Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt COO64_RS04945 and COO64_RS04950 are separated by 84 nucleotides COO64_RS04950 and COO64_RS04955 are separated by 7 nucleotides COO64_RS04955 and COO64_RS04960 are separated by 150 nucleotides
COO64_RS04945: COO64_RS04945 - transporter substrate-binding domain-containing protein, at 481,407 to 485,003
_RS04945
COO64_RS04950: COO64_RS04950 - EAL domain-containing protein, at 485,088 to 486,308
_RS04950
COO64_RS04955: COO64_RS04955 - response regulator transcription factor, at 486,316 to 486,975
_RS04955
COO64_RS04960: COO64_RS04960 - DUF3509 domain-containing protein, at 487,126 to 487,413
_RS04960
Position (kb)
485
486
487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 484.128 kb on - strand, within COO64_RS04945 at 484.128 kb on - strand, within COO64_RS04945 at 484.128 kb on - strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.166 kb on + strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.167 kb on - strand, within COO64_RS04945 at 484.328 kb on - strand, within COO64_RS04945 at 484.461 kb on + strand, within COO64_RS04945 at 484.462 kb on - strand, within COO64_RS04945 at 484.538 kb on + strand, within COO64_RS04945 at 484.695 kb on - strand at 484.730 kb on - strand at 484.901 kb on + strand at 484.902 kb on - strand at 484.902 kb on - strand at 484.902 kb on - strand at 484.964 kb on + strand at 484.965 kb on - strand at 485.001 kb on + strand at 485.002 kb on - strand at 485.002 kb on - strand at 485.002 kb on - strand at 485.013 kb on + strand at 485.014 kb on - strand at 485.099 kb on + strand at 485.099 kb on + strand at 485.099 kb on + strand at 485.099 kb on + strand at 485.100 kb on - strand at 485.100 kb on - strand at 485.100 kb on - strand at 485.100 kb on - strand at 485.100 kb on - strand at 485.574 kb on + strand, within COO64_RS04950 at 485.574 kb on + strand, within COO64_RS04950 at 486.216 kb on + strand at 486.216 kb on + strand at 486.216 kb on + strand at 486.216 kb on + strand at 486.216 kb on + strand at 486.217 kb on - strand at 486.217 kb on - strand at 486.217 kb on - strand at 486.244 kb on + strand at 486.244 kb on + strand at 486.244 kb on + strand at 486.245 kb on - strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.339 kb on + strand at 486.340 kb on - strand at 486.340 kb on - strand at 486.340 kb on - strand at 486.340 kb on - strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.341 kb on + strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.342 kb on - strand at 486.347 kb on + strand at 486.347 kb on + strand at 486.347 kb on + strand at 486.347 kb on + strand at 486.348 kb on - strand at 486.554 kb on - strand, within COO64_RS04955 at 486.554 kb on - strand, within COO64_RS04955 at 486.554 kb on - strand, within COO64_RS04955 at 486.578 kb on + strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.579 kb on - strand, within COO64_RS04955 at 486.721 kb on + strand, within COO64_RS04955 at 486.722 kb on - strand, within COO64_RS04955 at 486.742 kb on + strand, within COO64_RS04955 at 486.743 kb on - strand, within COO64_RS04955 at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.107 kb on + strand at 487.108 kb on - strand at 487.108 kb on - strand at 487.108 kb on - strand at 487.108 kb on - strand at 487.134 kb on + strand at 487.134 kb on + strand at 487.254 kb on + strand, within COO64_RS04960 at 487.255 kb on - strand, within COO64_RS04960 at 487.255 kb on - strand, within COO64_RS04960 at 487.260 kb on - strand, within COO64_RS04960 at 487.260 kb on - strand, within COO64_RS04960 at 487.260 kb on - strand, within COO64_RS04960 at 487.260 kb on - strand, within COO64_RS04960
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 484,128 - COO64_RS04945 0.76 -0.1 484,128 - COO64_RS04945 0.76 +1.3 484,128 - COO64_RS04945 0.76 +0.2 484,166 + COO64_RS04945 0.77 +0.2 484,166 + COO64_RS04945 0.77 -0.0 484,166 + COO64_RS04945 0.77 +0.2 484,166 + COO64_RS04945 0.77 -0.2 484,166 + COO64_RS04945 0.77 -1.1 484,166 + COO64_RS04945 0.77 +0.5 484,166 + COO64_RS04945 0.77 +0.4 484,166 + COO64_RS04945 0.77 -0.1 484,167 - COO64_RS04945 0.77 +0.0 484,167 - COO64_RS04945 0.77 -0.7 484,167 - COO64_RS04945 0.77 -1.9 484,167 - COO64_RS04945 0.77 +1.0 484,167 - COO64_RS04945 0.77 +1.0 484,167 - COO64_RS04945 0.77 -0.6 484,167 - COO64_RS04945 0.77 +0.7 484,167 - COO64_RS04945 0.77 +1.2 484,167 - COO64_RS04945 0.77 +0.2 484,167 - COO64_RS04945 0.77 -0.4 484,167 - COO64_RS04945 0.77 -0.5 484,328 - COO64_RS04945 0.81 -1.4 484,461 + COO64_RS04945 0.85 -0.7 484,462 - COO64_RS04945 0.85 -0.9 484,538 + COO64_RS04945 0.87 -1.9 484,695 - +1.4 484,730 - +0.4 484,901 + -0.2 484,902 - +0.1 484,902 - -0.6 484,902 - -0.0 484,964 + +0.2 484,965 - -1.3 485,001 + +0.2 485,002 - -0.4 485,002 - -0.6 485,002 - -0.2 485,013 + +0.5 485,014 - +0.2 485,099 + -0.3 485,099 + +0.3 485,099 + -1.2 485,099 + -1.4 485,100 - +0.1 485,100 - +0.4 485,100 - -0.5 485,100 - +0.4 485,100 - +0.4 485,574 + COO64_RS04950 0.40 -0.2 485,574 + COO64_RS04950 0.40 -0.8 486,216 + +0.5 486,216 + -0.3 486,216 + -0.6 486,216 + -0.3 486,216 + -0.4 486,217 - -0.9 486,217 - +0.3 486,217 - +0.2 486,244 + -0.4 486,244 + -0.5 486,244 + -0.4 486,245 - -0.1 486,339 + +0.2 486,339 + +0.3 486,339 + -0.6 486,339 + -0.4 486,339 + +1.0 486,339 + +0.7 486,339 + -1.2 486,340 - -0.4 486,340 - -0.1 486,340 - +0.9 486,340 - -0.6 486,341 + +2.0 486,341 + -0.2 486,341 + +0.4 486,341 + +0.7 486,341 + -1.0 486,341 + +0.0 486,341 + +0.4 486,341 + -1.5 486,341 + +1.0 486,341 + -0.4 486,341 + -0.5 486,341 + +0.2 486,341 + -0.6 486,342 - -0.4 486,342 - -0.7 486,342 - -0.3 486,342 - -2.8 486,342 - +0.3 486,342 - -2.0 486,342 - +0.1 486,342 - +0.2 486,342 - -0.7 486,342 - +0.3 486,342 - -0.3 486,342 - -0.4 486,342 - -0.7 486,342 - +0.2 486,342 - -0.8 486,342 - -0.2 486,347 + -1.1 486,347 + +1.2 486,347 + -0.8 486,347 + -0.5 486,348 - -1.4 486,554 - COO64_RS04955 0.36 +0.2 486,554 - COO64_RS04955 0.36 -0.7 486,554 - COO64_RS04955 0.36 -1.0 486,578 + COO64_RS04955 0.40 -0.7 486,579 - COO64_RS04955 0.40 +0.1 486,579 - COO64_RS04955 0.40 +0.8 486,579 - COO64_RS04955 0.40 +0.4 486,579 - COO64_RS04955 0.40 +1.0 486,579 - COO64_RS04955 0.40 -0.4 486,579 - COO64_RS04955 0.40 +0.2 486,579 - COO64_RS04955 0.40 +0.2 486,579 - COO64_RS04955 0.40 -0.7 486,721 + COO64_RS04955 0.61 +0.2 486,722 - COO64_RS04955 0.62 -1.0 486,742 + COO64_RS04955 0.65 -0.1 486,743 - COO64_RS04955 0.65 +1.0 487,107 + +0.7 487,107 + -0.4 487,107 + -1.0 487,107 + +0.7 487,107 + +0.2 487,107 + -0.5 487,107 + -0.7 487,107 + +0.2 487,108 - -0.2 487,108 - -0.0 487,108 - +0.3 487,108 - +0.6 487,134 + +0.2 487,134 + -1.1 487,254 + COO64_RS04960 0.44 +1.1 487,255 - COO64_RS04960 0.45 -1.2 487,255 - COO64_RS04960 0.45 -0.7 487,260 - COO64_RS04960 0.47 +0.1 487,260 - COO64_RS04960 0.47 +0.5 487,260 - COO64_RS04960 0.47 -0.6 487,260 - COO64_RS04960 0.47 +0.3
Or see this region's nucleotide sequence