Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS05595 and purF are separated by 15 nucleotides purF and COO64_RS05605 are separated by 41 nucleotides COO64_RS05605 and COO64_RS05610 are separated by 99 nucleotides
COO64_RS05595: COO64_RS05595 - O-succinylhomoserine sulfhydrylase, at 634,606 to 635,817
_RS05595
COO64_RS05600: purF - amidophosphoribosyltransferase, at 635,833 to 637,338
purF
COO64_RS05605: COO64_RS05605 - CvpA family protein, at 637,380 to 637,934
_RS05605
COO64_RS05610: COO64_RS05610 - SPOR domain-containing protein, at 638,034 to 638,702
_RS05610
Position (kb) 635
636
637
638 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 634.928 kb on + strand, within COO64_RS05595 at 635.394 kb on - strand, within COO64_RS05595 at 635.423 kb on + strand, within COO64_RS05595 at 635.423 kb on + strand, within COO64_RS05595 at 635.423 kb on + strand, within COO64_RS05595 at 635.423 kb on + strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.424 kb on - strand, within COO64_RS05595 at 635.609 kb on + strand, within COO64_RS05595 at 635.610 kb on - strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.618 kb on + strand, within COO64_RS05595 at 635.619 kb on - strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.681 kb on + strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.682 kb on - strand, within COO64_RS05595 at 635.829 kb on + strand at 635.829 kb on + strand at 635.829 kb on + strand at 635.829 kb on + strand at 635.830 kb on - strand at 635.830 kb on - strand at 635.830 kb on - strand at 635.830 kb on - strand at 635.830 kb on - strand at 635.834 kb on + strand at 635.834 kb on + strand at 635.834 kb on + strand at 635.834 kb on + strand at 635.834 kb on + strand at 635.834 kb on + strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.835 kb on - strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.843 kb on + strand at 635.844 kb on - strand at 635.844 kb on - strand at 635.921 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.948 kb on + strand at 635.949 kb on - strand at 635.949 kb on - strand at 635.949 kb on - strand at 635.949 kb on - strand at 635.949 kb on - strand at 635.949 kb on - strand at 636.035 kb on + strand, within purF at 636.036 kb on - strand, within purF at 636.087 kb on + strand, within purF at 636.170 kb on + strand, within purF at 636.345 kb on + strand, within purF at 636.346 kb on - strand, within purF at 636.356 kb on + strand, within purF at 636.356 kb on + strand, within purF at 636.356 kb on + strand, within purF at 636.357 kb on - strand, within purF at 636.357 kb on - strand, within purF at 636.357 kb on - strand, within purF at 636.527 kb on + strand, within purF at 636.527 kb on + strand, within purF at 636.528 kb on - strand, within purF at 636.569 kb on + strand, within purF at 636.569 kb on + strand, within purF at 636.570 kb on - strand, within purF at 636.570 kb on - strand, within purF at 636.650 kb on + strand, within purF at 636.650 kb on + strand, within purF at 636.650 kb on + strand, within purF at 636.650 kb on + strand, within purF at 636.650 kb on + strand, within purF at 636.650 kb on + strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.651 kb on - strand, within purF at 636.731 kb on + strand, within purF at 636.731 kb on + strand, within purF at 636.731 kb on + strand, within purF at 636.731 kb on + strand, within purF at 636.732 kb on - strand, within purF at 636.732 kb on - strand, within purF at 636.732 kb on - strand, within purF at 636.883 kb on + strand, within purF at 636.883 kb on + strand, within purF at 636.883 kb on + strand, within purF at 636.884 kb on - strand, within purF at 636.884 kb on - strand, within purF at 636.995 kb on + strand, within purF at 636.995 kb on + strand, within purF at 636.995 kb on + strand, within purF at 636.995 kb on + strand, within purF at 636.996 kb on - strand, within purF at 636.996 kb on - strand, within purF at 636.996 kb on - strand, within purF at 637.073 kb on + strand, within purF at 637.073 kb on + strand, within purF at 637.073 kb on + strand, within purF at 637.074 kb on - strand, within purF at 637.074 kb on - strand, within purF at 637.074 kb on - strand, within purF at 637.134 kb on + strand, within purF at 637.135 kb on - strand, within purF at 637.135 kb on - strand, within purF at 637.161 kb on + strand, within purF at 637.161 kb on + strand, within purF at 637.161 kb on + strand, within purF at 637.162 kb on - strand, within purF at 637.162 kb on - strand, within purF at 637.162 kb on - strand, within purF at 637.240 kb on - strand at 637.286 kb on + strand at 637.286 kb on + strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.287 kb on - strand at 637.312 kb on - strand at 637.352 kb on + strand at 637.352 kb on + strand at 637.353 kb on - strand at 637.356 kb on + strand at 637.356 kb on + strand at 637.357 kb on - strand at 637.497 kb on + strand, within COO64_RS05605 at 637.498 kb on - strand, within COO64_RS05605 at 637.504 kb on - strand, within COO64_RS05605 at 637.504 kb on - strand, within COO64_RS05605 at 637.552 kb on - strand, within COO64_RS05605 at 637.552 kb on - strand, within COO64_RS05605 at 637.552 kb on - strand, within COO64_RS05605 at 637.585 kb on - strand, within COO64_RS05605 at 637.763 kb on + strand, within COO64_RS05605 at 637.764 kb on - strand, within COO64_RS05605 at 637.765 kb on + strand, within COO64_RS05605 at 637.765 kb on + strand, within COO64_RS05605 at 637.765 kb on + strand, within COO64_RS05605 at 637.765 kb on + strand, within COO64_RS05605 at 637.765 kb on + strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.766 kb on - strand, within COO64_RS05605 at 637.778 kb on - strand, within COO64_RS05605 at 637.778 kb on - strand, within COO64_RS05605 at 637.832 kb on + strand, within COO64_RS05605 at 637.832 kb on + strand, within COO64_RS05605 at 637.832 kb on + strand, within COO64_RS05605 at 637.833 kb on - strand, within COO64_RS05605 at 637.925 kb on + strand at 637.925 kb on + strand at 637.925 kb on + strand at 637.926 kb on - strand at 637.926 kb on - strand at 637.926 kb on - strand at 637.926 kb on - strand at 637.969 kb on + strand at 638.003 kb on + strand at 638.058 kb on + strand at 638.143 kb on + strand, within COO64_RS05610 at 638.143 kb on + strand, within COO64_RS05610 at 638.143 kb on + strand, within COO64_RS05610 at 638.143 kb on + strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.144 kb on - strand, within COO64_RS05610 at 638.176 kb on + strand, within COO64_RS05610 at 638.176 kb on + strand, within COO64_RS05610 at 638.176 kb on + strand, within COO64_RS05610 at 638.177 kb on - strand, within COO64_RS05610 at 638.185 kb on + strand, within COO64_RS05610 at 638.185 kb on + strand, within COO64_RS05610 at 638.185 kb on + strand, within COO64_RS05610 at 638.185 kb on + strand, within COO64_RS05610 at 638.186 kb on - strand, within COO64_RS05610
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 634,928 + COO64_RS05595 0.27 -0.7 635,394 - COO64_RS05595 0.65 -0.4 635,423 + COO64_RS05595 0.67 +0.7 635,423 + COO64_RS05595 0.67 +0.3 635,423 + COO64_RS05595 0.67 -0.6 635,423 + COO64_RS05595 0.67 +0.8 635,424 - COO64_RS05595 0.67 +0.7 635,424 - COO64_RS05595 0.67 +0.8 635,424 - COO64_RS05595 0.67 +0.2 635,424 - COO64_RS05595 0.67 +1.3 635,424 - COO64_RS05595 0.67 +0.1 635,424 - COO64_RS05595 0.67 -0.6 635,609 + COO64_RS05595 0.83 +1.0 635,610 - COO64_RS05595 0.83 -0.0 635,618 + COO64_RS05595 0.83 -0.2 635,618 + COO64_RS05595 0.83 +0.1 635,618 + COO64_RS05595 0.83 +0.4 635,618 + COO64_RS05595 0.83 +0.2 635,618 + COO64_RS05595 0.83 -1.6 635,618 + COO64_RS05595 0.83 +0.8 635,618 + COO64_RS05595 0.83 +0.8 635,619 - COO64_RS05595 0.84 -0.0 635,681 + COO64_RS05595 0.89 +0.1 635,681 + COO64_RS05595 0.89 -1.0 635,681 + COO64_RS05595 0.89 -0.7 635,681 + COO64_RS05595 0.89 +0.2 635,681 + COO64_RS05595 0.89 -0.9 635,681 + COO64_RS05595 0.89 +0.3 635,681 + COO64_RS05595 0.89 +0.3 635,681 + COO64_RS05595 0.89 -0.5 635,681 + COO64_RS05595 0.89 -0.8 635,681 + COO64_RS05595 0.89 -0.7 635,682 - COO64_RS05595 0.89 -1.3 635,682 - COO64_RS05595 0.89 -0.2 635,682 - COO64_RS05595 0.89 +1.0 635,682 - COO64_RS05595 0.89 -0.1 635,682 - COO64_RS05595 0.89 -0.8 635,682 - COO64_RS05595 0.89 +0.2 635,682 - COO64_RS05595 0.89 +0.0 635,682 - COO64_RS05595 0.89 -0.3 635,682 - COO64_RS05595 0.89 +0.1 635,682 - COO64_RS05595 0.89 +0.1 635,682 - COO64_RS05595 0.89 +0.0 635,829 + -1.7 635,829 + +0.1 635,829 + +0.3 635,829 + -1.4 635,830 - -2.9 635,830 - +1.1 635,830 - +0.7 635,830 - +0.1 635,830 - -0.2 635,834 + +0.1 635,834 + -0.5 635,834 + -1.0 635,834 + -0.0 635,834 + +0.8 635,834 + +0.9 635,835 - +0.0 635,835 - -2.0 635,835 - +0.5 635,835 - +0.2 635,835 - -0.6 635,835 - -1.3 635,835 - +0.1 635,843 + +0.1 635,843 + -0.2 635,843 + +0.6 635,843 + -0.4 635,843 + -0.6 635,843 + -0.1 635,843 + +0.4 635,843 + -0.7 635,844 - +1.2 635,844 - +0.4 635,921 + -0.3 635,948 + -1.5 635,948 + +0.3 635,948 + -0.1 635,948 + +0.6 635,948 + -0.4 635,948 + -1.5 635,948 + -1.6 635,948 + -0.4 635,948 + +0.3 635,948 + -1.6 635,949 - -2.7 635,949 - -0.7 635,949 - -0.5 635,949 - +0.1 635,949 - +0.3 635,949 - -1.5 636,035 + purF COO64_RS05600 0.13 +0.3 636,036 - purF COO64_RS05600 0.13 -1.2 636,087 + purF COO64_RS05600 0.17 -1.4 636,170 + purF COO64_RS05600 0.22 -1.7 636,345 + purF COO64_RS05600 0.34 -1.6 636,346 - purF COO64_RS05600 0.34 -2.2 636,356 + purF COO64_RS05600 0.35 -0.1 636,356 + purF COO64_RS05600 0.35 -1.1 636,356 + purF COO64_RS05600 0.35 -0.2 636,357 - purF COO64_RS05600 0.35 +0.3 636,357 - purF COO64_RS05600 0.35 -1.7 636,357 - purF COO64_RS05600 0.35 +1.2 636,527 + purF COO64_RS05600 0.46 -1.6 636,527 + purF COO64_RS05600 0.46 -0.2 636,528 - purF COO64_RS05600 0.46 -0.9 636,569 + purF COO64_RS05600 0.49 -0.8 636,569 + purF COO64_RS05600 0.49 -1.9 636,570 - purF COO64_RS05600 0.49 -0.7 636,570 - purF COO64_RS05600 0.49 -0.8 636,650 + purF COO64_RS05600 0.54 -2.3 636,650 + purF COO64_RS05600 0.54 +0.6 636,650 + purF COO64_RS05600 0.54 -0.1 636,650 + purF COO64_RS05600 0.54 +0.6 636,650 + purF COO64_RS05600 0.54 -1.4 636,650 + purF COO64_RS05600 0.54 -1.3 636,651 - purF COO64_RS05600 0.54 -0.9 636,651 - purF COO64_RS05600 0.54 -1.2 636,651 - purF COO64_RS05600 0.54 -1.3 636,651 - purF COO64_RS05600 0.54 -0.7 636,651 - purF COO64_RS05600 0.54 -0.4 636,651 - purF COO64_RS05600 0.54 -1.1 636,651 - purF COO64_RS05600 0.54 -1.7 636,651 - purF COO64_RS05600 0.54 -0.4 636,651 - purF COO64_RS05600 0.54 -1.5 636,731 + purF COO64_RS05600 0.60 -2.2 636,731 + purF COO64_RS05600 0.60 -1.4 636,731 + purF COO64_RS05600 0.60 -1.0 636,731 + purF COO64_RS05600 0.60 -0.4 636,732 - purF COO64_RS05600 0.60 -2.6 636,732 - purF COO64_RS05600 0.60 -1.4 636,732 - purF COO64_RS05600 0.60 -0.7 636,883 + purF COO64_RS05600 0.70 -0.5 636,883 + purF COO64_RS05600 0.70 -1.1 636,883 + purF COO64_RS05600 0.70 -1.4 636,884 - purF COO64_RS05600 0.70 -1.6 636,884 - purF COO64_RS05600 0.70 -1.2 636,995 + purF COO64_RS05600 0.77 -1.8 636,995 + purF COO64_RS05600 0.77 -1.4 636,995 + purF COO64_RS05600 0.77 -1.9 636,995 + purF COO64_RS05600 0.77 -2.0 636,996 - purF COO64_RS05600 0.77 +0.6 636,996 - purF COO64_RS05600 0.77 -0.5 636,996 - purF COO64_RS05600 0.77 -0.9 637,073 + purF COO64_RS05600 0.82 -1.3 637,073 + purF COO64_RS05600 0.82 +0.4 637,073 + purF COO64_RS05600 0.82 +0.6 637,074 - purF COO64_RS05600 0.82 -0.9 637,074 - purF COO64_RS05600 0.82 -1.6 637,074 - purF COO64_RS05600 0.82 -2.4 637,134 + purF COO64_RS05600 0.86 -1.7 637,135 - purF COO64_RS05600 0.86 -1.5 637,135 - purF COO64_RS05600 0.86 -0.4 637,161 + purF COO64_RS05600 0.88 -1.3 637,161 + purF COO64_RS05600 0.88 -0.9 637,161 + purF COO64_RS05600 0.88 -1.7 637,162 - purF COO64_RS05600 0.88 +1.5 637,162 - purF COO64_RS05600 0.88 -2.9 637,162 - purF COO64_RS05600 0.88 -0.4 637,240 - -1.4 637,286 + +0.3 637,286 + +0.3 637,287 - +0.3 637,287 - -1.7 637,287 - -2.5 637,287 - -1.3 637,287 - -0.4 637,287 - -0.4 637,287 - -0.2 637,287 - -0.7 637,312 - -2.6 637,352 + -0.3 637,352 + -2.0 637,353 - -0.5 637,356 + -2.6 637,356 + -0.4 637,357 - -0.4 637,497 + COO64_RS05605 0.21 -0.3 637,498 - COO64_RS05605 0.21 +0.4 637,504 - COO64_RS05605 0.22 +0.5 637,504 - COO64_RS05605 0.22 -1.6 637,552 - COO64_RS05605 0.31 +0.6 637,552 - COO64_RS05605 0.31 -1.3 637,552 - COO64_RS05605 0.31 +1.0 637,585 - COO64_RS05605 0.37 -0.1 637,763 + COO64_RS05605 0.69 -2.1 637,764 - COO64_RS05605 0.69 +1.1 637,765 + COO64_RS05605 0.69 -0.4 637,765 + COO64_RS05605 0.69 +0.3 637,765 + COO64_RS05605 0.69 +0.9 637,765 + COO64_RS05605 0.69 -0.2 637,765 + COO64_RS05605 0.69 +0.2 637,766 - COO64_RS05605 0.70 -0.1 637,766 - COO64_RS05605 0.70 +1.9 637,766 - COO64_RS05605 0.70 +0.6 637,766 - COO64_RS05605 0.70 +1.0 637,766 - COO64_RS05605 0.70 -0.4 637,766 - COO64_RS05605 0.70 -0.0 637,766 - COO64_RS05605 0.70 -0.0 637,778 - COO64_RS05605 0.72 +0.7 637,778 - COO64_RS05605 0.72 +0.0 637,832 + COO64_RS05605 0.81 -0.0 637,832 + COO64_RS05605 0.81 +0.4 637,832 + COO64_RS05605 0.81 +1.4 637,833 - COO64_RS05605 0.82 +0.3 637,925 + -0.3 637,925 + +0.9 637,925 + -1.6 637,926 - +0.3 637,926 - +1.1 637,926 - +1.0 637,926 - +0.6 637,969 + -0.4 638,003 + +1.0 638,058 + -0.4 638,143 + COO64_RS05610 0.16 +0.4 638,143 + COO64_RS05610 0.16 -0.1 638,143 + COO64_RS05610 0.16 +0.8 638,143 + COO64_RS05610 0.16 -1.1 638,144 - COO64_RS05610 0.16 +0.9 638,144 - COO64_RS05610 0.16 -0.3 638,144 - COO64_RS05610 0.16 -0.5 638,144 - COO64_RS05610 0.16 -0.8 638,144 - COO64_RS05610 0.16 -0.1 638,144 - COO64_RS05610 0.16 +0.6 638,144 - COO64_RS05610 0.16 +0.7 638,176 + COO64_RS05610 0.21 +1.6 638,176 + COO64_RS05610 0.21 +0.4 638,176 + COO64_RS05610 0.21 +0.4 638,177 - COO64_RS05610 0.21 -0.2 638,185 + COO64_RS05610 0.23 +0.2 638,185 + COO64_RS05610 0.23 +1.4 638,185 + COO64_RS05610 0.23 +0.8 638,185 + COO64_RS05610 0.23 +0.4 638,186 - COO64_RS05610 0.23 -0.1
Or see this region's nucleotide sequence