Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prmB and COO64_RS06260 are separated by 189 nucleotides COO64_RS06260 and COO64_RS06265 are separated by 76 nucleotides COO64_RS06265 and COO64_RS06270 are separated by 52 nucleotides
COO64_RS06255: prmB - 50S ribosomal protein L3 N(5)-glutamine methyltransferase, at 760,213 to 761,121
prmB
COO64_RS06260: COO64_RS06260 - cysteine hydrolase family protein, at 761,311 to 761,901
_RS06260
COO64_RS06265: COO64_RS06265 - hypothetical protein, at 761,978 to 762,298
_RS06265
COO64_RS06270: COO64_RS06270 - Smr/MutS family protein, at 762,351 to 762,908
_RS06270
Position (kb) 761
762 Strain fitness (log2 ratio) -2
-1
0
1 at 760.325 kb on + strand, within prmB at 760.326 kb on - strand, within prmB at 760.475 kb on + strand, within prmB at 760.475 kb on + strand, within prmB at 760.475 kb on + strand, within prmB at 760.475 kb on + strand, within prmB at 760.476 kb on - strand, within prmB at 760.476 kb on - strand, within prmB at 760.514 kb on + strand, within prmB at 760.514 kb on + strand, within prmB at 760.514 kb on + strand, within prmB at 760.584 kb on + strand, within prmB at 760.585 kb on - strand, within prmB at 760.586 kb on + strand, within prmB at 760.586 kb on + strand, within prmB at 760.586 kb on + strand, within prmB at 760.586 kb on + strand, within prmB at 760.587 kb on - strand, within prmB at 760.587 kb on - strand, within prmB at 760.587 kb on - strand, within prmB at 760.587 kb on - strand, within prmB at 760.587 kb on - strand, within prmB at 760.587 kb on - strand, within prmB at 760.868 kb on + strand, within prmB at 760.868 kb on + strand, within prmB at 760.955 kb on + strand, within prmB at 760.955 kb on + strand, within prmB at 760.956 kb on - strand, within prmB at 760.956 kb on - strand, within prmB at 761.153 kb on + strand at 761.193 kb on + strand at 761.193 kb on + strand at 761.194 kb on - strand at 761.194 kb on - strand at 761.194 kb on - strand at 761.194 kb on - strand at 761.194 kb on - strand at 761.239 kb on + strand at 761.257 kb on + strand at 761.257 kb on + strand at 761.257 kb on + strand at 761.257 kb on + strand at 761.257 kb on + strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.258 kb on - strand at 761.279 kb on + strand at 761.279 kb on + strand at 761.280 kb on - strand at 761.280 kb on - strand at 761.285 kb on - strand at 761.285 kb on - strand at 761.285 kb on - strand at 761.410 kb on + strand, within COO64_RS06260 at 761.411 kb on - strand, within COO64_RS06260 at 761.466 kb on + strand, within COO64_RS06260 at 761.467 kb on - strand, within COO64_RS06260 at 761.669 kb on - strand, within COO64_RS06260 at 761.669 kb on - strand, within COO64_RS06260 at 761.669 kb on - strand, within COO64_RS06260 at 761.746 kb on + strand, within COO64_RS06260 at 761.877 kb on - strand at 761.964 kb on + strand at 761.986 kb on - strand at 762.012 kb on + strand, within COO64_RS06265 at 762.013 kb on - strand, within COO64_RS06265 at 762.203 kb on + strand, within COO64_RS06265 at 762.327 kb on + strand at 762.348 kb on + strand at 762.348 kb on + strand at 762.348 kb on + strand at 762.348 kb on + strand at 762.348 kb on + strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.349 kb on - strand at 762.432 kb on - strand, within COO64_RS06270 at 762.825 kb on - strand, within COO64_RS06270 at 762.825 kb on - strand, within COO64_RS06270 at 762.851 kb on + strand, within COO64_RS06270 at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.864 kb on + strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand at 762.865 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 760,325 + prmB COO64_RS06255 0.12 +1.0 760,326 - prmB COO64_RS06255 0.12 -0.2 760,475 + prmB COO64_RS06255 0.29 -1.2 760,475 + prmB COO64_RS06255 0.29 +0.4 760,475 + prmB COO64_RS06255 0.29 -0.6 760,475 + prmB COO64_RS06255 0.29 -0.1 760,476 - prmB COO64_RS06255 0.29 +0.3 760,476 - prmB COO64_RS06255 0.29 -0.7 760,514 + prmB COO64_RS06255 0.33 +0.9 760,514 + prmB COO64_RS06255 0.33 -0.5 760,514 + prmB COO64_RS06255 0.33 +0.2 760,584 + prmB COO64_RS06255 0.41 +0.3 760,585 - prmB COO64_RS06255 0.41 -0.8 760,586 + prmB COO64_RS06255 0.41 +0.4 760,586 + prmB COO64_RS06255 0.41 -0.6 760,586 + prmB COO64_RS06255 0.41 -0.6 760,586 + prmB COO64_RS06255 0.41 +0.3 760,587 - prmB COO64_RS06255 0.41 +0.5 760,587 - prmB COO64_RS06255 0.41 +0.7 760,587 - prmB COO64_RS06255 0.41 +0.0 760,587 - prmB COO64_RS06255 0.41 -1.1 760,587 - prmB COO64_RS06255 0.41 +1.4 760,587 - prmB COO64_RS06255 0.41 -0.4 760,868 + prmB COO64_RS06255 0.72 -0.3 760,868 + prmB COO64_RS06255 0.72 -0.1 760,955 + prmB COO64_RS06255 0.82 +0.7 760,955 + prmB COO64_RS06255 0.82 -0.4 760,956 - prmB COO64_RS06255 0.82 -0.8 760,956 - prmB COO64_RS06255 0.82 +0.4 761,153 + +0.1 761,193 + -0.1 761,193 + +0.7 761,194 - +0.0 761,194 - +0.6 761,194 - -0.8 761,194 - -0.1 761,194 - -0.1 761,239 + +0.1 761,257 + +1.0 761,257 + +0.2 761,257 + -0.7 761,257 + -0.6 761,257 + -0.7 761,258 - +0.1 761,258 - +0.9 761,258 - -1.2 761,258 - -0.2 761,258 - +0.6 761,258 - +0.2 761,258 - -0.3 761,258 - -0.3 761,279 + +0.2 761,279 + +0.3 761,280 - -1.1 761,280 - -0.9 761,285 - -0.7 761,285 - -1.0 761,285 - +0.7 761,410 + COO64_RS06260 0.17 -1.4 761,411 - COO64_RS06260 0.17 +1.1 761,466 + COO64_RS06260 0.26 -0.7 761,467 - COO64_RS06260 0.26 -1.0 761,669 - COO64_RS06260 0.61 -0.3 761,669 - COO64_RS06260 0.61 -0.2 761,669 - COO64_RS06260 0.61 -0.6 761,746 + COO64_RS06260 0.74 +0.4 761,877 - +1.0 761,964 + -0.0 761,986 - -1.1 762,012 + COO64_RS06265 0.11 -0.6 762,013 - COO64_RS06265 0.11 -0.2 762,203 + COO64_RS06265 0.70 -1.4 762,327 + -0.1 762,348 + -0.3 762,348 + -0.8 762,348 + -0.4 762,348 + -0.3 762,348 + -0.1 762,349 - -0.1 762,349 - +0.0 762,349 - -0.0 762,349 - -1.8 762,349 - +0.1 762,349 - +1.3 762,349 - +0.1 762,349 - -1.1 762,349 - +1.2 762,349 - +0.6 762,349 - -0.5 762,349 - -0.9 762,349 - -1.4 762,349 - +1.0 762,349 - -0.5 762,432 - COO64_RS06270 0.15 +0.7 762,825 - COO64_RS06270 0.85 +0.4 762,825 - COO64_RS06270 0.85 -0.2 762,851 + COO64_RS06270 0.90 -1.1 762,864 + +0.0 762,864 + +0.2 762,864 + -0.4 762,864 + -0.3 762,864 + -0.2 762,864 + -1.1 762,864 + -0.8 762,864 + +0.6 762,864 + +0.2 762,864 + +1.0 762,864 + -0.9 762,864 + -0.1 762,864 + -0.7 762,864 + -0.3 762,865 - +0.4 762,865 - -0.7 762,865 - +0.3 762,865 - -0.2 762,865 - +0.2 762,865 - +0.7 762,865 - +0.8 762,865 - -1.5 762,865 - -0.2
Or see this region's nucleotide sequence