Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt zwf and pgl overlap by 14 nucleotides pgl and COO64_RS04600 are separated by 17 nucleotides COO64_RS04600 and COO64_RS04605 are separated by 174 nucleotides
COO64_RS04590: zwf - glucose-6-phosphate dehydrogenase, at 399,274 to 400,743
zwf
COO64_RS04595: pgl - 6-phosphogluconolactonase, at 400,730 to 401,443
pgl
COO64_RS04600: COO64_RS04600 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 401,461 to 402,135
_RS04600
COO64_RS04605: COO64_RS04605 - monalysin family beta-barrel pore-forming toxin, at 402,310 to 403,044
_RS04605
Position (kb) 400
401
402 Strain fitness (log2 ratio) -2
-1
0
1
2 at 399.763 kb on + strand, within zwf at 399.764 kb on - strand, within zwf at 399.764 kb on - strand, within zwf at 399.784 kb on + strand, within zwf at 399.799 kb on + strand, within zwf at 399.799 kb on + strand, within zwf at 399.799 kb on + strand, within zwf at 399.799 kb on + strand, within zwf at 399.800 kb on - strand, within zwf at 399.800 kb on - strand, within zwf at 399.941 kb on - strand, within zwf at 400.045 kb on - strand, within zwf at 400.129 kb on + strand, within zwf at 400.129 kb on + strand, within zwf at 400.129 kb on + strand, within zwf at 400.130 kb on - strand, within zwf at 400.130 kb on - strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.339 kb on + strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.340 kb on - strand, within zwf at 400.410 kb on + strand, within zwf at 400.410 kb on + strand, within zwf at 400.411 kb on - strand, within zwf at 400.411 kb on - strand, within zwf at 400.411 kb on - strand, within zwf at 400.411 kb on - strand, within zwf at 400.411 kb on - strand, within zwf at 400.529 kb on - strand, within zwf at 400.529 kb on - strand, within zwf at 400.729 kb on + strand at 400.730 kb on - strand at 400.852 kb on + strand, within pgl at 400.852 kb on + strand, within pgl at 401.139 kb on + strand, within pgl at 401.139 kb on + strand, within pgl at 401.409 kb on + strand at 401.409 kb on + strand at 401.409 kb on + strand at 401.410 kb on - strand at 401.410 kb on - strand at 401.461 kb on - strand at 401.870 kb on - strand, within COO64_RS04600 at 402.010 kb on + strand, within COO64_RS04600 at 402.010 kb on + strand, within COO64_RS04600 at 402.010 kb on + strand, within COO64_RS04600 at 402.011 kb on - strand, within COO64_RS04600 at 402.011 kb on - strand, within COO64_RS04600 at 402.011 kb on - strand, within COO64_RS04600 at 402.011 kb on - strand, within COO64_RS04600 at 402.011 kb on - strand, within COO64_RS04600 at 402.046 kb on - strand, within COO64_RS04600 at 402.155 kb on + strand at 402.271 kb on - strand at 402.276 kb on + strand at 402.276 kb on + strand at 402.277 kb on - strand at 402.295 kb on + strand at 402.295 kb on + strand at 402.309 kb on + strand at 402.326 kb on + strand at 402.326 kb on + strand at 402.326 kb on + strand at 402.326 kb on + strand at 402.327 kb on - strand at 402.365 kb on - strand at 402.365 kb on - strand at 402.365 kb on - strand at 402.379 kb on + strand at 402.379 kb on + strand at 402.379 kb on + strand at 402.380 kb on - strand at 402.380 kb on - strand at 402.380 kb on - strand at 402.380 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 399,763 + zwf COO64_RS04590 0.33 -1.6 399,764 - zwf COO64_RS04590 0.33 -1.8 399,764 - zwf COO64_RS04590 0.33 -0.3 399,784 + zwf COO64_RS04590 0.35 -2.2 399,799 + zwf COO64_RS04590 0.36 -0.3 399,799 + zwf COO64_RS04590 0.36 +0.5 399,799 + zwf COO64_RS04590 0.36 -0.3 399,799 + zwf COO64_RS04590 0.36 -1.0 399,800 - zwf COO64_RS04590 0.36 -1.0 399,800 - zwf COO64_RS04590 0.36 -0.8 399,941 - zwf COO64_RS04590 0.45 -0.2 400,045 - zwf COO64_RS04590 0.52 -1.8 400,129 + zwf COO64_RS04590 0.58 +1.1 400,129 + zwf COO64_RS04590 0.58 +0.5 400,129 + zwf COO64_RS04590 0.58 +0.1 400,130 - zwf COO64_RS04590 0.58 -0.7 400,130 - zwf COO64_RS04590 0.58 -0.8 400,339 + zwf COO64_RS04590 0.72 -0.1 400,339 + zwf COO64_RS04590 0.72 +1.3 400,339 + zwf COO64_RS04590 0.72 -1.6 400,339 + zwf COO64_RS04590 0.72 +0.2 400,339 + zwf COO64_RS04590 0.72 -0.2 400,339 + zwf COO64_RS04590 0.72 -0.3 400,339 + zwf COO64_RS04590 0.72 -0.3 400,340 - zwf COO64_RS04590 0.73 -0.7 400,340 - zwf COO64_RS04590 0.73 +0.2 400,340 - zwf COO64_RS04590 0.73 -1.2 400,340 - zwf COO64_RS04590 0.73 -1.6 400,340 - zwf COO64_RS04590 0.73 -0.6 400,340 - zwf COO64_RS04590 0.73 -0.8 400,340 - zwf COO64_RS04590 0.73 +0.4 400,340 - zwf COO64_RS04590 0.73 -1.4 400,340 - zwf COO64_RS04590 0.73 -0.6 400,340 - zwf COO64_RS04590 0.73 -1.8 400,340 - zwf COO64_RS04590 0.73 -0.9 400,340 - zwf COO64_RS04590 0.73 -0.3 400,410 + zwf COO64_RS04590 0.77 -0.5 400,410 + zwf COO64_RS04590 0.77 +0.3 400,411 - zwf COO64_RS04590 0.77 -0.1 400,411 - zwf COO64_RS04590 0.77 +0.2 400,411 - zwf COO64_RS04590 0.77 +0.0 400,411 - zwf COO64_RS04590 0.77 -0.4 400,411 - zwf COO64_RS04590 0.77 +0.0 400,529 - zwf COO64_RS04590 0.85 -1.2 400,529 - zwf COO64_RS04590 0.85 +0.4 400,729 + -0.4 400,730 - -1.6 400,852 + pgl COO64_RS04595 0.17 -0.6 400,852 + pgl COO64_RS04595 0.17 -1.0 401,139 + pgl COO64_RS04595 0.57 -0.1 401,139 + pgl COO64_RS04595 0.57 -0.8 401,409 + -0.6 401,409 + +0.4 401,409 + -0.3 401,410 - +0.4 401,410 - -0.8 401,461 - -0.2 401,870 - COO64_RS04600 0.61 +0.8 402,010 + COO64_RS04600 0.81 +0.0 402,010 + COO64_RS04600 0.81 +2.0 402,010 + COO64_RS04600 0.81 -0.3 402,011 - COO64_RS04600 0.81 -1.0 402,011 - COO64_RS04600 0.81 -1.3 402,011 - COO64_RS04600 0.81 +0.0 402,011 - COO64_RS04600 0.81 -1.4 402,011 - COO64_RS04600 0.81 -0.6 402,046 - COO64_RS04600 0.87 -1.3 402,155 + +0.0 402,271 - -0.7 402,276 + +0.6 402,276 + -0.1 402,277 - +0.4 402,295 + +0.8 402,295 + +0.5 402,309 + +0.7 402,326 + -2.3 402,326 + -0.9 402,326 + -0.1 402,326 + +0.3 402,327 - +0.4 402,365 - +0.9 402,365 - -0.8 402,365 - +1.4 402,379 + -0.3 402,379 + +0.7 402,379 + +0.1 402,380 - -0.3 402,380 - +0.5 402,380 - -0.8 402,380 - +1.5
Or see this region's nucleotide sequence