Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS06570 and pyrF overlap by 11 nucleotides pyrF and COO64_RS06580 are separated by 179 nucleotides COO64_RS06580 and COO64_RS06585 are separated by 30 nucleotides
COO64_RS06570: COO64_RS06570 - NADP-dependent oxidoreductase, at 809,752 to 810,756
_RS06570
COO64_RS06575: pyrF - orotidine-5'-phosphate decarboxylase, at 810,746 to 811,447
pyrF
COO64_RS06580: COO64_RS06580 - DUF2897 family protein, at 811,627 to 811,794
_RS06580
COO64_RS06585: COO64_RS06585 - SDR family oxidoreductase, at 811,825 to 812,619
_RS06585
Position (kb) 810
811
812 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 809.827 kb on + strand at 809.827 kb on + strand at 809.828 kb on - strand at 809.828 kb on - strand at 809.828 kb on - strand at 809.828 kb on - strand at 809.828 kb on - strand at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.884 kb on + strand, within COO64_RS06570 at 809.885 kb on - strand, within COO64_RS06570 at 809.885 kb on - strand, within COO64_RS06570 at 809.885 kb on - strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.935 kb on + strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.936 kb on - strand, within COO64_RS06570 at 809.955 kb on - strand, within COO64_RS06570 at 809.955 kb on - strand, within COO64_RS06570 at 810.058 kb on + strand, within COO64_RS06570 at 810.058 kb on + strand, within COO64_RS06570 at 810.059 kb on - strand, within COO64_RS06570 at 810.059 kb on - strand, within COO64_RS06570 at 810.059 kb on - strand, within COO64_RS06570 at 810.059 kb on - strand, within COO64_RS06570 at 810.085 kb on + strand, within COO64_RS06570 at 810.085 kb on + strand, within COO64_RS06570 at 810.085 kb on + strand, within COO64_RS06570 at 810.085 kb on + strand, within COO64_RS06570 at 810.086 kb on - strand, within COO64_RS06570 at 810.121 kb on + strand, within COO64_RS06570 at 810.122 kb on - strand, within COO64_RS06570 at 810.157 kb on + strand, within COO64_RS06570 at 810.158 kb on - strand, within COO64_RS06570 at 810.310 kb on + strand, within COO64_RS06570 at 810.349 kb on + strand, within COO64_RS06570 at 810.349 kb on + strand, within COO64_RS06570 at 810.349 kb on + strand, within COO64_RS06570 at 810.349 kb on + strand, within COO64_RS06570 at 810.350 kb on - strand, within COO64_RS06570 at 810.350 kb on - strand, within COO64_RS06570 at 810.350 kb on - strand, within COO64_RS06570 at 810.350 kb on - strand, within COO64_RS06570 at 810.350 kb on - strand, within COO64_RS06570 at 810.409 kb on + strand, within COO64_RS06570 at 810.410 kb on - strand, within COO64_RS06570 at 810.410 kb on - strand, within COO64_RS06570 at 810.410 kb on - strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.502 kb on + strand, within COO64_RS06570 at 810.503 kb on - strand, within COO64_RS06570 at 810.503 kb on - strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.538 kb on + strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.539 kb on - strand, within COO64_RS06570 at 810.604 kb on + strand, within COO64_RS06570 at 810.604 kb on + strand, within COO64_RS06570 at 810.605 kb on - strand, within COO64_RS06570 at 810.605 kb on - strand, within COO64_RS06570 at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.747 kb on + strand at 810.748 kb on - strand at 810.748 kb on - strand at 810.748 kb on - strand at 810.748 kb on - strand at 810.829 kb on - strand, within pyrF at 811.246 kb on + strand, within pyrF at 811.247 kb on - strand, within pyrF at 811.486 kb on + strand at 811.487 kb on - strand at 811.487 kb on - strand at 811.551 kb on - strand at 811.551 kb on - strand at 811.551 kb on - strand at 811.551 kb on - strand at 811.551 kb on - strand at 811.552 kb on + strand at 811.552 kb on + strand at 811.552 kb on + strand at 811.552 kb on + strand at 811.552 kb on + strand at 811.553 kb on - strand at 811.553 kb on - strand at 811.588 kb on - strand at 811.612 kb on + strand at 811.612 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.624 kb on + strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.625 kb on - strand at 811.636 kb on + strand at 811.637 kb on - strand at 811.637 kb on - strand at 811.737 kb on + strand, within COO64_RS06580 at 811.738 kb on - strand, within COO64_RS06580 at 811.738 kb on - strand, within COO64_RS06580 at 811.755 kb on + strand, within COO64_RS06580 at 811.755 kb on + strand, within COO64_RS06580 at 811.755 kb on + strand, within COO64_RS06580 at 811.756 kb on - strand, within COO64_RS06580 at 811.756 kb on - strand, within COO64_RS06580 at 811.827 kb on - strand at 811.832 kb on + strand at 811.833 kb on - strand at 811.833 kb on - strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.892 kb on + strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.893 kb on - strand at 811.984 kb on + strand, within COO64_RS06585 at 811.985 kb on - strand, within COO64_RS06585 at 812.018 kb on - strand, within COO64_RS06585 at 812.018 kb on - strand, within COO64_RS06585 at 812.018 kb on - strand, within COO64_RS06585 at 812.018 kb on - strand, within COO64_RS06585 at 812.018 kb on - strand, within COO64_RS06585 at 812.050 kb on - strand, within COO64_RS06585 at 812.065 kb on + strand, within COO64_RS06585 at 812.083 kb on + strand, within COO64_RS06585 at 812.091 kb on + strand, within COO64_RS06585 at 812.091 kb on + strand, within COO64_RS06585 at 812.135 kb on + strand, within COO64_RS06585 at 812.135 kb on + strand, within COO64_RS06585 at 812.135 kb on + strand, within COO64_RS06585 at 812.135 kb on + strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.136 kb on - strand, within COO64_RS06585 at 812.186 kb on - strand, within COO64_RS06585 at 812.186 kb on - strand, within COO64_RS06585 at 812.209 kb on + strand, within COO64_RS06585 at 812.209 kb on + strand, within COO64_RS06585 at 812.209 kb on + strand, within COO64_RS06585 at 812.210 kb on - strand, within COO64_RS06585 at 812.210 kb on - strand, within COO64_RS06585 at 812.210 kb on - strand, within COO64_RS06585 at 812.210 kb on - strand, within COO64_RS06585 at 812.274 kb on + strand, within COO64_RS06585 at 812.354 kb on - strand, within COO64_RS06585 at 812.367 kb on + strand, within COO64_RS06585
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 809,827 + +0.7 809,827 + -0.1 809,828 - -1.1 809,828 - -0.3 809,828 - -1.3 809,828 - +0.3 809,828 - -0.1 809,884 + COO64_RS06570 0.13 -0.8 809,884 + COO64_RS06570 0.13 +0.1 809,884 + COO64_RS06570 0.13 -0.1 809,884 + COO64_RS06570 0.13 -0.5 809,884 + COO64_RS06570 0.13 +0.7 809,884 + COO64_RS06570 0.13 +1.2 809,884 + COO64_RS06570 0.13 +1.3 809,884 + COO64_RS06570 0.13 +0.7 809,885 - COO64_RS06570 0.13 +0.6 809,885 - COO64_RS06570 0.13 -0.7 809,885 - COO64_RS06570 0.13 -1.0 809,935 + COO64_RS06570 0.18 +0.1 809,935 + COO64_RS06570 0.18 +0.3 809,935 + COO64_RS06570 0.18 -1.9 809,935 + COO64_RS06570 0.18 +0.8 809,935 + COO64_RS06570 0.18 -0.0 809,935 + COO64_RS06570 0.18 -0.3 809,935 + COO64_RS06570 0.18 -0.1 809,935 + COO64_RS06570 0.18 -1.9 809,935 + COO64_RS06570 0.18 +0.0 809,935 + COO64_RS06570 0.18 +0.1 809,935 + COO64_RS06570 0.18 +0.1 809,935 + COO64_RS06570 0.18 -2.4 809,935 + COO64_RS06570 0.18 -0.6 809,935 + COO64_RS06570 0.18 +0.3 809,936 - COO64_RS06570 0.18 +0.1 809,936 - COO64_RS06570 0.18 -0.4 809,936 - COO64_RS06570 0.18 -1.0 809,936 - COO64_RS06570 0.18 +0.0 809,936 - COO64_RS06570 0.18 -0.9 809,936 - COO64_RS06570 0.18 -1.2 809,936 - COO64_RS06570 0.18 -0.1 809,936 - COO64_RS06570 0.18 +0.1 809,955 - COO64_RS06570 0.20 +1.5 809,955 - COO64_RS06570 0.20 +0.3 810,058 + COO64_RS06570 0.30 -0.1 810,058 + COO64_RS06570 0.30 -0.6 810,059 - COO64_RS06570 0.31 +0.2 810,059 - COO64_RS06570 0.31 +1.1 810,059 - COO64_RS06570 0.31 -1.1 810,059 - COO64_RS06570 0.31 -0.7 810,085 + COO64_RS06570 0.33 +0.3 810,085 + COO64_RS06570 0.33 -0.1 810,085 + COO64_RS06570 0.33 -0.0 810,085 + COO64_RS06570 0.33 +0.3 810,086 - COO64_RS06570 0.33 -0.4 810,121 + COO64_RS06570 0.37 -0.3 810,122 - COO64_RS06570 0.37 +0.3 810,157 + COO64_RS06570 0.40 +0.3 810,158 - COO64_RS06570 0.40 +0.6 810,310 + COO64_RS06570 0.56 -0.7 810,349 + COO64_RS06570 0.59 -1.0 810,349 + COO64_RS06570 0.59 -0.3 810,349 + COO64_RS06570 0.59 +0.4 810,349 + COO64_RS06570 0.59 -0.3 810,350 - COO64_RS06570 0.60 -1.3 810,350 - COO64_RS06570 0.60 -0.5 810,350 - COO64_RS06570 0.60 -0.3 810,350 - COO64_RS06570 0.60 -1.1 810,350 - COO64_RS06570 0.60 -0.3 810,409 + COO64_RS06570 0.65 -0.4 810,410 - COO64_RS06570 0.65 +1.4 810,410 - COO64_RS06570 0.65 -0.3 810,410 - COO64_RS06570 0.65 -0.4 810,502 + COO64_RS06570 0.75 +0.3 810,502 + COO64_RS06570 0.75 +0.1 810,502 + COO64_RS06570 0.75 -0.7 810,502 + COO64_RS06570 0.75 -0.1 810,502 + COO64_RS06570 0.75 +0.9 810,502 + COO64_RS06570 0.75 -0.0 810,502 + COO64_RS06570 0.75 -0.9 810,502 + COO64_RS06570 0.75 -0.6 810,503 - COO64_RS06570 0.75 -0.7 810,503 - COO64_RS06570 0.75 +0.1 810,538 + COO64_RS06570 0.78 -0.7 810,538 + COO64_RS06570 0.78 -0.2 810,538 + COO64_RS06570 0.78 +0.6 810,538 + COO64_RS06570 0.78 +0.9 810,538 + COO64_RS06570 0.78 +0.8 810,538 + COO64_RS06570 0.78 -0.8 810,538 + COO64_RS06570 0.78 +0.2 810,538 + COO64_RS06570 0.78 -0.3 810,539 - COO64_RS06570 0.78 -0.0 810,539 - COO64_RS06570 0.78 -1.1 810,539 - COO64_RS06570 0.78 -0.1 810,539 - COO64_RS06570 0.78 +0.8 810,539 - COO64_RS06570 0.78 +0.1 810,539 - COO64_RS06570 0.78 -1.9 810,539 - COO64_RS06570 0.78 -2.4 810,539 - COO64_RS06570 0.78 +0.5 810,539 - COO64_RS06570 0.78 -2.4 810,539 - COO64_RS06570 0.78 -0.2 810,604 + COO64_RS06570 0.85 +1.3 810,604 + COO64_RS06570 0.85 +0.8 810,605 - COO64_RS06570 0.85 -0.9 810,605 - COO64_RS06570 0.85 -0.3 810,747 + +0.7 810,747 + +0.4 810,747 + +0.2 810,747 + +1.0 810,747 + +0.3 810,747 + +0.5 810,747 + -0.3 810,747 + -0.4 810,748 - +0.2 810,748 - -0.4 810,748 - +0.3 810,748 - +1.9 810,829 - pyrF COO64_RS06575 0.12 -2.8 811,246 + pyrF COO64_RS06575 0.71 +0.2 811,247 - pyrF COO64_RS06575 0.71 -0.1 811,486 + -0.6 811,487 - -1.0 811,487 - -0.4 811,551 - +0.1 811,551 - -2.1 811,551 - +0.2 811,551 - -0.8 811,551 - -0.0 811,552 + +0.1 811,552 + -0.9 811,552 + -0.4 811,552 + -0.3 811,552 + -1.1 811,553 - +0.5 811,553 - +1.5 811,588 - +0.5 811,612 + -0.1 811,612 + +0.9 811,624 + -0.7 811,624 + -0.4 811,624 + -0.5 811,624 + -1.2 811,624 + +0.3 811,624 + -0.7 811,624 + -0.0 811,624 + +0.5 811,624 + +0.9 811,624 + +0.2 811,624 + -0.5 811,624 + -0.3 811,624 + -0.9 811,624 + +0.8 811,625 - -0.0 811,625 - +0.7 811,625 - -0.2 811,625 - -0.9 811,625 - +0.9 811,625 - -0.1 811,625 - -0.8 811,625 - +0.5 811,636 + +0.7 811,637 - +0.9 811,637 - -0.7 811,737 + COO64_RS06580 0.65 +0.3 811,738 - COO64_RS06580 0.66 +1.5 811,738 - COO64_RS06580 0.66 -0.1 811,755 + COO64_RS06580 0.76 -0.1 811,755 + COO64_RS06580 0.76 +0.3 811,755 + COO64_RS06580 0.76 -0.2 811,756 - COO64_RS06580 0.77 -0.4 811,756 - COO64_RS06580 0.77 -0.8 811,827 - +0.4 811,832 + +0.7 811,833 - -1.0 811,833 - +0.8 811,892 + +0.9 811,892 + +1.2 811,892 + +1.3 811,892 + +0.4 811,892 + -0.7 811,892 + -1.4 811,892 + -1.7 811,892 + -0.4 811,892 + +1.1 811,893 - -0.3 811,893 - -1.0 811,893 - -0.7 811,893 - +0.9 811,893 - -0.3 811,893 - +0.6 811,893 - -0.7 811,893 - -1.3 811,893 - +0.1 811,893 - -1.6 811,984 + COO64_RS06585 0.20 +0.1 811,985 - COO64_RS06585 0.20 -0.5 812,018 - COO64_RS06585 0.24 +0.8 812,018 - COO64_RS06585 0.24 -0.3 812,018 - COO64_RS06585 0.24 -0.0 812,018 - COO64_RS06585 0.24 -0.5 812,018 - COO64_RS06585 0.24 +0.5 812,050 - COO64_RS06585 0.28 -0.3 812,065 + COO64_RS06585 0.30 +0.6 812,083 + COO64_RS06585 0.32 +0.0 812,091 + COO64_RS06585 0.33 -0.1 812,091 + COO64_RS06585 0.33 +0.1 812,135 + COO64_RS06585 0.39 -2.3 812,135 + COO64_RS06585 0.39 -0.0 812,135 + COO64_RS06585 0.39 -0.2 812,135 + COO64_RS06585 0.39 -0.1 812,136 - COO64_RS06585 0.39 +0.7 812,136 - COO64_RS06585 0.39 +0.8 812,136 - COO64_RS06585 0.39 +0.3 812,136 - COO64_RS06585 0.39 +0.9 812,136 - COO64_RS06585 0.39 -0.5 812,136 - COO64_RS06585 0.39 -0.3 812,136 - COO64_RS06585 0.39 -0.7 812,136 - COO64_RS06585 0.39 +0.7 812,136 - COO64_RS06585 0.39 -0.2 812,186 - COO64_RS06585 0.45 +0.7 812,186 - COO64_RS06585 0.45 +0.8 812,209 + COO64_RS06585 0.48 +0.3 812,209 + COO64_RS06585 0.48 -0.9 812,209 + COO64_RS06585 0.48 -1.3 812,210 - COO64_RS06585 0.48 +0.6 812,210 - COO64_RS06585 0.48 -1.3 812,210 - COO64_RS06585 0.48 +0.3 812,210 - COO64_RS06585 0.48 +0.6 812,274 + COO64_RS06585 0.56 -1.0 812,354 - COO64_RS06585 0.67 -0.8 812,367 + COO64_RS06585 0.68 +1.1
Or see this region's nucleotide sequence