Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt murB and COO64_RS05860 overlap by 4 nucleotides COO64_RS05860 and kdsB overlap by 1 nucleotides kdsB and COO64_RS05870 overlap by 4 nucleotides COO64_RS05870 and lpxK are separated by 37 nucleotides
COO64_RS05855: murB - UDP-N-acetylmuramate dehydrogenase, at 688,183 to 689,202
murB
COO64_RS05860: COO64_RS05860 - low molecular weight protein-tyrosine-phosphatase, at 689,199 to 689,663
_RS05860
COO64_RS05865: kdsB - 3-deoxy-manno-octulosonate cytidylyltransferase, at 689,663 to 690,427
kdsB
COO64_RS05870: COO64_RS05870 - Trm112 family protein, at 690,424 to 690,609
_RS05870
COO64_RS05875: lpxK - tetraacyldisaccharide 4'-kinase, at 690,647 to 691,657
lpxK
Position (kb) 689
690 Strain fitness (log2 ratio) -2
-1
0
1 at 689.177 kb on - strand at 689.203 kb on - strand at 689.288 kb on - strand, within COO64_RS05860 at 689.291 kb on - strand, within COO64_RS05860 at 689.291 kb on - strand, within COO64_RS05860 at 689.291 kb on - strand, within COO64_RS05860 at 689.291 kb on - strand, within COO64_RS05860 at 689.477 kb on - strand, within COO64_RS05860 at 689.485 kb on - strand, within COO64_RS05860 at 689.496 kb on - strand, within COO64_RS05860 at 689.634 kb on - strand at 689.634 kb on - strand at 689.634 kb on - strand at 689.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 689,177 - +1.7 689,203 - +0.9 689,288 - COO64_RS05860 0.19 +0.2 689,291 - COO64_RS05860 0.20 -0.3 689,291 - COO64_RS05860 0.20 -0.6 689,291 - COO64_RS05860 0.20 -0.8 689,291 - COO64_RS05860 0.20 -1.1 689,477 - COO64_RS05860 0.60 -1.0 689,485 - COO64_RS05860 0.62 -2.5 689,496 - COO64_RS05860 0.64 +0.2 689,634 - -0.4 689,634 - +0.3 689,634 - +0.2 689,634 - -2.3
Or see this region's nucleotide sequence