Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sigX and COO64_RS05355 are separated by 114 nucleotides COO64_RS05355 and COO64_RS05360 are separated by 2 nucleotides COO64_RS05360 and COO64_RS05365 are separated by 99 nucleotides
COO64_RS05350: sigX - RNA polymerase sigma factor SigX, at 578,674 to 579,240
sigX
COO64_RS05355: COO64_RS05355 - mechanosensitive ion channel family protein, at 579,355 to 580,179
_RS05355
COO64_RS05360: COO64_RS05360 - CrfX protein, at 580,182 to 580,430
_RS05360
COO64_RS05365: COO64_RS05365 - zinc transporter ZntB, at 580,530 to 581,525
_RS05365
Position (kb)
580
581 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 579.234 kb on - strand at 579.258 kb on + strand at 579.302 kb on + strand at 579.333 kb on + strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.339 kb on - strand at 579.339 kb on - strand at 579.363 kb on + strand at 579.444 kb on + strand, within COO64_RS05355 at 579.503 kb on + strand, within COO64_RS05355 at 579.696 kb on + strand, within COO64_RS05355 at 579.716 kb on + strand, within COO64_RS05355 at 579.716 kb on + strand, within COO64_RS05355 at 579.717 kb on - strand, within COO64_RS05355 at 579.717 kb on - strand, within COO64_RS05355 at 579.778 kb on + strand, within COO64_RS05355 at 579.778 kb on + strand, within COO64_RS05355 at 579.778 kb on + strand, within COO64_RS05355 at 579.778 kb on + strand, within COO64_RS05355 at 579.824 kb on + strand, within COO64_RS05355 at 579.915 kb on - strand, within COO64_RS05355 at 579.915 kb on - strand, within COO64_RS05355 at 580.179 kb on + strand at 580.218 kb on - strand, within COO64_RS05360 at 580.430 kb on + strand at 580.446 kb on + strand at 580.446 kb on + strand at 580.447 kb on - strand at 580.711 kb on + strand, within COO64_RS05365 at 580.711 kb on + strand, within COO64_RS05365 at 580.711 kb on + strand, within COO64_RS05365 at 580.711 kb on + strand, within COO64_RS05365 at 580.712 kb on - strand, within COO64_RS05365 at 580.712 kb on - strand, within COO64_RS05365 at 580.712 kb on - strand, within COO64_RS05365 at 580.838 kb on - strand, within COO64_RS05365 at 580.891 kb on + strand, within COO64_RS05365 at 580.975 kb on + strand, within COO64_RS05365 at 580.976 kb on - strand, within COO64_RS05365 at 580.976 kb on - strand, within COO64_RS05365 at 581.082 kb on + strand, within COO64_RS05365 at 581.082 kb on + strand, within COO64_RS05365 at 581.082 kb on + strand, within COO64_RS05365 at 581.083 kb on - strand, within COO64_RS05365
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 579,234 - +0.3 579,258 + -0.7 579,302 + +0.9 579,333 + -0.9 579,334 - -0.2 579,334 - -0.1 579,334 - +1.7 579,334 - -1.3 579,334 - +1.8 579,334 - -0.7 579,334 - -0.7 579,339 - -1.4 579,339 - +0.3 579,363 + +0.3 579,444 + COO64_RS05355 0.11 -0.2 579,503 + COO64_RS05355 0.18 +0.7 579,696 + COO64_RS05355 0.41 +0.9 579,716 + COO64_RS05355 0.44 +0.6 579,716 + COO64_RS05355 0.44 +0.1 579,717 - COO64_RS05355 0.44 -1.2 579,717 - COO64_RS05355 0.44 +0.5 579,778 + COO64_RS05355 0.51 -0.6 579,778 + COO64_RS05355 0.51 -0.3 579,778 + COO64_RS05355 0.51 -1.2 579,778 + COO64_RS05355 0.51 -0.5 579,824 + COO64_RS05355 0.57 -0.4 579,915 - COO64_RS05355 0.68 +0.3 579,915 - COO64_RS05355 0.68 +0.5 580,179 + -1.4 580,218 - COO64_RS05360 0.14 -0.7 580,430 + -0.7 580,446 + +0.5 580,446 + -2.2 580,447 - -0.4 580,711 + COO64_RS05365 0.18 -0.1 580,711 + COO64_RS05365 0.18 +0.3 580,711 + COO64_RS05365 0.18 +0.5 580,711 + COO64_RS05365 0.18 -0.7 580,712 - COO64_RS05365 0.18 -0.2 580,712 - COO64_RS05365 0.18 -0.5 580,712 - COO64_RS05365 0.18 +0.1 580,838 - COO64_RS05365 0.31 +0.6 580,891 + COO64_RS05365 0.36 +0.4 580,975 + COO64_RS05365 0.45 -0.3 580,976 - COO64_RS05365 0.45 +0.1 580,976 - COO64_RS05365 0.45 -0.8 581,082 + COO64_RS05365 0.55 -0.1 581,082 + COO64_RS05365 0.55 -1.4 581,082 + COO64_RS05365 0.55 +0.2 581,083 - COO64_RS05365 0.56 -0.1
Or see this region's nucleotide sequence