Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS05220 and COO64_RS05225 are separated by 50 nucleotides COO64_RS05225 and COO64_RS05230 are separated by 92 nucleotides COO64_RS05230 and COO64_RS05235 are separated by 94 nucleotides COO64_RS05235 and COO64_RS05240 are separated by 178 nucleotides
COO64_RS05220: COO64_RS05220 - pseudouridine synthase, at 545,336 to 546,223
_RS05220
COO64_RS05225: COO64_RS05225 - hypothetical protein, at 546,274 to 546,585
_RS05225
COO64_RS05230: COO64_RS05230 - D-Ala-D-Ala carboxypeptidase family metallohydrolase, at 546,678 to 547,109
_RS05230
COO64_RS05235: COO64_RS05235 - glutaredoxin family protein, at 547,204 to 547,440
_RS05235
COO64_RS05240: COO64_RS05240 - monovalent cation:proton antiporter family protein, at 547,619 to 549,577
_RS05240
Position (kb)
547
548 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 546.306 kb on - strand, within COO64_RS05225 at 546.306 kb on - strand, within COO64_RS05225 at 546.356 kb on + strand, within COO64_RS05225 at 546.356 kb on + strand, within COO64_RS05225 at 546.357 kb on - strand, within COO64_RS05225 at 546.366 kb on - strand, within COO64_RS05225 at 546.450 kb on + strand, within COO64_RS05225 at 546.450 kb on + strand, within COO64_RS05225 at 546.450 kb on + strand, within COO64_RS05225 at 546.450 kb on + strand, within COO64_RS05225 at 546.450 kb on + strand, within COO64_RS05225 at 546.451 kb on - strand, within COO64_RS05225 at 546.451 kb on - strand, within COO64_RS05225 at 546.560 kb on + strand at 546.625 kb on - strand at 546.631 kb on - strand at 546.710 kb on - strand at 546.751 kb on - strand, within COO64_RS05230 at 546.881 kb on + strand, within COO64_RS05230 at 546.881 kb on + strand, within COO64_RS05230 at 546.882 kb on - strand, within COO64_RS05230 at 546.882 kb on - strand, within COO64_RS05230 at 546.882 kb on - strand, within COO64_RS05230 at 547.034 kb on + strand, within COO64_RS05230 at 547.034 kb on + strand, within COO64_RS05230 at 547.035 kb on - strand, within COO64_RS05230 at 547.035 kb on - strand, within COO64_RS05230 at 547.205 kb on + strand at 547.205 kb on + strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.206 kb on - strand at 547.287 kb on + strand, within COO64_RS05235 at 547.287 kb on + strand, within COO64_RS05235 at 547.299 kb on - strand, within COO64_RS05235 at 547.299 kb on - strand, within COO64_RS05235 at 547.299 kb on - strand, within COO64_RS05235 at 547.299 kb on - strand, within COO64_RS05235 at 547.299 kb on - strand, within COO64_RS05235 at 547.329 kb on + strand, within COO64_RS05235 at 547.442 kb on + strand at 547.442 kb on + strand at 547.442 kb on + strand at 547.442 kb on + strand at 547.442 kb on + strand at 547.443 kb on - strand at 547.443 kb on - strand at 547.443 kb on - strand at 547.443 kb on - strand at 547.443 kb on - strand at 547.460 kb on + strand at 547.461 kb on - strand at 547.474 kb on + strand at 547.474 kb on + strand at 547.475 kb on - strand at 547.475 kb on - strand at 547.475 kb on - strand at 547.479 kb on + strand at 547.479 kb on + strand at 547.480 kb on - strand at 547.480 kb on - strand at 547.480 kb on - strand at 547.582 kb on + strand at 547.582 kb on + strand at 547.583 kb on - strand at 547.669 kb on + strand at 547.669 kb on + strand at 547.669 kb on + strand at 547.706 kb on + strand at 547.706 kb on + strand at 547.706 kb on + strand at 547.707 kb on - strand at 547.707 kb on - strand at 547.735 kb on + strand at 547.959 kb on + strand, within COO64_RS05240 at 548.138 kb on + strand, within COO64_RS05240 at 548.139 kb on - strand, within COO64_RS05240 at 548.375 kb on + strand, within COO64_RS05240 at 548.375 kb on + strand, within COO64_RS05240 at 548.375 kb on + strand, within COO64_RS05240 at 548.375 kb on + strand, within COO64_RS05240 at 548.375 kb on + strand, within COO64_RS05240 at 548.376 kb on - strand, within COO64_RS05240 at 548.376 kb on - strand, within COO64_RS05240 at 548.376 kb on - strand, within COO64_RS05240
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 546,306 - COO64_RS05225 0.10 -0.8 546,306 - COO64_RS05225 0.10 -0.4 546,356 + COO64_RS05225 0.26 -0.4 546,356 + COO64_RS05225 0.26 -0.3 546,357 - COO64_RS05225 0.27 -0.0 546,366 - COO64_RS05225 0.29 -0.1 546,450 + COO64_RS05225 0.56 +0.3 546,450 + COO64_RS05225 0.56 -0.7 546,450 + COO64_RS05225 0.56 +0.0 546,450 + COO64_RS05225 0.56 +0.9 546,450 + COO64_RS05225 0.56 -1.4 546,451 - COO64_RS05225 0.57 +0.5 546,451 - COO64_RS05225 0.57 +0.5 546,560 + +1.6 546,625 - -1.6 546,631 - -0.5 546,710 - +0.0 546,751 - COO64_RS05230 0.17 -0.9 546,881 + COO64_RS05230 0.47 -0.7 546,881 + COO64_RS05230 0.47 +0.8 546,882 - COO64_RS05230 0.47 +1.7 546,882 - COO64_RS05230 0.47 -0.2 546,882 - COO64_RS05230 0.47 -0.8 547,034 + COO64_RS05230 0.82 +0.8 547,034 + COO64_RS05230 0.82 +0.8 547,035 - COO64_RS05230 0.83 -1.2 547,035 - COO64_RS05230 0.83 -0.8 547,205 + -0.2 547,205 + -1.2 547,206 - +1.9 547,206 - -0.4 547,206 - +0.2 547,206 - -0.8 547,206 - -0.7 547,206 - -0.8 547,206 - -0.9 547,287 + COO64_RS05235 0.35 -2.0 547,287 + COO64_RS05235 0.35 -0.5 547,299 - COO64_RS05235 0.40 -1.8 547,299 - COO64_RS05235 0.40 -1.2 547,299 - COO64_RS05235 0.40 -0.2 547,299 - COO64_RS05235 0.40 -0.8 547,299 - COO64_RS05235 0.40 -1.1 547,329 + COO64_RS05235 0.53 -0.1 547,442 + -0.4 547,442 + -1.4 547,442 + -1.1 547,442 + +0.4 547,442 + -0.0 547,443 - -0.8 547,443 - -0.9 547,443 - +0.3 547,443 - -1.4 547,443 - +0.7 547,460 + -0.1 547,461 - +0.4 547,474 + +0.1 547,474 + -0.7 547,475 - -0.7 547,475 - -0.3 547,475 - -0.1 547,479 + -0.1 547,479 + +0.9 547,480 - +1.3 547,480 - -1.0 547,480 - +0.2 547,582 + +0.3 547,582 + +0.7 547,583 - -2.3 547,669 + -0.5 547,669 + +0.0 547,669 + -0.2 547,706 + +0.2 547,706 + +0.8 547,706 + +0.3 547,707 - +0.0 547,707 - -0.6 547,735 + +0.4 547,959 + COO64_RS05240 0.17 -0.9 548,138 + COO64_RS05240 0.26 -0.5 548,139 - COO64_RS05240 0.27 +0.6 548,375 + COO64_RS05240 0.39 -0.1 548,375 + COO64_RS05240 0.39 -0.1 548,375 + COO64_RS05240 0.39 -0.3 548,375 + COO64_RS05240 0.39 +0.4 548,375 + COO64_RS05240 0.39 -0.1 548,376 - COO64_RS05240 0.39 -0.3 548,376 - COO64_RS05240 0.39 +0.9 548,376 - COO64_RS05240 0.39 +1.5
Or see this region's nucleotide sequence