Experiment: supernatant control, M9 with 0.25X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lptB and lptA are separated by 0 nucleotides lptA and lptC overlap by 14 nucleotides lptC and COO64_RS04305 are separated by 8 nucleotides COO64_RS04305 and COO64_RS04310 are separated by 0 nucleotides
COO64_RS04290: lptB - LPS export ABC transporter ATP-binding protein, at 334,385 to 335,110
lptB
COO64_RS04295: lptA - lipopolysaccharide transport periplasmic protein LptA, at 335,111 to 335,635
lptA
COO64_RS04300: lptC - LPS export ABC transporter periplasmic protein LptC, at 335,622 to 336,197
lptC
COO64_RS04305: COO64_RS04305 - HAD family hydrolase, at 336,206 to 336,730
_RS04305
COO64_RS04310: COO64_RS04310 - KpsF/GutQ family sugar-phosphate isomerase, at 336,731 to 337,705
_RS04310
Position (kb) 335
336
337 Strain fitness (log2 ratio) -2
-1
0
1 at 335.706 kb on - strand, within lptC at 335.706 kb on - strand, within lptC at 335.706 kb on - strand, within lptC at 335.710 kb on - strand, within lptC at 335.710 kb on - strand, within lptC at 335.710 kb on - strand, within lptC at 335.756 kb on - strand, within lptC at 335.756 kb on - strand, within lptC at 335.756 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 335.924 kb on - strand, within lptC at 336.208 kb on - strand at 336.208 kb on - strand at 336.214 kb on - strand at 336.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction supernatant control, M9 with 0.25X LB remove 335,706 - lptC COO64_RS04300 0.15 +0.0 335,706 - lptC COO64_RS04300 0.15 -0.3 335,706 - lptC COO64_RS04300 0.15 -0.8 335,710 - lptC COO64_RS04300 0.15 -0.6 335,710 - lptC COO64_RS04300 0.15 -0.2 335,710 - lptC COO64_RS04300 0.15 +0.1 335,756 - lptC COO64_RS04300 0.23 -0.8 335,756 - lptC COO64_RS04300 0.23 -2.0 335,756 - lptC COO64_RS04300 0.23 -1.2 335,924 - lptC COO64_RS04300 0.52 -1.7 335,924 - lptC COO64_RS04300 0.52 +0.0 335,924 - lptC COO64_RS04300 0.52 -0.8 335,924 - lptC COO64_RS04300 0.52 -0.6 335,924 - lptC COO64_RS04300 0.52 -0.2 335,924 - lptC COO64_RS04300 0.52 -1.1 336,208 - +0.7 336,208 - +0.2 336,214 - -1.4 336,214 - -1.9
Or see this region's nucleotide sequence