Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS05340 and COO64_RS05345 are separated by 64 nucleotides COO64_RS05345 and sigX are separated by 110 nucleotides sigX and COO64_RS05355 are separated by 114 nucleotides
COO64_RS05340: COO64_RS05340 - tryptophan 2-monooxygenase oxidoreductase, at 575,338 to 577,461
_RS05340
COO64_RS05345: COO64_RS05345 - OmpA family protein, at 577,526 to 578,563
_RS05345
COO64_RS05350: sigX - RNA polymerase sigma factor SigX, at 578,674 to 579,240
sigX
COO64_RS05355: COO64_RS05355 - mechanosensitive ion channel family protein, at 579,355 to 580,179
_RS05355
Position (kb) 577
578
579 Strain fitness (log2 ratio) -2
-1
0
1
2 at 576.559 kb on + strand, within COO64_RS05340 at 576.559 kb on + strand, within COO64_RS05340 at 576.559 kb on + strand, within COO64_RS05340 at 576.559 kb on + strand, within COO64_RS05340 at 576.559 kb on + strand, within COO64_RS05340 at 576.559 kb on + strand, within COO64_RS05340 at 576.560 kb on - strand, within COO64_RS05340 at 576.560 kb on - strand, within COO64_RS05340 at 576.560 kb on - strand, within COO64_RS05340 at 576.560 kb on - strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.565 kb on + strand, within COO64_RS05340 at 576.566 kb on - strand, within COO64_RS05340 at 576.566 kb on - strand, within COO64_RS05340 at 576.566 kb on - strand, within COO64_RS05340 at 576.566 kb on - strand, within COO64_RS05340 at 576.566 kb on - strand, within COO64_RS05340 at 576.729 kb on + strand, within COO64_RS05340 at 576.729 kb on + strand, within COO64_RS05340 at 576.795 kb on - strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.898 kb on + strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.899 kb on - strand, within COO64_RS05340 at 576.931 kb on + strand, within COO64_RS05340 at 576.931 kb on + strand, within COO64_RS05340 at 576.931 kb on + strand, within COO64_RS05340 at 576.952 kb on + strand, within COO64_RS05340 at 576.952 kb on + strand, within COO64_RS05340 at 576.952 kb on + strand, within COO64_RS05340 at 576.953 kb on - strand, within COO64_RS05340 at 576.991 kb on + strand, within COO64_RS05340 at 576.991 kb on + strand, within COO64_RS05340 at 576.991 kb on + strand, within COO64_RS05340 at 576.992 kb on - strand, within COO64_RS05340 at 576.992 kb on - strand, within COO64_RS05340 at 576.992 kb on - strand, within COO64_RS05340 at 576.992 kb on - strand, within COO64_RS05340 at 576.992 kb on - strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.045 kb on + strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.046 kb on - strand, within COO64_RS05340 at 577.087 kb on + strand, within COO64_RS05340 at 577.108 kb on + strand, within COO64_RS05340 at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.312 kb on + strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.313 kb on - strand at 577.495 kb on - strand at 577.511 kb on + strand at 577.527 kb on + strand at 577.527 kb on + strand at 577.528 kb on - strand at 577.528 kb on - strand at 577.545 kb on - strand at 577.545 kb on - strand at 577.545 kb on - strand at 577.545 kb on - strand at 577.545 kb on - strand at 577.545 kb on - strand at 577.621 kb on - strand at 577.656 kb on + strand, within COO64_RS05345 at 577.716 kb on + strand, within COO64_RS05345 at 577.717 kb on - strand, within COO64_RS05345 at 577.717 kb on - strand, within COO64_RS05345 at 577.773 kb on + strand, within COO64_RS05345 at 577.872 kb on - strand, within COO64_RS05345 at 577.872 kb on - strand, within COO64_RS05345 at 577.872 kb on - strand, within COO64_RS05345 at 577.872 kb on - strand, within COO64_RS05345 at 578.091 kb on + strand, within COO64_RS05345 at 578.091 kb on + strand, within COO64_RS05345 at 578.091 kb on + strand, within COO64_RS05345 at 578.091 kb on + strand, within COO64_RS05345 at 578.091 kb on + strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.092 kb on - strand, within COO64_RS05345 at 578.136 kb on + strand, within COO64_RS05345 at 578.136 kb on + strand, within COO64_RS05345 at 578.136 kb on + strand, within COO64_RS05345 at 578.137 kb on - strand, within COO64_RS05345 at 578.137 kb on - strand, within COO64_RS05345 at 578.137 kb on - strand, within COO64_RS05345 at 578.137 kb on - strand, within COO64_RS05345 at 578.235 kb on + strand, within COO64_RS05345 at 578.236 kb on - strand, within COO64_RS05345 at 578.271 kb on + strand, within COO64_RS05345 at 578.271 kb on + strand, within COO64_RS05345 at 578.271 kb on + strand, within COO64_RS05345 at 578.272 kb on - strand, within COO64_RS05345 at 578.385 kb on + strand, within COO64_RS05345 at 578.385 kb on + strand, within COO64_RS05345 at 578.386 kb on - strand, within COO64_RS05345 at 578.442 kb on + strand, within COO64_RS05345 at 578.442 kb on + strand, within COO64_RS05345 at 578.442 kb on + strand, within COO64_RS05345 at 578.443 kb on - strand, within COO64_RS05345 at 578.443 kb on - strand, within COO64_RS05345 at 578.443 kb on - strand, within COO64_RS05345 at 578.446 kb on - strand, within COO64_RS05345 at 578.446 kb on - strand, within COO64_RS05345 at 578.520 kb on - strand at 578.568 kb on + strand at 578.586 kb on + strand at 578.586 kb on + strand at 578.586 kb on + strand at 578.587 kb on - strand at 578.587 kb on - strand at 578.587 kb on - strand at 578.587 kb on - strand at 578.587 kb on - strand at 578.590 kb on + strand at 578.590 kb on + strand at 578.590 kb on + strand at 578.590 kb on + strand at 578.591 kb on - strand at 578.591 kb on - strand at 578.591 kb on - strand at 578.591 kb on - strand at 578.634 kb on + strand at 578.655 kb on + strand at 578.655 kb on + strand at 578.661 kb on + strand at 578.661 kb on + strand at 578.661 kb on + strand at 578.662 kb on - strand at 578.662 kb on - strand at 578.662 kb on - strand at 578.662 kb on - strand at 578.676 kb on - strand at 578.708 kb on - strand at 578.846 kb on - strand, within sigX at 578.984 kb on + strand, within sigX at 579.234 kb on - strand at 579.258 kb on + strand at 579.302 kb on + strand at 579.333 kb on + strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.334 kb on - strand at 579.339 kb on - strand at 579.339 kb on - strand at 579.363 kb on + strand at 579.444 kb on + strand, within COO64_RS05355 at 579.503 kb on + strand, within COO64_RS05355
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 576,559 + COO64_RS05340 0.57 +1.5 576,559 + COO64_RS05340 0.57 +0.2 576,559 + COO64_RS05340 0.57 +0.8 576,559 + COO64_RS05340 0.57 +0.7 576,559 + COO64_RS05340 0.57 -0.4 576,559 + COO64_RS05340 0.57 -0.5 576,560 - COO64_RS05340 0.58 +0.2 576,560 - COO64_RS05340 0.58 +1.3 576,560 - COO64_RS05340 0.58 -0.1 576,560 - COO64_RS05340 0.58 +0.4 576,565 + COO64_RS05340 0.58 +0.5 576,565 + COO64_RS05340 0.58 -0.2 576,565 + COO64_RS05340 0.58 +0.2 576,565 + COO64_RS05340 0.58 +0.2 576,565 + COO64_RS05340 0.58 +0.1 576,565 + COO64_RS05340 0.58 -0.4 576,565 + COO64_RS05340 0.58 -0.5 576,565 + COO64_RS05340 0.58 +1.0 576,566 - COO64_RS05340 0.58 +0.6 576,566 - COO64_RS05340 0.58 +0.3 576,566 - COO64_RS05340 0.58 +1.5 576,566 - COO64_RS05340 0.58 +1.2 576,566 - COO64_RS05340 0.58 -0.6 576,729 + COO64_RS05340 0.65 -0.1 576,729 + COO64_RS05340 0.65 +1.0 576,795 - COO64_RS05340 0.69 -0.0 576,898 + COO64_RS05340 0.73 -0.8 576,898 + COO64_RS05340 0.73 +0.3 576,898 + COO64_RS05340 0.73 -0.1 576,898 + COO64_RS05340 0.73 -0.3 576,898 + COO64_RS05340 0.73 +0.4 576,898 + COO64_RS05340 0.73 +0.1 576,898 + COO64_RS05340 0.73 +0.4 576,899 - COO64_RS05340 0.73 +0.2 576,899 - COO64_RS05340 0.73 +0.4 576,899 - COO64_RS05340 0.73 -0.9 576,899 - COO64_RS05340 0.73 -1.4 576,899 - COO64_RS05340 0.73 +0.2 576,899 - COO64_RS05340 0.73 -0.5 576,899 - COO64_RS05340 0.73 +0.2 576,931 + COO64_RS05340 0.75 +0.2 576,931 + COO64_RS05340 0.75 +0.9 576,931 + COO64_RS05340 0.75 +0.3 576,952 + COO64_RS05340 0.76 +0.2 576,952 + COO64_RS05340 0.76 +0.4 576,952 + COO64_RS05340 0.76 +0.3 576,953 - COO64_RS05340 0.76 -0.0 576,991 + COO64_RS05340 0.78 +0.3 576,991 + COO64_RS05340 0.78 -0.2 576,991 + COO64_RS05340 0.78 +0.5 576,992 - COO64_RS05340 0.78 -0.9 576,992 - COO64_RS05340 0.78 +0.2 576,992 - COO64_RS05340 0.78 +0.7 576,992 - COO64_RS05340 0.78 +0.1 576,992 - COO64_RS05340 0.78 -0.2 577,045 + COO64_RS05340 0.80 +1.1 577,045 + COO64_RS05340 0.80 -0.8 577,045 + COO64_RS05340 0.80 +1.4 577,045 + COO64_RS05340 0.80 -0.8 577,045 + COO64_RS05340 0.80 -0.3 577,045 + COO64_RS05340 0.80 +0.5 577,045 + COO64_RS05340 0.80 -0.2 577,045 + COO64_RS05340 0.80 +1.7 577,045 + COO64_RS05340 0.80 -1.0 577,045 + COO64_RS05340 0.80 -0.1 577,045 + COO64_RS05340 0.80 -0.4 577,045 + COO64_RS05340 0.80 +1.6 577,045 + COO64_RS05340 0.80 -0.1 577,045 + COO64_RS05340 0.80 +0.3 577,045 + COO64_RS05340 0.80 +0.6 577,046 - COO64_RS05340 0.80 -0.3 577,046 - COO64_RS05340 0.80 -0.3 577,046 - COO64_RS05340 0.80 +0.2 577,046 - COO64_RS05340 0.80 +0.6 577,046 - COO64_RS05340 0.80 +0.3 577,046 - COO64_RS05340 0.80 -2.1 577,046 - COO64_RS05340 0.80 -0.2 577,046 - COO64_RS05340 0.80 -1.2 577,046 - COO64_RS05340 0.80 -0.8 577,046 - COO64_RS05340 0.80 +0.8 577,046 - COO64_RS05340 0.80 +0.7 577,046 - COO64_RS05340 0.80 +0.6 577,087 + COO64_RS05340 0.82 +0.1 577,108 + COO64_RS05340 0.83 +0.3 577,312 + +0.7 577,312 + +0.1 577,312 + +0.2 577,312 + -0.6 577,312 + +0.5 577,312 + -0.8 577,312 + +0.1 577,312 + -0.7 577,312 + +0.6 577,312 + -0.7 577,312 + +0.9 577,312 + +0.5 577,312 + -0.1 577,312 + +0.0 577,312 + +0.7 577,313 - +1.2 577,313 - +0.1 577,313 - +0.1 577,313 - +0.7 577,313 - +0.2 577,313 - -0.4 577,313 - -0.5 577,313 - -0.0 577,313 - -0.1 577,313 - -0.2 577,313 - -0.2 577,313 - -0.1 577,313 - +0.3 577,313 - +1.2 577,313 - +0.6 577,313 - +1.1 577,313 - +0.6 577,495 - -0.3 577,511 + -0.9 577,527 + +1.1 577,527 + -0.3 577,528 - +0.2 577,528 - -0.3 577,545 - +0.9 577,545 - -1.7 577,545 - -1.0 577,545 - -0.2 577,545 - -0.1 577,545 - +1.2 577,621 - +1.5 577,656 + COO64_RS05345 0.13 -1.4 577,716 + COO64_RS05345 0.18 +0.0 577,717 - COO64_RS05345 0.18 +0.6 577,717 - COO64_RS05345 0.18 -0.7 577,773 + COO64_RS05345 0.24 +1.1 577,872 - COO64_RS05345 0.33 -1.1 577,872 - COO64_RS05345 0.33 -0.8 577,872 - COO64_RS05345 0.33 +1.7 577,872 - COO64_RS05345 0.33 +0.3 578,091 + COO64_RS05345 0.54 -0.4 578,091 + COO64_RS05345 0.54 -1.2 578,091 + COO64_RS05345 0.54 -1.7 578,091 + COO64_RS05345 0.54 +0.5 578,091 + COO64_RS05345 0.54 -0.2 578,092 - COO64_RS05345 0.55 +1.0 578,092 - COO64_RS05345 0.55 +0.9 578,092 - COO64_RS05345 0.55 -0.4 578,092 - COO64_RS05345 0.55 +0.1 578,092 - COO64_RS05345 0.55 -0.9 578,092 - COO64_RS05345 0.55 -0.6 578,092 - COO64_RS05345 0.55 -1.7 578,092 - COO64_RS05345 0.55 -1.0 578,092 - COO64_RS05345 0.55 -0.2 578,136 + COO64_RS05345 0.59 +0.1 578,136 + COO64_RS05345 0.59 -1.5 578,136 + COO64_RS05345 0.59 -1.2 578,137 - COO64_RS05345 0.59 -2.7 578,137 - COO64_RS05345 0.59 -0.9 578,137 - COO64_RS05345 0.59 -0.5 578,137 - COO64_RS05345 0.59 +0.7 578,235 + COO64_RS05345 0.68 +0.5 578,236 - COO64_RS05345 0.68 +0.5 578,271 + COO64_RS05345 0.72 +0.0 578,271 + COO64_RS05345 0.72 -0.7 578,271 + COO64_RS05345 0.72 -0.6 578,272 - COO64_RS05345 0.72 -1.1 578,385 + COO64_RS05345 0.83 -0.2 578,385 + COO64_RS05345 0.83 +0.7 578,386 - COO64_RS05345 0.83 +0.5 578,442 + COO64_RS05345 0.88 -0.9 578,442 + COO64_RS05345 0.88 -1.1 578,442 + COO64_RS05345 0.88 -0.7 578,443 - COO64_RS05345 0.88 +0.2 578,443 - COO64_RS05345 0.88 -0.5 578,443 - COO64_RS05345 0.88 +0.9 578,446 - COO64_RS05345 0.89 -1.8 578,446 - COO64_RS05345 0.89 +0.6 578,520 - -0.4 578,568 + +0.3 578,586 + -0.7 578,586 + -0.1 578,586 + +0.1 578,587 - +1.3 578,587 - -0.8 578,587 - -0.4 578,587 - +0.9 578,587 - +0.2 578,590 + -0.0 578,590 + +0.8 578,590 + -0.3 578,590 + -0.7 578,591 - +0.4 578,591 - -1.4 578,591 - -0.2 578,591 - -0.7 578,634 + +0.4 578,655 + -0.5 578,655 + -0.4 578,661 + -1.4 578,661 + +0.3 578,661 + -0.2 578,662 - +0.0 578,662 - -0.4 578,662 - -0.5 578,662 - -0.7 578,676 - +0.5 578,708 - -0.1 578,846 - sigX COO64_RS05350 0.30 -0.4 578,984 + sigX COO64_RS05350 0.55 -0.7 579,234 - +0.3 579,258 + -0.7 579,302 + +0.9 579,333 + -0.9 579,334 - -0.2 579,334 - -0.1 579,334 - +1.7 579,334 - -1.3 579,334 - +1.8 579,334 - -0.7 579,334 - -0.7 579,339 - -1.4 579,339 - +0.3 579,363 + +0.3 579,444 + COO64_RS05355 0.11 -0.2 579,503 + COO64_RS05355 0.18 +0.7
Or see this region's nucleotide sequence