO64_RS07315 - DUF2058 domain-containing protein, at 976,030 to 976,569
_RS07315
COO64_RS07320: mazG - nucleoside triphosphate pyrophosphohydrolase, at 976,593 to 977,423
mazG
Position (kb) 975
976 Strain fitness (log2 ratio) -2
-1
0
1
2 at 974.840 kb on - strand at 974.840 kb on - strand at 974.896 kb on + strand at 974.978 kb on + strand, within COO64_RS07305 at 974.978 kb on + strand, within COO64_RS07305 at 974.979 kb on - strand, within COO64_RS07305 at 975.107 kb on - strand, within COO64_RS07305 at 975.107 kb on - strand, within COO64_RS07305 at 975.146 kb on + strand, within COO64_RS07305 at 975.146 kb on + strand, within COO64_RS07305 at 975.146 kb on + strand, within COO64_RS07305 at 975.146 kb on + strand, within COO64_RS07305 at 975.147 kb on - strand, within COO64_RS07305 at 975.147 kb on - strand, within COO64_RS07305 at 975.287 kb on + strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.288 kb on - strand, within COO64_RS07305 at 975.338 kb on + strand, within COO64_RS07305 at 975.338 kb on + strand, within COO64_RS07305 at 975.338 kb on + strand, within COO64_RS07305 at 975.338 kb on + strand, within COO64_RS07305 at 975.338 kb on + strand, within COO64_RS07305 at 975.339 kb on - strand, within COO64_RS07305 at 975.339 kb on - strand, within COO64_RS07305 at 975.367 kb on + strand, within COO64_RS07305 at 975.368 kb on - strand, within COO64_RS07305 at 975.368 kb on - strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.371 kb on + strand, within COO64_RS07305 at 975.372 kb on - strand, within COO64_RS07305 at 975.372 kb on - strand, within COO64_RS07305 at 975.506 kb on + strand, within COO64_RS07305 at 975.506 kb on + strand, within COO64_RS07305 at 975.506 kb on + strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.507 kb on - strand, within COO64_RS07305 at 975.516 kb on + strand, within COO64_RS07305 at 975.516 kb on + strand, within COO64_RS07305 at 975.517 kb on - strand, within COO64_RS07305 at 975.551 kb on + strand at 975.551 kb on + strand at 975.551 kb on + strand at 975.551 kb on + strand at 975.551 kb on + strand at 975.551 kb on + strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.552 kb on - strand at 975.574 kb on + strand at 975.608 kb on + strand at 975.624 kb on + strand at 975.658 kb on + strand at 975.695 kb on + strand at 975.695 kb on + strand at 975.696 kb on - strand at 975.727 kb on - strand at 975.730 kb on + strand at 975.730 kb on + strand at 975.731 kb on - strand at 975.731 kb on - strand at 975.738 kb on + strand at 975.749 kb on - strand at 975.815 kb on + strand at 975.816 kb on - strand at 975.816 kb on - strand at 975.955 kb on + strand, within COO64_RS07310 at 975.956 kb on - strand, within COO64_RS07310 at 975.956 kb on - strand, within COO64_RS07310 at 975.956 kb on - strand, within COO64_RS07310 at 975.956 kb on - strand, within COO64_RS07310 at 975.982 kb on + strand at 975.982 kb on + strand at 976.029 kb on - strand at 976.031 kb on + strand at 976.031 kb on + strand at 976.031 kb on + strand at 976.031 kb on + strand at 976.032 kb on - strand at 976.032 kb on - strand at 976.032 kb on - strand at 976.059 kb on - strand at 976.059 kb on - strand at 976.116 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.277 kb on + strand, within COO64_RS07315 at 976.278 kb on - strand, within COO64_RS07315 at 976.278 kb on - strand, within COO64_RS07315 at 976.278 kb on - strand, within COO64_RS07315 at 976.280 kb on + strand, within COO64_RS07315 at 976.280 kb on + strand, within COO64_RS07315 at 976.280 kb on + strand, within COO64_RS07315 at 976.280 kb on + strand, within COO64_RS07315 at 976.280 kb on + strand, within COO64_RS07315 at 976.281 kb on - strand, within COO64_RS07315 at 976.281 kb on - strand, within COO64_RS07315 at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.582 kb on + strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.583 kb on - strand at 976.686 kb on + strand, within mazG at 976.686 kb on + strand, within mazG at 976.686 kb on + strand, within mazG at 976.686 kb on + strand, within mazG at 976.687 kb on - strand, within mazG at 976.687 kb on - strand, within mazG at 976.772 kb on + strand, within mazG at 976.773 kb on - strand, within mazG at 976.773 kb on - strand, within mazG
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 974,840 - +0.4 974,840 - -0.9 974,896 + +0.1 974,978 + COO64_RS07305 0.11 +0.2 974,978 + COO64_RS07305 0.11 +0.1 974,979 - COO64_RS07305 0.12 -0.9 975,107 - COO64_RS07305 0.30 +0.1 975,107 - COO64_RS07305 0.30 -0.2 975,146 + COO64_RS07305 0.35 -0.5 975,146 + COO64_RS07305 0.35 -1.5 975,146 + COO64_RS07305 0.35 +0.5 975,146 + COO64_RS07305 0.35 -0.4 975,147 - COO64_RS07305 0.35 +0.1 975,147 - COO64_RS07305 0.35 -1.7 975,287 + COO64_RS07305 0.55 +0.1 975,288 - COO64_RS07305 0.55 +0.7 975,288 - COO64_RS07305 0.55 +0.1 975,288 - COO64_RS07305 0.55 -0.6 975,288 - COO64_RS07305 0.55 -0.5 975,288 - COO64_RS07305 0.55 -0.8 975,288 - COO64_RS07305 0.55 -0.9 975,288 - COO64_RS07305 0.55 +0.7 975,338 + COO64_RS07305 0.62 -0.7 975,338 + COO64_RS07305 0.62 +0.5 975,338 + COO64_RS07305 0.62 -1.0 975,338 + COO64_RS07305 0.62 -0.4 975,338 + COO64_RS07305 0.62 -1.4 975,339 - COO64_RS07305 0.62 -1.1 975,339 - COO64_RS07305 0.62 -0.5 975,367 + COO64_RS07305 0.66 +0.6 975,368 - COO64_RS07305 0.66 -1.2 975,368 - COO64_RS07305 0.66 -0.4 975,371 + COO64_RS07305 0.67 -1.1 975,371 + COO64_RS07305 0.67 +0.1 975,371 + COO64_RS07305 0.67 -0.1 975,371 + COO64_RS07305 0.67 +0.4 975,371 + COO64_RS07305 0.67 -0.2 975,371 + COO64_RS07305 0.67 -0.0 975,371 + COO64_RS07305 0.67 -1.1 975,372 - COO64_RS07305 0.67 +0.2 975,372 - COO64_RS07305 0.67 -0.0 975,506 + COO64_RS07305 0.86 -2.0 975,506 + COO64_RS07305 0.86 -0.8 975,506 + COO64_RS07305 0.86 -0.7 975,507 - COO64_RS07305 0.86 -0.1 975,507 - COO64_RS07305 0.86 +0.1 975,507 - COO64_RS07305 0.86 +1.0 975,507 - COO64_RS07305 0.86 -0.2 975,507 - COO64_RS07305 0.86 +0.5 975,507 - COO64_RS07305 0.86 +0.1 975,516 + COO64_RS07305 0.87 -0.7 975,516 + COO64_RS07305 0.87 -0.7 975,517 - COO64_RS07305 0.87 -0.5 975,551 + -0.4 975,551 + -0.1 975,551 + -0.6 975,551 + -1.1 975,551 + -2.4 975,551 + +0.5 975,552 - +1.5 975,552 - -1.6 975,552 - +0.4 975,552 - -0.2 975,552 - -0.6 975,552 - -0.8 975,552 - +0.1 975,552 - -1.1 975,552 - +0.1 975,552 - +2.2 975,552 - +0.0 975,574 + +0.0 975,608 + +0.7 975,624 + +0.4 975,658 + -0.2 975,695 + -1.2 975,695 + +0.1 975,696 - +0.4 975,727 - +0.0 975,730 + -0.1 975,730 + +0.0 975,731 - -1.0 975,731 - +1.5 975,738 + +0.0 975,749 - -0.6 975,815 + -0.4 975,816 - -0.1 975,816 - -1.0 975,955 + COO64_RS07310 0.84 -1.5 975,956 - COO64_RS07310 0.84 -0.3 975,956 - COO64_RS07310 0.84 -0.1 975,956 - COO64_RS07310 0.84 +0.6 975,956 - COO64_RS07310 0.84 -0.4 975,982 + -0.1 975,982 + +0.0 976,029 - -0.7 976,031 + +0.5 976,031 + +0.3 976,031 + -1.1 976,031 + +0.7 976,032 - -1.1 976,032 - -0.3 976,032 - +0.1 976,059 - -0.3 976,059 - +0.4 976,116 + COO64_RS07315 0.16 -0.1 976,277 + COO64_RS07315 0.46 -0.8 976,277 + COO64_RS07315 0.46 +0.3 976,277 + COO64_RS07315 0.46 -0.6 976,277 + COO64_RS07315 0.46 +0.9 976,277 + COO64_RS07315 0.46 -0.3 976,277 + COO64_RS07315 0.46 -0.5 976,277 + COO64_RS07315 0.46 -0.2 976,277 + COO64_RS07315 0.46 -0.2 976,278 - COO64_RS07315 0.46 +1.2 976,278 - COO64_RS07315 0.46 +0.3 976,278 - COO64_RS07315 0.46 +0.4 976,280 + COO64_RS07315 0.46 -1.0 976,280 + COO64_RS07315 0.46 +0.3 976,280 + COO64_RS07315 0.46 -0.3 976,280 + COO64_RS07315 0.46 +0.6 976,280 + COO64_RS07315 0.46 +0.4 976,281 - COO64_RS07315 0.46 +1.5 976,281 - COO64_RS07315 0.46 +1.1 976,582 + -0.3 976,582 + +0.1 976,582 + -0.2 976,582 + -2.4 976,582 + -1.2 976,582 + -0.9 976,582 + +0.1 976,582 + -0.8 976,582 + +1.6 976,582 + +0.8 976,582 + +0.7 976,582 + +0.3 976,583 - -2.5 976,583 - +0.6 976,583 - +1.0 976,583 - +0.7 976,583 - -0.9 976,583 - +0.9 976,583 - -0.1 976,583 - +0.5 976,583 - -0.6 976,583 - -0.2 976,583 - +0.0 976,583 - -0.7 976,686 + mazG COO64_RS07320 0.11 -0.1 976,686 + mazG COO64_RS07320 0.11 +0.2 976,686 + mazG COO64_RS07320 0.11 -0.7 976,686 + mazG COO64_RS07320 0.11 -0.3 976,687 - mazG COO64_RS07320 0.11 +0.7 976,687 - mazG COO64_RS07320 0.11 -1.0 976,772 + mazG COO64_RS07320 0.22 -1.0 976,773 - mazG COO64_RS07320 0.22 +0.4 976,773 - mazG COO64_RS07320 0.22 +0.2
Or see this region's nucleotide sequence